Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Forward (+)

1114127 .. 1117477

Gene Structure 3351 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G03667.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGTGCGGAATTGCAATTGTAGTGTGCGGCGTTCGTATCGAACTCTCGACACTTTCGTCGTCTCTCTCTGAAACTGAGACTCCTTTCGAACGATTACAAGTCTCTGTTGAAGATGTTAAAGCTGTCTTAAGCCAAAGAGGTCCTGATAGTGTAGGAGAAAAGACTATTCATCTTCGACCAAATTGTGGTCAAGAATCTGCTACTCTCTCTGTTGTTGAGACTAGTGAAGGAACTTATAAGCTTGAGGAGACTACTTCTTTGGGTGAATTGCATTTTATTGGTTCTACATTACAGCTTAGAGGAACTAGTCCTATAAGGCAACCATTGGTGGATTCTTCTGGGAACATTCTAGCTTACAATGGCGAAGTATTTGGTGGAATTGAACTCAACAGCTATGATAATGATACTGAAGTGCTTTTGAAGTCTTTAGAGAAAGCAAAATCTTTAGTTCCAGATGTTCTTTCGATGATTAAAGGTCCCTGGGCCATTATATATTGGCAGGAAAGCTCGAGAACGTTATGGTTTGGTAAAGATCCGTTTGGTCGGAGGAGCCTTCTTGTTCACTCGCCAACTGTGGAAGACCCTCGTTTTCTTCTATCGTCTGTTTCACCAGCTTCTTCAGTTTCTCAAGGATTGGATACTGAAAATGGTAATAGCCTTCATAGATATTGGGAAGAGCTTCCATGTGGCGTGTACAGTATTTCTTTTGGGGTTTCAGAATTATGTATTCATGGTGAAGTTACAAAACACGAATGGAGAAATACCATCTGGAAAGAATTGATTGAATGGGAAAGGAAATTAGTTGTACCTAGACCTGAAGATCTATCAACGTCATCGCTTAGTGGAGTTCAGGAAGACAAATCTGTGTCAACTTCTCTAGGATTTGCGCAGACAGTTTTGGTCGTGTTAAAGGAATCAGTGAGGCGGCGAACTTCACTTCATAGCATTTATCAGGGAGATAAAGAAGCTGTCCCAGTAGCTGTTCTTTTCTCTGGTGGATTGGATTCGATGATTCTTGCTGCGCTGTTGCATCAGTGCCTTGATCCAAAATATGAAGTTGATCTCCTTAATGTCAGTTTTGATGGTCCTAATGCTCCTGATAGGATCTCCGCCAAGGCTGGAATAAAGGAACTCAAAAAGATTGCACCTTTTAGAAGGTGGAAACTTGTTGAGATTGATGCTGATCTGTCAAAACTAGGCTTTGAGACAAAACGTGTTATGTCACTCATAAATCCTGCAGACACTTACATGGACCTTAACATAGGAACAGCATTATGGTTAGCTGCTCGTGGTGATGGTTGGATTCACGAAGATAATGGAAATCCAAGTGTAGAGGAAAATAACCAGCGGGTCAAGTATAAGTCCGATGCTAGAATCCTGCTTGTTGGCGCTGGTGCTGATGAGCAATGTGCCGGTTACGGTAGGCACAGAACTAAATATCGAAATGGAAGTTGGGTTGCGTTGGATCAAGAAATGAAATTAGATATGCAGAGAATTTGGAAAAGAAATTTGGGTCGAGATGATCGATGCATTGCAGATAACGGTAAAGAGGGGCGATTCCCGTTCTTAGATGAAGATGTGATAAAGACTCTCCTTGACATTCCCTTGTGGGAAATCGCTGATCTTGAGCAACCAAGTGGAACGGGAGACAAGAAGATTCTTAGACAGGTTGCAAGCCTGCTTGGTGTACATGAAGTTGCAAAGATGCCTAAGAGAGCAATTCAGTTTGGATCAAGAATCGCTCGTGAATCAAACCGGAAGAATTTTGGAAGTAACCGTGCCGCAAACCAGGCTTCTGCCGGGAGCGTGAGATTCCTAGCACCGTCACACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

610

Amino Acids

67.65

Weight (kDa)

5.95

Isoelectric Point (pI)

40.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	106 - 201	3.2e-08	Glutamine amidotransferase domain
Asn_synthase	PF00733	483 - 576	3.2e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	237
AasI	GACNNNNNNGTC	1 cut(s)	55
Acc16I	TGCGCA	1 cut(s)	888
AccB7I	CCANNNNNTGG	1 cut(s)	185
AciI	CCGC	6 cut(s)	6, 27, 925, 1110, 1348, 1782
AclI	AACGTT	1 cut(s)	515
AclWI	GGATC	5 cut(s)	527, 1037, 1112, 1473, 1738
AcsI	RAATTY	3 cut(s)	1494, 1507, 1762
AcuI	CTGAAG	3 cut(s)	429, 603, 837
AfaI	GTAC	3 cut(s)	695, 807, 1689
AfiI	CCNNNNNNNGG	1 cut(s)	185
AflII	CTTAAG	1 cut(s)	127
AflIII	ACRYGT	1 cut(s)	1213
AgsI	TTSAA	4 cut(s)	110, 383, 421, 785
AhlI	ACTAGT	2 cut(s)	221, 305
AjnI	CCWGG	2 cut(s)	479, 1788
AjuI	GAANNNNNNNTTGG	4 cut(s)	1435, 1467, 1493, 1525
Alw26I	GTCTC	7 cut(s)	66, 71, 106, 212, 242, 1199, 1641
AlwI	GGATC	5 cut(s)	527, 1037, 1112, 1473, 1738
AlwNI	CAGNNNCTG	1 cut(s)	980
Ama87I	CYCGRG	1 cut(s)	508
AoxI	GGCC	1 cut(s)	483
ApeKI	GCWGC	2 cut(s)	1019, 1283
ApoI	RAATTY	3 cut(s)	1494, 1507, 1762
ArsI	GACNNNNNNTTYG	2 cut(s)	241, 273
Asp700I	GAANNNNTTC	1 cut(s)	678
AspLEI	GCGC	3 cut(s)	889, 1024, 1391
AspS9I	GGNCC	5 cut(s)	140, 476, 483, 1085, 1252
AsuC2I	CCSGG	1 cut(s)	1801
AsuHPI	GGTGA	4 cut(s)	275, 600, 746, 1304
AsuII	TTCGAA	1 cut(s)	87
AvaI	CYCGRG	1 cut(s)	508
AvaII	GGWCC	4 cut(s)	140, 476, 1085, 1252
BaeI	ACNNNNGTAYC	2 cut(s)	396, 429
BauI	CACGAG	2 cut(s)	1287, 1743
BbsI	GAAGAC	2 cut(s)	585, 861
BbvI	GCAGC	2 cut(s)	1006, 1270
BccI	CCATC	3 cut(s)	773, 1076, 1289
BciT130I	CCWGG	2 cut(s)	481, 1790
BciVI	GTATCC	1 cut(s)	631
BcnI	CCSGG	1 cut(s)	1801
BcoDI	GTCTC	7 cut(s)	66, 71, 106, 212, 242, 1199, 1641
BcuI	ACTAGT	2 cut(s)	221, 305
BfaI	CTAG	8 cut(s)	222, 306, 350, 810, 878, 1196, 1371, 1817
BfmI	CTRYAG	1 cut(s)	1236
BfoI	RGCGCY	1 cut(s)	1392
BfrI	CTTAAG	1 cut(s)	127
BfuI	GTATCC	1 cut(s)	631
BglII	AGATCT	1 cut(s)	820
BisI	GCNGC	5 cut(s)	28, 926, 1020, 1284, 1782
BlsI	GCNGC	5 cut(s)	29, 927, 1021, 1285, 1783
Bme1390I	CCNGG	3 cut(s)	481, 1790, 1801
Bme18I	GGWCC	4 cut(s)	140, 476, 1085, 1252
BmeT110I	CYCGRG	1 cut(s)	508
BmgT120I	GGNCC	5 cut(s)	140, 476, 483, 1085, 1252
BmiI	GGNNCC	2 cut(s)	478, 551
BmrFI	CCNGG	3 cut(s)	481, 1790, 1801
BmrI	ACTGGG	1 cut(s)	968
BmsI	GCATC	5 cut(s)	1039, 1168, 1357, 1517, 1695
BmuI	ACTGGG	1 cut(s)	968
BpiI	GAAGAC	2 cut(s)	585, 861
Bpu14I	TTCGAA	1 cut(s)	87
BpuEI	CTTGAG	3 cut(s)	263, 612, 1646
BpuMI	CCSGG	1 cut(s)	1801
Bsa29I	ATCGAT	1 cut(s)	1525
BsaBI	GATNNNNATC	1 cut(s)	1182
BsaJI	CCNNGG	3 cut(s)	479, 480, 1113
BsaWI	WCCGGW	1 cut(s)	1755
BsaXI	ACNNNNNCTCC	2 cut(s)	64, 94
Bsc4I	CCNNNNNNNGG	1 cut(s)	185
Bse118I	RCCGGY	1 cut(s)	1412
Bse1I	ACTGG	1 cut(s)	974
Bse3DI	GCAATG	2 cut(s)	1412, 1530
Bse8I	GATNNNNATC	1 cut(s)	1182
BseBI	CCWGG	2 cut(s)	481, 1790
BseCI	ATCGAT	1 cut(s)	1525
BseDI	CCNNGG	3 cut(s)	479, 480, 1113
BseJI	GATNNNNATC	1 cut(s)	1182
BseLI	CCNNNNNNNGG	1 cut(s)	185
BseMI	GCAATG	2 cut(s)	1412, 1530
BseMII	CTCAG	1 cut(s)	66
BseNI	ACTGG	1 cut(s)	974
BseRI	GAGGAG	2 cut(s)	260, 562
BseXI	GCAGC	2 cut(s)	1006, 1270
BshFI	GGCC	1 cut(s)	485
BshVI	ATCGAT	1 cut(s)	1525
BsiHKCI	CYCGRG	1 cut(s)	508
BsiSI	CCGG	3 cut(s)	1413, 1756, 1800
BslFI	GGGAC	2 cut(s)	462, 956
BslI	CCNNNNNNNGG	1 cut(s)	185
BsmAI	GTCTC	7 cut(s)	66, 71, 106, 212, 242, 1199, 1641
BsmBI	CGTCTC	1 cut(s)	66
BsmFI	GGGAC	2 cut(s)	462, 956
BsnI	GGCC	1 cut(s)	485
BsoBI	CYCGRG	1 cut(s)	508
Bsp119I	TTCGAA	1 cut(s)	87
Bsp1407I	TGTACA	2 cut(s)	693, 1687
BspACI	CCGC	6 cut(s)	6, 27, 925, 1110, 1348, 1782
BspANI	GGCC	1 cut(s)	485
BspCNI	CTCAG	1 cut(s)	67
BspDI	ATCGAT	1 cut(s)	1525
BspLI	GGNNCC	2 cut(s)	478, 551
BspMAI	CTGCAG	1 cut(s)	1240
BspPI	GGATC	5 cut(s)	527, 1037, 1112, 1473, 1738
BspQI	GCTCTTC	1 cut(s)	669
BspT104I	TTCGAA	1 cut(s)	87
BspTI	CTTAAG	1 cut(s)	127
BsrDI	GCAATG	2 cut(s)	1412, 1530
BsrFI	RCCGGY	1 cut(s)	1412
BsrGI	TGTACA	2 cut(s)	693, 1687
BsrI	ACTGG	1 cut(s)	974
BssAI	RCCGGY	1 cut(s)	1412
BssECI	CCNNGG	3 cut(s)	479, 480, 1113
BssSI	CACGAG	2 cut(s)	1287, 1743
BssT1I	CCWWGG	1 cut(s)	1113
Bst2BI	CACGAG	2 cut(s)	1287, 1743
Bst2UI	CCWGG	2 cut(s)	481, 1790
Bst4CI	ACNGT	7 cut(s)	574, 698, 895, 1421, 1544, 1778, 1824
Bst6I	CTCTTC	1 cut(s)	669
BstAFI	CTTAAG	1 cut(s)	127
BstAUI	TGTACA	2 cut(s)	693, 1687
BstBI	TTCGAA	1 cut(s)	87
BstC8I	GCNNGC	2 cut(s)	1675, 1679
BstDEI	CTNAG	6 cut(s)	75, 296, 839, 1567, 1661, 1710
BstH2I	RGCGCY	1 cut(s)	1392
BstHHI	GCGC	3 cut(s)	889, 1024, 1391
BstMAI	GTCTC	7 cut(s)	66, 71, 106, 212, 242, 1199, 1641
BstMWI	GCNNNNNNNGC	1 cut(s)	1790
BstNI	CCWGG	2 cut(s)	481, 1790
BstSCI	CCNGG	3 cut(s)	479, 1788, 1799
BstSFI	CTRYAG	1 cut(s)	1236
BstV1I	GCAGC	2 cut(s)	1006, 1270
BstV2I	GAAGAC	2 cut(s)	585, 861
BstX2I	RGATCY	3 cut(s)	532, 820, 1104
BstYI	RGATCY	3 cut(s)	532, 820, 1104
Bsu15I	ATCGAT	1 cut(s)	1525
BsuI	GTATCC	1 cut(s)	631
BsuRI	GGCC	1 cut(s)	485
BsuTUI	ATCGAT	1 cut(s)	1525
BtgZI	GCGATG	1 cut(s)	819
BtsIMutI	CAGTG	2 cut(s)	924, 1040
Cac8I	GCNNGC	2 cut(s)	1675, 1679
CaiI	CAGNNNCTG	1 cut(s)	980
CfoI	GCGC	3 cut(s)	889, 1024, 1391
Cfr10I	RCCGGY	1 cut(s)	1412
Cfr13I	GGNCC	5 cut(s)	140, 476, 483, 1085, 1252
ClaI	ATCGAT	1 cut(s)	1525
Csp6I	GTAC	3 cut(s)	694, 806, 1688
CviAII	CATG	4 cut(s)	684, 731, 1249, 1691
CviQI	GTAC	3 cut(s)	694, 806, 1688
DdeI	CTNAG	6 cut(s)	75, 296, 839, 1567, 1661, 1710
DrdI	GACNNNNNNGTC	1 cut(s)	55
DseDI	GACNNNNNNGTC	1 cut(s)	55
Eam1104I	CTCTTC	1 cut(s)	669
EarI	CTCTTC	1 cut(s)	669
EciI	GGCGGA	1 cut(s)	1099
Eco130I	CCWWGG	1 cut(s)	1113
Eco47I	GGWCC	4 cut(s)	140, 476, 1085, 1252
Eco57I	CTGAAG	3 cut(s)	429, 603, 837
Eco88I	CYCGRG	1 cut(s)	508
EcoO109I	RGGNCCY	2 cut(s)	140, 476
EcoRII	CCWGG	2 cut(s)	479, 1788
EcoT14I	CCWWGG	1 cut(s)	1113
EcoT22I	ATGCAT	1 cut(s)	1532
ErhI	CCWWGG	1 cut(s)	1113
Esp3I	CGTCTC	1 cut(s)	66
FaeI	CATG	4 cut(s)	687, 734, 1252, 1694
FaqI	GGGAC	2 cut(s)	462, 956
FatI	CATG	4 cut(s)	683, 730, 1248, 1690
FauI	CCCGC	1 cut(s)	1341
Fnu4HI	GCNGC	5 cut(s)	28, 926, 1020, 1284, 1782
Fsp4HI	GCNGC	5 cut(s)	28, 926, 1020, 1284, 1782
FspBI	CTAG	8 cut(s)	222, 306, 350, 810, 878, 1196, 1371, 1817
FspI	TGCGCA	1 cut(s)	888
GlaI	GCGC	3 cut(s)	888, 1023, 1390
GluI	GCNGC	5 cut(s)	28, 926, 1020, 1284, 1782
HaeII	RGCGCY	1 cut(s)	1392
HaeIII	GGCC	1 cut(s)	485
HapII	CCGG	3 cut(s)	1413, 1756, 1800
HhaI	GCGC	3 cut(s)	889, 1024, 1391
Hin1II	CATG	4 cut(s)	687, 734, 1252, 1694
Hin6I	GCGC	3 cut(s)	887, 1022, 1389
HinP1I	GCGC	3 cut(s)	887, 1022, 1389
HincII	GTYRAC	1 cut(s)	870
HindII	GTYRAC	1 cut(s)	870
HindIII	AAGCTT	1 cut(s)	239
HpaII	CCGG	3 cut(s)	1413, 1756, 1800
HphI	GGTGA	4 cut(s)	275, 600, 746, 1304
Hpy166II	GTNNAC	4 cut(s)	562, 694, 870, 1688
Hpy188I	TCNGA	4 cut(s)	70, 546, 718, 1366
Hpy8I	GTNNAC	4 cut(s)	562, 694, 870, 1688
Hpy99I	CGWCG	1 cut(s)	61
HpyAV	CCTTC	4 cut(s)	221, 563, 668, 1149
HpyCH4III	ACNGT	7 cut(s)	574, 698, 895, 1421, 1544, 1778, 1824
HpyCH4IV	ACGT	3 cut(s)	515, 830, 1213
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1790
HpyF3I	CTNAG	6 cut(s)	75, 296, 839, 1567, 1661, 1710
HpySE526I	ACGT	3 cut(s)	515, 830, 1213
Hsp92II	CATG	4 cut(s)	687, 734, 1252, 1694
HspAI	GCGC	3 cut(s)	887, 1022, 1389
LguI	GCTCTTC	1 cut(s)	669
LmnI	GCTCC	3 cut(s)	549, 1099, 1803
Lsp1109I	GCAGC	2 cut(s)	1006, 1270
LweI	GCATC	5 cut(s)	1039, 1168, 1357, 1517, 1695
MaeI	CTAG	8 cut(s)	222, 306, 350, 810, 878, 1196, 1371, 1817
MaeII	ACGT	3 cut(s)	515, 830, 1213
MaeIII	GTNAC	5 cut(s)	739, 1221, 1415, 1772, 1824
MfeI	CAATTG	1 cut(s)	15
MflI	RGATCY	3 cut(s)	532, 820, 1104
MlyI	GAGTC	2 cut(s)	73, 1582
MmeI	TCCRAC	3 cut(s)	524, 1280, 1443
MnlI	CCTC	8 cut(s)	131, 238, 293, 540, 594, 915, 1327, 1543
Mph1103I	ATGCAT	1 cut(s)	1532
MroXI	GAANNNNTTC	1 cut(s)	678
MseI	TTAA	6 cut(s)	117, 128, 471, 908, 1068, 1257
MslI	CAYNNNNRTG	1 cut(s)	1247
MspA1I	CMGCKG	1 cut(s)	1348
MspCI	CTTAAG	1 cut(s)	127
MspI	CCGG	3 cut(s)	1413, 1756, 1800
MspR9I	CCNGG	3 cut(s)	481, 1790, 1801
MunI	CAATTG	1 cut(s)	15
MvaI	CCWGG	2 cut(s)	481, 1790
MwoI	GCNNNNNNNGC	1 cut(s)	1790
NciI	CCSGG	1 cut(s)	1801
NlaIII	CATG	4 cut(s)	687, 734, 1252, 1694
NlaIV	GGNNCC	2 cut(s)	478, 551
NmuCI	GTSAC	2 cut(s)	1221, 1824
NsbI	TGCGCA	1 cut(s)	888
NsiI	ATGCAT	1 cut(s)	1532
NspV	TTCGAA	1 cut(s)	87
PaeR7I	CTCGAG	1 cut(s)	508
PasI	CCCWGGG	1 cut(s)	480
PciSI	GCTCTTC	1 cut(s)	669
PcsI	WCGNNNNNNNCGW	2 cut(s)	53, 1522
PdmI	GAANNNNTTC	1 cut(s)	678
PflMI	CCANNNNNTGG	1 cut(s)	185
PkrI	GCNGC	5 cut(s)	29, 927, 1021, 1285, 1783
PleI	GAGTC	2 cut(s)	73, 1582
PpsI	GAGTC	2 cut(s)	73, 1582
PpuMI	RGGWCCY	2 cut(s)	140, 476
PsiI	TTATAA	1 cut(s)	237
Psp1406I	AACGTT	1 cut(s)	515
Psp5II	RGGWCCY	2 cut(s)	140, 476
Psp6I	CCWGG	2 cut(s)	479, 1788
PspGI	CCWGG	2 cut(s)	479, 1788
PspN4I	GGNNCC	2 cut(s)	478, 551
PspPI	GGNCC	5 cut(s)	140, 476, 483, 1085, 1252
PspPPI	RGGWCCY	2 cut(s)	140, 476
PstI	CTGCAG	1 cut(s)	1240
PstNI	CAGNNNCTG	1 cut(s)	980
PsuI	RGATCY	3 cut(s)	532, 820, 1104
RsaI	GTAC	3 cut(s)	695, 807, 1689
RsaNI	GTAC	3 cut(s)	694, 806, 1688
RseI	CAYNNNNRTG	1 cut(s)	1247
SapI	GCTCTTC	1 cut(s)	669
SaqAI	TTAA	6 cut(s)	117, 128, 471, 908, 1068, 1257
SatI	GCNGC	5 cut(s)	28, 926, 1020, 1284, 1782
Sau96I	GGNCC	5 cut(s)	140, 476, 483, 1085, 1252
SchI	GAGTC	2 cut(s)	73, 1582
ScrFI	CCNGG	3 cut(s)	481, 1790, 1801
SfaNI	GCATC	5 cut(s)	1039, 1168, 1357, 1517, 1695
SfcI	CTRYAG	1 cut(s)	1236
Sfr274I	CTCGAG	1 cut(s)	508
SfuI	TTCGAA	1 cut(s)	87
SinI	GGWCC	4 cut(s)	140, 476, 1085, 1252
SlaI	CTCGAG	1 cut(s)	508
SmiMI	CAYNNNNRTG	1 cut(s)	1247
SmlI	CTYRAG	5 cut(s)	127, 242, 508, 627, 1625
SmoI	CTYRAG	5 cut(s)	127, 242, 508, 627, 1625
SpeI	ACTAGT	2 cut(s)	221, 305
SsiI	CCGC	6 cut(s)	6, 27, 925, 1110, 1348, 1782
SspMI	CTAG	8 cut(s)	222, 306, 350, 810, 878, 1196, 1371, 1817
StyD4I	CCNGG	3 cut(s)	479, 1788, 1799
StyI	CCWWGG	1 cut(s)	1113
TaaI	ACNGT	7 cut(s)	574, 698, 895, 1421, 1544, 1778, 1824
TaiI	ACGT	3 cut(s)	518, 833, 1216
TatI	WGTACW	2 cut(s)	693, 1687
TauI	GCSGC	3 cut(s)	30, 928, 1784
Tru1I	TTAA	6 cut(s)	117, 128, 471, 908, 1068, 1257
Tru9I	TTAA	6 cut(s)	117, 128, 471, 908, 1068, 1257
TscAI	CASTG	2 cut(s)	924, 1040
TseFI	GTSAC	2 cut(s)	1221, 1824
TseI	GCWGC	2 cut(s)	1019, 1283
Tsp45I	GTSAC	2 cut(s)	1221, 1824
TspDTI	ATGAA	8 cut(s)	158, 650, 719, 929, 1068, 1490, 1587, 1707
TspGWI	ACGGA	1 cut(s)	525
TspRI	CASTG	2 cut(s)	924, 1040
Van91I	CCANNNNNTGG	1 cut(s)	185
Vha464I	CTTAAG	1 cut(s)	127
VpaK11BI	GGWCC	4 cut(s)	140, 476, 1085, 1252
XapI	RAATTY	3 cut(s)	1494, 1507, 1762
XhoI	CTCGAG	1 cut(s)	508
XmnI	GAANNNNTTC	1 cut(s)	678
XspI	CTAG	8 cut(s)	222, 306, 350, 810, 878, 1196, 1371, 1817
Zsp2I	ATGCAT	1 cut(s)	1532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.