Rh4DG371200

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004492
Physical Location & Seq
Forward (+)
58992368 .. 58993655
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG371200.1

Sequence Viewer

Length: 537 bp
ATGGAGAAAGCTCTAACCAAAGTGAACAGCTTGAAAATTGGGAGCTCATGGATCTCAAAGAAAGCCAAGGAAGAGTTGTCTAACATCAGCGACGACATCACCACTTTCTCAAGTACCGTTGAGCAGAAGGCAAAATGGATTTTCGACAAGCTGAAAGGAAAGCCACCGAAGAGCTTACCAGAACTCCTCCGAGAGTACAACTTGCCACCAGGGCTCTTTCCAGCAAACATAACCTGTTATGAATTTACCGAGACAAATGCTAAGCTGATTGTGTATTTGCCGGCTCCATCTGAAGTTACCTTCAAGGATTCATCTGCTATAAGGTATGCAACCCGGGTAAAGGCAAATCTGTTGAGGGGAAAGCTCATAGGGATAGAGGGAATGAAGACAAAGGTTGTTGTATGGGTTAAGGTTACTTGTGTAGCAGTGGAAAGTTCCAAGTCTGACAAGGTCTGGTTTACTGCTGGTGTTAAGAAGTCAAGATCCAAGGATGTCTACATCACACCCCAAGATGCTATTAGGGTACAAGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

178

Amino Acids

19.96

Weight (kDa)

9.61

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 60 - 166 1.6e-26 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 495
AclWI GGATC 2 cut(s) 59, 477
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 3 cut(s) 115, 197, 525
AfiI CCNNNNNNNGG 1 cut(s) 340
AgsI TTSAA 2 cut(s) 34, 304
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 7 cut(s) 11, 30, 45, 151, 174, 265, 364
AluI AGCT 7 cut(s) 11, 30, 45, 151, 174, 265, 364
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 2 cut(s) 59, 477
Ama87I CYCGRG 1 cut(s) 333
ApoI RAATTY 1 cut(s) 242
AsuC2I CCSGG 2 cut(s) 334, 335
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 333
BaeI ACNNNNGTAYC 1 cut(s) 515
BanII GRGCYC 2 cut(s) 47, 216
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 47
BccI CCATC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 1 cut(s) 210
BcnI CCSGG 2 cut(s) 334, 335
BcoDI GTCTC 1 cut(s) 245
BglI GCCNNNNNGGC 1 cut(s) 211
BlpI GCTNAGC 1 cut(s) 261
Bme1390I CCNGG 3 cut(s) 210, 334, 335
BmeT110I CYCGRG 1 cut(s) 333
BmiI GGNNCC 1 cut(s) 285
BmrFI CCNGG 3 cut(s) 210, 334, 335
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 94
BpuMI CCSGG 2 cut(s) 334, 335
BsaJI CCNNGG 4 cut(s) 66, 209, 333, 486
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse118I RCCGGY 1 cut(s) 280
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 4 cut(s) 66, 209, 333, 486
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 2 cut(s) 281, 334
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 47, 216
Bsp143I GATC 2 cut(s) 51, 482
Bsp1720I GCTNAGC 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 285
BspPI GGATC 2 cut(s) 59, 477
BspQI GCTCTTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 4 cut(s) 66, 209, 333, 486
BssMI GATC 2 cut(s) 51, 482
BssT1I CCWWGG 2 cut(s) 66, 486
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 2 cut(s) 66, 164
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 261
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 2 cut(s) 54, 485
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 51, 482
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 3 cut(s) 208, 332, 333
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 2 cut(s) 51, 482
BstYI RGATCY 2 cut(s) 51, 482
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 282
Cfr10I RCCGGY 1 cut(s) 280
Cfr9I CCCGGG 1 cut(s) 333
Csp6I GTAC 3 cut(s) 114, 196, 524
CviAII CATG 1 cut(s) 48
CviQI GTAC 3 cut(s) 114, 196, 524
DdeI CTNAG 1 cut(s) 261
DpnI GATC 2 cut(s) 53, 484
DpnII GATC 2 cut(s) 51, 482
Eam1104I CTCTTC 2 cut(s) 66, 164
EarI CTCTTC 2 cut(s) 66, 164
Ecl136II GAGCTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 66, 486
Eco24I GRGCYC 2 cut(s) 47, 216
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 208
EcoT14I CCWWGG 2 cut(s) 66, 486
EcoT38I GRGCYC 2 cut(s) 47, 216
ErhI CCWWGG 2 cut(s) 66, 486
FaeI CATG 1 cut(s) 51
FaiI YATR 7 cut(s) 49, 230, 240, 320, 327, 368, 403
FatI CATG 1 cut(s) 47
FblI GTMKAC 1 cut(s) 495
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 2 cut(s) 47, 216
HapII CCGG 2 cut(s) 281, 334
Hin1II CATG 1 cut(s) 51
HinfI GANTC 1 cut(s) 308
HpaII CCGG 2 cut(s) 281, 334
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 3 cut(s) 25, 459, 496
Hpy188I TCNGA 3 cut(s) 191, 292, 445
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 3 cut(s) 25, 459, 496
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 121, 310
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 1 cut(s) 51
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
Kzo9I GATC 2 cut(s) 51, 482
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 42, 289
LpnPI CCDG 9 cut(s) 192, 195, 222, 234, 247, 294, 347, 439, 450
LweI GCATC 1 cut(s) 502
MaeIII GTNAC 2 cut(s) 295, 412
MalI GATC 2 cut(s) 53, 484
MboI GATC 2 cut(s) 51, 482
MboII GAAGA 3 cut(s) 83, 181, 397
MflI RGATCY 2 cut(s) 51, 482
MhlI GDGCHC 2 cut(s) 47, 216
MluCI AATT 2 cut(s) 36, 242
MnlI CCTC 3 cut(s) 197, 348, 370
MroNI GCCGGC 1 cut(s) 280
MseI TTAA 2 cut(s) 408, 471
MspI CCGG 2 cut(s) 281, 334
MspR9I CCNGG 3 cut(s) 210, 334, 335
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 211
NaeI GCCGGC 1 cut(s) 282
NciI CCSGG 2 cut(s) 334, 335
NdeII GATC 2 cut(s) 51, 482
NgoMIV GCCGGC 1 cut(s) 280
NlaIII CATG 1 cut(s) 51
NlaIV GGNNCC 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 308
PflFI GACNNNGTC 1 cut(s) 449
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 208
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 285
PsuI RGATCY 2 cut(s) 51, 482
PsyI GACNNNGTC 1 cut(s) 449
RsaI GTAC 3 cut(s) 115, 197, 525
RsaNI GTAC 3 cut(s) 114, 196, 524
SacI GAGCTC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 2 cut(s) 408, 471
Sau3AI GATC 2 cut(s) 51, 482
ScrFI CCNGG 3 cut(s) 210, 334, 335
SduI GDGCHC 2 cut(s) 47, 216
SfaNI GCATC 1 cut(s) 502
SmaI CCCGGG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 2 cut(s) 36, 242
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 208, 332, 333
StyI CCWWGG 2 cut(s) 66, 486
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 144
TasI AATT 2 cut(s) 36, 242
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 408, 471
Tru9I TTAA 2 cut(s) 408, 471
TscAI CASTG 1 cut(s) 432
TspDTI ATGAA 3 cut(s) 255, 300, 398
TspMI CCCGGG 1 cut(s) 333
TspRI CASTG 1 cut(s) 432
Tth111I GACNNNGTC 1 cut(s) 449
XapI RAATTY 1 cut(s) 242
XmaI CCCGGG 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.