MD06G1198400.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004492
Physical Location & Seq
Reverse (-)
33238673 .. 33240419
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1198400.v1.1.491

Sequence Viewer

Length: 684 bp
ATGCTGATGTCTCATCATCTCTCATCTCTTAACTCTATTTTTCTCCAACAAATACACACATTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTGAGGCATTGTTCTTCTTCTTCTTCTTTCTTCTTGCAATCTGGTGTTTTAGTGAAGAGTTGGAAAATGGAGAAAACTCTGACAAGAGTTGGGAGCTTTTGGGTTTCCAAGAAAGCTAAGGAAGAGATCTCCAACATCACTCAAGATCTCTCTTCATTATCAAACACTGTTGAGGAGAAAGCAAAACGGATATTCAACAAGCTCAAAGGTAAGCAACAAAAACCGTTGCCTGATCTTCTCCGAGAGTACTACCTTCCACCGGGCCTGTTTCCCCGGAACATAACATGTTACGAATTTGACGAATCAAAGGGCAAGCTCATTGTTTACTTGCCTTCTCCCTGTGAGGTGAGCTTCAAGGACTCATCTGTTGTACGATACAGCATTCGCATCAAAGGAACATTGTCGAGGGGAAAGCTTAGTGGAATCGAAGGAATGAAGACAAGGGTTCTGGTGTGGGTGAAAGTCACTAGCGTTTCTGTCGAGGGCTATAAGTCTGAAAAAGTATGGTTCACCGCTGGTATGAAGAAATCACGACCCAAAGATGCTTATGAAATGCCTCGCGATGCCATTATAGTCGAAGAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

228

Amino Acids

26.29

Weight (kDa)

9.76

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 110 - 216 3.4e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 657
AciI CCGC 1 cut(s) 609
AcsI RAATTY 2 cut(s) 389, 677
AfaI GTAC 2 cut(s) 344, 468
AfiI CCNNNNNNNGG 1 cut(s) 355
AflIII ACRYGT 1 cut(s) 380
AgsI TTSAA 2 cut(s) 292, 451
AluBI AGCT 6 cut(s) 192, 212, 298, 412, 447, 511
AluI AGCT 6 cut(s) 192, 212, 298, 412, 447, 511
Alw26I GTCTC 1 cut(s) 15
AoxI GGCC 1 cut(s) 358
ApoI RAATTY 2 cut(s) 389, 677
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 2 cut(s) 357, 370
AsuHPI GGTGA 3 cut(s) 454, 565, 598
BbsI GAAGAC 1 cut(s) 539
BbvCI CCTCAGC 1 cut(s) 98
BcnI CCSGG 2 cut(s) 357, 370
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 1 cut(s) 564
BglII AGATCT 2 cut(s) 222, 241
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 2 cut(s) 357, 370
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 357, 370
BmsI GCATC 3 cut(s) 492, 628, 649
BpiI GAAGAC 1 cut(s) 539
Bpu10I CCTNAGC 2 cut(s) 98, 213
BpuEI CTTGAG 1 cut(s) 222
BpuMI CCSGG 2 cut(s) 357, 370
BsaJI CCNNGG 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 355
BseDI CCNNGG 1 cut(s) 368
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 657
BshFI GGCC 1 cut(s) 360
BsiSI CCGG 2 cut(s) 356, 370
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 15
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 1 cut(s) 360
Bsp143I GATC 3 cut(s) 222, 241, 328
Bsp68I TCGCGA 1 cut(s) 657
BspACI CCGC 1 cut(s) 609
BspANI GGCC 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 1 cut(s) 657
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 3 cut(s) 222, 241, 328
Bst4CI ACNGT 2 cut(s) 265, 321
Bst6I CTCTTC 3 cut(s) 146, 213, 253
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 3 cut(s) 98, 213, 512
BstFNI CGCG 1 cut(s) 657
BstKTI GATC 3 cut(s) 225, 244, 331
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 3 cut(s) 222, 241, 328
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 2 cut(s) 355, 368
BstUI CGCG 1 cut(s) 657
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 2 cut(s) 222, 241
BstYI RGATCY 2 cut(s) 222, 241
BsuRI GGCC 1 cut(s) 360
BtgZI GCGATG 1 cut(s) 672
BtsIMutI CAGTG 1 cut(s) 261
BtuMI TCGCGA 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 2 cut(s) 343, 467
CviAII CATG 1 cut(s) 381
CviJI RGCY 9 cut(s) 97, 192, 212, 298, 360, 412, 447, 511, 582
CviKI_1 RGCY 9 cut(s) 97, 192, 212, 298, 360, 412, 447, 511, 582
CviQI GTAC 2 cut(s) 343, 467
DdeI CTNAG 3 cut(s) 98, 213, 512
DpnI GATC 3 cut(s) 224, 243, 330
DpnII GATC 3 cut(s) 222, 241, 328
Eam1104I CTCTTC 3 cut(s) 146, 213, 253
EarI CTCTTC 3 cut(s) 146, 213, 253
EcoRI GAATTC 1 cut(s) 677
FaeI CATG 1 cut(s) 384
FaiI YATR 7 cut(s) 377, 382, 585, 601, 617, 645, 668
FatI CATG 1 cut(s) 380
FspBI CTAG 1 cut(s) 564
HaeIII GGCC 1 cut(s) 360
HapII CCGG 2 cut(s) 356, 370
Hin1II CATG 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 3 cut(s) 398, 455, 519
HpaII CCGG 2 cut(s) 356, 370
HphI GGTGA 3 cut(s) 454, 565, 598
Hpy166II GTNNAC 2 cut(s) 421, 606
Hpy188I TCNGA 3 cut(s) 177, 338, 592
Hpy188III TCNNGA 3 cut(s) 239, 627, 656
Hpy8I GTNNAC 2 cut(s) 421, 606
HpyAV CCTTC 3 cut(s) 359, 438, 518
HpyCH4III ACNGT 2 cut(s) 265, 321
HpyCH4V TGCA 1 cut(s) 133
HpyF3I CTNAG 3 cut(s) 98, 213, 512
Hsp92II CATG 1 cut(s) 384
Kzo9I GATC 3 cut(s) 222, 241, 328
LmnI GCTCC 1 cut(s) 189
LpnPI CCDG 8 cut(s) 123, 339, 369, 374, 383, 448, 530, 597
LweI GCATC 3 cut(s) 492, 628, 649
MaeI CTAG 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 383, 559
MalI GATC 3 cut(s) 224, 243, 330
MboI GATC 3 cut(s) 222, 241, 328
MflI RGATCY 2 cut(s) 222, 241
MluCI AATT 2 cut(s) 389, 677
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 3 cut(s) 70, 137, 252
MnlI CCTC 7 cut(s) 87, 93, 262, 433, 495, 571, 663
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 2 cut(s) 356, 370
MspR9I CCNGG 2 cut(s) 357, 370
Mva1269I GAATGC 1 cut(s) 477
MvnI CGCG 1 cut(s) 657
NciI CCSGG 2 cut(s) 357, 370
NdeII GATC 3 cut(s) 222, 241, 328
NlaIII CATG 1 cut(s) 384
NmuCI GTSAC 1 cut(s) 559
NruI TCGCGA 1 cut(s) 657
NspI RCATGY 1 cut(s) 384
PciI ACATGT 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 1 cut(s) 477
PfeI GAWTC 2 cut(s) 398, 519
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PscI ACATGT 1 cut(s) 380
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 2 cut(s) 222, 241
RruI TCGCGA 1 cut(s) 657
RsaI GTAC 2 cut(s) 344, 468
RsaNI GTAC 2 cut(s) 343, 467
SaqAI TTAA 1 cut(s) 30
Sau3AI GATC 3 cut(s) 222, 241, 328
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 2 cut(s) 357, 370
SetI ASST 9 cut(s) 194, 214, 300, 307, 351, 414, 444, 449, 513
SfaNI GCATC 3 cut(s) 492, 628, 649
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 2 cut(s) 389, 677
SsiI CCGC 1 cut(s) 609
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 355, 368
TaaI ACNGT 2 cut(s) 265, 321
TaqI TCGA 4 cut(s) 500, 522, 576, 672
TasI AATT 2 cut(s) 389, 677
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 2 cut(s) 398, 519
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 1 cut(s) 559
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 4 cut(s) 240, 545, 632, 660
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 268
XapI RAATTY 2 cut(s) 389, 677
XceI RCATGY 1 cut(s) 384
XspI CTAG 1 cut(s) 564
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.