FvH4_5g14440

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004492
Physical Location & Seq
Forward (+)
8172287 .. 8174837
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14440.t1

Sequence Viewer

Length: 528 bp
ATGGAGAACACTCTGACAAAAGTTATTGGGAGCTCTTGGATTGCTAAGAAAGCAAAGGAAGAGATCAACAACATCACAGATGATTTATCTTCAATCTCAAACGCTGTGGAGGGAAAGGCGAAATGGGTGTTCAACAAGTTTAAAGGCAAGCCACAAAGAACGTTGCCAGAGCTGCTCCGATTGTACAACCTTCCTCCGGGCCTATTTCCCCGGAACATAAAATGCTATGAGTTCGACGAATCAAAGGGGAAGCTCATTGTGTACATGAACTCTGCCTGCGAGGTGAGCTTCAAGGACTCGTCTGTTGTAAGGTACGGGGCTAGGGTCAAGGCATCATTGACAAGGGGAAAGCTGGATGCAATAGAAGGAATGAAGACAAAGGTTGTTCTGGTTTATGTCAAAGTCACTAGTTTAGCTGTAGAAGGCTACAAGTCTGATAAAGTTTGGTTCACTGCTGGCTTGAAGAGATCAAGACCAAAAGATGCATATGAAATGCCTCGTGATGCCTCCGGTGTTGAAGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

176

Amino Acids

19.7

Weight (kDa)

9.54

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 56 - 164 1.3e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 161
AcsI RAATTY 1 cut(s) 521
AfaI GTAC 3 cut(s) 185, 263, 314
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 5 cut(s) 93, 133, 292, 463, 518
AhlI ACTAGT 1 cut(s) 407
AluBI AGCT 6 cut(s) 33, 172, 253, 288, 352, 416
AluI AGCT 6 cut(s) 33, 172, 253, 288, 352, 416
Alw21I GWGCWC 1 cut(s) 35
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 172
ApoI RAATTY 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 2 cut(s) 198, 211
AsuHPI GGTGA 1 cut(s) 295
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 159
BcnI CCSGG 2 cut(s) 198, 211
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 2 cut(s) 321, 408
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme1390I CCNGG 2 cut(s) 198, 211
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 2 cut(s) 198, 211
BmsI GCATC 4 cut(s) 341, 346, 472, 493
BpiI GAAGAC 1 cut(s) 380
BpuMI CCSGG 2 cut(s) 198, 211
BsaJI CCNNGG 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 509
Bsc4I CCNNNNNNNGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 3 cut(s) 197, 211, 510
BslI CCNNNNNNNGG 1 cut(s) 196
BsnI GGCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 35
Bsp1407I TGTACA 2 cut(s) 183, 261
Bsp143I GATC 2 cut(s) 63, 467
BspANI GGCC 1 cut(s) 201
BsrGI TGTACA 2 cut(s) 183, 261
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 2 cut(s) 63, 467
BssSI CACGAG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 498
Bst6I CTCTTC 2 cut(s) 54, 458
BstAUI TGTACA 2 cut(s) 183, 261
BstC8I GCNNGC 3 cut(s) 149, 277, 457
BstDEI CTNAG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 2 cut(s) 66, 470
BstMBI GATC 2 cut(s) 63, 467
BstMWI GCNNNNNNNGC 3 cut(s) 50, 172, 285
BstSCI CCNGG 2 cut(s) 196, 209
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 380
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 361
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 3 cut(s) 149, 277, 457
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 3 cut(s) 184, 262, 313
CspCI CAANNNNNGTGG 2 cut(s) 87, 122
CviAII CATG 1 cut(s) 265
CviQI GTAC 3 cut(s) 184, 262, 313
DdeI CTNAG 1 cut(s) 45
DpnI GATC 2 cut(s) 65, 469
DpnII GATC 2 cut(s) 63, 467
DraI TTTAAA 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 54, 458
EarI CTCTTC 2 cut(s) 54, 458
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco53kI GAGCTC 1 cut(s) 33
EcoICRI GAGCTC 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 1 cut(s) 268
FaiI YATR 6 cut(s) 218, 228, 266, 396, 487, 489
FatI CATG 1 cut(s) 264
FauNDI CATATG 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 173
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 1 cut(s) 173
FspBI CTAG 2 cut(s) 321, 408
GluI GCNGC 1 cut(s) 173
HaeIII GGCC 1 cut(s) 201
HapII CCGG 3 cut(s) 197, 211, 510
Hin1II CATG 1 cut(s) 268
HinfI GANTC 2 cut(s) 239, 296
HpaII CCGG 3 cut(s) 197, 211, 510
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 262, 450
Hpy188I TCNGA 3 cut(s) 15, 179, 436
Hpy188III TCNNGA 2 cut(s) 471, 500
Hpy8I GTNNAC 2 cut(s) 262, 450
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 200, 359, 416
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 2 cut(s) 359, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 172, 285
HpyF3I CTNAG 1 cut(s) 45
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 1 cut(s) 268
Kzo9I GATC 2 cut(s) 63, 467
LmnI GCTCC 2 cut(s) 30, 180
LpnPI CCDG 8 cut(s) 180, 210, 224, 289, 338, 374, 441, 523
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 4 cut(s) 341, 346, 472, 493
MaeI CTAG 2 cut(s) 321, 408
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 1 cut(s) 403
MalI GATC 2 cut(s) 65, 469
MboI GATC 2 cut(s) 63, 467
MboII GAAGA 4 cut(s) 71, 81, 385, 475
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 1 cut(s) 521
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 5 cut(s) 103, 204, 274, 507, 517
Mph1103I ATGCAT 1 cut(s) 487
MseI TTAA 2 cut(s) 141, 526
MspI CCGG 3 cut(s) 197, 211, 510
MspR9I CCNGG 2 cut(s) 198, 211
MwoI GCNNNNNNNGC 3 cut(s) 50, 172, 285
NciI CCSGG 2 cut(s) 198, 211
NdeI CATATG 1 cut(s) 487
NdeII GATC 2 cut(s) 63, 467
NlaIII CATG 1 cut(s) 268
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 487
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 1 cut(s) 174
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp124BI GAGCTC 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 161
PspPI GGNCC 1 cut(s) 199
RsaI GTAC 3 cut(s) 185, 263, 314
RsaNI GTAC 3 cut(s) 184, 262, 313
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 2 cut(s) 141, 526
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 2 cut(s) 63, 467
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 198, 211
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 4 cut(s) 341, 346, 472, 493
SfcI CTRYAG 1 cut(s) 417
SpeI ACTAGT 1 cut(s) 407
Sse9I AATT 1 cut(s) 521
SspMI CTAG 2 cut(s) 321, 408
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 196, 209
TaiI ACGT 1 cut(s) 164
TaqI TCGA 1 cut(s) 234
TasI AATT 1 cut(s) 521
TatI WGTACW 2 cut(s) 183, 261
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 141, 526
Tru9I TTAA 2 cut(s) 141, 526
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 3 cut(s) 281, 386, 504
TspRI CASTG 1 cut(s) 457
XapI RAATTY 1 cut(s) 521
XspI CTAG 2 cut(s) 321, 408
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.