pycom13g05560

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007655
Physical Location & Seq
Reverse (-)
3678749 .. 3680416
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05560.1

Sequence Viewer

Length: 636 bp
ATGGCTGCTTTAGTGTATATTGTGATCTTATTGGTCCTCACTGGCCACTCAAGTGCTACTTATTGCGTATGCAAAGATGGAGTCGGTGATGCAACACTTCAGAAGGCACTGGACTATGCCTGTGGAGCTGGAGCTGACTGCAATCCAATTCTCCAAAACGGTGCGTGTTACCAGCCCAACACCGTCAAAGATCACTGCAGCTATGCTGTCAACAGTTATTTCCAGAGGAAGGGTCAAACTGCGCAGAGCTGTGATTTTGCAGGTGCTGCTACTCAGAGCCCAACTGCTCCCAGTACCGCAAGTTCGACATGTGTTTATCCTGCAAGTTCCAGTCAAGCAGGAACTGGCACGCCAACCACAGGCACGCCAACCGGTACACCCACAAACGGCACACCCACCACCCCAACCACAGGCACACCAACCGGCACACCCACCACCCCAACCACGGGCACAACTCCAACCACCGGCACCGGCATCACAACTCCAGGCTCTCCGGCATCAGTGTTCGGAATAAGCCCCACGGGGAGTGGCACTGGAATTGACAACAGTGGCGGCACGGGTAACTTGGAAGCCGCAAACCGCCTCTTCTTCTCTCTAGCTTCGGCTCTGTGCTTCACAGCGGTTTTACTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

212

Amino Acids

20.89

Weight (kDa)

5.38

Isoelectric Point (pI)

25.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 21 - 91 2.2e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 251
Acc16I TGCGCA 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 467
AciI CCGC 5 cut(s) 297, 552, 573, 580, 620
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 2 cut(s) 295, 376
AfiI CCNNNNNNNGG 7 cut(s) 229, 359, 386, 410, 445, 446, 464
AflIII ACRYGT 1 cut(s) 308
AgeI ACCGGT 1 cut(s) 371
AjnI CCWGG 1 cut(s) 484
AleI CACNNNNGTG 1 cut(s) 51
AluBI AGCT 5 cut(s) 128, 134, 201, 249, 599
AluI AGCT 5 cut(s) 128, 134, 201, 249, 599
AlwNI CAGNNNCTG 2 cut(s) 266, 344
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 3 cut(s) 5, 198, 266
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 98
AvaII GGWCC 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 452
BalI TGGCCA 1 cut(s) 45
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 210, 253
BccI CCATC 1 cut(s) 71
BceAI ACGGC 1 cut(s) 403
BciT130I CCWGG 1 cut(s) 486
BfaI CTAG 1 cut(s) 596
BfmI CTRYAG 1 cut(s) 196
BfuAI ACCTGC 1 cut(s) 251
BisI GCNGC 5 cut(s) 6, 199, 267, 553, 573
BlsI GCNGC 5 cut(s) 7, 200, 268, 554, 574
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 1 cut(s) 486
BmrI ACTGGG 1 cut(s) 285
BmsI GCATC 3 cut(s) 79, 483, 506
BmuI ACTGGG 1 cut(s) 285
BpmI CTGGAG 2 cut(s) 150, 468
BpuEI CTTGAG 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 444, 519
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 7 cut(s) 229, 359, 386, 410, 445, 446, 464
Bse118I RCCGGY 4 cut(s) 371, 422, 464, 470
Bse1I ACTGG 6 cut(s) 46, 114, 291, 330, 349, 538
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 444, 519
BseLI CCNNNNNNNGG 7 cut(s) 229, 359, 386, 410, 445, 446, 464
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 6 cut(s) 46, 114, 291, 330, 349, 538
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 2 cut(s) 210, 253
BshFI GGCC 1 cut(s) 45
BshNI GGYRCC 1 cut(s) 467
BshTI ACCGGT 1 cut(s) 371
BsiSI CCGG 5 cut(s) 372, 423, 465, 471, 494
BslI CCNNNNNNNGG 7 cut(s) 229, 359, 386, 410, 445, 446, 464
BsnI GGCC 1 cut(s) 45
Bsp1286I GDGCHC 2 cut(s) 281, 452
Bsp143I GATC 2 cut(s) 24, 190
BspACI CCGC 5 cut(s) 297, 552, 573, 580, 620
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 469
BspMAI CTGCAG 1 cut(s) 200
BspMI ACCTGC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 467
BsrFI RCCGGY 4 cut(s) 371, 422, 464, 470
BsrI ACTGG 6 cut(s) 46, 114, 291, 330, 349, 538
BssAI RCCGGY 4 cut(s) 371, 422, 464, 470
BssECI CCNNGG 2 cut(s) 444, 519
BssMI GATC 2 cut(s) 24, 190
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 4 cut(s) 161, 184, 215, 548
Bst6I CTCTTC 1 cut(s) 590
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 350, 365
BstDEI CTNAG 1 cut(s) 273
BstDSI CCRYGG 2 cut(s) 444, 519
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 2 cut(s) 27, 193
BstMBI GATC 2 cut(s) 24, 190
BstMWI GCNNNNNNNGC 2 cut(s) 125, 266
BstNI CCWGG 1 cut(s) 486
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 210, 253
BsuRI GGCC 1 cut(s) 45
BtgI CCRYGG 2 cut(s) 444, 519
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 6 cut(s) 39, 107, 193, 507, 531, 553
BveI ACCTGC 1 cut(s) 251
Cac8I GCNNGC 2 cut(s) 350, 365
CaiI CAGNNNCTG 2 cut(s) 266, 344
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 4 cut(s) 371, 422, 464, 470
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 294, 375
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 1 cut(s) 309
CviQI GTAC 2 cut(s) 294, 375
DdeI CTNAG 1 cut(s) 273
DpnI GATC 2 cut(s) 26, 192
DpnII GATC 2 cut(s) 24, 190
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 484
EcoT38I GRGCYC 1 cut(s) 281
FaeI CATG 1 cut(s) 312
FaiI YATR 5 cut(s) 18, 70, 117, 204, 310
FalI AAGNNNNNCTT 2 cut(s) 43, 75
FatI CATG 1 cut(s) 308
Fnu4HI GCNGC 5 cut(s) 6, 199, 267, 553, 573
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 5 cut(s) 6, 199, 267, 553, 573
FspBI CTAG 1 cut(s) 596
FspI TGCGCA 1 cut(s) 243
GlaI GCGC 1 cut(s) 243
GluI GCNGC 5 cut(s) 6, 199, 267, 553, 573
GsuI CTGGAG 2 cut(s) 150, 468
HaeIII GGCC 1 cut(s) 45
HapII CCGG 5 cut(s) 372, 423, 465, 471, 494
HhaI GCGC 1 cut(s) 244
Hin1II CATG 1 cut(s) 312
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 1 cut(s) 81
HpaII CCGG 5 cut(s) 372, 423, 465, 471, 494
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 2 cut(s) 211, 377
Hpy188I TCNGA 3 cut(s) 102, 276, 509
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 2 cut(s) 211, 377
HpyAV CCTTC 2 cut(s) 97, 223
HpyCH4III ACNGT 4 cut(s) 161, 184, 215, 548
HpyCH4V TGCA 6 cut(s) 72, 92, 141, 198, 260, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 266
HpyF3I CTNAG 1 cut(s) 273
Hsp92II CATG 1 cut(s) 312
HspAI GCGC 1 cut(s) 242
Kzo9I GATC 2 cut(s) 24, 190
LmnI GCTCC 3 cut(s) 125, 131, 292
Lsp1109I GCAGC 2 cut(s) 210, 253
LweI GCATC 3 cut(s) 79, 483, 506
MaeI CTAG 1 cut(s) 596
MaeIII GTNAC 2 cut(s) 167, 560
MalI GATC 2 cut(s) 26, 192
MboI GATC 2 cut(s) 24, 190
MboII GAAGA 2 cut(s) 577, 580
MhlI GDGCHC 2 cut(s) 281, 452
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 2 cut(s) 147, 537
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 3 cut(s) 47, 219, 593
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 5 cut(s) 372, 423, 465, 471, 494
MspR9I CCNGG 1 cut(s) 486
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 125, 266
NdeII GATC 2 cut(s) 24, 190
NlaIII CATG 1 cut(s) 312
NlaIV GGNNCC 1 cut(s) 469
NsbI TGCGCA 1 cut(s) 243
NspI RCATGY 1 cut(s) 312
OliI CACNNNNGTG 1 cut(s) 51
PaqCI CACCTGC 1 cut(s) 251
PciI ACATGT 1 cut(s) 308
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 5 cut(s) 7, 200, 268, 554, 574
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PscI ACATGT 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 1 cut(s) 34
PsrI GAACNNNNNNTAC 2 cut(s) 286, 318
PstI CTGCAG 1 cut(s) 200
PstNI CAGNNNCTG 2 cut(s) 266, 344
RsaI GTAC 2 cut(s) 295, 376
RsaNI GTAC 2 cut(s) 294, 375
RseI CAYNNNNRTG 1 cut(s) 51
SatI GCNGC 5 cut(s) 6, 199, 267, 553, 573
Sau3AI GATC 2 cut(s) 24, 190
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 2 cut(s) 281, 452
SetI ASST 6 cut(s) 130, 136, 203, 251, 265, 601
SfaNI GCATC 3 cut(s) 79, 483, 506
SfcI CTRYAG 1 cut(s) 196
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 2 cut(s) 147, 537
SsiI CCGC 5 cut(s) 297, 552, 573, 580, 620
SspMI CTAG 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 484
TaaI ACNGT 4 cut(s) 161, 184, 215, 548
TaqI TCGA 1 cut(s) 305
TasI AATT 2 cut(s) 147, 537
TauI GCSGC 2 cut(s) 555, 575
TscAI CASTG 6 cut(s) 46, 114, 200, 507, 538, 553
TseI GCWGC 3 cut(s) 5, 198, 266
TspRI CASTG 6 cut(s) 46, 114, 200, 507, 538, 553
VpaK11BI GGWCC 1 cut(s) 34
XceI RCATGY 1 cut(s) 312
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.