Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Forward (+)

6673776 .. 6677845

Gene Structure 4070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006385

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGTGCGGAATCGCTCTGATCATCTCCGGCATTCGCTTCGACTTATCGTCTCTGCCTCTCGACACCACACCTCCAGTCACCAACTCCGACCAACTAGAGTTCACTGTAGCCGACCTCGCAGCCGCTCTGCGACGGAGGGGCCCAGATAGCTTAGGCACCAAGAAGCTTCTTCTTCACTCATCAGGCTCCGAAATCGTGTCTGGAGTAGAGGAGGAGTTCAGCAACTGCTTCTCTGCGGCAAATGGTCGAACACCCTGTATGCACTTTTTTGGTGCTACTTTGCAGCTCAGGGGAGTCAACCCAGTAGTTCACCCATTGATAGATTCGTCTAACAACGTTCTTGTCTATAATGGTGAGATCTTTGGGGGGATTGAGGTTGCTAGTGAGGAGAATGATGGTGAAGTTCTAATGGGGTTGCTGGGAGGGTGTGAAGGTTGTGTGCCGAATGTTGTTTCTAGGATCAAGGGGCCTTGGGCTATCATCTATTGGCAGGAAAGTTCAAGAACATTATGGTTTGGGAGAGATGCATTTGGTCGCCGGAGTCTTCTTGTTCATTGGCCGACAGTGGAGGACTCTCGGTTTCTGTTATCATCAGTATCACCCGTTGCTTCAAATGAGCATTCTTCTGCTGAAAATGGAACAACCAAGCTTGGTTTTTGGGAGGAGCTGCCATGTGGAATATACAGTGTATCCATAGATGCTCCCGATGGTGACGAGAGTTTGGTTGGTGAAGTGAAAAAGCATGAATGGACTAATGCCATGCTACAAGAACTGATAAAATGGGAGAGAACATCTGTCGAACCCAAACCTGAAGAGTTGCATATTTCACGTTGTGAGACTGTAAGAGGGCAACACGACATGAACTCACTTACTTCAGATATAGTGTCAATGCCATCTAAGTCAGGCAATATACAAGCTTCAATTTCAGTGCCAGCAGAGGCTGTGCTGATTGCTTTAAGGGAATCAGTTTTGCGGCGATCTTCACTGCACCGAATTTTTCAGGCAAGTATGCATGATGTGGAAAAGAATCTTGTACCGGTGGCAGTTCTTTTTTCTGGTGGATTGGATTCTATGATAATTGCAGCATTGTTGCATGAGTGCCTAGATCCAAGCTATGAGATTGATCTACTTAATGTGAGCTTCGATCGTCAGTCTGCTCCAGATAGAATCTCTGCCAAGGCAGGAGTGAGCGAACTGAGAAGAATTGCACCTTTAAGAAAGTGGAAACTTGTGGAGATCGATGCTGATTTATCAAATTTGAACTTTGAAACAAAGCGTGTATTGTCTCTTATAAACCCTGCCAACACCTACATGGACCTAAATATAGGAATTGCGTTATGGCTGGCAGCTGGTGGGGATGGATGGGTTTATGAGGAAATTAGCAAATATAATGATGAGAATCGTCAAGGTGTTAAATACAAGTCAAAGGCTAGGATTGTCCTTGTTGGTTCGGGTGCAGATGAGCAATGTGCTGGCTATGGTAGGCACAGAACAAAATATAGAAGTGGAAGTTGGCTGCGACTGCATGAGGAAATGAAACTGGATATGCGAAGAATATGGAAAAGAAATCTTGGTAGAGATGATAGATGCATTGCTGACACTGGCAAGGAGGCAAGGTTTCCTTTCTTGGATGAAGATGTTATACGAACTTTATTGGGGATTCCTCTCTGGGAGGTTACCAACCTTGATCAACCAAGTGGAATAGGAGATAAGAAGATTTTGAGAGAGGTTGCGGAATTGCTTGGTTTACATGAAGCTGCTACTCTGCCTAAACGAGCTATACAGTTTGGTACAAGAATTGCGAGGGAGTCCAATAGAAAGAATTTCGGAAGTAACCGTGCTGCAAATCAGGCATCTGCGGGAAGTGTGGTGATTCACAAGCAGTTGGACACGGCTTCGTGGGATTGTTTGATGCAAGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

641

Amino Acids

70.75

Weight (kDa)

5.77

Isoelectric Point (pI)

44.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	105 - 198	1.6e-07	Glutamine amidotransferase domain
Asn_synthase	PF00733	502 - 594	2.8e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1292
AccB1I	GGYRCC	1 cut(s)	155
AccBSI	CCGCTC	1 cut(s)	125
AciI	CCGC	6 cut(s)	6, 123, 236, 973, 1733, 1859
AclI	AACGTT	1 cut(s)	336
AclWI	GGATC	2 cut(s)	467, 1100
AcoI	YGGCCR	1 cut(s)	557
AcsI	RAATTY	3 cut(s)	993, 1255, 1822
AcuI	CTGAAG	2 cut(s)	831, 858
AdeI	CACNNNGTG	1 cut(s)	833
AfaI	GTAC	2 cut(s)	1035, 1792
AgeI	ACCGGT	1 cut(s)	1036
AgsI	TTSAA	5 cut(s)	501, 612, 921, 1261, 1268
AhdI	GACNNNNNGTC	1 cut(s)	46
AjuI	GAANNNNNNNTTGG	2 cut(s)	1495, 1527
Alw26I	GTCTC	3 cut(s)	54, 830, 1290
AlwI	GGATC	2 cut(s)	467, 1100
AlwNI	CAGNNNCTG	2 cut(s)	225, 941
AoxI	GGCC	3 cut(s)	139, 467, 557
ApaI	GGGCCC	1 cut(s)	143
ApeKI	GCWGC	8 cut(s)	119, 283, 667, 1082, 1346, 1516, 1757, 1841
ApoI	RAATTY	3 cut(s)	993, 1255, 1822
AsiGI	ACCGGT	1 cut(s)	1036
AspS9I	GGNCC	4 cut(s)	139, 140, 467, 1315
AsuHPI	GGTGA	8 cut(s)	70, 302, 365, 410, 591, 722, 740, 1882
AvaII	GGWCC	1 cut(s)	1315
BaeGI	GKGCMC	1 cut(s)	143
BanI	GGYRCC	1 cut(s)	155
BanII	GRGCYC	1 cut(s)	143
BarI	GAAGNNNNNNTAC	2 cut(s)	1626, 1658
BbsI	GAAGAC	1 cut(s)	536
BbvI	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
BccI	CCATC	5 cut(s)	389, 701, 901, 1352, 1356
BceAI	ACGGC	1 cut(s)	1908
BcgI	CGANNNNNNTGC	2 cut(s)	19, 53
BciVI	GTATCC	1 cut(s)	700
BclI	TGATCA	2 cut(s)	18, 1687
BcoDI	GTCTC	3 cut(s)	54, 830, 1290
BfaI	CTAG	6 cut(s)	95, 381, 456, 1103, 1431, 1921
BfmI	CTRYAG	1 cut(s)	105
BfuI	GTATCC	1 cut(s)	700
BglII	AGATCT	1 cut(s)	357
Bme18I	GGWCC	1 cut(s)	1315
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgT120I	GGNCC	4 cut(s)	139, 140, 467, 1315
BmiI	GGNNCC	5 cut(s)	140, 141, 157, 187, 468
BmrI	ACTGGG	1 cut(s)	296
BmsI	GCATC	6 cut(s)	514, 688, 1231, 1577, 1862, 1902
BmuI	ACTGGG	1 cut(s)	296
BpiI	GAAGAC	1 cut(s)	536
BpmI	CTGGAG	3 cut(s)	57, 222, 1143
Bpu10I	CCTNAGC	2 cut(s)	151, 287
Bsa29I	ATCGAT	1 cut(s)	1239
BsaBI	GATNNNNATC	1 cut(s)	1398
BsaJI	CCNNGG	2 cut(s)	470, 1176
BsaWI	WCCGGW	1 cut(s)	1036
BsaXI	ACNNNNNCTCC	4 cut(s)	55, 85, 1601, 1631
Bse118I	RCCGGY	1 cut(s)	1036
Bse1I	ACTGG	4 cut(s)	74, 302, 1545, 1606
Bse3DI	GCAATG	2 cut(s)	1472, 1590
Bse8I	GATNNNNATC	1 cut(s)	1398
BseCI	ATCGAT	1 cut(s)	1239
BseDI	CCNNGG	2 cut(s)	470, 1176
BseGI	GGATG	3 cut(s)	1363, 1367, 1636
BseJI	GATNNNNATC	1 cut(s)	1398
BseMI	GCAATG	2 cut(s)	1472, 1590
BseMII	CTCAG	2 cut(s)	301, 1187
BseNI	ACTGG	4 cut(s)	74, 302, 1545, 1606
BseRI	GAGGAG	4 cut(s)	224, 227, 401, 677
BseSI	GKGCMC	1 cut(s)	143
BseXI	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
BseYI	CCCAGC	1 cut(s)	418
BsgI	GTGCAG	2 cut(s)	971, 1476
Bsh1285I	CGRYCG	1 cut(s)	1147
BshFI	GGCC	3 cut(s)	141, 469, 559
BshNI	GGYRCC	1 cut(s)	155
BshTI	ACCGGT	1 cut(s)	1036
BshVI	ATCGAT	1 cut(s)	1239
BsiEI	CGRYCG	1 cut(s)	1147
BsiSI	CCGG	3 cut(s)	27, 538, 1037
BsmAI	GTCTC	3 cut(s)	54, 830, 1290
BsmBI	CGTCTC	1 cut(s)	54
BsmI	GAATGC	2 cut(s)	30, 619
BsnI	GGCC	3 cut(s)	141, 469, 559
Bsp120I	GGGCCC	1 cut(s)	139
Bsp1286I	GDGCHC	1 cut(s)	143
Bsp143I	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
BspACI	CCGC	6 cut(s)	6, 123, 236, 973, 1733, 1859
BspANI	GGCC	3 cut(s)	141, 469, 559
BspCNI	CTCAG	2 cut(s)	300, 1188
BspDI	ATCGAT	1 cut(s)	1239
BspLI	GGNNCC	5 cut(s)	140, 141, 157, 187, 468
BspPI	GGATC	2 cut(s)	467, 1100
BspT107I	GGYRCC	1 cut(s)	155
BsrBI	CCGCTC	1 cut(s)	125
BsrDI	GCAATG	2 cut(s)	1472, 1590
BsrFI	RCCGGY	1 cut(s)	1036
BsrI	ACTGG	4 cut(s)	74, 302, 1545, 1606
BssAI	RCCGGY	1 cut(s)	1036
BssECI	CCNNGG	2 cut(s)	470, 1176
BssMI	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
BssT1I	CCWWGG	2 cut(s)	470, 1176
Bst4CI	ACNGT	6 cut(s)	106, 565, 686, 841, 1785, 1838
Bst6I	CTCTTC	1 cut(s)	807
BstC8I	GCNNGC	3 cut(s)	933, 1344, 1474
BstDEI	CTNAG	4 cut(s)	151, 287, 897, 1196
BstEII	GGTNACC	1 cut(s)	1675
BstF5I	GGATG	3 cut(s)	1363, 1367, 1636
BstKTI	GATC	9 cut(s)	21, 360, 462, 980, 1108, 1126, 1147, 1239, 1690
BstMAI	GTCTC	3 cut(s)	54, 830, 1290
BstMBI	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
BstMCI	CGRYCG	1 cut(s)	1147
BstMWI	GCNNNNNNNGC	4 cut(s)	116, 147, 1522, 1850
BstPI	GGTNACC	1 cut(s)	1675
BstSFI	CTRYAG	1 cut(s)	105
BstSLI	GKGCMC	1 cut(s)	143
BstV1I	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
BstV2I	GAAGAC	1 cut(s)	536
BstX2I	RGATCY	2 cut(s)	357, 1105
BstYI	RGATCY	2 cut(s)	357, 1105
Bsu15I	ATCGAT	1 cut(s)	1239
BsuI	GTATCC	1 cut(s)	700
BsuRI	GGCC	3 cut(s)	141, 469, 559
BsuTUI	ATCGAT	1 cut(s)	1239
BtsCI	GGATG	3 cut(s)	1363, 1367, 1636
BtsI	GCAGTG	1 cut(s)	983
BtsIMutI	CAGTG	6 cut(s)	102, 570, 691, 933, 983, 1599
Cac8I	GCNNGC	3 cut(s)	933, 1344, 1474
CaiI	CAGNNNCTG	2 cut(s)	225, 941
Cfr10I	RCCGGY	1 cut(s)	1036
Cfr13I	GGNCC	4 cut(s)	139, 140, 467, 1315
ClaI	ATCGAT	1 cut(s)	1239
Csp6I	GTAC	2 cut(s)	1034, 1791
CspAI	ACCGGT	1 cut(s)	1036
CviAII	CATG	9 cut(s)	672, 743, 760, 859, 1013, 1094, 1312, 1526, 1751
CviQI	GTAC	2 cut(s)	1034, 1791
DdeI	CTNAG	4 cut(s)	151, 287, 897, 1196
DpnI	GATC	9 cut(s)	20, 359, 461, 979, 1107, 1125, 1146, 1238, 1689
DpnII	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
DraIII	CACNNNGTG	1 cut(s)	833
DriI	GACNNNNNGTC	1 cut(s)	46
EaeI	YGGCCR	1 cut(s)	557
Eam1104I	CTCTTC	1 cut(s)	807
Eam1105I	GACNNNNNGTC	1 cut(s)	46
EarI	CTCTTC	1 cut(s)	807
Eco130I	CCWWGG	2 cut(s)	470, 1176
Eco24I	GRGCYC	1 cut(s)	143
Eco47I	GGWCC	1 cut(s)	1315
Eco57I	CTGAAG	2 cut(s)	831, 858
Eco91I	GGTNACC	1 cut(s)	1675
EcoO109I	RGGNCCY	2 cut(s)	139, 467
EcoO65I	GGTNACC	1 cut(s)	1675
EcoT14I	CCWWGG	2 cut(s)	470, 1176
EcoT22I	ATGCAT	3 cut(s)	529, 1014, 1592
EcoT38I	GRGCYC	1 cut(s)	143
ErhI	CCWWGG	2 cut(s)	470, 1176
Esp3I	CGTCTC	1 cut(s)	54
FaeI	CATG	9 cut(s)	675, 746, 763, 862, 1016, 1097, 1315, 1529, 1754
FalI	AAGNNNNNCTT	2 cut(s)	1606, 1638
FatI	CATG	9 cut(s)	671, 742, 759, 858, 1012, 1093, 1311, 1525, 1750
FauI	CCCGC	1 cut(s)	1852
FbaI	TGATCA	2 cut(s)	18, 1687
FokI	GGATG	3 cut(s)	1370, 1374, 1643
FriOI	GRGCYC	1 cut(s)	143
FspBI	CTAG	6 cut(s)	95, 381, 456, 1103, 1431, 1921
GsaI	CCCAGC	1 cut(s)	422
GsuI	CTGGAG	3 cut(s)	57, 222, 1143
HaeIII	GGCC	3 cut(s)	141, 469, 559
HapII	CCGG	3 cut(s)	27, 538, 1037
Hin1II	CATG	9 cut(s)	675, 746, 763, 862, 1016, 1097, 1315, 1529, 1754
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HindIII	AAGCTT	3 cut(s)	164, 647, 915
HpaII	CCGG	3 cut(s)	27, 538, 1037
HphI	GGTGA	8 cut(s)	70, 302, 365, 410, 591, 722, 740, 1882
Hpy166II	GTNNAC	4 cut(s)	102, 298, 310, 1748
Hpy188I	TCNGA	5 cut(s)	18, 88, 190, 877, 1829
Hpy188III	TCNNGA	5 cut(s)	59, 201, 501, 704, 1160
Hpy8I	GTNNAC	4 cut(s)	102, 298, 310, 1748
Hpy99I	CGWCG	1 cut(s)	135
HpyAV	CCTTC	1 cut(s)	425
HpyCH4III	ACNGT	6 cut(s)	106, 565, 686, 841, 1785, 1838
HpyCH4IV	ACGT	2 cut(s)	336, 829
HpyF10VI	GCNNNNNNNGC	4 cut(s)	116, 147, 1522, 1850
HpyF3I	CTNAG	4 cut(s)	151, 287, 897, 1196
HpySE526I	ACGT	2 cut(s)	336, 829
Hsp92II	CATG	9 cut(s)	675, 746, 763, 862, 1016, 1097, 1315, 1529, 1754
Ksp22I	TGATCA	2 cut(s)	18, 1687
Kzo9I	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
LmnI	GCTCC	4 cut(s)	191, 664, 706, 1162
Lsp1109I	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
LweI	GCATC	6 cut(s)	514, 688, 1231, 1577, 1862, 1902
MaeI	CTAG	6 cut(s)	95, 381, 456, 1103, 1431, 1921
MaeII	ACGT	2 cut(s)	336, 829
MaeIII	GTNAC	4 cut(s)	76, 710, 1675, 1832
MalI	GATC	9 cut(s)	20, 359, 461, 979, 1107, 1125, 1146, 1238, 1689
MbiI	CCGCTC	1 cut(s)	125
MboI	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
MflI	RGATCY	2 cut(s)	357, 1105
MhlI	GDGCHC	1 cut(s)	143
MlyI	GAGTC	4 cut(s)	303, 550, 566, 1817
MmeI	TCCRAC	2 cut(s)	111, 1866
Mph1103I	ATGCAT	3 cut(s)	529, 1014, 1592
MseI	TTAA	4 cut(s)	956, 1131, 1214, 1413
MslI	CAYNNNNRTG	1 cut(s)	1310
MspA1I	CMGCKG	1 cut(s)	1349
MspI	CCGG	3 cut(s)	27, 538, 1037
Mva1269I	GAATGC	2 cut(s)	30, 619
MwoI	GCNNNNNNNGC	4 cut(s)	116, 147, 1522, 1850
NdeII	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
NlaIII	CATG	9 cut(s)	675, 746, 763, 862, 1016, 1097, 1315, 1529, 1754
NlaIV	GGNNCC	5 cut(s)	140, 141, 157, 187, 468
NmuCI	GTSAC	2 cut(s)	76, 710
NsiI	ATGCAT	3 cut(s)	529, 1014, 1592
PctI	GAATGC	2 cut(s)	30, 619
PfeI	GAWTC	9 cut(s)	9, 323, 962, 1027, 1067, 1167, 1399, 1660, 1873
PinAI	ACCGGT	1 cut(s)	1036
Ple19I	CGATCG	1 cut(s)	1147
PleI	GAGTC	4 cut(s)	302, 549, 566, 1816
PpsI	GAGTC	4 cut(s)	302, 549, 566, 1816
PsiI	TTATAA	1 cut(s)	1292
Psp1406I	AACGTT	1 cut(s)	336
PspEI	GGTNACC	1 cut(s)	1675
PspFI	CCCAGC	1 cut(s)	418
PspN4I	GGNNCC	5 cut(s)	140, 141, 157, 187, 468
PspOMI	GGGCCC	1 cut(s)	139
PspPI	GGNCC	4 cut(s)	139, 140, 467, 1315
PstNI	CAGNNNCTG	2 cut(s)	225, 941
PsuI	RGATCY	2 cut(s)	357, 1105
PvuI	CGATCG	1 cut(s)	1147
PvuII	CAGCTG	1 cut(s)	1349
RsaI	GTAC	2 cut(s)	1035, 1792
RsaNI	GTAC	2 cut(s)	1034, 1791
RseI	CAYNNNNRTG	1 cut(s)	1310
SaqAI	TTAA	4 cut(s)	956, 1131, 1214, 1413
Sau3AI	GATC	9 cut(s)	18, 357, 459, 977, 1105, 1123, 1144, 1236, 1687
Sau96I	GGNCC	4 cut(s)	139, 140, 467, 1315
SchI	GAGTC	4 cut(s)	303, 550, 566, 1817
SduI	GDGCHC	1 cut(s)	143
SfaNI	GCATC	6 cut(s)	514, 688, 1231, 1577, 1862, 1902
SfcI	CTRYAG	1 cut(s)	105
SinI	GGWCC	1 cut(s)	1315
SmiMI	CAYNNNNRTG	1 cut(s)	1310
SsiI	CCGC	6 cut(s)	6, 123, 236, 973, 1733, 1859
SspMI	CTAG	6 cut(s)	95, 381, 456, 1103, 1431, 1921
StyI	CCWWGG	2 cut(s)	470, 1176
TaaI	ACNGT	6 cut(s)	106, 565, 686, 841, 1785, 1838
TaiI	ACGT	2 cut(s)	339, 832
TaqI	TCGA	6 cut(s)	39, 60, 247, 798, 1143, 1239
TauI	GCSGC	3 cut(s)	125, 239, 976
TfiI	GAWTC	9 cut(s)	9, 323, 962, 1027, 1067, 1167, 1399, 1660, 1873
Tru1I	TTAA	4 cut(s)	956, 1131, 1214, 1413
Tru9I	TTAA	4 cut(s)	956, 1131, 1214, 1413
TscAI	CASTG	6 cut(s)	109, 570, 691, 933, 990, 1606
TseFI	GTSAC	2 cut(s)	76, 710
TseI	GCWGC	8 cut(s)	119, 283, 667, 1082, 1346, 1516, 1757, 1841
Tsp45I	GTSAC	2 cut(s)	76, 710
TspDTI	ATGAA	6 cut(s)	542, 759, 875, 1550, 1647, 1767
TspGWI	ACGGA	1 cut(s)	148
TspRI	CASTG	6 cut(s)	109, 570, 691, 933, 990, 1606
VpaK11BI	GGWCC	1 cut(s)	1315
XapI	RAATTY	3 cut(s)	993, 1255, 1822
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1309
XspI	CTAG	6 cut(s)	95, 381, 456, 1103, 1431, 1921
Zsp2I	ATGCAT	3 cut(s)	529, 1014, 1592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.