Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

58546581 .. 58551032

Gene Structure 4452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG364600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGTGCGGAATCGCTCTGATCATCTCCGGCATTCGCTTCGACTTATCGTCTCTGCTTCTGGACACCACACCTCCAGTCACCAACTCCGACCAACTAGAGTTCACTATAGCCGACCTCGCAGCCGCTCTGCGACGGAGGGGCCCAGATAGCTTAGGCACCAAGAAGCTTCTTCTTCACTCATCAGGCTCGGAAATCGTGTCTGGAGTAGAGGAGGAGTTCAACAACTGCTTCTCTGCGGCAAATGGTCGAACACCCTGTATGCATTTTTTTGGTGCTACTTTGCAGCTCAGGGGAGTCAACCCAGTAGTTCACCCATTGATAGATTCGTCTAAGAACGTTCTTGTCTATAATGGTGAGATCTTTGGTGGGATTGAGATTGCTAGTGAGGAGAATGACGGTGAAGTTCTAATGGGGTTGCTGGGAGGGTGTGAAGGTTGTGTGCCGAATGTTGTTTCTAGGATCAAGGGCCCTTGGGCTATCATCTATTGGCAGGAAAGTTCAAGAACATTATGGTTTGGGAGAGATGCATTTGGTCGCCGGAGTCTTCTTGTTCACTGGCAGACAGTGGAGGACTCTCGGTTTCTGTTATCATCAGTATCACCCGTTGCTTCAAATGAGCATTCTTCTGCTGAAAATGGAACAACCAAGCTTGGTTTTTGGGAGGAGCTGCCATGTGGAATATACAGTGTATCCATAGATGCTCCCAATGGTGACGGGAGTTTGGTTGGTGAAGTGAAAAAGCATGAATGGACTAATGCCATGCTAGAAGAACTGATAAAATGGGAGAGAACATCCGTCGAACCCAAACCCAAAGAGTTGCATATTTCACGTTGTGAGACTGTAAGAGGGCAACACGATATGAACTCACTTACTTCAGATATAGTGCCAATGCCATCTAAGTCAGGCAATATACAAGCTTCAATTTCAGTGCCAGCAGAGGCTGTGCTGATTGCTTTAAGGGAATCAGTTTTGCGGCGATCTTCACTGCACAGAATTTTTCAGGCAAGTATGCATGTGGAAAAGAATCTTGTACCGGTGGCAGTTCTTTTTTCTGGTGGATTGGATTCTATGATAATTGCAGCATTGTTGCATGACTGCCTAGATCCAAGCTATGAGATTGATCTACTTAATGTGAGCTTTGATGGTCAGTCTGCTCCAGATAGAATCTCTGCCAAGGCAGGAGTGAGCGAACTGAGAAGAATTGCACCCTTAAGAAAGTGGAAACTTGTGGAGATCAATGCTGATTCATCAAATTTGACCTTTGAAACAAAGCGTGTATTGTCTCTCATAAACCCTGCCAACACCTACATGGACCTAAATATAGGAATCGCGTTATGGCTGGCAGCTGGTGGGGATGGATGGGTTTATGAGGAAAATAGCAAATATAATGATGAGAATCGTCAAGGTGTTAGATACAAGTCAAAGGCTAGGATTGTCCTTGTTGGTTCAGGTGCAGATGAGCAATGTGCTGGCTATGGTAGGCACAGAACAAAATATAGAAGTGGAAGTTGGTTGCGACTGCATGAGGAAATGAAACTGGATATGCAAAGAATATGGAAAAGAAACCTTGGTAGAGATGATAGATGCATTGCTGACACTGGCAAGGAGGCAAGGTTTCCTTTTTTGGATGAAGATGTTATACGAACTTTATTGGGGATTCCTCTCTGGGAGGTTACCAACCTTGATCAACCAAGTGGAATAGGAGATAAGAAGATTTTGAGAGAGGTAATTGCTAATTCTTTTTGCAGCACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

584

Amino Acids

64.57

Weight (kDa)

5.73

Isoelectric Point (pI)

43.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	105 - 198	2e-08	Glutamine amidotransferase domain
Asn_synthase	PF00733	501 - 578	8.1e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	155
AccBSI	CCGCTC	1 cut(s)	125
AccII	CGCG	1 cut(s)	1331
AciI	CCGC	4 cut(s)	6, 123, 236, 973
AclI	AACGTT	1 cut(s)	336
AclWI	GGATC	2 cut(s)	467, 1097
AcsI	RAATTY	2 cut(s)	993, 1252
AcuI	CTGAAG	1 cut(s)	858
AdeI	CACNNNGTG	1 cut(s)	833
AfaI	GTAC	1 cut(s)	1032
AflII	CTTAAG	1 cut(s)	1210
AgeI	ACCGGT	1 cut(s)	1033
AgsI	TTSAA	5 cut(s)	220, 501, 612, 921, 1265
AhdI	GACNNNNNGTC	1 cut(s)	46
AjuI	GAANNNNNNNTTGG	2 cut(s)	1492, 1524
AluBI	AGCT	9 cut(s)	150, 166, 286, 649, 667, 917, 1110, 1137, 1346
AluI	AGCT	9 cut(s)	150, 166, 286, 649, 667, 917, 1110, 1137, 1346
Alw26I	GTCTC	3 cut(s)	54, 830, 1287
AlwI	GGATC	2 cut(s)	467, 1097
AlwNI	CAGNNNCTG	1 cut(s)	941
AoxI	GGCC	2 cut(s)	139, 466
ApaI	GGGCCC	2 cut(s)	143, 470
ApeKI	GCWGC	6 cut(s)	119, 283, 667, 1079, 1343, 1746
ApoI	RAATTY	2 cut(s)	993, 1252
AsiGI	ACCGGT	1 cut(s)	1033
AspS9I	GGNCC	5 cut(s)	139, 140, 466, 467, 1312
AsuHPI	GGTGA	7 cut(s)	70, 302, 365, 410, 591, 722, 740
AvaII	GGWCC	1 cut(s)	1312
BaeGI	GKGCMC	2 cut(s)	143, 470
BanI	GGYRCC	1 cut(s)	155
BanII	GRGCYC	2 cut(s)	143, 470
BarI	GAAGNNNNNNTAC	2 cut(s)	1623, 1655
BbsI	GAAGAC	1 cut(s)	536
BbvI	GCAGC	5 cut(s)	131, 295, 654, 1091, 1355
BccI	CCATC	4 cut(s)	901, 1136, 1349, 1353
BcgI	CGANNNNNNTGC	2 cut(s)	19, 53
BciVI	GTATCC	1 cut(s)	700
BclI	TGATCA	2 cut(s)	18, 1684
BcoDI	GTCTC	3 cut(s)	54, 830, 1287
BfaI	CTAG	6 cut(s)	95, 381, 456, 764, 1100, 1428
BfmI	CTRYAG	1 cut(s)	105
BfrI	CTTAAG	1 cut(s)	1210
BfuI	GTATCC	1 cut(s)	700
BglII	AGATCT	1 cut(s)	357
BisI	GCNGC	9 cut(s)	120, 123, 237, 284, 668, 974, 1080, 1344, 1747
BlsI	GCNGC	9 cut(s)	121, 124, 238, 285, 669, 975, 1081, 1345, 1748
Bme18I	GGWCC	1 cut(s)	1312
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgT120I	GGNCC	5 cut(s)	139, 140, 466, 467, 1312
BmiI	GGNNCC	4 cut(s)	140, 141, 157, 468
BmrI	ACTGGG	1 cut(s)	296
BmsI	GCATC	3 cut(s)	514, 688, 1574
BmuI	ACTGGG	1 cut(s)	296
BpiI	GAAGAC	1 cut(s)	536
BpmI	CTGGAG	3 cut(s)	57, 222, 1140
Bpu10I	CCTNAGC	2 cut(s)	151, 287
BsaBI	GATNNNNATC	1 cut(s)	1395
BsaJI	CCNNGG	3 cut(s)	470, 1173, 1567
BsaWI	WCCGGW	1 cut(s)	1033
BsaXI	ACNNNNNCTCC	4 cut(s)	55, 85, 1598, 1628
Bse118I	RCCGGY	1 cut(s)	1033
Bse1I	ACTGG	5 cut(s)	74, 302, 560, 1542, 1603
Bse3DI	GCAATG	2 cut(s)	1469, 1587
Bse8I	GATNNNNATC	1 cut(s)	1395
BseDI	CCNNGG	3 cut(s)	470, 1173, 1567
BseGI	GGATG	4 cut(s)	791, 1360, 1364, 1633
BseJI	GATNNNNATC	1 cut(s)	1395
BseMI	GCAATG	2 cut(s)	1469, 1587
BseMII	CTCAG	2 cut(s)	301, 1184
BseNI	ACTGG	5 cut(s)	74, 302, 560, 1542, 1603
BseRI	GAGGAG	4 cut(s)	224, 227, 401, 677
BseSI	GKGCMC	2 cut(s)	143, 470
BseXI	GCAGC	5 cut(s)	131, 295, 654, 1091, 1355
BseYI	CCCAGC	1 cut(s)	418
BsgI	GTGCAG	2 cut(s)	971, 1473
Bsh1236I	CGCG	1 cut(s)	1331
BshFI	GGCC	2 cut(s)	141, 468
BshNI	GGYRCC	1 cut(s)	155
BshTI	ACCGGT	1 cut(s)	1033
BsiSI	CCGG	3 cut(s)	27, 538, 1034
BsmAI	GTCTC	3 cut(s)	54, 830, 1287
BsmBI	CGTCTC	1 cut(s)	54
BsmI	GAATGC	2 cut(s)	30, 619
BsnI	GGCC	2 cut(s)	141, 468
Bsp120I	GGGCCC	2 cut(s)	139, 466
Bsp1286I	GDGCHC	2 cut(s)	143, 470
Bsp143I	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
BspACI	CCGC	4 cut(s)	6, 123, 236, 973
BspANI	GGCC	2 cut(s)	141, 468
BspCNI	CTCAG	2 cut(s)	300, 1185
BspFNI	CGCG	1 cut(s)	1331
BspLI	GGNNCC	4 cut(s)	140, 141, 157, 468
BspPI	GGATC	2 cut(s)	467, 1097
BspT107I	GGYRCC	1 cut(s)	155
BspTI	CTTAAG	1 cut(s)	1210
BsrBI	CCGCTC	1 cut(s)	125
BsrDI	GCAATG	2 cut(s)	1469, 1587
BsrFI	RCCGGY	1 cut(s)	1033
BsrI	ACTGG	5 cut(s)	74, 302, 560, 1542, 1603
BssAI	RCCGGY	1 cut(s)	1033
BssECI	CCNNGG	3 cut(s)	470, 1173, 1567
BssMI	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
BssT1I	CCWWGG	3 cut(s)	470, 1173, 1567
Bst4CI	ACNGT	4 cut(s)	398, 565, 686, 841
BstAFI	CTTAAG	1 cut(s)	1210
BstC8I	GCNNGC	3 cut(s)	933, 1341, 1471
BstDEI	CTNAG	5 cut(s)	151, 287, 330, 897, 1193
BstEII	GGTNACC	1 cut(s)	1672
BstF5I	GGATG	4 cut(s)	791, 1360, 1364, 1633
BstFNI	CGCG	1 cut(s)	1331
BstKTI	GATC	8 cut(s)	21, 360, 462, 980, 1105, 1123, 1236, 1687
BstMAI	GTCTC	3 cut(s)	54, 830, 1287
BstMBI	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
BstMWI	GCNNNNNNNGC	2 cut(s)	116, 147
BstNSI	RCATGY	1 cut(s)	1016
BstPI	GGTNACC	1 cut(s)	1672
BstSFI	CTRYAG	1 cut(s)	105
BstSLI	GKGCMC	2 cut(s)	143, 470
BstUI	CGCG	1 cut(s)	1331
BstV1I	GCAGC	5 cut(s)	131, 295, 654, 1091, 1355
BstV2I	GAAGAC	1 cut(s)	536
BstX2I	RGATCY	2 cut(s)	357, 1102
BstYI	RGATCY	2 cut(s)	357, 1102
BsuI	GTATCC	1 cut(s)	700
BsuRI	GGCC	2 cut(s)	141, 468
BtsCI	GGATG	4 cut(s)	791, 1360, 1364, 1633
BtsI	GCAGTG	1 cut(s)	983
BtsIMutI	CAGTG	6 cut(s)	553, 570, 691, 933, 983, 1596
Cac8I	GCNNGC	3 cut(s)	933, 1341, 1471
CaiI	CAGNNNCTG	1 cut(s)	941
Cfr10I	RCCGGY	1 cut(s)	1033
Cfr13I	GGNCC	5 cut(s)	139, 140, 466, 467, 1312
Csp6I	GTAC	1 cut(s)	1031
CspAI	ACCGGT	1 cut(s)	1033
CviAII	CATG	7 cut(s)	672, 743, 760, 1013, 1091, 1309, 1523
CviQI	GTAC	1 cut(s)	1031
DdeI	CTNAG	5 cut(s)	151, 287, 330, 897, 1193
DpnI	GATC	8 cut(s)	20, 359, 461, 979, 1104, 1122, 1235, 1686
DpnII	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
DraIII	CACNNNGTG	1 cut(s)	833
DriI	GACNNNNNGTC	1 cut(s)	46
Eam1105I	GACNNNNNGTC	1 cut(s)	46
Eco130I	CCWWGG	3 cut(s)	470, 1173, 1567
Eco24I	GRGCYC	2 cut(s)	143, 470
Eco47I	GGWCC	1 cut(s)	1312
Eco57I	CTGAAG	1 cut(s)	858
Eco91I	GGTNACC	1 cut(s)	1672
EcoO109I	RGGNCCY	3 cut(s)	139, 466, 467
EcoO65I	GGTNACC	1 cut(s)	1672
EcoT14I	CCWWGG	3 cut(s)	470, 1173, 1567
EcoT22I	ATGCAT	4 cut(s)	264, 529, 1014, 1589
EcoT38I	GRGCYC	2 cut(s)	143, 470
ErhI	CCWWGG	3 cut(s)	470, 1173, 1567
Esp3I	CGTCTC	1 cut(s)	54
FaeI	CATG	7 cut(s)	675, 746, 763, 1016, 1094, 1312, 1526
FalI	AAGNNNNNCTT	2 cut(s)	1603, 1635
FatI	CATG	7 cut(s)	671, 742, 759, 1012, 1090, 1308, 1522
FbaI	TGATCA	2 cut(s)	18, 1684
Fnu4HI	GCNGC	9 cut(s)	120, 123, 237, 284, 668, 974, 1080, 1344, 1747
FokI	GGATG	4 cut(s)	778, 1367, 1371, 1640
FriOI	GRGCYC	2 cut(s)	143, 470
Fsp4HI	GCNGC	9 cut(s)	120, 123, 237, 284, 668, 974, 1080, 1344, 1747
FspBI	CTAG	6 cut(s)	95, 381, 456, 764, 1100, 1428
GluI	GCNGC	9 cut(s)	120, 123, 237, 284, 668, 974, 1080, 1344, 1747
GsaI	CCCAGC	1 cut(s)	422
GsuI	CTGGAG	3 cut(s)	57, 222, 1140
HaeIII	GGCC	2 cut(s)	141, 468
HapII	CCGG	3 cut(s)	27, 538, 1034
Hin1II	CATG	7 cut(s)	675, 746, 763, 1016, 1094, 1312, 1526
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HindIII	AAGCTT	3 cut(s)	164, 647, 915
HpaII	CCGG	3 cut(s)	27, 538, 1034
HphI	GGTGA	7 cut(s)	70, 302, 365, 410, 591, 722, 740
Hpy166II	GTNNAC	4 cut(s)	102, 298, 310, 553
Hpy188I	TCNGA	4 cut(s)	18, 88, 190, 877
Hpy188III	TCNNGA	4 cut(s)	59, 201, 501, 1157
Hpy8I	GTNNAC	4 cut(s)	102, 298, 310, 553
Hpy99I	CGWCG	2 cut(s)	135, 800
HpyAV	CCTTC	1 cut(s)	425
HpyCH4III	ACNGT	4 cut(s)	398, 565, 686, 841
HpyCH4IV	ACGT	2 cut(s)	336, 829
HpyF10VI	GCNNNNNNNGC	2 cut(s)	116, 147
HpyF3I	CTNAG	5 cut(s)	151, 287, 330, 897, 1193
HpySE526I	ACGT	2 cut(s)	336, 829
Hsp92II	CATG	7 cut(s)	675, 746, 763, 1016, 1094, 1312, 1526
Ksp22I	TGATCA	2 cut(s)	18, 1684
Kzo9I	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
LmnI	GCTCC	3 cut(s)	664, 706, 1159
Lsp1109I	GCAGC	5 cut(s)	131, 295, 654, 1091, 1355
LweI	GCATC	3 cut(s)	514, 688, 1574
MaeI	CTAG	6 cut(s)	95, 381, 456, 764, 1100, 1428
MaeII	ACGT	2 cut(s)	336, 829
MaeIII	GTNAC	3 cut(s)	76, 710, 1672
MalI	GATC	8 cut(s)	20, 359, 461, 979, 1104, 1122, 1235, 1686
MbiI	CCGCTC	1 cut(s)	125
MboI	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
MboII	GAAGA	9 cut(s)	161, 164, 536, 615, 779, 972, 1209, 1643, 1723
MflI	RGATCY	2 cut(s)	357, 1102
MhlI	GDGCHC	2 cut(s)	143, 470
MluCI	AATT	7 cut(s)	921, 993, 1074, 1200, 1252, 1728, 1735
MlyI	GAGTC	3 cut(s)	303, 550, 566
MmeI	TCCRAC	1 cut(s)	111
Mph1103I	ATGCAT	4 cut(s)	264, 529, 1014, 1589
MseI	TTAA	3 cut(s)	956, 1128, 1211
MslI	CAYNNNNRTG	1 cut(s)	1307
MspA1I	CMGCKG	1 cut(s)	1346
MspCI	CTTAAG	1 cut(s)	1210
MspI	CCGG	3 cut(s)	27, 538, 1034
Mva1269I	GAATGC	2 cut(s)	30, 619
MvnI	CGCG	1 cut(s)	1331
MwoI	GCNNNNNNNGC	2 cut(s)	116, 147
NdeII	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
NlaIII	CATG	7 cut(s)	675, 746, 763, 1016, 1094, 1312, 1526
NlaIV	GGNNCC	4 cut(s)	140, 141, 157, 468
NmuCI	GTSAC	2 cut(s)	76, 710
NsiI	ATGCAT	4 cut(s)	264, 529, 1014, 1589
NspI	RCATGY	1 cut(s)	1016
PctI	GAATGC	2 cut(s)	30, 619
PinAI	ACCGGT	1 cut(s)	1033
PkrI	GCNGC	9 cut(s)	121, 124, 238, 285, 669, 975, 1081, 1345, 1748
PleI	GAGTC	3 cut(s)	302, 549, 566
PpsI	GAGTC	3 cut(s)	302, 549, 566
Psp1406I	AACGTT	1 cut(s)	336
PspEI	GGTNACC	1 cut(s)	1672
PspFI	CCCAGC	1 cut(s)	418
PspN4I	GGNNCC	4 cut(s)	140, 141, 157, 468
PspOMI	GGGCCC	2 cut(s)	139, 466
PspPI	GGNCC	5 cut(s)	139, 140, 466, 467, 1312
PstNI	CAGNNNCTG	1 cut(s)	941
PsuI	RGATCY	2 cut(s)	357, 1102
PvuII	CAGCTG	1 cut(s)	1346
RsaI	GTAC	1 cut(s)	1032
RsaNI	GTAC	1 cut(s)	1031
RseI	CAYNNNNRTG	1 cut(s)	1307
SaqAI	TTAA	3 cut(s)	956, 1128, 1211
SatI	GCNGC	9 cut(s)	120, 123, 237, 284, 668, 974, 1080, 1344, 1747
Sau3AI	GATC	8 cut(s)	18, 357, 459, 977, 1102, 1120, 1233, 1684
Sau96I	GGNCC	5 cut(s)	139, 140, 466, 467, 1312
SchI	GAGTC	3 cut(s)	303, 550, 566
SduI	GDGCHC	2 cut(s)	143, 470
SfaNI	GCATC	3 cut(s)	514, 688, 1574
SfcI	CTRYAG	1 cut(s)	105
SinI	GGWCC	1 cut(s)	1312
SmiMI	CAYNNNNRTG	1 cut(s)	1307
SmlI	CTYRAG	1 cut(s)	1210
SmoI	CTYRAG	1 cut(s)	1210
Sse9I	AATT	7 cut(s)	921, 993, 1074, 1200, 1252, 1728, 1735
SsiI	CCGC	4 cut(s)	6, 123, 236, 973
SspMI	CTAG	6 cut(s)	95, 381, 456, 764, 1100, 1428
StyI	CCWWGG	3 cut(s)	470, 1173, 1567
TaaI	ACNGT	4 cut(s)	398, 565, 686, 841
TaiI	ACGT	2 cut(s)	339, 832
TaqI	TCGA	3 cut(s)	39, 247, 798
TasI	AATT	7 cut(s)	921, 993, 1074, 1200, 1252, 1728, 1735
TauI	GCSGC	3 cut(s)	125, 239, 976
Tru1I	TTAA	3 cut(s)	956, 1128, 1211
Tru9I	TTAA	3 cut(s)	956, 1128, 1211
TscAI	CASTG	6 cut(s)	560, 570, 691, 933, 990, 1603
TseFI	GTSAC	2 cut(s)	76, 710
TseI	GCWGC	6 cut(s)	119, 283, 667, 1079, 1343, 1746
Tsp45I	GTSAC	2 cut(s)	76, 710
TspDTI	ATGAA	5 cut(s)	759, 875, 1236, 1547, 1644
TspGWI	ACGGA	2 cut(s)	148, 784
TspRI	CASTG	6 cut(s)	560, 570, 691, 933, 990, 1603
Vha464I	CTTAAG	1 cut(s)	1210
VpaK11BI	GGWCC	1 cut(s)	1312
XapI	RAATTY	2 cut(s)	993, 1252
XceI	RCATGY	1 cut(s)	1016
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1306
XspI	CTAG	6 cut(s)	95, 381, 456, 764, 1100, 1428
Zsp2I	ATGCAT	4 cut(s)	264, 529, 1014, 1589

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.