Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00751

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

186864 .. 190862

Gene Structure 3999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G016270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGTGCGGAATCGCTCTGATCATCTCCGGCATTCGCTTCGACTTATCGTCTCTGCTTCTCGACACCACACCTCCAGTCACCAACTCCGACCAACTAGAGTTCACTATAGCCGACCTCGCAGCCGCTCTGCGACGGAGGGGCCCAGATAGCTTAGGCACCAAGAAGCTTCTTCTTCACTCATCAGGCTCCGAAATCGTGTCTGGAGTAGAGGAGGAGTTCAACAACTGCTTCTCTGCGGCAAATGGTCGAACACCCTGTATGCACTTTTTTGGTGCCACTTTGCAGCTCAGGGGAGTCAACCCAGTACTTCACCCATTGATAGATTCGTCTAAGAACGTTCTTGTCTATAATGGTGAGATCTTTGGGGGGATTGAGATTGCTAGTGAGGAGAATGACGGTGAAGTTCTAATGGGGTTGCTGGGAGGGTGTGAAGGTTGTGTGCCGAATGTTGTTTCTAGGATCAAGGGCCCTTGGGCTATCATCTATTGGCAGGAAAGTTCAAGAACATTATGGTTTGGGAGAGATGCATTTGGTCGCCGGAGTCTTCTTGTTCATTGGCCGACAGTGGAGGACTCTCGGTTTCTGTTATCATCAGTATCACCCGTTGCTTCAAATGAGCATTCTTCTGCTGAAAATGGAACAACCAAGCTTGGTTTTTGGGAGGAGCTGCCGTGTGGAATATACAGTGTATCCATAGATGCTCCCAATGGTGACGGGAGTTTGGTTGGTGAAGTGAAAAAGCATGAATGGACTAATGCCGTGCTAGAAGAACTGATAAAATGGGAGAGAACATCTGTCAAACCCAAACCCAAAGAGTTGCATATTTCACGTTGTGAGACTGTAAGAGGGCAACACGATATGAACTCACTTACTTCAGATATAGTGCCAATGCCATCTCAGTCAGGCAATATACAAGCTTCAATTTCAGTGCCAGCAGAGGCTGTGCTGATTGCTTTAAGGGAATCAGTTTTGCGGCGATCTTCACTGCACAGAATTTTTCAGGCAAGTGTGCATGATGTGGAAAAGAATCTTGTACCGGTGGCGGTTCTTTTTTCTGGTGGATTGGATTCTATGATAATTGCAGCATTGTTGCATGAGTGCCTAGATCCAAGCTATGAGATTGATCTACTTAATGTGAGCTTTGATGGTCAGTCTGCTCCAGATAGAATCTCTGCCAAGGCAGGAGTGAGCGAACTGAGAAGAATTGCACCCTTAAGAAAGTGGAAACTTGTGGAGATCGATGCTGATTTATCAAATTTGACCTTTGAAACAAAGCGTGTATTGTCTCTCATAAACCCTGCCAACACCTACATGGACCTAAATATAGGAATCGCGTTATGGCTGGCAGCTGGTGGGGATGGATGGGTTTATGAGGAAAATAGCAAATATAATGATGAGAATCGTCAAGGTGTTAAATACAGGTCAAAGGCTAGGATTGTCCTTGTTGGTTCAGGTGCAGATGAGCAATGTGCTGGCTATGGTAGGCACAGAACAAAATATAGAAGTGGAAGTTGGCTGCGACTGCATGAGGAAATGAAACTGGATATGCAAAGAATATGGAAAAGAAACCTTGGTAGAGATGATAGATGCATTGCTGACACTGGCAAGGAGGCAAGGTTTCCTTTTTTGGATGAAGATGTTATACGAACTTTATTGGGGATTCCTCTCTGGGAGGTTACCAACCTTGATCAACCAAGTGGAATAGGAGATAAGAAGATTTTGAGAGAGGTTGCGGAATTGCTTGGTTTACATGAAGCTGCTACTCTGCCTAAACGAGCTATACAGTTTGGTACCAGAATTGCGAGGGAGTCCAATAGAAAGAATTTCGGAAGTAACCGTGCTGCAAATCAGGCATCTGCGGGAAGTGTGGTGATTCACAAGCAGTTGGACACGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

632

Amino Acids

69.69

Weight (kDa)

6.07

Isoelectric Point (pI)

42.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	105 - 198	1.1e-07	Glutamine amidotransferase domain
Asn_synthase	PF00733	331 - 394	9.3e-06	Asparagine synthase
Asn_synthase	PF00733	502 - 593	1.6e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1790
AccB1I	GGYRCC	3 cut(s)	155, 272, 1790
AccBSI	CCGCTC	1 cut(s)	125
AccII	CGCG	1 cut(s)	1334
AciI	CCGC	7 cut(s)	6, 123, 236, 973, 1043, 1733, 1859
AclI	AACGTT	1 cut(s)	336
AclWI	GGATC	2 cut(s)	467, 1100
AcoI	YGGCCR	1 cut(s)	557
AcsI	RAATTY	3 cut(s)	993, 1255, 1822
AcuI	CTGAAG	1 cut(s)	858
AdeI	CACNNNGTG	1 cut(s)	833
AfaI	GTAC	3 cut(s)	306, 1035, 1792
AflII	CTTAAG	1 cut(s)	1213
AgeI	ACCGGT	1 cut(s)	1036
AgsI	TTSAA	5 cut(s)	220, 501, 612, 921, 1268
AhdI	GACNNNNNGTC	1 cut(s)	46
AjuI	GAANNNNNNNTTGG	2 cut(s)	1495, 1527
Alw26I	GTCTC	3 cut(s)	54, 830, 1290
AlwI	GGATC	2 cut(s)	467, 1100
AlwNI	CAGNNNCTG	1 cut(s)	941
AoxI	GGCC	3 cut(s)	139, 466, 557
ApaI	GGGCCC	2 cut(s)	143, 470
ApeKI	GCWGC	8 cut(s)	119, 283, 667, 1082, 1346, 1516, 1757, 1841
ApoI	RAATTY	3 cut(s)	993, 1255, 1822
AsiGI	ACCGGT	1 cut(s)	1036
Asp718I	GGTACC	1 cut(s)	1790
AspS9I	GGNCC	5 cut(s)	139, 140, 466, 467, 1315
AsuHPI	GGTGA	8 cut(s)	70, 302, 365, 410, 591, 722, 740, 1882
AvaII	GGWCC	1 cut(s)	1315
BaeGI	GKGCMC	2 cut(s)	143, 470
BanI	GGYRCC	3 cut(s)	155, 272, 1790
BanII	GRGCYC	2 cut(s)	143, 470
BarI	GAAGNNNNNNTAC	2 cut(s)	1626, 1658
BbsI	GAAGAC	1 cut(s)	536
BbvI	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
BccI	CCATC	4 cut(s)	901, 1139, 1352, 1356
BceAI	ACGGC	2 cut(s)	655, 743
BcgI	CGANNNNNNTGC	2 cut(s)	19, 53
BciVI	GTATCC	1 cut(s)	700
BclI	TGATCA	2 cut(s)	18, 1687
BcoDI	GTCTC	3 cut(s)	54, 830, 1290
BfaI	CTAG	6 cut(s)	95, 381, 456, 764, 1103, 1431
BfmI	CTRYAG	1 cut(s)	105
BfrI	CTTAAG	1 cut(s)	1213
BfuI	GTATCC	1 cut(s)	700
BglII	AGATCT	1 cut(s)	357
BmcAI	AGTACT	1 cut(s)	306
Bme18I	GGWCC	1 cut(s)	1315
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgT120I	GGNCC	5 cut(s)	139, 140, 466, 467, 1315
BmiI	GGNNCC	7 cut(s)	140, 141, 157, 187, 274, 468, 1792
BmrI	ACTGGG	1 cut(s)	296
BmsI	GCATC	5 cut(s)	514, 688, 1231, 1577, 1862
BmuI	ACTGGG	1 cut(s)	296
BpiI	GAAGAC	1 cut(s)	536
BpmI	CTGGAG	3 cut(s)	57, 222, 1143
Bpu10I	CCTNAGC	2 cut(s)	151, 287
Bsa29I	ATCGAT	1 cut(s)	1239
BsaBI	GATNNNNATC	1 cut(s)	1398
BsaJI	CCNNGG	3 cut(s)	470, 1176, 1570
BsaWI	WCCGGW	1 cut(s)	1036
BsaXI	ACNNNNNCTCC	6 cut(s)	55, 85, 656, 686, 1601, 1631
Bse118I	RCCGGY	1 cut(s)	1036
Bse1I	ACTGG	4 cut(s)	74, 302, 1545, 1606
Bse3DI	GCAATG	2 cut(s)	1472, 1590
Bse8I	GATNNNNATC	1 cut(s)	1398
BseCI	ATCGAT	1 cut(s)	1239
BseDI	CCNNGG	3 cut(s)	470, 1176, 1570
BseGI	GGATG	3 cut(s)	1363, 1367, 1636
BseJI	GATNNNNATC	1 cut(s)	1398
BseMI	GCAATG	2 cut(s)	1472, 1590
BseMII	CTCAG	3 cut(s)	301, 911, 1187
BseNI	ACTGG	4 cut(s)	74, 302, 1545, 1606
BseRI	GAGGAG	4 cut(s)	224, 227, 401, 677
BseSI	GKGCMC	2 cut(s)	143, 470
BseXI	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
BseYI	CCCAGC	1 cut(s)	418
BsgI	GTGCAG	2 cut(s)	971, 1476
Bsh1236I	CGCG	1 cut(s)	1334
BshFI	GGCC	3 cut(s)	141, 468, 559
BshNI	GGYRCC	3 cut(s)	155, 272, 1790
BshTI	ACCGGT	1 cut(s)	1036
BshVI	ATCGAT	1 cut(s)	1239
BsiSI	CCGG	3 cut(s)	27, 538, 1037
BsmAI	GTCTC	3 cut(s)	54, 830, 1290
BsmBI	CGTCTC	1 cut(s)	54
BsmI	GAATGC	2 cut(s)	30, 619
BsnI	GGCC	3 cut(s)	141, 468, 559
Bsp120I	GGGCCC	2 cut(s)	139, 466
Bsp1286I	GDGCHC	2 cut(s)	143, 470
Bsp143I	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
BspACI	CCGC	7 cut(s)	6, 123, 236, 973, 1043, 1733, 1859
BspANI	GGCC	3 cut(s)	141, 468, 559
BspCNI	CTCAG	3 cut(s)	300, 910, 1188
BspDI	ATCGAT	1 cut(s)	1239
BspFNI	CGCG	1 cut(s)	1334
BspLI	GGNNCC	7 cut(s)	140, 141, 157, 187, 274, 468, 1792
BspPI	GGATC	2 cut(s)	467, 1100
BspT107I	GGYRCC	3 cut(s)	155, 272, 1790
BspTI	CTTAAG	1 cut(s)	1213
BsrBI	CCGCTC	1 cut(s)	125
BsrDI	GCAATG	2 cut(s)	1472, 1590
BsrFI	RCCGGY	1 cut(s)	1036
BsrI	ACTGG	4 cut(s)	74, 302, 1545, 1606
BssAI	RCCGGY	1 cut(s)	1036
BssECI	CCNNGG	3 cut(s)	470, 1176, 1570
BssMI	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
BssT1I	CCWWGG	3 cut(s)	470, 1176, 1570
Bst4CI	ACNGT	6 cut(s)	398, 565, 686, 841, 1785, 1838
BstAFI	CTTAAG	1 cut(s)	1213
BstC8I	GCNNGC	3 cut(s)	933, 1344, 1474
BstDEI	CTNAG	6 cut(s)	151, 287, 330, 897, 1196, 1896
BstEII	GGTNACC	1 cut(s)	1675
BstF5I	GGATG	3 cut(s)	1363, 1367, 1636
BstFNI	CGCG	1 cut(s)	1334
BstKTI	GATC	8 cut(s)	21, 360, 462, 980, 1108, 1126, 1239, 1690
BstMAI	GTCTC	3 cut(s)	54, 830, 1290
BstMBI	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
BstMWI	GCNNNNNNNGC	4 cut(s)	116, 147, 1522, 1850
BstPI	GGTNACC	1 cut(s)	1675
BstSFI	CTRYAG	1 cut(s)	105
BstSLI	GKGCMC	2 cut(s)	143, 470
BstUI	CGCG	1 cut(s)	1334
BstV1I	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
BstV2I	GAAGAC	1 cut(s)	536
BstX2I	RGATCY	2 cut(s)	357, 1105
BstYI	RGATCY	2 cut(s)	357, 1105
Bsu15I	ATCGAT	1 cut(s)	1239
BsuI	GTATCC	1 cut(s)	700
BsuRI	GGCC	3 cut(s)	141, 468, 559
BsuTUI	ATCGAT	1 cut(s)	1239
BtsCI	GGATG	3 cut(s)	1363, 1367, 1636
BtsI	GCAGTG	1 cut(s)	983
BtsIMutI	CAGTG	5 cut(s)	570, 691, 933, 983, 1599
Cac8I	GCNNGC	3 cut(s)	933, 1344, 1474
CaiI	CAGNNNCTG	1 cut(s)	941
Cfr10I	RCCGGY	1 cut(s)	1036
Cfr13I	GGNCC	5 cut(s)	139, 140, 466, 467, 1315
ClaI	ATCGAT	1 cut(s)	1239
Csp6I	GTAC	3 cut(s)	305, 1034, 1791
CspAI	ACCGGT	1 cut(s)	1036
CviAII	CATG	6 cut(s)	743, 1013, 1094, 1312, 1526, 1751
CviQI	GTAC	3 cut(s)	305, 1034, 1791
DdeI	CTNAG	6 cut(s)	151, 287, 330, 897, 1196, 1896
DpnI	GATC	8 cut(s)	20, 359, 461, 979, 1107, 1125, 1238, 1689
DpnII	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
DraIII	CACNNNGTG	1 cut(s)	833
DriI	GACNNNNNGTC	1 cut(s)	46
EaeI	YGGCCR	1 cut(s)	557
Eam1105I	GACNNNNNGTC	1 cut(s)	46
Eco130I	CCWWGG	3 cut(s)	470, 1176, 1570
Eco24I	GRGCYC	2 cut(s)	143, 470
Eco47I	GGWCC	1 cut(s)	1315
Eco57I	CTGAAG	1 cut(s)	858
Eco91I	GGTNACC	1 cut(s)	1675
EcoO109I	RGGNCCY	3 cut(s)	139, 466, 467
EcoO65I	GGTNACC	1 cut(s)	1675
EcoT14I	CCWWGG	3 cut(s)	470, 1176, 1570
EcoT22I	ATGCAT	2 cut(s)	529, 1592
EcoT38I	GRGCYC	2 cut(s)	143, 470
ErhI	CCWWGG	3 cut(s)	470, 1176, 1570
Esp3I	CGTCTC	1 cut(s)	54
FaeI	CATG	6 cut(s)	746, 1016, 1097, 1315, 1529, 1754
FalI	AAGNNNNNCTT	2 cut(s)	1606, 1638
FatI	CATG	6 cut(s)	742, 1012, 1093, 1311, 1525, 1750
FauI	CCCGC	1 cut(s)	1852
FbaI	TGATCA	2 cut(s)	18, 1687
FokI	GGATG	3 cut(s)	1370, 1374, 1643
FriOI	GRGCYC	2 cut(s)	143, 470
FspBI	CTAG	6 cut(s)	95, 381, 456, 764, 1103, 1431
GsaI	CCCAGC	1 cut(s)	422
GsuI	CTGGAG	3 cut(s)	57, 222, 1143
HaeIII	GGCC	3 cut(s)	141, 468, 559
HapII	CCGG	3 cut(s)	27, 538, 1037
Hin1II	CATG	6 cut(s)	746, 1016, 1097, 1315, 1529, 1754
HincII	GTYRAC	1 cut(s)	298
HindII	GTYRAC	1 cut(s)	298
HindIII	AAGCTT	3 cut(s)	164, 647, 915
HpaII	CCGG	3 cut(s)	27, 538, 1037
HphI	GGTGA	8 cut(s)	70, 302, 365, 410, 591, 722, 740, 1882
Hpy166II	GTNNAC	3 cut(s)	102, 298, 1748
Hpy188I	TCNGA	5 cut(s)	18, 88, 190, 877, 1829
Hpy188III	TCNNGA	4 cut(s)	59, 201, 501, 1160
Hpy8I	GTNNAC	3 cut(s)	102, 298, 1748
Hpy99I	CGWCG	1 cut(s)	135
HpyAV	CCTTC	1 cut(s)	425
HpyCH4III	ACNGT	6 cut(s)	398, 565, 686, 841, 1785, 1838
HpyCH4IV	ACGT	2 cut(s)	336, 829
HpyF10VI	GCNNNNNNNGC	4 cut(s)	116, 147, 1522, 1850
HpyF3I	CTNAG	6 cut(s)	151, 287, 330, 897, 1196, 1896
HpySE526I	ACGT	2 cut(s)	336, 829
Hsp92II	CATG	6 cut(s)	746, 1016, 1097, 1315, 1529, 1754
KpnI	GGTACC	1 cut(s)	1794
Ksp22I	TGATCA	2 cut(s)	18, 1687
Kzo9I	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
LmnI	GCTCC	4 cut(s)	191, 664, 706, 1162
Lsp1109I	GCAGC	8 cut(s)	131, 295, 654, 1094, 1358, 1503, 1744, 1828
LweI	GCATC	5 cut(s)	514, 688, 1231, 1577, 1862
MaeI	CTAG	6 cut(s)	95, 381, 456, 764, 1103, 1431
MaeII	ACGT	2 cut(s)	336, 829
MaeIII	GTNAC	4 cut(s)	76, 710, 1675, 1832
MalI	GATC	8 cut(s)	20, 359, 461, 979, 1107, 1125, 1238, 1689
MbiI	CCGCTC	1 cut(s)	125
MboI	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
MboII	GAAGA	9 cut(s)	161, 164, 536, 615, 779, 972, 1212, 1646, 1726
MflI	RGATCY	2 cut(s)	357, 1105
MhlI	GDGCHC	2 cut(s)	143, 470
MluCI	AATT	8 cut(s)	921, 993, 1077, 1203, 1255, 1736, 1797, 1822
MlyI	GAGTC	4 cut(s)	303, 550, 566, 1817
MmeI	TCCRAC	2 cut(s)	111, 1866
Mph1103I	ATGCAT	2 cut(s)	529, 1592
MseI	TTAA	4 cut(s)	956, 1131, 1214, 1413
MslI	CAYNNNNRTG	1 cut(s)	1310
MspA1I	CMGCKG	1 cut(s)	1349
MspCI	CTTAAG	1 cut(s)	1213
MspI	CCGG	3 cut(s)	27, 538, 1037
Mva1269I	GAATGC	2 cut(s)	30, 619
MvnI	CGCG	1 cut(s)	1334
MwoI	GCNNNNNNNGC	4 cut(s)	116, 147, 1522, 1850
NdeII	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
NlaIII	CATG	6 cut(s)	746, 1016, 1097, 1315, 1529, 1754
NlaIV	GGNNCC	7 cut(s)	140, 141, 157, 187, 274, 468, 1792
NmuCI	GTSAC	2 cut(s)	76, 710
NsiI	ATGCAT	2 cut(s)	529, 1592
PctI	GAATGC	2 cut(s)	30, 619
PinAI	ACCGGT	1 cut(s)	1036
PleI	GAGTC	4 cut(s)	302, 549, 566, 1816
PpsI	GAGTC	4 cut(s)	302, 549, 566, 1816
Psp1406I	AACGTT	1 cut(s)	336
PspEI	GGTNACC	1 cut(s)	1675
PspFI	CCCAGC	1 cut(s)	418
PspN4I	GGNNCC	7 cut(s)	140, 141, 157, 187, 274, 468, 1792
PspOMI	GGGCCC	2 cut(s)	139, 466
PspPI	GGNCC	5 cut(s)	139, 140, 466, 467, 1315
PstNI	CAGNNNCTG	1 cut(s)	941
PsuI	RGATCY	2 cut(s)	357, 1105
PvuII	CAGCTG	1 cut(s)	1349
RsaI	GTAC	3 cut(s)	306, 1035, 1792
RsaNI	GTAC	3 cut(s)	305, 1034, 1791
RseI	CAYNNNNRTG	1 cut(s)	1310
SaqAI	TTAA	4 cut(s)	956, 1131, 1214, 1413
Sau3AI	GATC	8 cut(s)	18, 357, 459, 977, 1105, 1123, 1236, 1687
Sau96I	GGNCC	5 cut(s)	139, 140, 466, 467, 1315
ScaI	AGTACT	1 cut(s)	306
SchI	GAGTC	4 cut(s)	303, 550, 566, 1817
SduI	GDGCHC	2 cut(s)	143, 470
SfaNI	GCATC	5 cut(s)	514, 688, 1231, 1577, 1862
SfcI	CTRYAG	1 cut(s)	105
SinI	GGWCC	1 cut(s)	1315
SmiMI	CAYNNNNRTG	1 cut(s)	1310
SmlI	CTYRAG	1 cut(s)	1213
SmoI	CTYRAG	1 cut(s)	1213
Sse9I	AATT	8 cut(s)	921, 993, 1077, 1203, 1255, 1736, 1797, 1822
SsiI	CCGC	7 cut(s)	6, 123, 236, 973, 1043, 1733, 1859
SspMI	CTAG	6 cut(s)	95, 381, 456, 764, 1103, 1431
StyI	CCWWGG	3 cut(s)	470, 1176, 1570
TaaI	ACNGT	6 cut(s)	398, 565, 686, 841, 1785, 1838
TaiI	ACGT	2 cut(s)	339, 832
TaqI	TCGA	4 cut(s)	39, 60, 247, 1239
TasI	AATT	8 cut(s)	921, 993, 1077, 1203, 1255, 1736, 1797, 1822
TatI	WGTACW	1 cut(s)	304
TauI	GCSGC	3 cut(s)	125, 239, 976
Tru1I	TTAA	4 cut(s)	956, 1131, 1214, 1413
Tru9I	TTAA	4 cut(s)	956, 1131, 1214, 1413
TscAI	CASTG	5 cut(s)	570, 691, 933, 990, 1606
TseFI	GTSAC	2 cut(s)	76, 710
TseI	GCWGC	8 cut(s)	119, 283, 667, 1082, 1346, 1516, 1757, 1841
Tsp45I	GTSAC	2 cut(s)	76, 710
TspDTI	ATGAA	6 cut(s)	542, 759, 875, 1550, 1647, 1767
TspGWI	ACGGA	1 cut(s)	148
TspRI	CASTG	5 cut(s)	570, 691, 933, 990, 1606
Vha464I	CTTAAG	1 cut(s)	1213
VpaK11BI	GGWCC	1 cut(s)	1315
XapI	RAATTY	3 cut(s)	993, 1255, 1822
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1309
XspI	CTAG	6 cut(s)	95, 381, 456, 764, 1103, 1431
ZrmI	AGTACT	1 cut(s)	306
Zsp2I	ATGCAT	2 cut(s)	529, 1592

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.