Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

4324256 .. 4328203

Gene Structure 3948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1061700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGTGCGGAATCGCTTTGATCATTTGCGGCATTCGTTTTCATCTATCGTCCCTATTTCTCAATTCCACACTTCCTGTCCTCAACTCCGACCAACTTGCATTCGACATTGACGACCTCAGAGAAGCTTTACGGAGGAGGGGCCCCGATAGCTTAGGCAGCACGAAGCTTCTTCTCCATTCACCCATTTTGAATCGTCTCCCGGTGAAAGAAATTGTGTCTTCCATCGAGGGGGAAGTAAAGGAGGAGTCTAGTTGCGAAGGCGATGAAGGCAATTGTTTCTCTTTGGAAAATGGTCGAACACCTCATTTGCTTACCCATTTCAGAACCGTTCCCTGTTCGTCTGCTGAGTTGCACTTCCTGGGTGCCACTTTGCAGCTCAGGGGAGTAAGCCCTGTAGTTCAGCCATTGATAGATTCGGCCAAGAATATCCTTGTTTATAATGGTGAAATCTTTGGTGGAATAGATATTGGTAGTGATGAGAACGATGGCGAAGTCCTTCTGCAGTTGCTGGGAGAGTGCTGTTCGGGTTCGATTCCAGGTGTTCTTTCTAGGATCAAAGGGCCTTGGGCTATCATCTATTGGCAGGAAAGTGCAAAAACTCTGTGGTTTGCTCGAGATGCGTTTGGTAGGCGGAGTCTTCTTGTTCACTGGCCAACGGAGGAGGACTCTCGGCTTCTGCTATCATCTGTTTCACCTGTTTCTTCAAATGAACATTCTTCTGTAGATATTGAAGCTGAAAATGGAACTACGAAGCTGAATTTTTGGGAAGAGCTCCGTTGTGGAATATACAGTATATCTATGGATGCTCCAGATGTTGATGGGGTTTTGGTTGGTGAAGTGAAAAAGCATGATTGGACTAATCCTGTGCTAGAAGAACTGATTAAATGGGAGAGAACTTATGTTGAACCTAAACCTGAAGATTTACATATTTCCCATTCGAAGAGTCTTACAGGGAAACATGATACGCGCTTGGTTAACTCAGATATAGTACCAATTAAATCAGGGTCTATACAAGTTTCAATTTCAGTGCCAGCACAGGCGGTGCTGATTGCTTTAAGGGAATCTGTTATGCGTCGCTCTTCACTGCACACAGTTTTTCAGGCAGGTAGGCTTGATATGAGGAAGGAAATTGTACCGGTGGCAGTTCTTTTTTCCGGTGGATTGGATTCTATGATAATTGCAGCATTATTGCATCAGTGCCTTGATCCAAGCTATGATATTGATCTACTTAATGTGAGCTTTGATGGTCGGTCTGCTCCTGACAGAATCTCTGCCAGGGCAGGAGTAAACGAACTGAGAAGAATTGCACCTTCAAGAAAGTGGAAGCTTGTGGAGATCAATGCTGAATTGTCAACATTGATCTTTGAAACAAAGCATGTCATGTCGCTTATAAATCCTGCCAACACCTACATGGACCTGAATATAGGAATAGCTTTGTGGCTTGCTGCTGGTGGCGATGGATGGGTATATGAGGAAAATACCAATTATAATGATGAGGATTGTCGATGCATTAAGTACAAGTCCAAGGCTAGGATTCTTCTTGTTGGTTCGGGTGCAGATGAGCAATGCGCTGGCTATGGTAGGCACAGAACGAAATATAGAAATGGCAGCTGGCTTGCACTGAACGAGGAAATGAAATTGGACATGCAGAGAATTTGGAAAAGAAACCTTGGGAGAGATGATAGGTGCATTGCTGACCATGGCAAGGAGGCAAGATTTCCGTTCCTGGATGAAGATGTTATAAGAATTTTGCTTGGGTTTCCTCTCTGGGAGGTTACCAACCTTGATCAGCCGAGTGGGATAGGGGATAAGAAGATTCTGAGAGAGGTTGCAGAATTGCTTGGTTTGCATGAAGCTGCTTCTCTGCCTAAAAGAGCTATACAGTTTGGTTCAAGAATTGCAAGGGAATCAAACCGAAAGAATTATGGAAGTAACCGTGCAGCAAATCAAGCATCTGCAGGATCAGTACAGGTGATTGAATTATGGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

664

Amino Acids

73.75

Weight (kDa)

5.8

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	131 - 218	1e-05	Glutamine amidotransferase domain
GATase_7	PF13537	134 - 229	8.8e-09	Glutamine amidotransferase domain
Asn_synthase	PF00733	536 - 614	7.8e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	438, 1391, 1488, 1742
Acc36I	ACCTGC	1 cut(s)	1094
AccB1I	GGYRCC	1 cut(s)	362
AccII	CGCG	1 cut(s)	967
AciI	CCGC	4 cut(s)	6, 27, 631, 1040
AclWI	GGATC	3 cut(s)	560, 1199, 1969
AcoI	YGGCCR	2 cut(s)	417, 650
AcsI	RAATTY	3 cut(s)	757, 1653, 1746
AcuI	CTGAAG	1 cut(s)	936
AfaI	GTAC	4 cut(s)	990, 1134, 1517, 1968
AfiI	CCNNNNNNNGG	3 cut(s)	228, 1530, 1705
AgeI	ACCGGT	1 cut(s)	1135
AgsI	TTSAA	9 cut(s)	190, 705, 731, 905, 1020, 1314, 1367, 1893, 1979
AjnI	CCWGG	4 cut(s)	357, 535, 1274, 1725
Alw21I	GWGCWC	1 cut(s)	774
Alw26I	GTCTC	1 cut(s)	200
AlwI	GGATC	3 cut(s)	560, 1199, 1969
AlwNI	CAGNNNCTG	1 cut(s)	508
Ama87I	CYCGRG	1 cut(s)	612
AoxI	GGCC	4 cut(s)	139, 417, 560, 650
ApaI	GGGCCC	1 cut(s)	143
ApeKI	GCWGC	7 cut(s)	156, 373, 1181, 1445, 1608, 1856, 1940
ApoI	RAATTY	3 cut(s)	757, 1653, 1746
AsiGI	ACCGGT	1 cut(s)	1135
Asp700I	GAANNNNTTC	1 cut(s)	495
AspLEI	GCGC	2 cut(s)	969, 1571
AspS9I	GGNCC	4 cut(s)	139, 140, 560, 1414
AsuC2I	CCSGG	1 cut(s)	200
AsuHPI	GGTGA	6 cut(s)	171, 214, 455, 684, 845, 1984
AsuII	TTCGAA	1 cut(s)	938
AvaI	CYCGRG	1 cut(s)	612
AvaII	GGWCC	1 cut(s)	1414
BaeGI	GKGCMC	1 cut(s)	143
BalI	TGGCCA	1 cut(s)	652
BanI	GGYRCC	1 cut(s)	362
BanII	GRGCYC	2 cut(s)	143, 774
BbsI	GAAGAC	2 cut(s)	210, 629
Bbv12I	GWGCWC	1 cut(s)	774
BbvI	GCAGC	7 cut(s)	168, 385, 1193, 1432, 1620, 1843, 1952
BccI	CCATC	6 cut(s)	230, 479, 812, 1238, 1451, 1455
BcgI	CGANNNNNNTGC	2 cut(s)	77, 111
BciT130I	CCWGG	4 cut(s)	359, 537, 1276, 1727
BclI	TGATCA	2 cut(s)	18, 1786
BcnI	CCSGG	1 cut(s)	200
BcoDI	GTCTC	1 cut(s)	200
BfaI	CTAG	4 cut(s)	249, 549, 869, 1530
BfmI	CTRYAG	4 cut(s)	393, 500, 720, 1956
BfuAI	ACCTGC	1 cut(s)	1094
BisI	GCNGC	8 cut(s)	28, 157, 374, 1182, 1446, 1609, 1857, 1941
BlsI	GCNGC	8 cut(s)	29, 158, 375, 1183, 1447, 1610, 1858, 1942
Bme1390I	CCNGG	5 cut(s)	200, 359, 537, 1276, 1727
Bme18I	GGWCC	1 cut(s)	1414
BmeT110I	CYCGRG	1 cut(s)	612
BmgT120I	GGNCC	4 cut(s)	139, 140, 560, 1414
BmiI	GGNNCC	4 cut(s)	140, 141, 142, 364
BmrFI	CCNGG	5 cut(s)	200, 359, 537, 1276, 1727
BmsI	GCATC	5 cut(s)	607, 793, 1201, 1496, 1961
BpiI	GAAGAC	2 cut(s)	210, 629
BplI	GAGNNNNNCTC	2 cut(s)	650, 682
BpmI	CTGGAG	1 cut(s)	792
Bpu10I	CCTNAGC	2 cut(s)	151, 377
Bpu14I	TTCGAA	1 cut(s)	938
BpuMI	CCSGG	1 cut(s)	200
BsaBI	GATNNNNATC	1 cut(s)	1221
BsaJI	CCNNGG	6 cut(s)	358, 563, 1275, 1524, 1669, 1699
BsaWI	WCCGGW	2 cut(s)	1135, 1154
Bsc4I	CCNNNNNNNGG	3 cut(s)	228, 1530, 1705
Bse118I	RCCGGY	1 cut(s)	1135
Bse1I	ACTGG	1 cut(s)	653
Bse3DI	GCAATG	2 cut(s)	1571, 1689
Bse8I	GATNNNNATC	1 cut(s)	1221
BseBI	CCWGG	4 cut(s)	359, 537, 1276, 1727
BseDI	CCNNGG	6 cut(s)	358, 563, 1275, 1524, 1669, 1699
BseGI	GGATG	3 cut(s)	808, 1466, 1735
BseJI	GATNNNNATC	1 cut(s)	1221
BseLI	CCNNNNNNNGG	3 cut(s)	228, 1530, 1705
BseMI	GCAATG	2 cut(s)	1571, 1689
BseMII	CTCAG	6 cut(s)	130, 336, 391, 993, 1286, 1811
BseNI	ACTGG	1 cut(s)	653
BseRI	GAGGAG	3 cut(s)	148, 257, 674
BseSI	GKGCMC	1 cut(s)	143
BseXI	GCAGC	7 cut(s)	168, 385, 1193, 1432, 1620, 1843, 1952
BseYI	CCCAGC	1 cut(s)	508
BsgI	GTGCAG	3 cut(s)	1070, 1575, 1959
Bsh1236I	CGCG	1 cut(s)	967
BshFI	GGCC	4 cut(s)	141, 419, 562, 652
BshNI	GGYRCC	1 cut(s)	362
BshTI	ACCGGT	1 cut(s)	1135
BsiHKAI	GWGCWC	1 cut(s)	774
BsiHKCI	CYCGRG	1 cut(s)	612
BsiSI	CCGG	3 cut(s)	200, 1136, 1155
BslFI	GGGAC	1 cut(s)	34
BslI	CCNNNNNNNGG	3 cut(s)	228, 1530, 1705
BsmAI	GTCTC	1 cut(s)	200
BsmBI	CGTCTC	1 cut(s)	200
BsmFI	GGGAC	1 cut(s)	34
BsmI	GAATGC	2 cut(s)	30, 98
BsnI	GGCC	4 cut(s)	141, 419, 562, 652
BsoBI	CYCGRG	1 cut(s)	612
Bsp119I	TTCGAA	1 cut(s)	938
Bsp120I	GGGCCC	1 cut(s)	139
Bsp1286I	GDGCHC	2 cut(s)	143, 774
Bsp143I	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
Bsp19I	CCATGG	1 cut(s)	1699
BspACI	CCGC	4 cut(s)	6, 27, 631, 1040
BspANI	GGCC	4 cut(s)	141, 419, 562, 652
BspCNI	CTCAG	6 cut(s)	129, 337, 390, 992, 1287, 1812
BspFNI	CGCG	1 cut(s)	967
BspLI	GGNNCC	4 cut(s)	140, 141, 142, 364
BspMAI	CTGCAG	2 cut(s)	504, 1960
BspMI	ACCTGC	1 cut(s)	1094
BspPI	GGATC	3 cut(s)	560, 1199, 1969
BspQI	GCTCTTC	2 cut(s)	762, 1084
BspT104I	TTCGAA	1 cut(s)	938
BspT107I	GGYRCC	1 cut(s)	362
BsrDI	GCAATG	2 cut(s)	1571, 1689
BsrFI	RCCGGY	1 cut(s)	1135
BsrI	ACTGG	1 cut(s)	653
BssAI	RCCGGY	1 cut(s)	1135
BssECI	CCNNGG	6 cut(s)	358, 563, 1275, 1524, 1669, 1699
BssMI	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
BssT1I	CCWWGG	4 cut(s)	563, 1524, 1669, 1699
Bst2UI	CCWGG	4 cut(s)	359, 537, 1276, 1727
Bst4CI	ACNGT	5 cut(s)	328, 791, 1093, 1884, 1937
Bst6I	CTCTTC	3 cut(s)	762, 935, 1084
BstBI	TTCGAA	1 cut(s)	938
BstC8I	GCNNGC	5 cut(s)	1032, 1443, 1573, 1613, 1617
BstDEI	CTNAG	7 cut(s)	116, 151, 345, 377, 979, 1295, 1820
BstDSI	CCRYGG	1 cut(s)	1699
BstEII	GGTNACC	1 cut(s)	1774
BstF5I	GGATG	3 cut(s)	808, 1466, 1735
BstFNI	CGCG	1 cut(s)	967
BstHHI	GCGC	2 cut(s)	969, 1571
BstKTI	GATC	8 cut(s)	21, 555, 1207, 1225, 1338, 1362, 1789, 1964
BstMAI	GTCTC	1 cut(s)	200
BstMBI	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
BstMWI	GCNNNNNNNGC	6 cut(s)	147, 156, 267, 617, 1846, 1949
BstNI	CCWGG	4 cut(s)	359, 537, 1276, 1727
BstNSI	RCATGY	2 cut(s)	1379, 1648
BstPI	GGTNACC	1 cut(s)	1774
BstSCI	CCNGG	5 cut(s)	198, 357, 535, 1274, 1725
BstSFI	CTRYAG	4 cut(s)	393, 500, 720, 1956
BstSLI	GKGCMC	1 cut(s)	143
BstUI	CGCG	1 cut(s)	967
BstV1I	GCAGC	7 cut(s)	168, 385, 1193, 1432, 1620, 1843, 1952
BstV2I	GAAGAC	2 cut(s)	210, 629
BsuRI	GGCC	4 cut(s)	141, 419, 562, 652
BtgI	CCRYGG	1 cut(s)	1699
BtgZI	GCGATG	2 cut(s)	276, 1470
BtsCI	GGATG	3 cut(s)	808, 1466, 1735
BtsI	GCAGTG	1 cut(s)	1082
BtsIMutI	CAGTG	5 cut(s)	646, 1032, 1082, 1202, 1619
BveI	ACCTGC	1 cut(s)	1094
Cac8I	GCNNGC	5 cut(s)	1032, 1443, 1573, 1613, 1617
CaiI	CAGNNNCTG	1 cut(s)	508
CfoI	GCGC	2 cut(s)	969, 1571
Cfr10I	RCCGGY	1 cut(s)	1135
Cfr13I	GGNCC	4 cut(s)	139, 140, 560, 1414
CseI	GACGC	1 cut(s)	1061
Csp6I	GTAC	4 cut(s)	989, 1133, 1516, 1967
CspAI	ACCGGT	1 cut(s)	1135
CviAII	CATG	8 cut(s)	848, 959, 1376, 1381, 1411, 1645, 1700, 1850
CviQI	GTAC	4 cut(s)	989, 1133, 1516, 1967
DdeI	CTNAG	7 cut(s)	116, 151, 345, 377, 979, 1295, 1820
DpnI	GATC	8 cut(s)	20, 554, 1206, 1224, 1337, 1361, 1788, 1963
DpnII	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
EaeI	YGGCCR	2 cut(s)	417, 650
Eam1104I	CTCTTC	3 cut(s)	762, 935, 1084
EarI	CTCTTC	3 cut(s)	762, 935, 1084
EciI	GGCGGA	1 cut(s)	646
Ecl136II	GAGCTC	1 cut(s)	772
Eco130I	CCWWGG	4 cut(s)	563, 1524, 1669, 1699
Eco24I	GRGCYC	2 cut(s)	143, 774
Eco47I	GGWCC	1 cut(s)	1414
Eco53kI	GAGCTC	1 cut(s)	772
Eco57I	CTGAAG	1 cut(s)	936
Eco88I	CYCGRG	1 cut(s)	612
Eco91I	GGTNACC	1 cut(s)	1774
EcoICRI	GAGCTC	1 cut(s)	772
EcoO109I	RGGNCCY	3 cut(s)	139, 140, 560
EcoO65I	GGTNACC	1 cut(s)	1774
EcoRII	CCWGG	4 cut(s)	357, 535, 1274, 1725
EcoT14I	CCWWGG	4 cut(s)	563, 1524, 1669, 1699
EcoT22I	ATGCAT	1 cut(s)	1511
EcoT38I	GRGCYC	2 cut(s)	143, 774
ErhI	CCWWGG	4 cut(s)	563, 1524, 1669, 1699
Esp3I	CGTCTC	1 cut(s)	200
FaeI	CATG	8 cut(s)	851, 962, 1379, 1384, 1414, 1648, 1703, 1853
FaqI	GGGAC	1 cut(s)	34
FatI	CATG	8 cut(s)	847, 958, 1375, 1380, 1410, 1644, 1699, 1849
FbaI	TGATCA	2 cut(s)	18, 1786
Fnu4HI	GCNGC	8 cut(s)	28, 157, 374, 1182, 1446, 1609, 1857, 1941
FokI	GGATG	3 cut(s)	815, 1473, 1742
FriOI	GRGCYC	2 cut(s)	143, 774
Fsp4HI	GCNGC	8 cut(s)	28, 157, 374, 1182, 1446, 1609, 1857, 1941
FspBI	CTAG	4 cut(s)	249, 549, 869, 1530
GlaI	GCGC	2 cut(s)	968, 1570
GluI	GCNGC	8 cut(s)	28, 157, 374, 1182, 1446, 1609, 1857, 1941
GsaI	CCCAGC	1 cut(s)	512
GsuI	CTGGAG	1 cut(s)	792
HaeIII	GGCC	4 cut(s)	141, 419, 562, 652
HapII	CCGG	3 cut(s)	200, 1136, 1155
HgaI	GACGC	1 cut(s)	1061
HhaI	GCGC	2 cut(s)	969, 1571
Hin1II	CATG	8 cut(s)	851, 962, 1379, 1384, 1414, 1648, 1703, 1853
Hin6I	GCGC	2 cut(s)	967, 1569
HinP1I	GCGC	2 cut(s)	967, 1569
HincII	GTYRAC	2 cut(s)	976, 1353
HindII	GTYRAC	2 cut(s)	976, 1353
HindIII	AAGCTT	3 cut(s)	123, 164, 1325
HpaI	GTTAAC	1 cut(s)	976
HpaII	CCGG	3 cut(s)	200, 1136, 1155
HphI	GGTGA	6 cut(s)	171, 214, 455, 684, 845, 1984
Hpy166II	GTNNAC	4 cut(s)	646, 976, 1288, 1353
Hpy188I	TCNGA	5 cut(s)	88, 119, 323, 982, 1821
Hpy188III	TCNNGA	5 cut(s)	614, 809, 1259, 1314, 1893
Hpy8I	GTNNAC	4 cut(s)	646, 976, 1288, 1353
Hpy99I	CGWCG	1 cut(s)	1077
HpyAV	CCTTC	5 cut(s)	251, 260, 506, 1117, 1320
HpyCH4III	ACNGT	5 cut(s)	328, 791, 1093, 1884, 1937
HpyF10VI	GCNNNNNNNGC	6 cut(s)	147, 156, 267, 617, 1846, 1949
HpyF3I	CTNAG	7 cut(s)	116, 151, 345, 377, 979, 1295, 1820
Hsp92II	CATG	8 cut(s)	851, 962, 1379, 1384, 1414, 1648, 1703, 1853
HspAI	GCGC	2 cut(s)	967, 1569
Ksp22I	TGATCA	2 cut(s)	18, 1786
KspAI	GTTAAC	1 cut(s)	976
Kzo9I	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
LguI	GCTCTTC	2 cut(s)	762, 1084
LmnI	GCTCC	3 cut(s)	777, 811, 1261
Lsp1109I	GCAGC	7 cut(s)	168, 385, 1193, 1432, 1620, 1843, 1952
LweI	GCATC	5 cut(s)	607, 793, 1201, 1496, 1961
MaeI	CTAG	4 cut(s)	249, 549, 869, 1530
MaeIII	GTNAC	2 cut(s)	1774, 1931
MalI	GATC	8 cut(s)	20, 554, 1206, 1224, 1337, 1361, 1788, 1963
MboI	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
MfeI	CAATTG	1 cut(s)	271
MhlI	GDGCHC	2 cut(s)	143, 774
MlsI	TGGCCA	1 cut(s)	652
MluNI	TGGCCA	1 cut(s)	652
MlyI	GAGTC	4 cut(s)	254, 643, 659, 952
MmeI	TCCRAC	1 cut(s)	111
Mox20I	TGGCCA	1 cut(s)	652
Mph1103I	ATGCAT	1 cut(s)	1511
MroXI	GAANNNNTTC	1 cut(s)	495
MscI	TGGCCA	1 cut(s)	652
MseI	TTAA	6 cut(s)	882, 975, 996, 1055, 1230, 1512
MslI	CAYNNNNRTG	1 cut(s)	1409
Msp20I	TGGCCA	1 cut(s)	652
MspA1I	CMGCKG	1 cut(s)	1611
MspI	CCGG	3 cut(s)	200, 1136, 1155
MspR9I	CCNGG	5 cut(s)	200, 359, 537, 1276, 1727
MunI	CAATTG	1 cut(s)	271
Mva1269I	GAATGC	2 cut(s)	30, 98
MvaI	CCWGG	4 cut(s)	359, 537, 1276, 1727
MvnI	CGCG	1 cut(s)	967
MwoI	GCNNNNNNNGC	6 cut(s)	147, 156, 267, 617, 1846, 1949
NciI	CCSGG	1 cut(s)	200
NcoI	CCATGG	1 cut(s)	1699
NdeII	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
NlaIII	CATG	8 cut(s)	851, 962, 1379, 1384, 1414, 1648, 1703, 1853
NlaIV	GGNNCC	4 cut(s)	140, 141, 142, 364
NmeAIII	GCCGAG	2 cut(s)	649, 1818
NsiI	ATGCAT	1 cut(s)	1511
NspI	RCATGY	2 cut(s)	1379, 1648
NspV	TTCGAA	1 cut(s)	938
PaeR7I	CTCGAG	1 cut(s)	612
PciSI	GCTCTTC	2 cut(s)	762, 1084
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PctI	GAATGC	2 cut(s)	30, 98
PdmI	GAANNNNTTC	1 cut(s)	495
PfoI	TCCNGGA	1 cut(s)	1725
PinAI	ACCGGT	1 cut(s)	1135
PkrI	GCNGC	8 cut(s)	29, 158, 375, 1183, 1447, 1610, 1858, 1942
PleI	GAGTC	4 cut(s)	253, 642, 659, 951
PpsI	GAGTC	4 cut(s)	253, 642, 659, 951
PsiI	TTATAA	4 cut(s)	438, 1391, 1488, 1742
Psp124BI	GAGCTC	1 cut(s)	774
Psp6I	CCWGG	4 cut(s)	357, 535, 1274, 1725
PspEI	GGTNACC	1 cut(s)	1774
PspFI	CCCAGC	1 cut(s)	508
PspGI	CCWGG	4 cut(s)	357, 535, 1274, 1725
PspN4I	GGNNCC	4 cut(s)	140, 141, 142, 364
PspOMI	GGGCCC	1 cut(s)	139
PspPI	GGNCC	4 cut(s)	139, 140, 560, 1414
PstI	CTGCAG	2 cut(s)	504, 1960
PstNI	CAGNNNCTG	1 cut(s)	508
PvuII	CAGCTG	1 cut(s)	1611
RsaI	GTAC	4 cut(s)	990, 1134, 1517, 1968
RsaNI	GTAC	4 cut(s)	989, 1133, 1516, 1967
RseI	CAYNNNNRTG	1 cut(s)	1409
SacI	GAGCTC	1 cut(s)	774
SapI	GCTCTTC	2 cut(s)	762, 1084
SaqAI	TTAA	6 cut(s)	882, 975, 996, 1055, 1230, 1512
SatI	GCNGC	8 cut(s)	28, 157, 374, 1182, 1446, 1609, 1857, 1941
Sau3AI	GATC	8 cut(s)	18, 552, 1204, 1222, 1335, 1359, 1786, 1961
Sau96I	GGNCC	4 cut(s)	139, 140, 560, 1414
SchI	GAGTC	4 cut(s)	254, 643, 659, 952
ScrFI	CCNGG	5 cut(s)	200, 359, 537, 1276, 1727
SduI	GDGCHC	2 cut(s)	143, 774
SfaNI	GCATC	5 cut(s)	607, 793, 1201, 1496, 1961
SfcI	CTRYAG	4 cut(s)	393, 500, 720, 1956
Sfr274I	CTCGAG	1 cut(s)	612
SfuI	TTCGAA	1 cut(s)	938
SinI	GGWCC	1 cut(s)	1414
SlaI	CTCGAG	1 cut(s)	612
SmiMI	CAYNNNNRTG	1 cut(s)	1409
SmlI	CTYRAG	1 cut(s)	612
SmoI	CTYRAG	1 cut(s)	612
SsiI	CCGC	4 cut(s)	6, 27, 631, 1040
SspMI	CTAG	4 cut(s)	249, 549, 869, 1530
SstI	GAGCTC	1 cut(s)	774
StyD4I	CCNGG	5 cut(s)	198, 357, 535, 1274, 1725
StyI	CCWWGG	4 cut(s)	563, 1524, 1669, 1699
TaaI	ACNGT	5 cut(s)	328, 791, 1093, 1884, 1937
TaqI	TCGA	7 cut(s)	102, 225, 295, 530, 613, 938, 1504
TaqII	GACCGA	1 cut(s)	1239
TatI	WGTACW	2 cut(s)	1515, 1966
TauI	GCSGC	1 cut(s)	30
Tru1I	TTAA	6 cut(s)	882, 975, 996, 1055, 1230, 1512
Tru9I	TTAA	6 cut(s)	882, 975, 996, 1055, 1230, 1512
TscAI	CASTG	5 cut(s)	653, 1032, 1089, 1202, 1626
TseI	GCWGC	7 cut(s)	156, 373, 1181, 1445, 1608, 1856, 1940
TspDTI	ATGAA	6 cut(s)	29, 279, 723, 1649, 1746, 1866
TspGWI	ACGGA	4 cut(s)	145, 671, 764, 1710
TspRI	CASTG	5 cut(s)	653, 1032, 1089, 1202, 1626
VpaK11BI	GGWCC	1 cut(s)	1414
XapI	RAATTY	3 cut(s)	757, 1653, 1746
XceI	RCATGY	2 cut(s)	1379, 1648
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1408
XhoI	CTCGAG	1 cut(s)	612
XmnI	GAANNNNTTC	1 cut(s)	495
XspI	CTAG	4 cut(s)	249, 549, 869, 1530
Zsp2I	ATGCAT	1 cut(s)	1511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.