Rh7BG049300

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004492
Physical Location & Seq
Reverse (-)
3230471 .. 3232737
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG049300.1

Sequence Viewer

Length: 528 bp
ATGGAGAACGCTCTAACAAAAGTTATTGGGAGCAATTGGATTGCCAAAAAAGCCAAGGAAGAGATCAACAGCATCACTGATGACTTCTCTTTACTCTCAAACGCTGTGGAGGGAAAGGCAAAATGGGTTTTCAACAAGCTTAAAGGCAAGCCACTGAGAACTTTGCCGGATCTTCTCCGGCTGTACAATCTTCCTCCAGGCCTGTTTCCCCGAAACATAAAATGCTACGAGTTCGACGAATCAAAGGGGAAGCTCATTGTGTACTTGACCTCTGCCTGTGAGGTGAGCTTCAAGGACTCATCTGTGGTAAGGTACGCAGTTCGAGTCAAAGCAACATTGACAAGGGGAAAGCTGGATGTAATAGAAGGAATGAAGACAAAGGTTATGCTGGTTTATATCAAAGTCACTAGTGTAGCTGTAGAAGGCTACAAGTCTGATAAAGTTTGGTTCACTGCTGGGTTGAAGAGATCAAGACCCAAAGATGCCTACGAAATGCCTCGTGATGCCTTCAGTGTTGATGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

175

Amino Acids

19.82

Weight (kDa)

9.54

Isoelectric Point (pI)

25.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 57 - 164 8.3e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 177
AcsI RAATTY 1 cut(s) 521
AcuI CTGAAG 1 cut(s) 493
AfaI GTAC 3 cut(s) 185, 263, 314
AgsI TTSAA 3 cut(s) 133, 292, 463
AhlI ACTAGT 1 cut(s) 407
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 5 cut(s) 139, 253, 288, 352, 416
AluI AGCT 5 cut(s) 139, 253, 288, 352, 416
AlwI GGATC 1 cut(s) 177
AoxI GGCC 1 cut(s) 199
ApoI RAATTY 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 295
BauI CACGAG 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 198
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 417
Bme1390I CCNGG 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 3 cut(s) 81, 472, 493
BpiI GAAGAC 1 cut(s) 380
BpmI CTGGAG 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 54
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 361
BseMII CTCAG 1 cut(s) 146
BseYI CCCAGC 1 cut(s) 455
BshFI GGCC 1 cut(s) 201
BsiSI CCGG 2 cut(s) 167, 178
BsnI GGCC 1 cut(s) 201
Bsp1407I TGTACA 1 cut(s) 183
Bsp143I GATC 3 cut(s) 63, 169, 467
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 147
BspPI GGATC 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 3 cut(s) 63, 169, 467
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 54
Bst2BI CACGAG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 198
Bst6I CTCTTC 2 cut(s) 54, 458
BstAUI TGTACA 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 3 cut(s) 66, 172, 470
BstMBI GATC 3 cut(s) 63, 169, 467
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 417
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 361
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 4 cut(s) 75, 152, 450, 517
Cac8I GCNNGC 1 cut(s) 149
Csp6I GTAC 3 cut(s) 184, 262, 313
CspCI CAANNNNNGTGG 2 cut(s) 87, 122
CviQI GTAC 3 cut(s) 184, 262, 313
DdeI CTNAG 1 cut(s) 155
DpnI GATC 3 cut(s) 65, 171, 469
DpnII GATC 3 cut(s) 63, 169, 467
Eam1104I CTCTTC 2 cut(s) 54, 458
EarI CTCTTC 2 cut(s) 54, 458
Eco130I CCWWGG 1 cut(s) 54
Eco147I AGGCCT 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaiI YATR 3 cut(s) 218, 386, 396
FokI GGATG 1 cut(s) 368
FspBI CTAG 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 1 cut(s) 201
HapII CCGG 2 cut(s) 167, 178
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 3 cut(s) 239, 296, 324
HpaII CCGG 2 cut(s) 167, 178
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 262, 450
Hpy188I TCNGA 1 cut(s) 436
Hpy188III TCNNGA 2 cut(s) 471, 500
Hpy8I GTNNAC 2 cut(s) 262, 450
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 359, 416, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 155
Kzo9I GATC 3 cut(s) 63, 169, 467
LmnI GCTCC 1 cut(s) 30
LpnPI CCDG 9 cut(s) 180, 183, 191, 210, 215, 289, 338, 374, 441
LweI GCATC 3 cut(s) 81, 472, 493
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 403
MalI GATC 3 cut(s) 65, 171, 469
MboI GATC 3 cut(s) 63, 169, 467
MboII GAAGA 5 cut(s) 71, 164, 182, 385, 475
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 1 cut(s) 169
MluCI AATT 2 cut(s) 34, 521
MlyI GAGTC 2 cut(s) 290, 333
MnlI CCTC 5 cut(s) 103, 204, 274, 280, 507
MseI TTAA 2 cut(s) 141, 526
MspI CCGG 2 cut(s) 167, 178
MspR9I CCNGG 1 cut(s) 198
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 3 cut(s) 63, 169, 467
NmuCI GTSAC 1 cut(s) 403
PceI AGGCCT 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 1 cut(s) 239
PleI GAGTC 2 cut(s) 290, 332
PpsI GAGTC 2 cut(s) 290, 332
Psp6I CCWGG 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 3 cut(s) 185, 263, 314
RsaNI GTAC 3 cut(s) 184, 262, 313
SaqAI TTAA 2 cut(s) 141, 526
Sau3AI GATC 3 cut(s) 63, 169, 467
SchI GAGTC 2 cut(s) 290, 333
ScrFI CCNGG 1 cut(s) 198
SetI ASST 9 cut(s) 141, 255, 272, 285, 290, 314, 354, 384, 418
SfaNI GCATC 3 cut(s) 81, 472, 493
SfcI CTRYAG 1 cut(s) 417
SpeI ACTAGT 1 cut(s) 407
Sse9I AATT 2 cut(s) 34, 521
SseBI AGGCCT 1 cut(s) 201
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 54
TaqI TCGA 2 cut(s) 234, 322
TasI AATT 2 cut(s) 34, 521
TatI WGTACW 2 cut(s) 183, 261
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 2 cut(s) 141, 526
Tru9I TTAA 2 cut(s) 141, 526
TscAI CASTG 4 cut(s) 82, 159, 457, 517
TseFI GTSAC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 386
TspRI CASTG 4 cut(s) 82, 159, 457, 517
XapI RAATTY 1 cut(s) 521
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.