MD16G1065800.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004492
Physical Location & Seq
Forward (+)
4611602 .. 4613338
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1065800.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGAGAAAGCTCTGACGAAAGTGAACAGCTTGAAAGTTGGGAGCTTGTGGATCTCGAAGAAGGCCAAGGAAGAATTCTCCAACATCTCCGACGACCTCTCTACATTCTCGAATACCGTCGAGGAGAAGGCAAAATGGGTTTTCAACAAACTAAAAGGAAAGCCGCCGAAAAGCTTGCCAGATCTCCTTCGAGAGTACAACTTGCCACCGGGGCTGTTTCCGCAGAACATTACCTGTTATGAGTTTGACGAAACAAAATCCAAGCTCATTGTGTATATGCCCTCAGCATGTGAAGTTAGCTTCAAGGATTCCTCTGTTATGAGGTACGCGACACGGGTAAAGGCCATTCTTCTAAGGGGAAAGCTCACGGGGATAGAGGGAATGAAGACCAAGGTTCTGGTATGGGTTAAGGTCACTTGTGTAGTGGTTGAAAGTTCCAAGTCCGATAAGGTGTGGTTTACAGCTGGTGTTAAGAAGTCAAGACCAAAGGATGTCTACATAACACCGCGTGACTCCGTGAAAGTAGAAGAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

179

Amino Acids

20.19

Weight (kDa)

9.52

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 61 - 166 2.8e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 495
AccII CGCG 2 cut(s) 329, 508
AciI CCGC 3 cut(s) 164, 221, 506
AclWI GGATC 1 cut(s) 59
AcsI RAATTY 2 cut(s) 74, 530
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 2 cut(s) 197, 326
AgsI TTSAA 4 cut(s) 34, 145, 304, 431
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 8 cut(s) 11, 30, 45, 174, 265, 300, 364, 464
AluI AGCT 8 cut(s) 11, 30, 45, 174, 265, 300, 364, 464
AlwI GGATC 1 cut(s) 59
AoxI GGCC 2 cut(s) 63, 342
ApoI RAATTY 2 cut(s) 74, 530
AsuC2I CCSGG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 392
BbvCI CCTCAGC 1 cut(s) 283
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BcnI CCSGG 1 cut(s) 210
BglI GCCNNNNNGGC 1 cut(s) 211
BglII AGATCT 1 cut(s) 181
BisI GCNGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 165
Bme1390I CCNGG 1 cut(s) 210
BmrFI CCNGG 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 283
BpuMI CCSGG 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 66, 209, 390
BseDI CCNNGG 3 cut(s) 66, 209, 390
BseGI GGATG 1 cut(s) 496
BseMII CTCAG 1 cut(s) 297
BseRI GAGGAG 1 cut(s) 137
Bsh1236I CGCG 2 cut(s) 329, 508
BshFI GGCC 2 cut(s) 65, 344
BsiSI CCGG 1 cut(s) 209
BsnI GGCC 2 cut(s) 65, 344
Bsp143I GATC 2 cut(s) 51, 181
BspACI CCGC 3 cut(s) 164, 221, 506
BspANI GGCC 2 cut(s) 65, 344
BspCNI CTCAG 1 cut(s) 296
BspFNI CGCG 2 cut(s) 329, 508
BspPI GGATC 1 cut(s) 59
BssECI CCNNGG 3 cut(s) 66, 209, 390
BssMI GATC 2 cut(s) 51, 181
BssT1I CCWWGG 2 cut(s) 66, 390
Bst4CI ACNGT 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 176
BstDEI CTNAG 2 cut(s) 283, 353
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 2 cut(s) 329, 508
BstKTI GATC 2 cut(s) 54, 184
BstMBI GATC 2 cut(s) 51, 181
BstMWI GCNNNNNNNGC 2 cut(s) 211, 220
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 208
BstUI CGCG 2 cut(s) 329, 508
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 2 cut(s) 51, 181
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 2 cut(s) 51, 181
BsuRI GGCC 2 cut(s) 65, 344
BtsCI GGATG 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 176
Csp6I GTAC 2 cut(s) 196, 325
CviAII CATG 1 cut(s) 288
CviQI GTAC 2 cut(s) 196, 325
DdeI CTNAG 2 cut(s) 283, 353
DpnI GATC 2 cut(s) 53, 183
DpnII GATC 2 cut(s) 51, 181
DraIII CACNNNGTG 1 cut(s) 509
Eco130I CCWWGG 2 cut(s) 66, 390
EcoRI GAATTC 2 cut(s) 74, 530
EcoT14I CCWWGG 2 cut(s) 66, 390
ErhI CCWWGG 2 cut(s) 66, 390
FaeI CATG 1 cut(s) 291
FaiI YATR 7 cut(s) 240, 276, 278, 289, 320, 403, 500
FatI CATG 1 cut(s) 287
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 164
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 164
HaeIII GGCC 2 cut(s) 65, 344
HapII CCGG 1 cut(s) 209
Hin1II CATG 1 cut(s) 291
HindIII AAGCTT 1 cut(s) 172
HinfI GANTC 2 cut(s) 308, 512
HpaII CCGG 1 cut(s) 209
Hpy166II GTNNAC 3 cut(s) 25, 459, 496
Hpy188I TCNGA 4 cut(s) 15, 91, 445, 536
Hpy188III TCNNGA 4 cut(s) 55, 109, 191, 480
Hpy8I GTNNAC 3 cut(s) 25, 459, 496
Hpy99I CGWCG 2 cut(s) 95, 122
HpyAV CCTTC 3 cut(s) 55, 121, 197
HpyCH4III ACNGT 1 cut(s) 118
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 220
HpyF3I CTNAG 2 cut(s) 283, 353
Hsp92II CATG 1 cut(s) 291
Kzo9I GATC 2 cut(s) 51, 181
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 5 cut(s) 192, 222, 247, 383, 450
MaeIII GTNAC 2 cut(s) 412, 509
MalI GATC 2 cut(s) 53, 183
MboI GATC 2 cut(s) 51, 181
MboII GAAGA 4 cut(s) 70, 83, 341, 397
MflI RGATCY 2 cut(s) 51, 181
MluCI AATT 2 cut(s) 74, 530
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 2 cut(s) 105, 114
MnlI CCTC 6 cut(s) 107, 115, 292, 315, 322, 370
MseI TTAA 2 cut(s) 408, 471
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 210
MvnI CGCG 2 cut(s) 329, 508
MwoI GCNNNNNNNGC 2 cut(s) 211, 220
NciI CCSGG 1 cut(s) 210
NdeII GATC 2 cut(s) 51, 181
NlaIII CATG 1 cut(s) 291
NmuCI GTSAC 2 cut(s) 412, 509
NspI RCATGY 1 cut(s) 291
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 1 cut(s) 165
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PsuI RGATCY 2 cut(s) 51, 181
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 197, 326
RsaNI GTAC 2 cut(s) 196, 325
SaqAI TTAA 2 cut(s) 408, 471
SatI GCNGC 1 cut(s) 164
Sau3AI GATC 2 cut(s) 51, 181
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 210
Sse9I AATT 2 cut(s) 74, 530
SsiI CCGC 3 cut(s) 164, 221, 506
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 2 cut(s) 66, 390
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 4 cut(s) 56, 110, 120, 190
TasI AATT 2 cut(s) 74, 530
TatI WGTACW 1 cut(s) 195
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 408, 471
Tru9I TTAA 2 cut(s) 408, 471
TseFI GTSAC 2 cut(s) 412, 509
Tsp45I GTSAC 2 cut(s) 412, 509
TspDTI ATGAA 1 cut(s) 398
TspGWI ACGGA 1 cut(s) 505
XapI RAATTY 2 cut(s) 74, 530
XceI RCATGY 1 cut(s) 291
XmiI GTMKAC 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.