MD16G1061700.v1.1

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007655
Physical Location & Seq
Reverse (-)
4400649 .. 4403468
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1061700.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGCTGCTTTAGTGTATATTGTGATTTTATTGGCCCTCACTGGCCATTCAAGTGCTATTTATTGCGTATGCAAAGATGGAATTGGTGATTCAGCACTTCAGAAGGCACTGGACTATGCCTGTGGAGCTGGAGCTGACTGCAGTCCAATCCTCCAAAACGGTGCGTGTTACCAGCCCAACACCATCAAAGATCACTGCAGCTATGCAGTCAACAGTTATTTCCAGAGGAAGGGTCAAACTGCACAGAGCTGTGATTTTTCTGGTGCTGCTTCTCAGAGCCAAACTGCTCCTAGTACCGCAAGTTCGACTTGTGTTTATCCTGCAAGTACCAGCCAAGCAGGAACTGGCTCTACCACTACAAACTCAACCACAGGCACAACTCCAAGCACAGGCACAACCCCAACCACCGGCACCGGCACCGGCACCGGCATCACAACTCCAGGCACTCCGGCATCAGTGTTCGGAATAAGCCCCGCCGGGAGTAGCACAGGAATCGGCAATACTGACGGCGCGGCTACCTTGGGAGGCGCCAACCAGTTCTTTTTCTCTCTAGCCTCAGCTCTGTGCTTCACAGTGTTGTTACTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

197

Amino Acids

19.39

Weight (kDa)

5.31

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 21 - 91 1.7e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 410, 416, 422, 527
AccII CGCG 1 cut(s) 512
AciI CCGC 3 cut(s) 297, 474, 512
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 83
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 2 cut(s) 295, 328
AfiI CCNNNNNNNGG 3 cut(s) 229, 389, 407
AgsI TTSAA 1 cut(s) 51
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 5 cut(s) 128, 134, 201, 249, 560
AluI AGCT 5 cut(s) 128, 134, 201, 249, 560
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 2 cut(s) 33, 43
ApeKI GCWGC 3 cut(s) 5, 198, 266
AspLEI GCGC 2 cut(s) 512, 530
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 478
AsuHPI GGTGA 1 cut(s) 98
BalI TGGCCA 1 cut(s) 45
BanI GGYRCC 4 cut(s) 410, 416, 422, 527
BbvCI CCTCAGC 1 cut(s) 556
BbvI GCAGC 2 cut(s) 210, 253
BccI CCATC 2 cut(s) 71, 191
BceAI ACGGC 1 cut(s) 523
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BciT130I CCWGG 1 cut(s) 441
BcnI CCSGG 1 cut(s) 478
BfaI CTAG 2 cut(s) 291, 551
BfmI CTRYAG 2 cut(s) 139, 196
BfoI RGCGCY 1 cut(s) 531
BisI GCNGC 4 cut(s) 6, 199, 267, 513
BlsI GCNGC 4 cut(s) 7, 200, 268, 514
Bme1390I CCNGG 2 cut(s) 441, 478
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 4 cut(s) 412, 418, 424, 529
BmrFI CCNGG 2 cut(s) 441, 478
BmsI GCATC 2 cut(s) 438, 461
BoxI GACNNNNGTC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 150, 423
Bpu10I CCTNAGC 1 cut(s) 556
BpuMI CCSGG 1 cut(s) 478
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 519
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 389, 407
Bse118I RCCGGY 4 cut(s) 407, 413, 419, 425
Bse1I ACTGG 4 cut(s) 46, 114, 349, 535
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 1 cut(s) 519
BseLI CCNNNNNNNGG 3 cut(s) 229, 389, 407
BseMII CTCAG 2 cut(s) 287, 570
BseNI ACTGG 4 cut(s) 46, 114, 349, 535
BseXI GCAGC 2 cut(s) 210, 253
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 2 cut(s) 35, 45
BshNI GGYRCC 4 cut(s) 410, 416, 422, 527
BsiSI CCGG 6 cut(s) 408, 414, 420, 426, 449, 477
BslI CCNNNNNNNGG 3 cut(s) 229, 389, 407
BsnI GGCC 2 cut(s) 35, 45
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 3 cut(s) 297, 474, 512
BspANI GGCC 2 cut(s) 35, 45
BspCNI CTCAG 2 cut(s) 286, 569
BspFNI CGCG 1 cut(s) 512
BspLI GGNNCC 4 cut(s) 412, 418, 424, 529
BspMAI CTGCAG 2 cut(s) 143, 200
BspT107I GGYRCC 4 cut(s) 410, 416, 422, 527
BsrFI RCCGGY 4 cut(s) 407, 413, 419, 425
BsrI ACTGG 4 cut(s) 46, 114, 349, 535
BssAI RCCGGY 4 cut(s) 407, 413, 419, 425
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 1 cut(s) 190
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 3 cut(s) 161, 215, 574
BstACI GRCGYC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 273, 556
BstFNI CGCG 1 cut(s) 512
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 2 cut(s) 512, 530
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 441
BstPAI GACNNNNGTC 1 cut(s) 141
BstSCI CCNGG 2 cut(s) 439, 476
BstSFI CTRYAG 2 cut(s) 139, 196
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 210, 253
BsuRI GGCC 2 cut(s) 35, 45
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 5 cut(s) 39, 107, 193, 462, 579
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 2 cut(s) 512, 530
Cfr10I RCCGGY 4 cut(s) 407, 413, 419, 425
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 294, 327
CviQI GTAC 2 cut(s) 294, 327
DdeI CTNAG 2 cut(s) 273, 556
DinI GGCGCC 1 cut(s) 529
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 519
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 519
FaiI YATR 4 cut(s) 18, 70, 117, 204
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FauI CCCGC 1 cut(s) 481
Fnu4HI GCNGC 4 cut(s) 6, 199, 267, 513
Fsp4HI GCNGC 4 cut(s) 6, 199, 267, 513
FspBI CTAG 2 cut(s) 291, 551
GlaI GCGC 2 cut(s) 511, 529
GluI GCNGC 4 cut(s) 6, 199, 267, 513
GsuI CTGGAG 2 cut(s) 150, 423
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 2 cut(s) 35, 45
HapII CCGG 6 cut(s) 408, 414, 420, 426, 449, 477
HhaI GCGC 2 cut(s) 512, 530
Hin1I GRCGYC 1 cut(s) 528
Hin6I GCGC 2 cut(s) 510, 528
HinP1I GCGC 2 cut(s) 510, 528
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 2 cut(s) 89, 492
HpaII CCGG 6 cut(s) 408, 414, 420, 426, 449, 477
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 102, 276, 464
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 2 cut(s) 97, 223
HpyCH4III ACNGT 3 cut(s) 161, 215, 574
HpyCH4V TGCA 6 cut(s) 72, 141, 198, 206, 242, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 273, 556
Hsp92I GRCGYC 1 cut(s) 528
HspAI GCGC 2 cut(s) 510, 528
KasI GGCGCC 1 cut(s) 527
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 3 cut(s) 125, 131, 292
Lsp1109I GCAGC 2 cut(s) 210, 253
LweI GCATC 2 cut(s) 438, 461
MaeI CTAG 2 cut(s) 291, 551
MaeIII GTNAC 2 cut(s) 167, 579
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 1 cut(s) 81
MluNI TGGCCA 1 cut(s) 45
Mly113I GGCGCC 1 cut(s) 528
MnlI CCTC 5 cut(s) 47, 161, 219, 518, 565
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 6 cut(s) 408, 414, 420, 426, 449, 477
MspR9I CCNGG 2 cut(s) 441, 478
MvaI CCWGG 1 cut(s) 441
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 125
NarI GGCGCC 1 cut(s) 528
NciI CCSGG 1 cut(s) 478
NdeII GATC 1 cut(s) 190
NlaIV GGNNCC 4 cut(s) 412, 418, 424, 529
PfeI GAWTC 2 cut(s) 89, 492
PkrI GCNGC 4 cut(s) 7, 200, 268, 514
PluTI GGCGCC 1 cut(s) 531
PshAI GACNNNNGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 4 cut(s) 412, 418, 424, 529
PspPI GGNCC 1 cut(s) 34
PsrI GAACNNNNNNTAC 4 cut(s) 286, 318, 334, 366
PstI CTGCAG 2 cut(s) 143, 200
PstNI CAGNNNCTG 1 cut(s) 344
RsaI GTAC 2 cut(s) 295, 328
RsaNI GTAC 2 cut(s) 294, 327
RseI CAYNNNNRTG 1 cut(s) 51
SatI GCNGC 4 cut(s) 6, 199, 267, 513
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 441, 478
SetI ASST 7 cut(s) 130, 136, 203, 251, 521, 562, 590
SfaNI GCATC 2 cut(s) 438, 461
SfcI CTRYAG 2 cut(s) 139, 196
SfoI GGCGCC 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 1 cut(s) 81
SsiI CCGC 3 cut(s) 297, 474, 512
SspDI GGCGCC 1 cut(s) 527
SspMI CTAG 2 cut(s) 291, 551
StyD4I CCNGG 2 cut(s) 439, 476
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 3 cut(s) 161, 215, 574
TaqI TCGA 1 cut(s) 305
TasI AATT 1 cut(s) 81
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 89, 492
TscAI CASTG 5 cut(s) 46, 114, 200, 462, 579
TseI GCWGC 3 cut(s) 5, 198, 266
TspRI CASTG 5 cut(s) 46, 114, 200, 462, 579
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XspI CTAG 2 cut(s) 291, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.