AT2G03505

PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007655
Physical Location & Seq
Forward (+)
1063943 .. 1065471
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03505.4

Sequence Viewer

Length: 507 bp
ATGAGATTGCTTCTTAGTCTCTTGTTTCTCTTGGCTTTAACCACCTACTCAAGTGCAACTTATTGTCTATGTAGAGATGGTGTTGGAGAGAAAGATCTTCAAACATCGATAGATTATGCATGTGGAGTTTTAAAAGATTGTAATCCTATTCATGAGAAAGGTCCTTGTTATCAACCAAACACCATCAAGAGTCATTGTGATTGGGCGGTTAATACTTATTTCCAAAGATTTGGTCAAATCTCTGGAAGCTGTAATTTCTCAGGAACTGCTACTACCAGCCAAAATCTTCCTTCAACTGTGGTTACTGGTTGCTTATATCCTTCAAGTCCAGGAAGTGCAGGGACAACTCCCACGACAGGTACTCCTTCAGGAACACAGACGTTTCCGGGACCTCCTGCGTTTGGTCCGGCCGGAGATTTTGATCCTTCAGGCAATAATGGTGCTCCGAGTTTGTTCATTTCAATCGCTCTCTCACTTGGTTTCTCCGTCGTCATTGCGTTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

168

Amino Acids

17.55

Weight (kDa)

5.44

Isoelectric Point (pI)

31.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 21 - 91 4.6e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 206
AclWI GGATC 1 cut(s) 416
AcoI YGGCCR 1 cut(s) 408
AcuI CTGAAG 2 cut(s) 351, 411
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 2 cut(s) 356, 401
AgsI TTSAA 4 cut(s) 101, 294, 324, 462
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 1 cut(s) 249
AluI AGCT 1 cut(s) 249
Alw21I GWGCWC 1 cut(s) 445
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 1 cut(s) 416
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 408
ArsI GACNNNNNNTTYG 2 cut(s) 217, 249
AspS9I GGNCC 3 cut(s) 161, 389, 404
AsuC2I CCSGG 1 cut(s) 387
AvaII GGWCC 3 cut(s) 161, 389, 404
Bbv12I GWGCWC 1 cut(s) 445
BccI CCATC 2 cut(s) 71, 191
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 23
BglII AGATCT 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 330, 387
Bme18I GGWCC 3 cut(s) 161, 389, 404
BmgT120I GGNCC 3 cut(s) 161, 389, 404
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 330, 387
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 107
BsaBI GATNNNNATC 2 cut(s) 141, 420
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 401
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 492
Bse8I GATNNNNATC 2 cut(s) 141, 420
BseBI CCWGG 1 cut(s) 330
BseCI ATCGAT 1 cut(s) 107
BseJI GATNNNNATC 2 cut(s) 141, 420
BseLI CCNNNNNNNGG 2 cut(s) 356, 401
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 310
BseX3I CGGCCG 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 357
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 445
BsiSI CCGG 3 cut(s) 386, 407, 411
BslFI GGGAC 2 cut(s) 355, 402
BslI CCNNNNNNNGG 2 cut(s) 356, 401
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 2 cut(s) 355, 402
BsnI GGCC 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 445
Bsp143I GATC 2 cut(s) 94, 421
BspACI CCGC 1 cut(s) 206
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 1 cut(s) 272
BspDI ATCGAT 1 cut(s) 107
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 390
BspPI GGATC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 1 cut(s) 310
BssMI GATC 2 cut(s) 94, 421
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 298
BstDEI CTNAG 2 cut(s) 14, 259
BstKTI GATC 2 cut(s) 97, 424
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 2 cut(s) 94, 421
BstMCI CGRYCG 1 cut(s) 411
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 328, 385
BstX2I RGATCY 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 408
Bsu15I ATCGAT 1 cut(s) 107
BsuRI GGCC 1 cut(s) 410
BsuTUI ATCGAT 1 cut(s) 107
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 1 cut(s) 151
Cfr13I GGNCC 3 cut(s) 161, 389, 404
ClaI ATCGAT 1 cut(s) 107
Csp6I GTAC 1 cut(s) 360
CviAII CATG 2 cut(s) 120, 152
CviJI RGCY 4 cut(s) 35, 249, 279, 410
CviKI_1 RGCY 4 cut(s) 35, 249, 279, 410
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 2 cut(s) 14, 259
DpnI GATC 2 cut(s) 96, 423
DpnII GATC 2 cut(s) 94, 421
DraI TTTAAA 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 408
EagI CGGCCG 1 cut(s) 408
EclXI CGGCCG 1 cut(s) 408
Eco47I GGWCC 3 cut(s) 161, 389, 404
Eco52I CGGCCG 1 cut(s) 408
Eco57I CTGAAG 2 cut(s) 351, 411
EcoO109I RGGNCCY 2 cut(s) 161, 389
EcoRII CCWGG 1 cut(s) 328
EcoT22I ATGCAT 1 cut(s) 121
FaeI CATG 2 cut(s) 123, 155
FaiI YATR 6 cut(s) 70, 117, 121, 153, 316, 505
FalI AAGNNNNNCTT 2 cut(s) 43, 75
FaqI GGGAC 2 cut(s) 355, 402
FatI CATG 2 cut(s) 119, 151
HaeIII GGCC 1 cut(s) 410
HapII CCGG 3 cut(s) 386, 407, 411
Hin1II CATG 2 cut(s) 123, 155
HinfI GANTC 1 cut(s) 190
HpaII CCGG 3 cut(s) 386, 407, 411
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 5 cut(s) 152, 187, 243, 261, 369
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 4 cut(s) 300, 330, 375, 435
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 3 cut(s) 56, 119, 338
HpyF3I CTNAG 2 cut(s) 14, 259
HpySE526I ACGT 1 cut(s) 380
Hsp92II CATG 2 cut(s) 123, 155
Kzo9I GATC 2 cut(s) 94, 421
LmnI GCTCC 1 cut(s) 448
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 1 cut(s) 301
MalI GATC 2 cut(s) 96, 423
MboI GATC 2 cut(s) 94, 421
MboII GAAGA 2 cut(s) 89, 278
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 445
MluCI AATT 1 cut(s) 253
MlyI GAGTC 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 1 cut(s) 402
Mph1103I ATGCAT 1 cut(s) 121
MseI TTAA 3 cut(s) 38, 131, 210
MspI CCGG 3 cut(s) 386, 407, 411
MspR9I CCNGG 2 cut(s) 330, 387
MvaI CCWGG 1 cut(s) 330
NciI CCSGG 1 cut(s) 387
NdeII GATC 2 cut(s) 94, 421
NlaIII CATG 2 cut(s) 123, 155
NlaIV GGNNCC 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 123
PagI TCATGA 1 cut(s) 151
PfoI TCCNGGA 2 cut(s) 328, 385
PleI GAGTC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 198
PpuMI RGGWCCY 2 cut(s) 161, 389
Psp5II RGGWCCY 2 cut(s) 161, 389
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 3 cut(s) 161, 389, 404
PspPPI RGGWCCY 2 cut(s) 161, 389
PsrI GAACNNNNNNTAC 2 cut(s) 256, 288
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
SaqAI TTAA 3 cut(s) 38, 131, 210
Sau3AI GATC 2 cut(s) 94, 421
Sau96I GGNCC 3 cut(s) 161, 389, 404
SchI GAGTC 1 cut(s) 199
ScrFI CCNGG 2 cut(s) 330, 387
SduI GDGCHC 1 cut(s) 445
SetI ASST 6 cut(s) 47, 163, 251, 361, 383, 394
SinI GGWCC 3 cut(s) 161, 389, 404
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 1 cut(s) 253
SsiI CCGC 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 328, 385
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 1 cut(s) 383
TaqI TCGA 1 cut(s) 107
TasI AATT 1 cut(s) 253
Tru1I TTAA 3 cut(s) 38, 131, 210
Tru9I TTAA 3 cut(s) 38, 131, 210
TspDTI ATGAA 2 cut(s) 140, 445
TspGWI ACGGA 1 cut(s) 475
VpaK11BI GGWCC 3 cut(s) 161, 389, 404
XceI RCATGY 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.