FvH4_4g30140

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007655
Physical Location & Seq
Reverse (-)
29923592 .. 29925864
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30140.t1

Sequence Viewer

Length: 582 bp
ATGGCTGCTTTAGTGTATGTAGTGCTTTTCTTGGCCCTCACTGGCCATTCAAGTGCTACTTATTGCTTGTGTAAAGATGGGGTTAGTGATGCTAATCTTCAGAAGGCACTGGACTATGCTTGTGGAGCTGGAGCTGACTGTTCACCAATCCTTTCAAGCGGTACGTGTTACAACCCCAACACAATCAAAGATCACTGCAACTGGGCTGTTAATAGCTACTTCCAGAAAAAGGGTCAAACCCCATTGAGCTGTGACTTTGCTGGAACTGCCACTCAGAGCCAAACTCCTCCAACCACAAGTTCCACTTGTGTTTATCCTTCAAGTGCCAGTAATTCCACCACAAACTCAACCACCACAACTCCAAGCACAACACCAAGCACTACTCCAACCACAGGCACAACCCCAACCGCAACTCCAACCAACGGCACGACCACGGGTACAACAACCCCCAGTACAACTCCATCAGTCTTTGGAATAAGCCCAACGAGTTCTTTCACTGATTCAAGCCCTGGTTTAGCTCCTCACTTCATCGCAGCAGCAGCAACTCTCTGCTTTTCCGCATTTTTGTTGCTATGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

194

Amino Acids

19.67

Weight (kDa)

5.74

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 21 - 91 1.9e-21 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 159, 408, 558
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 3 cut(s) 163, 439, 454
AfiI CCNNNNNNNGG 3 cut(s) 229, 392, 422
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 4 cut(s) 51, 156, 321, 504
AjnI CCWGG 1 cut(s) 508
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 5 cut(s) 128, 134, 216, 249, 518
AluI AGCT 5 cut(s) 128, 134, 216, 249, 518
AoxI GGCC 2 cut(s) 33, 43
ApeKI GCWGC 4 cut(s) 5, 533, 536, 539
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 135
BalI TGGCCA 1 cut(s) 45
BbvI GCAGC 3 cut(s) 545, 548, 551
BccI CCATC 2 cut(s) 71, 469
BceAI ACGGC 1 cut(s) 439
BciT130I CCWGG 1 cut(s) 510
BisI GCNGC 4 cut(s) 6, 534, 537, 540
BlsI GCNGC 4 cut(s) 7, 535, 538, 541
Bme1390I CCNGG 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 510
BmrI ACTGGG 2 cut(s) 211, 444
BmsI GCATC 1 cut(s) 79
BmuI ACTGGG 2 cut(s) 211, 444
BplI GAGNNNNNCTC 2 cut(s) 268, 300
BpmI CTGGAG 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 432, 508
BsaXI ACNNNNNCTCC 4 cut(s) 343, 373, 397, 427
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 392, 422
Bse1I ACTGG 5 cut(s) 46, 114, 206, 327, 450
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 432, 508
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 229, 392, 422
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 5 cut(s) 46, 114, 206, 327, 450
BseRI GAGGAG 2 cut(s) 276, 510
BseXI GCAGC 3 cut(s) 545, 548, 551
BshFI GGCC 2 cut(s) 35, 45
BslI CCNNNNNNNGG 3 cut(s) 229, 392, 422
BsnI GGCC 2 cut(s) 35, 45
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 3 cut(s) 159, 408, 558
BspANI GGCC 2 cut(s) 35, 45
BspCNI CTCAG 1 cut(s) 286
BsrI ACTGG 5 cut(s) 46, 114, 206, 327, 450
BssECI CCNNGG 2 cut(s) 432, 508
BssMI GATC 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 1 cut(s) 432
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 125, 266, 539
BstNI CCWGG 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 508
BstV1I GCAGC 3 cut(s) 545, 548, 551
BsuRI GGCC 2 cut(s) 35, 45
BtgI CCRYGG 1 cut(s) 432
BtgZI GCGATG 1 cut(s) 514
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 4 cut(s) 39, 107, 193, 495
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 162, 438, 453
CviQI GTAC 3 cut(s) 162, 438, 453
DdeI CTNAG 1 cut(s) 273
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 508
FaiI YATR 3 cut(s) 18, 117, 574
FalI AAGNNNNNCTT 4 cut(s) 43, 75, 289, 321
Fnu4HI GCNGC 4 cut(s) 6, 534, 537, 540
Fsp4HI GCNGC 4 cut(s) 6, 534, 537, 540
GluI GCNGC 4 cut(s) 6, 534, 537, 540
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 2 cut(s) 35, 45
HinfI GANTC 1 cut(s) 500
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 2 cut(s) 102, 276
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 97, 327
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 164
HpyCH4V TGCA 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 266, 539
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 164
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 3 cut(s) 125, 131, 523
Lsp1109I GCAGC 3 cut(s) 545, 548, 551
LweI GCATC 1 cut(s) 79
MaeII ACGT 1 cut(s) 164
MaeIII GTNAC 2 cut(s) 167, 251
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 1 cut(s) 89
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 1 cut(s) 331
MluNI TGGCCA 1 cut(s) 45
MmeI TCCRAC 3 cut(s) 314, 410, 440
MnlI CCTC 3 cut(s) 47, 297, 531
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 510
MvaI CCWGG 1 cut(s) 510
MwoI GCNNNNNNNGC 3 cut(s) 125, 266, 539
NdeII GATC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 4 cut(s) 7, 535, 538, 541
Ppu21I YACGTR 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 508
PspGI CCWGG 1 cut(s) 508
PspPI GGNCC 1 cut(s) 34
RsaI GTAC 3 cut(s) 163, 439, 454
RsaNI GTAC 3 cut(s) 162, 438, 453
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 4 cut(s) 6, 534, 537, 540
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 510
SetI ASST 6 cut(s) 130, 136, 167, 218, 251, 520
SfaNI GCATC 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 1 cut(s) 331
SsiI CCGC 3 cut(s) 159, 408, 558
StyD4I CCNGG 1 cut(s) 508
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 1 cut(s) 167
TasI AATT 1 cut(s) 331
TatI WGTACW 1 cut(s) 452
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 4 cut(s) 46, 114, 200, 502
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 4 cut(s) 5, 533, 536, 539
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 517
TspRI CASTG 4 cut(s) 46, 114, 200, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.