Rorug06G0446100

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004492
Physical Location & Seq
Reverse (-)
57706242 .. 57708722
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0446100.1

Sequence Viewer

Length: 1239 bp
ATGTCAGGGCTAGCTTGCAACGCCTGTAACAAAGAGCTCAAGGACGACACCGAGCAGAGGCTCCATTACAAGTCCGAATGGCACCGCTACAACCTCAAGCGCAAGGTAGCTGGGGTTCCTGGAGTGACAGAAGCATTGTTTCTTGCAAGACAAGCTGCACTTGCTCAGGAGAAAAATGGTGCAAATGAAACCCCAATGCTCTACAGCTGTGCTCTTTGTGGCAAAGGGTATAGAAGTTCCAAGGCTCATGCTGAGCATCTCAAATCACGAAGTCACATTATGCGCGCTTCTCAAGGGACTGGTGATGAAGAAGAAGACAAGGCAATAGTCAGGCCACTTCCGAGTCGTGCGAATAAAGCTCCCCCTAGGAGAGAGGAAATTGATGAGGAGAGTCACGAAAGTGAAGAAAGTGGAGATGAGTGGGAGGAGGTTGACCCTGAGGATGAGTTGGTTAGTGAGACTGCAAAATCTTTGACTGGTTTAAATGTAGATGGGCATGCTTCTAATGAGGATATGGATGAAGATGACGATGACTATGAGGAGTTGGACCCATCATGTTGCTTTATGTGCGATAAAGAGCATAAGACCATAGAGAGATGCATGATTCACATGCACAAACACCATGGATTCTTCATTCCTGATATTGAGTATTTGAAGGACCCGAAAGGTCTTCTTACTTATCTTGGTCTCAAGGTTAAAAGGGATTTCATGTGTCTGTACTGCAATGATAGGTGCCAACCCTTTAACAGTTTGGAAGCAGTTAGGAAGCATATGGTGGCAAAAAGCCACTGCAAGGTACACTATGGAGATGGTGGTGATGAAGAAGAAGCAGAGTTGGATGAGTTCTATGATTACAGCAGCAGTTATGTCGATGAGGCTGGCAAGCAACTAGTTGTATCAGGTGAGACGGCCAACAGTGTCGAACTTGGGAGTGGTGGTGCTGAGCTCATCATTACTACAAGATCTGATCATGGGATATCAAGCAAAGCACTTGGTTCCCGGGAATTTATGCGCTATTATCGCCAGAAACCACGCCCATCACCTAACCATATGGCCATCACAGCTGCAGTGGCTGCAAGGTACAAGAGCATGGGGTTGGCAACTGTGCAATCAAAGGAACGAATGGTGCAGCTGAAAGTACTGAAGGAAATGAGAAGATCAGGAGTCGAGGCCATGCGCAGTAAGATGGGAATGAAAAGCAATGTGATCCGAAACCTCCCCAAGAATGTTCCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

412

Amino Acids

46.47

Weight (kDa)

5.77

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_2 PF12756 187 - 289 3.1e-27 C2H2 type zinc-finger (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1178
AccB1I GGYRCC 2 cut(s) 81, 732
AccII CGCG 1 cut(s) 285
AciI CCGC 1 cut(s) 85
AclWI GGATC 1 cut(s) 1201
AcoI YGGCCR 2 cut(s) 909, 1053
AcsI RAATTY 1 cut(s) 1004
AcuI CTGAAG 1 cut(s) 1163
AfaI GTAC 4 cut(s) 719, 798, 1082, 1140
AfiI CCNNNNNNNGG 2 cut(s) 57, 793
AgsI TTSAA 1 cut(s) 655
AhlI ACTAGT 1 cut(s) 889
AjnI CCWGG 1 cut(s) 118
AleI CACNNNNGTG 1 cut(s) 399
AluBI AGCT 9 cut(s) 14, 37, 110, 155, 207, 359, 946, 1064, 1132
AluI AGCT 9 cut(s) 14, 37, 110, 155, 207, 359, 946, 1064, 1132
Alw21I GWGCWC 3 cut(s) 39, 214, 948
Alw26I GTCTC 3 cut(s) 452, 692, 899
AlwI GGATC 1 cut(s) 1201
AlwNI CAGNNNCTG 1 cut(s) 1073
Ama87I CYCGRG 1 cut(s) 999
AoxI GGCC 4 cut(s) 332, 909, 1053, 1170
ApeKI GCWGC 5 cut(s) 155, 858, 1064, 1073, 1129
ApoI RAATTY 1 cut(s) 1004
AspA2I CCTAGG 1 cut(s) 365
AspLEI GCGC 5 cut(s) 102, 285, 287, 1014, 1179
AspS9I GGNCC 2 cut(s) 547, 658
AsuC2I CCSGG 2 cut(s) 1000, 1001
AsuHPI GGTGA 4 cut(s) 314, 827, 914, 1032
AsuNHI GCTAGC 1 cut(s) 10
AvaI CYCGRG 1 cut(s) 999
AvaII GGWCC 2 cut(s) 547, 658
AvrII CCTAGG 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 438
BalI TGGCCA 1 cut(s) 1055
BanI GGYRCC 2 cut(s) 81, 732
BanII GRGCYC 2 cut(s) 39, 948
BbsI GAAGAC 2 cut(s) 321, 662
Bbv12I GWGCWC 3 cut(s) 39, 214, 948
BbvI GCAGC 5 cut(s) 142, 870, 1051, 1060, 1141
BccI CCATC 6 cut(s) 485, 559, 803, 1045, 1064, 1180
BceAI ACGGC 1 cut(s) 924
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 967
BcnI CCSGG 2 cut(s) 1000, 1001
BcoDI GTCTC 3 cut(s) 452, 692, 899
BcuI ACTAGT 1 cut(s) 889
BfaI CTAG 4 cut(s) 11, 366, 890, 1237
BfmI CTRYAG 2 cut(s) 202, 1065
BglII AGATCT 1 cut(s) 962
BisI GCNGC 5 cut(s) 156, 859, 1065, 1074, 1130
BlnI CCTAGG 1 cut(s) 365
BlpI GCTNAGC 2 cut(s) 252, 942
BlsI GCNGC 5 cut(s) 157, 860, 1066, 1075, 1131
BmcAI AGTACT 1 cut(s) 1140
Bme1390I CCNGG 3 cut(s) 120, 1000, 1001
Bme18I GGWCC 2 cut(s) 547, 658
BmeT110I CYCGRG 1 cut(s) 999
BmgT120I GGNCC 2 cut(s) 547, 658
BmiI GGNNCC 7 cut(s) 62, 83, 117, 549, 660, 734, 997
BmrFI CCNGG 3 cut(s) 120, 1000, 1001
BmsI GCATC 2 cut(s) 265, 587
BmtI GCTAGC 1 cut(s) 14
BpiI GAAGAC 2 cut(s) 321, 662
BpmI CTGGAG 1 cut(s) 141
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 2 cut(s) 252, 942
BpuEI CTTGAG 4 cut(s) 23, 80, 276, 674
BpuMI CCSGG 2 cut(s) 1000, 1001
BsaI GGTCTC 1 cut(s) 692
BsaJI CCNNGG 4 cut(s) 240, 365, 622, 999
BsaXI ACNNNNNCTCC 2 cut(s) 922, 952
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 793
Bse1I ACTGG 2 cut(s) 304, 481
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 2 cut(s) 730, 1207
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 4 cut(s) 240, 365, 622, 999
BseGI GGATG 3 cut(s) 448, 523, 844
BseLI CCNNNNNNNGG 2 cut(s) 57, 793
BseMI GCAATG 2 cut(s) 730, 1207
BseMII CTCAG 4 cut(s) 179, 243, 429, 933
BseNI ACTGG 2 cut(s) 304, 481
BsePI GCGCGC 1 cut(s) 283
BseRI GAGGAG 3 cut(s) 401, 440, 554
BseXI GCAGC 5 cut(s) 142, 870, 1051, 1060, 1141
BseYI CCCAGC 1 cut(s) 110
BsgI GTGCAG 2 cut(s) 141, 1148
Bsh1236I CGCG 1 cut(s) 285
BshFI GGCC 4 cut(s) 334, 911, 1055, 1172
BshNI GGYRCC 2 cut(s) 81, 732
BsiHKAI GWGCWC 3 cut(s) 39, 214, 948
BsiHKCI CYCGRG 1 cut(s) 999
BsiSI CCGG 1 cut(s) 1000
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 2 cut(s) 57, 793
BsmAI GTCTC 3 cut(s) 452, 692, 899
BsmBI CGTCTC 1 cut(s) 899
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 4 cut(s) 334, 911, 1055, 1172
Bso31I GGTCTC 1 cut(s) 692
BsoBI CYCGRG 1 cut(s) 999
Bsp1286I GDGCHC 3 cut(s) 39, 214, 948
Bsp143I GATC 4 cut(s) 962, 967, 1157, 1206
Bsp1720I GCTNAGC 2 cut(s) 252, 942
Bsp19I CCATGG 1 cut(s) 622
BspACI CCGC 1 cut(s) 85
BspANI GGCC 4 cut(s) 334, 911, 1055, 1172
BspCNI CTCAG 4 cut(s) 178, 244, 430, 934
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 7 cut(s) 62, 83, 117, 549, 660, 734, 997
BspMAI CTGCAG 1 cut(s) 1069
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 1 cut(s) 1201
BspT107I GGYRCC 2 cut(s) 81, 732
BspTNI GGTCTC 1 cut(s) 692
BsrDI GCAATG 2 cut(s) 730, 1207
BsrI ACTGG 2 cut(s) 304, 481
BssECI CCNNGG 4 cut(s) 240, 365, 622, 999
BssHII GCGCGC 1 cut(s) 283
BssMI GATC 4 cut(s) 962, 967, 1157, 1206
BssT1I CCWWGG 3 cut(s) 240, 365, 622
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 749, 917, 1105
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstC8I GCNNGC 6 cut(s) 12, 16, 285, 498, 880, 884
BstDEI CTNAG 4 cut(s) 165, 252, 438, 942
BstDSI CCRYGG 1 cut(s) 622
BstF5I GGATG 3 cut(s) 448, 523, 844
BstFNI CGCG 1 cut(s) 285
BstHHI GCGC 5 cut(s) 102, 285, 287, 1014, 1179
BstKTI GATC 4 cut(s) 965, 970, 1160, 1209
BstMAI GTCTC 3 cut(s) 452, 692, 899
BstMBI GATC 4 cut(s) 962, 967, 1157, 1206
BstMWI GCNNNNNNNGC 9 cut(s) 20, 152, 161, 356, 567, 1020, 1061, 1070, 1073
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 2 cut(s) 500, 613
BstSCI CCNGG 3 cut(s) 118, 998, 999
BstSFI CTRYAG 2 cut(s) 202, 1065
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 5 cut(s) 142, 870, 1051, 1060, 1141
BstV2I GAAGAC 2 cut(s) 321, 662
BstX2I RGATCY 1 cut(s) 962
BstYI RGATCY 1 cut(s) 962
Bsu36I CCTNAGG 1 cut(s) 438
BsuRI GGCC 4 cut(s) 334, 911, 1055, 1172
BtgI CCRYGG 1 cut(s) 622
BtsCI GGATG 3 cut(s) 448, 523, 844
BtsI GCAGTG 2 cut(s) 787, 1074
BtsIMutI CAGTG 3 cut(s) 787, 922, 1074
Cac8I GCNNGC 6 cut(s) 12, 16, 285, 498, 880, 884
CaiI CAGNNNCTG 1 cut(s) 1073
CfoI GCGC 5 cut(s) 102, 285, 287, 1014, 1179
Cfr13I GGNCC 2 cut(s) 547, 658
Cfr9I CCCGGG 1 cut(s) 999
Csp6I GTAC 4 cut(s) 718, 797, 1081, 1139
CviQI GTAC 4 cut(s) 718, 797, 1081, 1139
DdeI CTNAG 4 cut(s) 165, 252, 438, 942
DpnI GATC 4 cut(s) 964, 969, 1159, 1208
DpnII GATC 4 cut(s) 962, 967, 1157, 1206
DraI TTTAAA 1 cut(s) 483
EaeI YGGCCR 2 cut(s) 909, 1053
Ecl136II GAGCTC 2 cut(s) 37, 946
Eco130I CCWWGG 3 cut(s) 240, 365, 622
Eco24I GRGCYC 2 cut(s) 39, 948
Eco31I GGTCTC 1 cut(s) 692
Eco32I GATATC 1 cut(s) 978
Eco47I GGWCC 2 cut(s) 547, 658
Eco53kI GAGCTC 2 cut(s) 37, 946
Eco57I CTGAAG 1 cut(s) 1163
Eco81I CCTNAGG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 999
EcoICRI GAGCTC 2 cut(s) 37, 946
EcoO109I RGGNCCY 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 118
EcoRV GATATC 1 cut(s) 978
EcoT14I CCWWGG 3 cut(s) 240, 365, 622
EcoT22I ATGCAT 1 cut(s) 602
EcoT38I GRGCYC 2 cut(s) 39, 948
ErhI CCWWGG 3 cut(s) 240, 365, 622
Esp3I CGTCTC 1 cut(s) 899
FalI AAGNNNNNCTT 4 cut(s) 144, 176, 657, 689
FaqI GGGAC 1 cut(s) 310
FauNDI CATATG 2 cut(s) 771, 1050
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 5 cut(s) 156, 859, 1065, 1074, 1130
FokI GGATG 3 cut(s) 455, 530, 851
FriOI GRGCYC 2 cut(s) 39, 948
Fsp4HI GCNGC 5 cut(s) 156, 859, 1065, 1074, 1130
FspBI CTAG 4 cut(s) 11, 366, 890, 1237
FspI TGCGCA 1 cut(s) 1178
GlaI GCGC 5 cut(s) 101, 284, 286, 1013, 1178
GluI GCNGC 5 cut(s) 156, 859, 1065, 1074, 1130
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 4 cut(s) 334, 911, 1055, 1172
HapII CCGG 1 cut(s) 1000
HhaI GCGC 5 cut(s) 102, 285, 287, 1014, 1179
Hin6I GCGC 5 cut(s) 100, 283, 285, 1012, 1177
HinP1I GCGC 5 cut(s) 100, 283, 285, 1012, 1177
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 5 cut(s) 343, 391, 604, 627, 1164
HpaII CCGG 1 cut(s) 1000
HphI GGTGA 4 cut(s) 314, 827, 914, 1032
Hpy166II GTNNAC 2 cut(s) 433, 799
Hpy188I TCNGA 4 cut(s) 76, 342, 967, 1211
Hpy188III TCNNGA 5 cut(s) 167, 267, 395, 638, 1161
Hpy8I GTNNAC 2 cut(s) 433, 799
HpyAV CCTTC 2 cut(s) 649, 1138
HpyCH4III ACNGT 3 cut(s) 749, 917, 1105
HpyF10VI GCNNNNNNNGC 9 cut(s) 20, 152, 161, 356, 567, 1020, 1061, 1070, 1073
HpyF3I CTNAG 4 cut(s) 165, 252, 438, 942
HspAI GCGC 5 cut(s) 100, 283, 285, 1012, 1177
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 4 cut(s) 962, 967, 1157, 1206
LmnI GCTCC 2 cut(s) 66, 364
Lsp1109I GCAGC 5 cut(s) 142, 870, 1051, 1060, 1141
LweI GCATC 2 cut(s) 265, 587
MaeI CTAG 4 cut(s) 11, 366, 890, 1237
MaeIII GTNAC 4 cut(s) 26, 124, 272, 392
MalI GATC 4 cut(s) 964, 969, 1159, 1208
MboI GATC 4 cut(s) 962, 967, 1157, 1206
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 3 cut(s) 39, 214, 948
MlsI TGGCCA 1 cut(s) 1055
MluCI AATT 2 cut(s) 378, 1004
MluNI TGGCCA 1 cut(s) 1055
MlyI GAGTC 3 cut(s) 352, 400, 1173
MmeI TCCRAC 2 cut(s) 525, 816
Mox20I TGGCCA 1 cut(s) 1055
Mph1103I ATGCAT 1 cut(s) 602
MscI TGGCCA 1 cut(s) 1055
MseI TTAA 3 cut(s) 482, 696, 744
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 1055
MspA1I CMGCKG 3 cut(s) 207, 1064, 1132
MspI CCGG 1 cut(s) 1000
MspR9I CCNGG 3 cut(s) 120, 1000, 1001
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 9 cut(s) 20, 152, 161, 356, 567, 1020, 1061, 1070, 1073
NciI CCSGG 2 cut(s) 1000, 1001
NcoI CCATGG 1 cut(s) 622
NdeI CATATG 2 cut(s) 771, 1050
NdeII GATC 4 cut(s) 962, 967, 1157, 1206
NheI GCTAGC 1 cut(s) 10
NlaIV GGNNCC 7 cut(s) 62, 83, 117, 549, 660, 734, 997
NmuCI GTSAC 3 cut(s) 124, 272, 392
NsbI TGCGCA 1 cut(s) 1178
NsiI ATGCAT 1 cut(s) 602
NspI RCATGY 2 cut(s) 500, 613
OliI CACNNNNGTG 1 cut(s) 399
PaeI GCATGC 1 cut(s) 500
PauI GCGCGC 1 cut(s) 283
PfeI GAWTC 2 cut(s) 604, 627
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 5 cut(s) 157, 860, 1066, 1075, 1131
PleI GAGTC 3 cut(s) 351, 399, 1172
PpsI GAGTC 3 cut(s) 351, 399, 1172
PpuMI RGGWCCY 1 cut(s) 658
Psp124BI GAGCTC 2 cut(s) 39, 948
Psp5II RGGWCCY 1 cut(s) 658
Psp6I CCWGG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 110
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 7 cut(s) 62, 83, 117, 549, 660, 734, 997
PspPI GGNCC 2 cut(s) 547, 658
PspPPI RGGWCCY 1 cut(s) 658
PsrI GAACNNNNNNTAC 2 cut(s) 99, 131
PstI CTGCAG 1 cut(s) 1069
PstNI CAGNNNCTG 1 cut(s) 1073
PsuI RGATCY 1 cut(s) 962
PteI GCGCGC 1 cut(s) 283
PvuII CAGCTG 3 cut(s) 207, 1064, 1132
RsaI GTAC 4 cut(s) 719, 798, 1082, 1140
RsaNI GTAC 4 cut(s) 718, 797, 1081, 1139
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 2 cut(s) 39, 948
SaqAI TTAA 3 cut(s) 482, 696, 744
SatI GCNGC 5 cut(s) 156, 859, 1065, 1074, 1130
Sau3AI GATC 4 cut(s) 962, 967, 1157, 1206
Sau96I GGNCC 2 cut(s) 547, 658
ScaI AGTACT 1 cut(s) 1140
SchI GAGTC 3 cut(s) 352, 400, 1173
ScrFI CCNGG 3 cut(s) 120, 1000, 1001
SduI GDGCHC 3 cut(s) 39, 214, 948
SfaNI GCATC 2 cut(s) 265, 587
SfcI CTRYAG 2 cut(s) 202, 1065
SinI GGWCC 2 cut(s) 547, 658
SmaI CCCGGG 1 cut(s) 1001
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 4 cut(s) 38, 95, 291, 689
SmoI CTYRAG 4 cut(s) 38, 95, 291, 689
SpeI ACTAGT 1 cut(s) 889
SphI GCATGC 1 cut(s) 500
Sse9I AATT 2 cut(s) 378, 1004
SsiI CCGC 1 cut(s) 85
SspMI CTAG 4 cut(s) 11, 366, 890, 1237
SstI GAGCTC 2 cut(s) 39, 948
StyD4I CCNGG 3 cut(s) 118, 998, 999
StyI CCWWGG 3 cut(s) 240, 365, 622
TaaI ACNGT 3 cut(s) 749, 917, 1105
TaqI TCGA 3 cut(s) 870, 921, 1167
TasI AATT 2 cut(s) 378, 1004
TatI WGTACW 2 cut(s) 717, 1138
TfiI GAWTC 2 cut(s) 604, 627
Tru1I TTAA 3 cut(s) 482, 696, 744
Tru9I TTAA 3 cut(s) 482, 696, 744
TscAI CASTG 3 cut(s) 794, 922, 1074
TseFI GTSAC 3 cut(s) 124, 272, 392
TseI GCWGC 5 cut(s) 155, 858, 1064, 1073, 1129
Tsp45I GTSAC 3 cut(s) 124, 272, 392
TspDTI ATGAA 7 cut(s) 201, 321, 534, 622, 697, 834, 1208
TspMI CCCGGG 1 cut(s) 999
TspRI CASTG 3 cut(s) 794, 922, 1074
VpaK11BI GGWCC 2 cut(s) 547, 658
XapI RAATTY 1 cut(s) 1004
XceI RCATGY 2 cut(s) 500, 613
XmaI CCCGGG 1 cut(s) 999
XmaJI CCTAGG 1 cut(s) 365
XspI CTAG 4 cut(s) 11, 366, 890, 1237
ZrmI AGTACT 1 cut(s) 1140
Zsp2I ATGCAT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.