Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

4319261 .. 4323216

Gene Structure 3956 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1060200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGTGCGGAATCGCTTTGATCATCTGGGGAATTCGTTTTGATCTGTCGTCCCTGCTTCTCAATCCTACACCTCCACTCCCCAACTCCGACCAACTTGCGTTCGCCATTGACGATCTAAGAAAAGCTTTGCGGAGGAGGGGTCCCGATAGCTTAGGCAGCACGAAACTTCTTCTCCATTCAGCCGTTTCGAATCGTCTCCCGGTGCGGGAAATTGTGTCTTCCATTGAGGGGGTAGTAAAGGAGGAGGCTGGTCGCGAAGGCGATGAAGGCAAATGTTTCTCTTTGGAAAATGATCGAACGCCTCATCTGCATACCCATTTCAGCACCGTTCCCAGTTCGTCTGCTGAGTTGCACTTCCTGGGTGCCACTTTGCAGCTCAGGGGAGTAAGCCCTGTGGTTCAGCCATTGATAGATTCGGATAAGAATATCCTTGTTTTTAATGGTGAAATCTTTGGGGGCATAGACATTGGTAGAGATGAGAACGATGGTGAAGTTCTTTTGCAGTTGCTGGTACAGTGCTGTTCGGGTTCTGTTCCAGATGTTCTTTCTAGGATCAAAGGGCCTTGGGCTATCATCTATTGGCAGGAAAGTTCAAAAACTCTGTGGTTTGCTCGAGATGCATTTGGTCGGCGGAGTCTTCTTGTTCACTGGCCAACACTGGAGGACTCTCGGTTTCTGCTGTCGTCTGTATCACCTTTTTCTTCAAATGAGCGTTCTTTTGATTTTGAAGCTGAAAATGGAACTACCAAGCTGAATTTTTGGGAAGAGCTCCGATGTGGAATATACAGTATATCTATGAATGCTCCAGGTCTTGATGGGATTTTGGTTGGTGAAGTGCAAAAACATGAGTGGACAAATGGTGTACTAGAAGAACTGATAAAATGGGAGAGAACTTATGTCGAACGTAAACCTGAAGACTTACACATTTCCCATTCCAAGACTCTGACAGGGAAACGTGATATGCACTCAGTTAATTCAGGATCTATACAAGCTTCAATTTCTGTGCCAGCACAGGCAGTACTGATTGCTTTAAGGGAATCTGTTAGGCGACGCTCTTCACTGCACACAATTTTTCAGGCAGGTAGACATGATATGGAACAGGAATTTGTACCGGTGGCAGTTCTTTTTTCTGGTGGATTAGATTCTATGATAATTGCAGCCTTGTTGCATGAGTGCCTTGATCCGAGCTATGATATTGATCTACTTAATGTGAGCTTTGATGGTCGGTCTGCTCCTGACAGAATCTCTGCCAGGGCAGGAGTAAGCGAACTGAGAAGAATTGCACCTTCAAGAAAGTGGAAGCTTGTGGAGATTGACGCTGAATTGTCAACATTGACCTTTGAAACAAAGCATGTCATGTCACTCATAAATCCTGCTAACACCTACATGGACCTGAATATAGGAATAGCTTTGTGGCTAGCTGCTGGTGGTGATGGATGGGTATATGAGGAAAATACCAATTATAATGATGAGGATTGTCAATGCGTCAAGTACAAGTCCAAGGCGAGGATTCTTCTTGTTGGTTCAGGTGCAGATGAGCAATGTGCTGGCTATGGTAGGCACAGAACGAAATATAGAAGTGGCGGCTGGCGTGCACTAAACGATGAAATGAAATTGGACATGCAGAGAATTTGGAAAAGAAACCTTGGGAGAGATGATCGGTGCATTGCTGACAATGGCAAGGAGGCAAGGTTTCCGTTTCTGGATGAAGATGTTATAAGAATTTTGCTTGGGTTTCCTCTCTGGGAGGTTGCCAACCTTGATCAACCGTGCGGGATAGGAGATAAGAAGATTCTGAGAGGGGTCGCAGAATTGCTTGGTTTGTATGAAGCTGCTTCTCTGCCTAAAAGAGCTATACAGTTTGGTTCAAGAATTGCAAGGGAATCAAACCGAAAGAATTACGGAAGTAACCGTGCAGCAAATCAAGCATCTGCAGGAAGTGCAGTGATTCACAAACGGTTGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

656

Amino Acids

73.01

Weight (kDa)

6.11

Isoelectric Point (pI)

44.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	124 - 218	8.8e-07	Glutamine amidotransferase domain
GATase_7	PF13537	133 - 229	1.5e-09	Glutamine amidotransferase domain
Asn_synthase	PF00733	527 - 603	3.1e-06	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1464, 1718
Acc36I	ACCTGC	1 cut(s)	1070
AccB1I	GGYRCC	1 cut(s)	362
AccI	GTMKAC	1 cut(s)	1084
AccII	CGCG	1 cut(s)	255
AciI	CCGC	6 cut(s)	6, 130, 205, 631, 1584, 1773
AclWI	GGATC	3 cut(s)	560, 988, 1175
AcoI	YGGCCR	1 cut(s)	650
AcsI	RAATTY	5 cut(s)	30, 754, 1103, 1629, 1722
AcuI	CTGAAG	1 cut(s)	933
AfaI	GTAC	5 cut(s)	513, 864, 1020, 1110, 1493
AfiI	CCNNNNNNNGG	3 cut(s)	205, 228, 1506
AgeI	ACCGGT	1 cut(s)	1111
AgsI	TTSAA	7 cut(s)	594, 705, 728, 996, 1290, 1343, 1869
AjnI	CCWGG	3 cut(s)	357, 805, 1250
Alw21I	GWGCWC	2 cut(s)	771, 1597
Alw26I	GTCTC	1 cut(s)	200
Alw44I	GTGCAC	1 cut(s)	1593
AlwI	GGATC	3 cut(s)	560, 988, 1175
AlwNI	CAGNNNCTG	1 cut(s)	508
Ama87I	CYCGRG	1 cut(s)	612
AoxI	GGCC	2 cut(s)	560, 650
ApaLI	GTGCAC	1 cut(s)	1593
ApeKI	GCWGC	6 cut(s)	156, 373, 1157, 1421, 1832, 1916
ApoI	RAATTY	5 cut(s)	30, 754, 1103, 1629, 1722
AsiGI	ACCGGT	1 cut(s)	1111
AspS9I	GGNCC	3 cut(s)	140, 560, 1390
AsuC2I	CCSGG	1 cut(s)	200
AsuHPI	GGTGA	5 cut(s)	455, 500, 684, 842, 1442
AsuII	TTCGAA	1 cut(s)	188
AsuNHI	GCTAGC	1 cut(s)	1417
AvaI	CYCGRG	1 cut(s)	612
AvaII	GGWCC	2 cut(s)	140, 1390
BaeGI	GKGCMC	1 cut(s)	1597
BalI	TGGCCA	1 cut(s)	652
BanI	GGYRCC	1 cut(s)	362
BanII	GRGCYC	1 cut(s)	771
BbsI	GAAGAC	3 cut(s)	210, 629, 921
Bbv12I	GWGCWC	2 cut(s)	771, 1597
BbvI	GCAGC	6 cut(s)	168, 385, 1169, 1408, 1819, 1928
BccI	CCATC	5 cut(s)	479, 809, 1214, 1427, 1431
BceAI	ACGGC	1 cut(s)	167
BcgI	CGANNNNNNTGC	2 cut(s)	77, 111
BciT130I	CCWGG	3 cut(s)	359, 807, 1252
BclI	TGATCA	2 cut(s)	18, 1762
BcnI	CCSGG	1 cut(s)	200
BcoDI	GTCTC	1 cut(s)	200
BfaI	CTAG	3 cut(s)	549, 866, 1418
BfmI	CTRYAG	1 cut(s)	1932
BfuAI	ACCTGC	1 cut(s)	1070
BisI	GCNGC	7 cut(s)	157, 374, 1158, 1422, 1585, 1833, 1917
BlsI	GCNGC	7 cut(s)	158, 375, 1159, 1423, 1586, 1834, 1918
BmcAI	AGTACT	1 cut(s)	1020
Bme1390I	CCNGG	4 cut(s)	200, 359, 807, 1252
Bme18I	GGWCC	2 cut(s)	140, 1390
BmeT110I	CYCGRG	1 cut(s)	612
BmgT120I	GGNCC	3 cut(s)	140, 560, 1390
BmiI	GGNNCC	3 cut(s)	141, 142, 364
BmrFI	CCNGG	4 cut(s)	200, 359, 807, 1252
BmrI	ACTGGG	1 cut(s)	327
BmsI	GCATC	2 cut(s)	607, 1937
BmtI	GCTAGC	1 cut(s)	1421
BmuI	ACTGGG	1 cut(s)	327
BpiI	GAAGAC	3 cut(s)	210, 629, 921
BpmI	CTGGAG	2 cut(s)	680, 789
Bpu10I	CCTNAGC	2 cut(s)	151, 377
Bpu14I	TTCGAA	1 cut(s)	188
BpuMI	CCSGG	1 cut(s)	200
BsaBI	GATNNNNATC	1 cut(s)	1197
BsaJI	CCNNGG	5 cut(s)	358, 563, 1251, 1500, 1645
BsaWI	WCCGGW	1 cut(s)	1111
BsaXI	ACNNNNNCTCC	8 cut(s)	60, 90, 124, 154, 156, 186, 1676, 1706
Bsc4I	CCNNNNNNNGG	3 cut(s)	205, 228, 1506
Bse118I	RCCGGY	1 cut(s)	1111
Bse1I	ACTGG	3 cut(s)	333, 653, 663
Bse3DI	GCAATG	2 cut(s)	1547, 1665
Bse8I	GATNNNNATC	1 cut(s)	1197
BseBI	CCWGG	3 cut(s)	359, 807, 1252
BseDI	CCNNGG	5 cut(s)	358, 563, 1251, 1500, 1645
BseGI	GGATG	2 cut(s)	1442, 1711
BseJI	GATNNNNATC	1 cut(s)	1197
BseLI	CCNNNNNNNGG	3 cut(s)	205, 228, 1506
BseMI	GCAATG	2 cut(s)	1547, 1665
BseMII	CTCAG	5 cut(s)	336, 391, 981, 1262, 1787
BseNI	ACTGG	3 cut(s)	333, 653, 663
BseRI	GAGGAG	2 cut(s)	148, 257
BseSI	GKGCMC	1 cut(s)	1597
BseXI	GCAGC	6 cut(s)	168, 385, 1169, 1408, 1819, 1928
BsgI	GTGCAG	4 cut(s)	1046, 1551, 1935, 1962
Bsh1236I	CGCG	1 cut(s)	255
BshFI	GGCC	2 cut(s)	562, 652
BshNI	GGYRCC	1 cut(s)	362
BshTI	ACCGGT	1 cut(s)	1111
BsiHKAI	GWGCWC	2 cut(s)	771, 1597
BsiHKCI	CYCGRG	1 cut(s)	612
BsiSI	CCGG	2 cut(s)	200, 1112
BslFI	GGGAC	2 cut(s)	34, 126
BslI	CCNNNNNNNGG	3 cut(s)	205, 228, 1506
BsmAI	GTCTC	1 cut(s)	200
BsmBI	CGTCTC	1 cut(s)	200
BsmFI	GGGAC	2 cut(s)	34, 126
BsmI	GAATGC	1 cut(s)	805
BsnI	GGCC	2 cut(s)	562, 652
BsoBI	CYCGRG	1 cut(s)	612
Bsp119I	TTCGAA	1 cut(s)	188
Bsp1286I	GDGCHC	2 cut(s)	771, 1597
Bsp68I	TCGCGA	1 cut(s)	255
BspACI	CCGC	6 cut(s)	6, 130, 205, 631, 1584, 1773
BspANI	GGCC	2 cut(s)	562, 652
BspCNI	CTCAG	5 cut(s)	337, 390, 980, 1263, 1788
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	3 cut(s)	141, 142, 364
BspMAI	CTGCAG	1 cut(s)	1936
BspMI	ACCTGC	1 cut(s)	1070
BspOI	GCTAGC	1 cut(s)	1421
BspPI	GGATC	3 cut(s)	560, 988, 1175
BspQI	GCTCTTC	2 cut(s)	759, 1060
BspT104I	TTCGAA	1 cut(s)	188
BspT107I	GGYRCC	1 cut(s)	362
BsrDI	GCAATG	2 cut(s)	1547, 1665
BsrFI	RCCGGY	1 cut(s)	1111
BsrI	ACTGG	3 cut(s)	333, 653, 663
BssAI	RCCGGY	1 cut(s)	1111
BssECI	CCNNGG	5 cut(s)	358, 563, 1251, 1500, 1645
BssT1I	CCWWGG	3 cut(s)	563, 1500, 1645
Bst2UI	CCWGG	3 cut(s)	359, 807, 1252
Bst4CI	ACNGT	7 cut(s)	328, 516, 788, 1770, 1860, 1913, 1959
Bst6I	CTCTTC	2 cut(s)	759, 1060
BstAPI	GCANNNNNTGC	1 cut(s)	1940
BstBI	TTCGAA	1 cut(s)	188
BstC8I	GCNNGC	5 cut(s)	1008, 1419, 1549, 1589, 1593
BstDEI	CTNAG	7 cut(s)	116, 151, 345, 377, 967, 1271, 1796
BstF5I	GGATG	2 cut(s)	1442, 1711
BstFNI	CGCG	1 cut(s)	255
BstMAI	GTCTC	1 cut(s)	200
BstMWI	GCNNNNNNNGC	6 cut(s)	156, 267, 307, 617, 1925, 1940
BstNI	CCWGG	3 cut(s)	359, 807, 1252
BstNSI	RCATGY	2 cut(s)	1355, 1624
BstSCI	CCNGG	4 cut(s)	198, 357, 805, 1250
BstSFI	CTRYAG	1 cut(s)	1932
BstSLI	GKGCMC	1 cut(s)	1597
BstUI	CGCG	1 cut(s)	255
BstV1I	GCAGC	6 cut(s)	168, 385, 1169, 1408, 1819, 1928
BstV2I	GAAGAC	3 cut(s)	210, 629, 921
BstX2I	RGATCY	1 cut(s)	980
BstYI	RGATCY	1 cut(s)	980
BsuRI	GGCC	2 cut(s)	562, 652
BtgZI	GCGATG	1 cut(s)	276
BtsCI	GGATG	2 cut(s)	1442, 1711
BtsI	GCAGTG	2 cut(s)	1058, 1950
BtsIMutI	CAGTG	5 cut(s)	521, 646, 656, 1058, 1950
BtuMI	TCGCGA	1 cut(s)	255
BveI	ACCTGC	1 cut(s)	1070
Cac8I	GCNNGC	5 cut(s)	1008, 1419, 1549, 1589, 1593
CaiI	CAGNNNCTG	1 cut(s)	508
Cfr10I	RCCGGY	1 cut(s)	1111
Cfr13I	GGNCC	3 cut(s)	140, 560, 1390
CseI	GACGC	3 cut(s)	1059, 1325, 1474
Csp6I	GTAC	5 cut(s)	512, 863, 1019, 1109, 1492
CspAI	ACCGGT	1 cut(s)	1111
CviAII	CATG	7 cut(s)	845, 1088, 1169, 1352, 1357, 1387, 1621
CviQI	GTAC	5 cut(s)	512, 863, 1019, 1109, 1492
DdeI	CTNAG	7 cut(s)	116, 151, 345, 377, 967, 1271, 1796
EaeI	YGGCCR	1 cut(s)	650
Eam1104I	CTCTTC	2 cut(s)	759, 1060
EarI	CTCTTC	2 cut(s)	759, 1060
EciI	GGCGGA	1 cut(s)	646
Ecl136II	GAGCTC	1 cut(s)	769
Eco130I	CCWWGG	3 cut(s)	563, 1500, 1645
Eco24I	GRGCYC	1 cut(s)	771
Eco47I	GGWCC	2 cut(s)	140, 1390
Eco53kI	GAGCTC	1 cut(s)	769
Eco57I	CTGAAG	1 cut(s)	933
Eco88I	CYCGRG	1 cut(s)	612
EcoICRI	GAGCTC	1 cut(s)	769
EcoO109I	RGGNCCY	2 cut(s)	140, 560
EcoRI	GAATTC	1 cut(s)	30
EcoRII	CCWGG	3 cut(s)	357, 805, 1250
EcoT14I	CCWWGG	3 cut(s)	563, 1500, 1645
EcoT22I	ATGCAT	1 cut(s)	622
EcoT38I	GRGCYC	1 cut(s)	771
ErhI	CCWWGG	3 cut(s)	563, 1500, 1645
Esp3I	CGTCTC	1 cut(s)	200
FaeI	CATG	7 cut(s)	848, 1091, 1172, 1355, 1360, 1390, 1624
FalI	AAGNNNNNCTT	2 cut(s)	109, 141
FaqI	GGGAC	2 cut(s)	34, 126
FatI	CATG	7 cut(s)	844, 1087, 1168, 1351, 1356, 1386, 1620
FauI	CCCGC	2 cut(s)	198, 1766
FbaI	TGATCA	2 cut(s)	18, 1762
FblI	GTMKAC	1 cut(s)	1084
Fnu4HI	GCNGC	7 cut(s)	157, 374, 1158, 1422, 1585, 1833, 1917
FokI	GGATG	2 cut(s)	1449, 1718
FriOI	GRGCYC	1 cut(s)	771
Fsp4HI	GCNGC	7 cut(s)	157, 374, 1158, 1422, 1585, 1833, 1917
FspBI	CTAG	3 cut(s)	549, 866, 1418
GluI	GCNGC	7 cut(s)	157, 374, 1158, 1422, 1585, 1833, 1917
GsuI	CTGGAG	2 cut(s)	680, 789
HaeIII	GGCC	2 cut(s)	562, 652
HapII	CCGG	2 cut(s)	200, 1112
HgaI	GACGC	3 cut(s)	1059, 1325, 1474
Hin1II	CATG	7 cut(s)	848, 1091, 1172, 1355, 1360, 1390, 1624
HincII	GTYRAC	1 cut(s)	1329
HindII	GTYRAC	1 cut(s)	1329
HindIII	AAGCTT	3 cut(s)	123, 990, 1301
HpaII	CCGG	2 cut(s)	200, 1112
HphI	GGTGA	5 cut(s)	455, 500, 684, 842, 1442
Hpy166II	GTNNAC	7 cut(s)	646, 852, 863, 908, 1085, 1329, 1595
Hpy188I	TCNGA	6 cut(s)	88, 418, 773, 945, 1185, 1797
Hpy8I	GTNNAC	7 cut(s)	646, 852, 863, 908, 1085, 1329, 1595
Hpy99I	CGWCG	1 cut(s)	1053
HpyAV	CCTTC	3 cut(s)	251, 260, 1296
HpyCH4III	ACNGT	7 cut(s)	328, 516, 788, 1770, 1860, 1913, 1959
HpyCH4IV	ACGT	2 cut(s)	904, 955
HpyF10VI	GCNNNNNNNGC	6 cut(s)	156, 267, 307, 617, 1925, 1940
HpyF3I	CTNAG	7 cut(s)	116, 151, 345, 377, 967, 1271, 1796
HpySE526I	ACGT	2 cut(s)	904, 955
Hsp92II	CATG	7 cut(s)	848, 1091, 1172, 1355, 1360, 1390, 1624
KflI	GGGWCCC	1 cut(s)	140
Ksp22I	TGATCA	2 cut(s)	18, 1762
LguI	GCTCTTC	2 cut(s)	759, 1060
LmnI	GCTCC	3 cut(s)	774, 808, 1237
Lsp1109I	GCAGC	6 cut(s)	168, 385, 1169, 1408, 1819, 1928
LweI	GCATC	2 cut(s)	607, 1937
MaeI	CTAG	3 cut(s)	549, 866, 1418
MaeII	ACGT	2 cut(s)	904, 955
MaeIII	GTNAC	2 cut(s)	1359, 1907
MflI	RGATCY	1 cut(s)	980
MhlI	GDGCHC	2 cut(s)	771, 1597
MlsI	TGGCCA	1 cut(s)	652
MluNI	TGGCCA	1 cut(s)	652
MlyI	GAGTC	3 cut(s)	643, 659, 934
MmeI	TCCRAC	2 cut(s)	111, 1941
Mox20I	TGGCCA	1 cut(s)	652
Mph1103I	ATGCAT	1 cut(s)	622
MscI	TGGCCA	1 cut(s)	652
MseI	TTAA	4 cut(s)	438, 972, 1031, 1206
MslI	CAYNNNNRTG	1 cut(s)	1385
Msp20I	TGGCCA	1 cut(s)	652
MspI	CCGG	2 cut(s)	200, 1112
MspR9I	CCNGG	4 cut(s)	200, 359, 807, 1252
Mva1269I	GAATGC	1 cut(s)	805
MvaI	CCWGG	3 cut(s)	359, 807, 1252
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	6 cut(s)	156, 267, 307, 617, 1925, 1940
NciI	CCSGG	1 cut(s)	200
NheI	GCTAGC	1 cut(s)	1417
NlaIII	CATG	7 cut(s)	848, 1091, 1172, 1355, 1360, 1390, 1624
NlaIV	GGNNCC	3 cut(s)	141, 142, 364
NmuCI	GTSAC	1 cut(s)	1359
NruI	TCGCGA	1 cut(s)	255
NsiI	ATGCAT	1 cut(s)	622
NspI	RCATGY	2 cut(s)	1355, 1624
NspV	TTCGAA	1 cut(s)	188
PaeR7I	CTCGAG	1 cut(s)	612
PciSI	GCTCTTC	2 cut(s)	759, 1060
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PctI	GAATGC	1 cut(s)	805
PinAI	ACCGGT	1 cut(s)	1111
PkrI	GCNGC	7 cut(s)	158, 375, 1159, 1423, 1586, 1834, 1918
PleI	GAGTC	3 cut(s)	642, 659, 934
PpsI	GAGTC	3 cut(s)	642, 659, 934
PpuMI	RGGWCCY	1 cut(s)	140
PsiI	TTATAA	2 cut(s)	1464, 1718
Psp124BI	GAGCTC	1 cut(s)	771
Psp5II	RGGWCCY	1 cut(s)	140
Psp6I	CCWGG	3 cut(s)	357, 805, 1250
PspGI	CCWGG	3 cut(s)	357, 805, 1250
PspN4I	GGNNCC	3 cut(s)	141, 142, 364
PspPI	GGNCC	3 cut(s)	140, 560, 1390
PspPPI	RGGWCCY	1 cut(s)	140
PstI	CTGCAG	1 cut(s)	1936
PstNI	CAGNNNCTG	1 cut(s)	508
PsuI	RGATCY	1 cut(s)	980
RruI	TCGCGA	1 cut(s)	255
RsaI	GTAC	5 cut(s)	513, 864, 1020, 1110, 1493
RsaNI	GTAC	5 cut(s)	512, 863, 1019, 1109, 1492
RseI	CAYNNNNRTG	1 cut(s)	1385
SacI	GAGCTC	1 cut(s)	771
SapI	GCTCTTC	2 cut(s)	759, 1060
SaqAI	TTAA	4 cut(s)	438, 972, 1031, 1206
SatI	GCNGC	7 cut(s)	157, 374, 1158, 1422, 1585, 1833, 1917
Sau96I	GGNCC	3 cut(s)	140, 560, 1390
ScaI	AGTACT	1 cut(s)	1020
SchI	GAGTC	3 cut(s)	643, 659, 934
ScrFI	CCNGG	4 cut(s)	200, 359, 807, 1252
SduI	GDGCHC	2 cut(s)	771, 1597
SfaNI	GCATC	2 cut(s)	607, 1937
SfcI	CTRYAG	1 cut(s)	1932
Sfr274I	CTCGAG	1 cut(s)	612
SfuI	TTCGAA	1 cut(s)	188
SinI	GGWCC	2 cut(s)	140, 1390
SlaI	CTCGAG	1 cut(s)	612
SmiMI	CAYNNNNRTG	1 cut(s)	1385
SmlI	CTYRAG	1 cut(s)	612
SmoI	CTYRAG	1 cut(s)	612
SsiI	CCGC	6 cut(s)	6, 130, 205, 631, 1584, 1773
SspMI	CTAG	3 cut(s)	549, 866, 1418
SstI	GAGCTC	1 cut(s)	771
StyD4I	CCNGG	4 cut(s)	198, 357, 805, 1250
StyI	CCWWGG	3 cut(s)	563, 1500, 1645
TaaI	ACNGT	7 cut(s)	328, 516, 788, 1770, 1860, 1913, 1959
TaiI	ACGT	2 cut(s)	907, 958
TaqI	TCGA	4 cut(s)	188, 295, 613, 900
TaqII	GACCGA	1 cut(s)	1215
TatI	WGTACW	3 cut(s)	862, 1018, 1491
TauI	GCSGC	1 cut(s)	1587
Tru1I	TTAA	4 cut(s)	438, 972, 1031, 1206
Tru9I	TTAA	4 cut(s)	438, 972, 1031, 1206
TscAI	CASTG	5 cut(s)	521, 653, 663, 1065, 1950
TseFI	GTSAC	1 cut(s)	1359
TseI	GCWGC	6 cut(s)	156, 373, 1157, 1421, 1832, 1916
Tsp45I	GTSAC	1 cut(s)	1359
TspDTI	ATGAA	6 cut(s)	279, 812, 1620, 1625, 1722, 1842
TspGWI	ACGGA	2 cut(s)	1686, 1917
TspRI	CASTG	5 cut(s)	521, 653, 663, 1065, 1950
VneI	GTGCAC	1 cut(s)	1593
VpaK11BI	GGWCC	2 cut(s)	140, 1390
XapI	RAATTY	5 cut(s)	30, 754, 1103, 1629, 1722
XceI	RCATGY	2 cut(s)	1355, 1624
XhoI	CTCGAG	1 cut(s)	612
XmiI	GTMKAC	1 cut(s)	1084
XspI	CTAG	3 cut(s)	549, 866, 1418
ZrmI	AGTACT	1 cut(s)	1020
Zsp2I	ATGCAT	1 cut(s)	622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.