Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

60996357 .. 61006508

Gene Structure 10152 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ40630

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

ATGGCTTCCGCGTTTGCCTTTGAGTTAGGCGCGCATCGCATTCTAACAAAGATGTGCGGAATCGCTCTGATCATCTCCGGCATTCGCTTCGACTTATCGTCTCTGCTTCTGGACACCACACCTCCAGTCACCAACTCCGACCAACTAGAGTTCACTATAGCCGACCTCGCAGCCGCTCTGCGACGGAGGGGCCCAGATAGCTTAGGCACCAAGAAGCTTCTTCTTCACTCATCAGGCTCGGAAATCATGTCTGGAGTAGAGGAGGAGTTCAACAACTGCTTCTCTGCGGCAAATGGTCGAACACCCTGTATGCATTTTTTTGGTGCTACTTTGCAGCTCAGGGGAGTCAACCCAGTAGTTCACCCATTGATAGATTCGTCTAAGAACGTTCTTGTCTATAATGGTGAGATCTTTGGGGGGATTGAGATTGCTAGTAAGGAGAATGACGGTGAAGTTCTAATGGGGTTGCTGGGAGGGTGTGAAGGTTGTGTGCCGAATGTTGTTTCTAGGATCAAGGGCCCTTGGGCTATCATCTATTGGCAGGAAAGTTCAAGAACATTATGGTTTGGGAGAGATGCATTTGGTCGCCGGAGTCTTCTTGTTCACTGGCCAACAGTGGAGGACTCTCGGTTTCTGTTATCATCAGTATCACCCGTTGCTTCAAATGAGCATTCTTCTGCTGAAAATGGAACAACCAAGCTTGGTTTTTGGGAGGAGCTGCCATGTGGAATATACAGTGTATCCATAGATGCTCCCAATGGTGACGGGAGTTTGGTTGGTGAAGTGAAAAAGCATGAATGGACTAATGCCATGCTAGAAGAACTGATAAAATGGGAGAGAACATCTGTCGAACCCAAACCCAAAGAGTTGCATATTTCACGTTGTGAGACTGTAAGAGGGCAACACGATATGAACTCACTTACTTCAGATATAGTGCCAATGCCATCTAAGTCAGGCAATATACAAGCTTCAATTTCAGTGCCAGCAGAGGCTGTGCTGATTGCTTTAAGGGAATCAGTTTTGCGGCGATCTTCACTGCACAGAATTTTTCAGGCAAGTATGCATGTGGAAAAGAATCTTGTACCGGTGGCAGTTCTTTTTTCTGGTGGATTGGATTCTATGATAATTGCAGCATTGTTGCATGACTGCCTAGATCCAAGCTATGAGATTGATCTACTTAATGTGAGCTTTGATGGTCAGTCTGCTCCAGATAGAATCTCTGCCAAGGCAGGAGTGAGCGAACTGAGAAGAATTGCACCTTTAAGAAAGTGGAAACTTGTGGAGATCAATGCTGATTCATCAAATTTGACCTTTGAAACAAAGCGTGTATTGTCTCTCATAAACCCTGCCAACACCTACATGGACCTAAATATAGGAATCGCGTTATGGCTGGCAGCTGGTGGGGATGGATGGGTTTATGAGGAAAATAGCAAATATAATGATGAGAATCGTCAAGGTGTTAGATACAAGTCAAAGGCTAGGATTGTCCTTGTTGGTTCAGGTGCAGATGAGCAATGTGCTGGCTATGGTAGGCACAGAACAAAATATAGAAGTGGAAGTTGGTTGCGACTGCATGAGGAAATGAAACTGGATATGCAAAGAATATGGAAAAGAAACCTTGGTAGAGATGATAGATGCATTGCTGACACTGGCAAGGAGGCAAGGTTTCCTTTTTTGGATGAAGATGTTATACGAACTTTATTGGGGATTCCTCTCTGGGAGGTTACCAACCTTGATCAACCAAGTGGAATAGGAGATAAGAAGATTTTGAGAGAGGTTGCGGAATTGCTTGGTTTACATGAAGCTGCTACTCTGCCTAAACGAGCTATACAGTTTGGTACAAGAATTGCAAGGGAGTCCAATAGAAAGAATTTCGGAAGTAACCGTGCTGCAAATCAGGCATCTGCGGGAAGTGTGGTGATTCACAAGCAGTTGGACACGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

648

Amino Acids

71.46

Weight (kDa)

6.49

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	122 - 215	1.7e-07	Glutamine amidotransferase domain
Asn_synthase	PF00733	518 - 608	1.7e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	206
AccBSI	CCGCTC	1 cut(s)	176
AccII	CGCG	3 cut(s)	11, 32, 1382
AciI	CCGC	7 cut(s)	9, 57, 174, 287, 1024, 1781, 1907
AclI	AACGTT	1 cut(s)	387
AclWI	GGATC	2 cut(s)	518, 1148
AcoI	YGGCCR	1 cut(s)	608
AcsI	RAATTY	3 cut(s)	1044, 1303, 1870
AcuI	CTGAAG	1 cut(s)	909
AdeI	CACNNNGTG	1 cut(s)	884
AfaI	GTAC	2 cut(s)	1083, 1840
AgeI	ACCGGT	1 cut(s)	1084
AgsI	TTSAA	5 cut(s)	271, 552, 663, 972, 1316
AhdI	GACNNNNNGTC	1 cut(s)	97
AjuI	GAANNNNNNNTTGG	2 cut(s)	1543, 1575
Alw26I	GTCTC	3 cut(s)	105, 881, 1338
AlwI	GGATC	2 cut(s)	518, 1148
AlwNI	CAGNNNCTG	1 cut(s)	992
AoxI	GGCC	3 cut(s)	190, 517, 608
ApaI	GGGCCC	2 cut(s)	194, 521
ApeKI	GCWGC	7 cut(s)	170, 334, 718, 1130, 1394, 1805, 1889
ApoI	RAATTY	3 cut(s)	1044, 1303, 1870
AsiGI	ACCGGT	1 cut(s)	1084
AspLEI	GCGC	2 cut(s)	32, 34
AspS9I	GGNCC	5 cut(s)	190, 191, 517, 518, 1363
AsuHPI	GGTGA	8 cut(s)	121, 353, 416, 461, 642, 773, 791, 1930
AvaII	GGWCC	1 cut(s)	1363
BaeGI	GKGCMC	2 cut(s)	194, 521
BalI	TGGCCA	1 cut(s)	610
BanI	GGYRCC	1 cut(s)	206
BanII	GRGCYC	2 cut(s)	194, 521
BarI	GAAGNNNNNNTAC	2 cut(s)	1674, 1706
BbsI	GAAGAC	1 cut(s)	587
BbvI	GCAGC	7 cut(s)	182, 346, 705, 1142, 1406, 1792, 1876
BccI	CCATC	4 cut(s)	952, 1187, 1400, 1404
BcgI	CGANNNNNNTGC	2 cut(s)	70, 104
BciVI	GTATCC	1 cut(s)	751
BclI	TGATCA	2 cut(s)	69, 1735
BcoDI	GTCTC	3 cut(s)	105, 881, 1338
BfaI	CTAG	6 cut(s)	146, 432, 507, 815, 1151, 1479
BfmI	CTRYAG	1 cut(s)	156
BfuI	GTATCC	1 cut(s)	751
BglII	AGATCT	1 cut(s)	408
Bme18I	GGWCC	1 cut(s)	1363
BmeRI	GACNNNNNGTC	1 cut(s)	97
BmgT120I	GGNCC	5 cut(s)	190, 191, 517, 518, 1363
BmiI	GGNNCC	4 cut(s)	191, 192, 208, 519
BmrI	ACTGGG	1 cut(s)	347
BmsI	GCATC	5 cut(s)	43, 565, 739, 1625, 1910
BmuI	ACTGGG	1 cut(s)	347
BpiI	GAAGAC	1 cut(s)	587
BpmI	CTGGAG	3 cut(s)	108, 273, 1191
Bpu10I	CCTNAGC	2 cut(s)	202, 338
BsaBI	GATNNNNATC	1 cut(s)	1446
BsaJI	CCNNGG	3 cut(s)	521, 1224, 1618
BsaWI	WCCGGW	1 cut(s)	1084
BsaXI	ACNNNNNCTCC	4 cut(s)	106, 136, 1649, 1679
Bse118I	RCCGGY	1 cut(s)	1084
Bse1I	ACTGG	5 cut(s)	125, 353, 611, 1593, 1654
Bse3DI	GCAATG	2 cut(s)	1520, 1638
Bse8I	GATNNNNATC	1 cut(s)	1446
BseDI	CCNNGG	3 cut(s)	521, 1224, 1618
BseGI	GGATG	3 cut(s)	1411, 1415, 1684
BseJI	GATNNNNATC	1 cut(s)	1446
BseMI	GCAATG	2 cut(s)	1520, 1638
BseMII	CTCAG	2 cut(s)	352, 1235
BseNI	ACTGG	5 cut(s)	125, 353, 611, 1593, 1654
BsePI	GCGCGC	1 cut(s)	30
BseRI	GAGGAG	3 cut(s)	275, 278, 728
BseSI	GKGCMC	2 cut(s)	194, 521
BseXI	GCAGC	7 cut(s)	182, 346, 705, 1142, 1406, 1792, 1876
BseYI	CCCAGC	1 cut(s)	469
BsgI	GTGCAG	2 cut(s)	1022, 1524
Bsh1236I	CGCG	3 cut(s)	11, 32, 1382
BshFI	GGCC	3 cut(s)	192, 519, 610
BshNI	GGYRCC	1 cut(s)	206
BshTI	ACCGGT	1 cut(s)	1084
BsiSI	CCGG	3 cut(s)	78, 589, 1085
BsmAI	GTCTC	3 cut(s)	105, 881, 1338
BsmBI	CGTCTC	1 cut(s)	105
BsmI	GAATGC	3 cut(s)	39, 81, 670
BsnI	GGCC	3 cut(s)	192, 519, 610
Bsp120I	GGGCCC	2 cut(s)	190, 517
Bsp1286I	GDGCHC	2 cut(s)	194, 521
Bsp143I	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
BspACI	CCGC	7 cut(s)	9, 57, 174, 287, 1024, 1781, 1907
BspANI	GGCC	3 cut(s)	192, 519, 610
BspCNI	CTCAG	2 cut(s)	351, 1236
BspFNI	CGCG	3 cut(s)	11, 32, 1382
BspLI	GGNNCC	4 cut(s)	191, 192, 208, 519
BspPI	GGATC	2 cut(s)	518, 1148
BspT107I	GGYRCC	1 cut(s)	206
BsrBI	CCGCTC	1 cut(s)	176
BsrDI	GCAATG	2 cut(s)	1520, 1638
BsrFI	RCCGGY	1 cut(s)	1084
BsrI	ACTGG	5 cut(s)	125, 353, 611, 1593, 1654
BssAI	RCCGGY	1 cut(s)	1084
BssECI	CCNNGG	3 cut(s)	521, 1224, 1618
BssHII	GCGCGC	1 cut(s)	30
BssMI	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
BssT1I	CCWWGG	3 cut(s)	521, 1224, 1618
Bst4CI	ACNGT	6 cut(s)	449, 616, 737, 892, 1833, 1886
BstC8I	GCNNGC	4 cut(s)	32, 984, 1392, 1522
BstDEI	CTNAG	6 cut(s)	202, 338, 381, 948, 1244, 1944
BstEII	GGTNACC	1 cut(s)	1723
BstF5I	GGATG	3 cut(s)	1411, 1415, 1684
BstFNI	CGCG	3 cut(s)	11, 32, 1382
BstHHI	GCGC	2 cut(s)	32, 34
BstKTI	GATC	8 cut(s)	72, 411, 513, 1031, 1156, 1174, 1287, 1738
BstMAI	GTCTC	3 cut(s)	105, 881, 1338
BstMBI	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
BstMWI	GCNNNNNNNGC	4 cut(s)	36, 167, 198, 1898
BstNSI	RCATGY	1 cut(s)	1067
BstPI	GGTNACC	1 cut(s)	1723
BstSFI	CTRYAG	1 cut(s)	156
BstSLI	GKGCMC	2 cut(s)	194, 521
BstUI	CGCG	3 cut(s)	11, 32, 1382
BstV1I	GCAGC	7 cut(s)	182, 346, 705, 1142, 1406, 1792, 1876
BstV2I	GAAGAC	1 cut(s)	587
BstX2I	RGATCY	2 cut(s)	408, 1153
BstYI	RGATCY	2 cut(s)	408, 1153
BsuI	GTATCC	1 cut(s)	751
BsuRI	GGCC	3 cut(s)	192, 519, 610
BtgZI	GCGATG	1 cut(s)	20
BtsCI	GGATG	3 cut(s)	1411, 1415, 1684
BtsI	GCAGTG	1 cut(s)	1034
BtsIMutI	CAGTG	6 cut(s)	604, 621, 742, 984, 1034, 1647
Cac8I	GCNNGC	4 cut(s)	32, 984, 1392, 1522
CaiI	CAGNNNCTG	1 cut(s)	992
CfoI	GCGC	2 cut(s)	32, 34
Cfr10I	RCCGGY	1 cut(s)	1084
Cfr13I	GGNCC	5 cut(s)	190, 191, 517, 518, 1363
Csp6I	GTAC	2 cut(s)	1082, 1839
CspAI	ACCGGT	1 cut(s)	1084
CviAII	CATG	9 cut(s)	247, 723, 794, 811, 1064, 1142, 1360, 1574, 1799
CviQI	GTAC	2 cut(s)	1082, 1839
DdeI	CTNAG	6 cut(s)	202, 338, 381, 948, 1244, 1944
DpnI	GATC	8 cut(s)	71, 410, 512, 1030, 1155, 1173, 1286, 1737
DpnII	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
DraIII	CACNNNGTG	1 cut(s)	884
DriI	GACNNNNNGTC	1 cut(s)	97
EaeI	YGGCCR	1 cut(s)	608
Eam1105I	GACNNNNNGTC	1 cut(s)	97
Eco130I	CCWWGG	3 cut(s)	521, 1224, 1618
Eco24I	GRGCYC	2 cut(s)	194, 521
Eco47I	GGWCC	1 cut(s)	1363
Eco57I	CTGAAG	1 cut(s)	909
Eco91I	GGTNACC	1 cut(s)	1723
EcoO109I	RGGNCCY	3 cut(s)	190, 517, 518
EcoO65I	GGTNACC	1 cut(s)	1723
EcoT14I	CCWWGG	3 cut(s)	521, 1224, 1618
EcoT22I	ATGCAT	4 cut(s)	315, 580, 1065, 1640
EcoT38I	GRGCYC	2 cut(s)	194, 521
ErhI	CCWWGG	3 cut(s)	521, 1224, 1618
Esp3I	CGTCTC	1 cut(s)	105
FaeI	CATG	9 cut(s)	250, 726, 797, 814, 1067, 1145, 1363, 1577, 1802
FalI	AAGNNNNNCTT	2 cut(s)	1654, 1686
FatI	CATG	9 cut(s)	246, 722, 793, 810, 1063, 1141, 1359, 1573, 1798
FauI	CCCGC	1 cut(s)	1900
FbaI	TGATCA	2 cut(s)	69, 1735
FokI	GGATG	3 cut(s)	1418, 1422, 1691
FriOI	GRGCYC	2 cut(s)	194, 521
FspBI	CTAG	6 cut(s)	146, 432, 507, 815, 1151, 1479
GlaI	GCGC	2 cut(s)	31, 33
GsaI	CCCAGC	1 cut(s)	473
GsuI	CTGGAG	3 cut(s)	108, 273, 1191
HaeIII	GGCC	3 cut(s)	192, 519, 610
HapII	CCGG	3 cut(s)	78, 589, 1085
HhaI	GCGC	2 cut(s)	32, 34
Hin1II	CATG	9 cut(s)	250, 726, 797, 814, 1067, 1145, 1363, 1577, 1802
Hin6I	GCGC	2 cut(s)	30, 32
HinP1I	GCGC	2 cut(s)	30, 32
HincII	GTYRAC	1 cut(s)	349
HindII	GTYRAC	1 cut(s)	349
HindIII	AAGCTT	3 cut(s)	215, 698, 966
HpaII	CCGG	3 cut(s)	78, 589, 1085
HphI	GGTGA	8 cut(s)	121, 353, 416, 461, 642, 773, 791, 1930
Hpy166II	GTNNAC	5 cut(s)	153, 349, 361, 604, 1796
Hpy188I	TCNGA	5 cut(s)	69, 139, 241, 928, 1877
Hpy188III	TCNNGA	4 cut(s)	110, 252, 552, 1208
Hpy8I	GTNNAC	5 cut(s)	153, 349, 361, 604, 1796
Hpy99I	CGWCG	1 cut(s)	186
HpyAV	CCTTC	1 cut(s)	476
HpyCH4III	ACNGT	6 cut(s)	449, 616, 737, 892, 1833, 1886
HpyCH4IV	ACGT	2 cut(s)	387, 880
HpyF10VI	GCNNNNNNNGC	4 cut(s)	36, 167, 198, 1898
HpyF3I	CTNAG	6 cut(s)	202, 338, 381, 948, 1244, 1944
HpySE526I	ACGT	2 cut(s)	387, 880
Hsp92II	CATG	9 cut(s)	250, 726, 797, 814, 1067, 1145, 1363, 1577, 1802
HspAI	GCGC	2 cut(s)	30, 32
Ksp22I	TGATCA	2 cut(s)	69, 1735
Kzo9I	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
LmnI	GCTCC	3 cut(s)	715, 757, 1210
Lsp1109I	GCAGC	7 cut(s)	182, 346, 705, 1142, 1406, 1792, 1876
LweI	GCATC	5 cut(s)	43, 565, 739, 1625, 1910
MaeI	CTAG	6 cut(s)	146, 432, 507, 815, 1151, 1479
MaeII	ACGT	2 cut(s)	387, 880
MaeIII	GTNAC	4 cut(s)	127, 761, 1723, 1880
MalI	GATC	8 cut(s)	71, 410, 512, 1030, 1155, 1173, 1286, 1737
MbiI	CCGCTC	1 cut(s)	176
MboI	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
MboII	GAAGA	9 cut(s)	212, 215, 587, 666, 830, 1023, 1260, 1694, 1774
MflI	RGATCY	2 cut(s)	408, 1153
MhlI	GDGCHC	2 cut(s)	194, 521
MlsI	TGGCCA	1 cut(s)	610
MluCI	AATT	8 cut(s)	972, 1044, 1125, 1251, 1303, 1784, 1845, 1870
MluNI	TGGCCA	1 cut(s)	610
MlyI	GAGTC	4 cut(s)	354, 601, 617, 1865
MmeI	TCCRAC	2 cut(s)	162, 1914
Mox20I	TGGCCA	1 cut(s)	610
Mph1103I	ATGCAT	4 cut(s)	315, 580, 1065, 1640
MscI	TGGCCA	1 cut(s)	610
MseI	TTAA	3 cut(s)	1007, 1179, 1262
MslI	CAYNNNNRTG	1 cut(s)	1358
Msp20I	TGGCCA	1 cut(s)	610
MspA1I	CMGCKG	1 cut(s)	1397
MspI	CCGG	3 cut(s)	78, 589, 1085
Mva1269I	GAATGC	3 cut(s)	39, 81, 670
MvnI	CGCG	3 cut(s)	11, 32, 1382
MwoI	GCNNNNNNNGC	4 cut(s)	36, 167, 198, 1898
NdeII	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
NlaIII	CATG	9 cut(s)	250, 726, 797, 814, 1067, 1145, 1363, 1577, 1802
NlaIV	GGNNCC	4 cut(s)	191, 192, 208, 519
NmuCI	GTSAC	2 cut(s)	127, 761
NsiI	ATGCAT	4 cut(s)	315, 580, 1065, 1640
NspI	RCATGY	1 cut(s)	1067
PauI	GCGCGC	1 cut(s)	30
PctI	GAATGC	3 cut(s)	39, 81, 670
PinAI	ACCGGT	1 cut(s)	1084
PleI	GAGTC	4 cut(s)	353, 600, 617, 1864
PpsI	GAGTC	4 cut(s)	353, 600, 617, 1864
Psp1406I	AACGTT	1 cut(s)	387
PspEI	GGTNACC	1 cut(s)	1723
PspFI	CCCAGC	1 cut(s)	469
PspN4I	GGNNCC	4 cut(s)	191, 192, 208, 519
PspOMI	GGGCCC	2 cut(s)	190, 517
PspPI	GGNCC	5 cut(s)	190, 191, 517, 518, 1363
PstNI	CAGNNNCTG	1 cut(s)	992
PsuI	RGATCY	2 cut(s)	408, 1153
PteI	GCGCGC	1 cut(s)	30
PvuII	CAGCTG	1 cut(s)	1397
RsaI	GTAC	2 cut(s)	1083, 1840
RsaNI	GTAC	2 cut(s)	1082, 1839
RseI	CAYNNNNRTG	1 cut(s)	1358
SaqAI	TTAA	3 cut(s)	1007, 1179, 1262
Sau3AI	GATC	8 cut(s)	69, 408, 510, 1028, 1153, 1171, 1284, 1735
Sau96I	GGNCC	5 cut(s)	190, 191, 517, 518, 1363
SchI	GAGTC	4 cut(s)	354, 601, 617, 1865
SduI	GDGCHC	2 cut(s)	194, 521
SfaNI	GCATC	5 cut(s)	43, 565, 739, 1625, 1910
SfcI	CTRYAG	1 cut(s)	156
SinI	GGWCC	1 cut(s)	1363
SmiMI	CAYNNNNRTG	1 cut(s)	1358
Sse9I	AATT	8 cut(s)	972, 1044, 1125, 1251, 1303, 1784, 1845, 1870
SsiI	CCGC	7 cut(s)	9, 57, 174, 287, 1024, 1781, 1907
SspMI	CTAG	6 cut(s)	146, 432, 507, 815, 1151, 1479
StyI	CCWWGG	3 cut(s)	521, 1224, 1618
TaaI	ACNGT	6 cut(s)	449, 616, 737, 892, 1833, 1886
TaiI	ACGT	2 cut(s)	390, 883
TaqI	TCGA	3 cut(s)	90, 298, 849
TasI	AATT	8 cut(s)	972, 1044, 1125, 1251, 1303, 1784, 1845, 1870
TauI	GCSGC	3 cut(s)	176, 290, 1027
Tru1I	TTAA	3 cut(s)	1007, 1179, 1262
Tru9I	TTAA	3 cut(s)	1007, 1179, 1262
TscAI	CASTG	6 cut(s)	611, 621, 742, 984, 1041, 1654
TseFI	GTSAC	2 cut(s)	127, 761
TseI	GCWGC	7 cut(s)	170, 334, 718, 1130, 1394, 1805, 1889
Tsp45I	GTSAC	2 cut(s)	127, 761
TspDTI	ATGAA	6 cut(s)	810, 926, 1287, 1598, 1695, 1815
TspGWI	ACGGA	1 cut(s)	199
TspRI	CASTG	6 cut(s)	611, 621, 742, 984, 1041, 1654
VpaK11BI	GGWCC	1 cut(s)	1363
XapI	RAATTY	3 cut(s)	1044, 1303, 1870
XceI	RCATGY	1 cut(s)	1067
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1357
XspI	CTAG	6 cut(s)	146, 432, 507, 815, 1151, 1479
Zsp2I	ATGCAT	4 cut(s)	315, 580, 1065, 1640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.