Rh7DG048500

At5g01610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004492
Physical Location & Seq
Reverse (-)
3739350 .. 3741929
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG048500.1

Sequence Viewer

Length: 519 bp
ATGAATATACACTGTAGGCAGAATGGTTCCTTAATAACTAATGTGTGTTTCTGTTGGTTTTCTAAATTAAAACAATTTCAGTTACTCTCAAACGCTGTGGAGGGAAAGGCAAAATGGGTTTTCAACAAGCTTAAAGGCAAGCCACTGAGAACTTTGCCGGATCTTCTCCGGCTGTACAATCTTCCTCCAGGCCTGTTTCCCCGAAACATAAAATGCTACGAGTTCGACGAATCAAAGGGGAAGCTCATTGTGTACTTGACCTCTGCCTGTGAGGTGAGCTTCAAGGACTCATCTGTGGTAAGGTACGCAGTTCGAGTCAAAGCAACATTGACAAGGGGAAAGCTGGATGTAATAGAAGGAATGAAGACAAAGGTTATGCTGGTTTATATCAAAGTCACTAGTGTAGCTGTAGAAGGCTACAAGTCTGATAAAGTTTGGTTCACTGCTGGGTTGAAGAGATCAAGACCCAAAGATGCCTACGAAATGCCTCGTGATGCCTTCAGTGTTGATGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

172

Amino Acids

19.72

Weight (kDa)

9.69

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 54 - 161 7.9e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 168
AcsI RAATTY 1 cut(s) 512
AcuI CTGAAG 1 cut(s) 484
AfaI GTAC 3 cut(s) 176, 254, 305
AgsI TTSAA 3 cut(s) 124, 283, 454
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 5 cut(s) 130, 244, 279, 343, 407
AluI AGCT 5 cut(s) 130, 244, 279, 343, 407
AlwI GGATC 1 cut(s) 168
AoxI GGCC 1 cut(s) 190
ApoI RAATTY 1 cut(s) 512
AsuHPI GGTGA 1 cut(s) 286
BauI CACGAG 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 371
BciT130I CCWGG 1 cut(s) 189
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 2 cut(s) 13, 408
Bme1390I CCNGG 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 463, 484
BpiI GAAGAC 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 352
BseMII CTCAG 1 cut(s) 137
BseYI CCCAGC 1 cut(s) 446
BshFI GGCC 1 cut(s) 192
BsiSI CCGG 2 cut(s) 158, 169
BsnI GGCC 1 cut(s) 192
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 2 cut(s) 160, 458
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 28
BspPI GGATC 1 cut(s) 168
BsrGI TGTACA 1 cut(s) 174
BssMI GATC 2 cut(s) 160, 458
BssSI CACGAG 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 146
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 2 cut(s) 163, 461
BstMBI GATC 2 cut(s) 160, 458
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 2 cut(s) 13, 408
BstV2I GAAGAC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BsuRI GGCC 1 cut(s) 192
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 4 cut(s) 10, 143, 441, 508
Cac8I GCNNGC 1 cut(s) 140
Csp6I GTAC 3 cut(s) 175, 253, 304
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviJI RGCY 9 cut(s) 130, 142, 172, 192, 244, 279, 343, 407, 417
CviKI_1 RGCY 9 cut(s) 130, 142, 172, 192, 244, 279, 343, 407, 417
CviQI GTAC 3 cut(s) 175, 253, 304
DdeI CTNAG 1 cut(s) 146
DpnI GATC 2 cut(s) 162, 460
DpnII GATC 2 cut(s) 160, 458
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
Eco147I AGGCCT 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 187
FaiI YATR 4 cut(s) 8, 209, 377, 387
FokI GGATG 1 cut(s) 359
FspBI CTAG 1 cut(s) 399
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 1 cut(s) 192
HapII CCGG 2 cut(s) 158, 169
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 230, 287, 315
HpaII CCGG 2 cut(s) 158, 169
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 2 cut(s) 253, 441
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 2 cut(s) 462, 491
Hpy8I GTNNAC 2 cut(s) 253, 441
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 3 cut(s) 350, 407, 508
HpyCH4III ACNGT 1 cut(s) 14
HpyF3I CTNAG 1 cut(s) 146
Kzo9I GATC 2 cut(s) 160, 458
LpnPI CCDG 9 cut(s) 171, 174, 182, 201, 206, 280, 329, 365, 432
LweI GCATC 2 cut(s) 463, 484
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 2 cut(s) 81, 394
MalI GATC 2 cut(s) 162, 460
MboI GATC 2 cut(s) 160, 458
MboII GAAGA 4 cut(s) 155, 173, 376, 466
MflI RGATCY 1 cut(s) 160
MluCI AATT 3 cut(s) 65, 74, 512
MlyI GAGTC 2 cut(s) 281, 324
MnlI CCTC 5 cut(s) 94, 195, 265, 271, 498
MseI TTAA 4 cut(s) 32, 68, 132, 517
MspI CCGG 2 cut(s) 158, 169
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
NdeII GATC 2 cut(s) 160, 458
NlaIV GGNNCC 1 cut(s) 28
NmuCI GTSAC 1 cut(s) 394
PceI AGGCCT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 1 cut(s) 230
PleI GAGTC 2 cut(s) 281, 323
PpsI GAGTC 2 cut(s) 281, 323
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 28
PsuI RGATCY 1 cut(s) 160
RsaI GTAC 3 cut(s) 176, 254, 305
RsaNI GTAC 3 cut(s) 175, 253, 304
SaqAI TTAA 4 cut(s) 32, 68, 132, 517
Sau3AI GATC 2 cut(s) 160, 458
SchI GAGTC 2 cut(s) 281, 324
ScrFI CCNGG 1 cut(s) 189
SetI ASST 9 cut(s) 132, 246, 263, 276, 281, 305, 345, 375, 409
SfaNI GCATC 2 cut(s) 463, 484
SfcI CTRYAG 2 cut(s) 13, 408
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 3 cut(s) 65, 74, 512
SseBI AGGCCT 1 cut(s) 192
SspMI CTAG 1 cut(s) 399
StuI AGGCCT 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 1 cut(s) 14
TaqI TCGA 2 cut(s) 225, 313
TasI AATT 3 cut(s) 65, 74, 512
TatI WGTACW 2 cut(s) 174, 252
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 4 cut(s) 32, 68, 132, 517
Tru9I TTAA 4 cut(s) 32, 68, 132, 517
TscAI CASTG 4 cut(s) 17, 150, 448, 508
TseFI GTSAC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 2 cut(s) 17, 377
TspRI CASTG 4 cut(s) 17, 150, 448, 508
XapI RAATTY 1 cut(s) 512
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.