FvH4_4g30580

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004492
Physical Location & Seq
Forward (+)
30121355 .. 30122628
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30580.t1

Sequence Viewer

Length: 537 bp
ATGGAGAAGGCTCTAACCAAAGTGAGCAGCTTGAAGATTGGGAACTCATGGATCTCCAAGAAAGCCAAGGAGGAATTGTCTAACATCAGCGACGACATCACTACTTTCTCAAGTACTGTTGAGCAGAAGGCAAAATGGATTTTCGATAAGCTAAAAGGAAAGCCAACAAAGAGCTTACCAGAACTCCTGCGAGAGTACAACTTGCCACCAGGGCTCTTTCCGGCAAACATAACCTGTTATGAGTTTACCGAGACAAACGCCAAGCTGATTGTATATTTGCCTGCTCCATCTGAAGTTACCTTCAAGGATTCATCTTCTATAAGGTATGCAACACGGGTAAAGGCAAATCTGTTGAGGGGAAAGCTCTTAGGAATAGAGGGAATGAAGACAAAAGTTGTTGTATGGGTTAAGGTTACTTGTGTAGCAGTGGAAAGTACCAAGTCTGACAAGGTCTGGTTTACTGCTGGCGTTAAGAAATCAAGATCCAAGGATGTCTACATCATGCCCCAAGATGCTATCAGAGTACAAGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

179

Amino Acids

20.03

Weight (kDa)

9.61

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 60 - 166 2.3e-26 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 495
AclWI GGATC 2 cut(s) 59, 477
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 4 cut(s) 115, 197, 436, 525
AgsI TTSAA 2 cut(s) 34, 304
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 5 cut(s) 30, 151, 174, 265, 364
AluI AGCT 5 cut(s) 30, 151, 174, 265, 364
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 2 cut(s) 59, 477
ApeKI GCWGC 1 cut(s) 27
BanII GRGCYC 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 39
BccI CCATC 1 cut(s) 295
BciT130I CCWGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 245
BglI GCCNNNNNGGC 1 cut(s) 211
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 210
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 94
BsaJI CCNNGG 3 cut(s) 66, 209, 486
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 168, 198
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 3 cut(s) 66, 209, 486
BseGI GGATG 1 cut(s) 496
BseXI GCAGC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 51, 482
BspPI GGATC 2 cut(s) 59, 477
BssECI CCNNGG 3 cut(s) 66, 209, 486
BssMI GATC 2 cut(s) 51, 482
BssT1I CCWWGG 2 cut(s) 66, 486
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 118
BstC8I GCNNGC 2 cut(s) 282, 466
BstDEI CTNAG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 2 cut(s) 54, 485
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 51, 482
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 2 cut(s) 51, 482
BstYI RGATCY 2 cut(s) 51, 482
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 2 cut(s) 282, 466
Csp6I GTAC 4 cut(s) 114, 196, 435, 524
CviAII CATG 2 cut(s) 48, 502
CviJI RGCY 9 cut(s) 11, 30, 65, 151, 163, 174, 214, 265, 364
CviKI_1 RGCY 9 cut(s) 11, 30, 65, 151, 163, 174, 214, 265, 364
CviQI GTAC 4 cut(s) 114, 196, 435, 524
DdeI CTNAG 1 cut(s) 367
DpnI GATC 2 cut(s) 53, 484
DpnII GATC 2 cut(s) 51, 482
Eco130I CCWWGG 2 cut(s) 66, 486
Eco24I GRGCYC 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 208
EcoT14I CCWWGG 2 cut(s) 66, 486
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 2 cut(s) 66, 486
FaeI CATG 2 cut(s) 51, 505
FaiI YATR 8 cut(s) 49, 230, 240, 274, 320, 327, 403, 503
FatI CATG 2 cut(s) 47, 501
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 28
HapII CCGG 1 cut(s) 221
Hin1II CATG 2 cut(s) 51, 505
HinfI GANTC 1 cut(s) 308
HpaII CCGG 1 cut(s) 221
Hpy166II GTNNAC 3 cut(s) 246, 459, 496
Hpy188I TCNGA 3 cut(s) 292, 445, 521
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 3 cut(s) 246, 459, 496
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 121, 310
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpyF3I CTNAG 1 cut(s) 367
Hsp92II CATG 2 cut(s) 51, 505
Kzo9I GATC 2 cut(s) 51, 482
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 9 cut(s) 192, 195, 200, 222, 234, 247, 294, 439, 450
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 1 cut(s) 502
MaeIII GTNAC 2 cut(s) 295, 412
MalI GATC 2 cut(s) 53, 484
MboI GATC 2 cut(s) 51, 482
MboII GAAGA 3 cut(s) 46, 306, 397
MflI RGATCY 2 cut(s) 51, 482
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 74
MnlI CCTC 3 cut(s) 64, 348, 370
MseI TTAA 2 cut(s) 408, 471
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 211
NdeII GATC 2 cut(s) 51, 482
NlaIII CATG 2 cut(s) 51, 505
PfeI GAWTC 1 cut(s) 308
PflFI GACNNNGTC 1 cut(s) 449
PkrI GCNGC 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 208
PspGI CCWGG 1 cut(s) 208
PsuI RGATCY 2 cut(s) 51, 482
PsyI GACNNNGTC 1 cut(s) 449
RsaI GTAC 4 cut(s) 115, 197, 436, 525
RsaNI GTAC 4 cut(s) 114, 196, 435, 524
SaqAI TTAA 2 cut(s) 408, 471
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 2 cut(s) 51, 482
ScaI AGTACT 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 1 cut(s) 502
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 2 cut(s) 66, 486
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 144
TasI AATT 1 cut(s) 74
TatI WGTACW 3 cut(s) 113, 195, 523
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 408, 471
Tru9I TTAA 2 cut(s) 408, 471
TscAI CASTG 1 cut(s) 432
TseI GCWGC 1 cut(s) 27
TspDTI ATGAA 2 cut(s) 300, 398
TspRI CASTG 1 cut(s) 432
Tth111I GACNNNGTC 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 495
ZrmI AGTACT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.