Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

29802195 .. 29806402

Gene Structure 4208 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g29880.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGTGCGGAATCGCTCTGATCATCTACGGCGTTCGCTTCGACGTATCGTCTCTGCTTCTCGGCACCACACTTCCGGTCACCAACTCCGACCAACTACAGTTCACTACCGCCGACCTCGCCGCCGCTCTGCGACGGCGGGGCCCAGATAGCCTCGGCACCAAGAAGCTTCTTCTTCATTCGTCAGGCTCCAAAGTCGCGTCCTTTATTGATGGAGGAGAGGAGGAGTTACCTTGTATGGACTTTTTTGGCGCCACTTTGCAGCTCAGGGGAGTAAACCCAGTAGCTCAGCCATTGGTGGATTTGAATAAGAATGTTATGGTTTATAATGGTGAAATCTTTGGGGGAATTGAGATTGGTGGTGAGGAAAATGATGGTGAAGTTCTTATGAGGTCGCTGGGAGAGTGTGGAGGTTCTGTGCCGAATGTTGTTTCTAGGATCAAGGGACCTTGGGCTCTCATCTATTGGCAGGAAAGTACCAGAACATTATGGTTTGGGAGAGATGCATTTGGTCGCCGGAGTCTGCTTGTTCACTGGCCGACAGTGGAGGACTCCCGGTTTTTGTTATCGTCGGTATCGCCTGTTGCTTCAAATGAGCGTTCTTCTGCTGAAAATGGAACTACCAAGCGTGATTTTTGGGAAGAGCTCCCGTGTGGAATATACAGTGTAACCATAGATGCTCCTGATTGTGATGGGTTTTTGGTTGGTGAAGTGAAAAAGCATGAATGGACTAATGCCATGCTAGAAGAACTGATAAAATGGGAGAGAACATCTATTGAACCCAAACCTGAAGAGTTGCATATTTCACATTGCGAGACTGTAAGAGGGCAACCTGAAGAGTTACTTGCTTCAGATGTAATGCCAATGCCATCTAAATCAGGCAATATACAAGCTTCAATATCAGTGCCAGCAGAGACTGTGCTGATTGCTTTAAGGGAGTCAGTTTTGTGGCGATCTTCACTGCACAGAATTTTTCAGGCAGGTATGGATGATGTGGAAAAGAATCTTGTACCGGTGGCAGTTCTTTTTTCTGGTGGATTGGATTCTATGATAATTGCAGCATTGTTGCATGAGTGCCTAGATCCAAGCTATGAGATTGATCTACTTAATGTGAGCTTTGATGGTCAGTCTGCGCCAGATAGGATCTCTGCCAAGGCAGGAGTAAGCGAACTGAGAAGAATTGCACCTTTAAGAAAGTGGAAGCTTGTGGAAATTGATGCTGATTTATCAAATTTGACCTTTGAATCAAAGCGTGTATTGTCTCTCATAAACCCTGCCAACACCTATATGGACCTAAATATAGGAATTGCTTTATGGCTGGCAGCTGGTGGTGATGGATGGGTGTATGAGGAAAATAGCAAATATAATGATGAGAATTGTCGAGGTGTTAGATACAAGTCAAATGCTAGGATTGTCCTTGTTGGTTCAGGTGCAGATGAGCAATGTGCTGGCTATGGTAGGCACAGAACAAAATATAGAAGTGGCAGTTGGCTGCGGCTGCATGAGGAAATGAAACTGGATATGCAAAGAATATGGAAAAGAAACCTTGGTAGAGATGATAGATGCATTGCTGACACTGGCAAGGAGGCAAGGTTTCCTTTCTTGGATGAAGATGTTATACGAACTTTATTGGGGATTCCTCTCTGGGAGATTACCAACCTTGATCAACCAAGTGGAATAGGAGATAAGAAGATTCTGAGAGAGGTTGCGGAATTGCTTGGTTTACATGAAGCTGCTACTCTGCCTAAACGAGCTATCCAGTTTGGTACTAGAATTGCAAGGGAATCCAATAGAAAGAATTTCGGAAGTAACCGTGCAGCAAATCAGGCATCTGCGGGAAGTGCAGTGATTCACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

619

Amino Acids

68.4

Weight (kDa)

5.52

Isoelectric Point (pI)

45.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_7	PF13537	95 - 190	1e-08	Glutamine amidotransferase domain
Asn_synthase	PF00733	494 - 582	2.1e-08	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	324
Acc36I	ACCTGC	1 cut(s)	968
AccB1I	GGYRCC	3 cut(s)	62, 155, 248
AccBSI	CCGCTC	1 cut(s)	125
AccII	CGCG	1 cut(s)	197
AciI	CCGC	8 cut(s)	6, 108, 120, 123, 136, 1492, 1706, 1832
AclWI	GGATC	3 cut(s)	443, 1073, 1148
AcoI	YGGCCR	1 cut(s)	533
AcsI	RAATTY	3 cut(s)	966, 1228, 1795
AcuI	CTGAAG	3 cut(s)	807, 831, 852
AcyI	GRCGYC	1 cut(s)	249
AfaI	GTAC	3 cut(s)	475, 1008, 1765
AgeI	ACCGGT	1 cut(s)	1009
AgsI	TTSAA	5 cut(s)	304, 588, 776, 894, 1241
AhdI	GACNNNNNGTC	1 cut(s)	46
AjuI	GAANNNNNNNTTGG	2 cut(s)	1468, 1500
Alw21I	GWGCWC	1 cut(s)	645
Alw26I	GTCTC	4 cut(s)	54, 806, 905, 1263
AlwI	GGATC	3 cut(s)	443, 1073, 1148
AlwNI	CAGNNNCTG	1 cut(s)	914
AoxI	GGCC	2 cut(s)	139, 533
ApaI	GGGCCC	1 cut(s)	143
ApeKI	GCWGC	7 cut(s)	259, 1055, 1319, 1489, 1495, 1730, 1814
ApoI	RAATTY	3 cut(s)	966, 1228, 1795
AsiGI	ACCGGT	1 cut(s)	1009
AspLEI	GCGC	2 cut(s)	251, 1132
AspS9I	GGNCC	4 cut(s)	139, 140, 443, 1288
AsuC2I	CCSGG	1 cut(s)	553
AsuHPI	GGTGA	6 cut(s)	70, 341, 371, 386, 716, 1340
AvaII	GGWCC	2 cut(s)	443, 1288
BaeGI	GKGCMC	1 cut(s)	143
BanI	GGYRCC	3 cut(s)	62, 155, 248
BanII	GRGCYC	3 cut(s)	143, 454, 645
BarI	GAAGNNNNNNTAC	2 cut(s)	1599, 1631
Bbv12I	GWGCWC	1 cut(s)	645
BbvI	GCAGC	7 cut(s)	271, 1067, 1331, 1476, 1482, 1717, 1826
BccI	CCATC	7 cut(s)	203, 365, 683, 874, 1112, 1325, 1329
BceAI	ACGGC	2 cut(s)	43, 149
BclI	TGATCA	2 cut(s)	18, 1660
BcnI	CCSGG	1 cut(s)	553
BcoDI	GTCTC	4 cut(s)	54, 806, 905, 1263
BfaI	CTAG	5 cut(s)	432, 740, 1076, 1404, 1767
BfmI	CTRYAG	1 cut(s)	95
BfoI	RGCGCY	1 cut(s)	252
BfuAI	ACCTGC	1 cut(s)	968
BlpI	GCTNAGC	1 cut(s)	285
Bme1390I	CCNGG	1 cut(s)	553
Bme18I	GGWCC	2 cut(s)	443, 1288
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgT120I	GGNCC	4 cut(s)	139, 140, 443, 1288
BmiI	GGNNCC	7 cut(s)	64, 140, 141, 157, 187, 250, 444
BmrFI	CCNGG	1 cut(s)	553
BmrI	ACTGGG	1 cut(s)	272
BmsI	GCATC	5 cut(s)	490, 664, 1204, 1550, 1835
BmuI	ACTGGG	1 cut(s)	272
Bpu10I	CCTNAGC	1 cut(s)	263
Bpu1102I	GCTNAGC	1 cut(s)	285
BpuMI	CCSGG	1 cut(s)	553
BsaHI	GRCGYC	1 cut(s)	249
BsaJI	CCNNGG	4 cut(s)	151, 446, 1149, 1543
BsaWI	WCCGGW	2 cut(s)	73, 1009
BsaXI	ACNNNNNCTCC	4 cut(s)	399, 429, 1574, 1604
Bse118I	RCCGGY	1 cut(s)	1009
Bse1I	ACTGG	5 cut(s)	278, 536, 1518, 1579, 1756
Bse3DI	GCAATG	3 cut(s)	805, 1445, 1563
BseDI	CCNNGG	4 cut(s)	151, 446, 1149, 1543
BseGI	GGATG	3 cut(s)	991, 1340, 1609
BseMI	GCAATG	3 cut(s)	805, 1445, 1563
BseMII	CTCAG	4 cut(s)	277, 299, 1160, 1685
BseNI	ACTGG	5 cut(s)	278, 536, 1518, 1579, 1756
BseRI	GAGGAG	3 cut(s)	228, 233, 236
BseSI	GKGCMC	1 cut(s)	143
BseXI	GCAGC	7 cut(s)	271, 1067, 1331, 1476, 1482, 1717, 1826
BseYI	CCCAGC	1 cut(s)	394
BsgI	GTGCAG	3 cut(s)	944, 1449, 1833
Bsh1236I	CGCG	1 cut(s)	197
BshFI	GGCC	2 cut(s)	141, 535
BshNI	GGYRCC	3 cut(s)	62, 155, 248
BshTI	ACCGGT	1 cut(s)	1009
BsiHKAI	GWGCWC	1 cut(s)	645
BsiSI	CCGG	4 cut(s)	74, 514, 553, 1010
BslFI	GGGAC	1 cut(s)	456
BsmAI	GTCTC	4 cut(s)	54, 806, 905, 1263
BsmBI	CGTCTC	1 cut(s)	54
BsmFI	GGGAC	1 cut(s)	456
BsnI	GGCC	2 cut(s)	141, 535
Bsp120I	GGGCCC	1 cut(s)	139
Bsp1286I	GDGCHC	3 cut(s)	143, 454, 645
Bsp143I	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
Bsp1720I	GCTNAGC	1 cut(s)	285
BspACI	CCGC	8 cut(s)	6, 108, 120, 123, 136, 1492, 1706, 1832
BspANI	GGCC	2 cut(s)	141, 535
BspCNI	CTCAG	4 cut(s)	276, 298, 1161, 1686
BspFNI	CGCG	1 cut(s)	197
BspLI	GGNNCC	7 cut(s)	64, 140, 141, 157, 187, 250, 444
BspMI	ACCTGC	1 cut(s)	968
BspPI	GGATC	3 cut(s)	443, 1073, 1148
BspQI	GCTCTTC	1 cut(s)	633
BspT107I	GGYRCC	3 cut(s)	62, 155, 248
BsrBI	CCGCTC	1 cut(s)	125
BsrDI	GCAATG	3 cut(s)	805, 1445, 1563
BsrFI	RCCGGY	1 cut(s)	1009
BsrI	ACTGG	5 cut(s)	278, 536, 1518, 1579, 1756
BssAI	RCCGGY	1 cut(s)	1009
BssECI	CCNNGG	4 cut(s)	151, 446, 1149, 1543
BssMI	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
BssNI	GRCGYC	1 cut(s)	249
BssT1I	CCWWGG	3 cut(s)	446, 1149, 1543
Bst4CI	ACNGT	6 cut(s)	99, 541, 662, 817, 916, 1811
Bst6I	CTCTTC	3 cut(s)	633, 783, 828
BstACI	GRCGYC	1 cut(s)	249
BstC8I	GCNNGC	3 cut(s)	906, 1317, 1447
BstDEI	CTNAG	4 cut(s)	263, 285, 1169, 1694
BstEII	GGTNACC	1 cut(s)	76
BstF5I	GGATG	3 cut(s)	991, 1340, 1609
BstFNI	CGCG	1 cut(s)	197
BstH2I	RGCGCY	1 cut(s)	252
BstHHI	GCGC	2 cut(s)	251, 1132
BstKTI	GATC	7 cut(s)	21, 438, 953, 1081, 1099, 1143, 1663
BstMAI	GTCTC	4 cut(s)	54, 806, 905, 1263
BstMBI	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
BstMWI	GCNNNNNNNGC	5 cut(s)	116, 147, 1495, 1823, 1838
BstPI	GGTNACC	1 cut(s)	76
BstSCI	CCNGG	1 cut(s)	551
BstSFI	CTRYAG	1 cut(s)	95
BstSLI	GKGCMC	1 cut(s)	143
BstUI	CGCG	1 cut(s)	197
BstV1I	GCAGC	7 cut(s)	271, 1067, 1331, 1476, 1482, 1717, 1826
BstX2I	RGATCY	2 cut(s)	1078, 1140
BstYI	RGATCY	2 cut(s)	1078, 1140
BsuRI	GGCC	2 cut(s)	141, 535
BtsCI	GGATG	3 cut(s)	991, 1340, 1609
BtsI	GCAGTG	2 cut(s)	956, 1848
BtsIMutI	CAGTG	7 cut(s)	529, 546, 667, 906, 956, 1572, 1848
BveI	ACCTGC	1 cut(s)	968
Cac8I	GCNNGC	3 cut(s)	906, 1317, 1447
CaiI	CAGNNNCTG	1 cut(s)	914
CfoI	GCGC	2 cut(s)	251, 1132
Cfr10I	RCCGGY	1 cut(s)	1009
Cfr13I	GGNCC	4 cut(s)	139, 140, 443, 1288
CseI	GACGC	1 cut(s)	186
Csp6I	GTAC	3 cut(s)	474, 1007, 1764
CspAI	ACCGGT	1 cut(s)	1009
CviAII	CATG	5 cut(s)	719, 736, 1067, 1499, 1724
CviQI	GTAC	3 cut(s)	474, 1007, 1764
DdeI	CTNAG	4 cut(s)	263, 285, 1169, 1694
DinI	GGCGCC	1 cut(s)	250
DpnI	GATC	7 cut(s)	20, 437, 952, 1080, 1098, 1142, 1662
DpnII	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
DriI	GACNNNNNGTC	1 cut(s)	46
EaeI	YGGCCR	1 cut(s)	533
Eam1104I	CTCTTC	3 cut(s)	633, 783, 828
Eam1105I	GACNNNNNGTC	1 cut(s)	46
EarI	CTCTTC	3 cut(s)	633, 783, 828
Ecl136II	GAGCTC	1 cut(s)	643
Eco130I	CCWWGG	3 cut(s)	446, 1149, 1543
Eco24I	GRGCYC	3 cut(s)	143, 454, 645
Eco47I	GGWCC	2 cut(s)	443, 1288
Eco53kI	GAGCTC	1 cut(s)	643
Eco57I	CTGAAG	3 cut(s)	807, 831, 852
Eco91I	GGTNACC	1 cut(s)	76
EcoICRI	GAGCTC	1 cut(s)	643
EcoO109I	RGGNCCY	2 cut(s)	139, 443
EcoO65I	GGTNACC	1 cut(s)	76
EcoT14I	CCWWGG	3 cut(s)	446, 1149, 1543
EcoT22I	ATGCAT	2 cut(s)	505, 1565
EcoT38I	GRGCYC	3 cut(s)	143, 454, 645
EgeI	GGCGCC	1 cut(s)	250
EheI	GGCGCC	1 cut(s)	250
ErhI	CCWWGG	3 cut(s)	446, 1149, 1543
Esp3I	CGTCTC	1 cut(s)	54
FaeI	CATG	5 cut(s)	722, 739, 1070, 1502, 1727
FalI	AAGNNNNNCTT	4 cut(s)	825, 857, 1579, 1611
FaqI	GGGAC	1 cut(s)	456
FatI	CATG	5 cut(s)	718, 735, 1066, 1498, 1723
FauI	CCCGC	2 cut(s)	129, 1825
FbaI	TGATCA	2 cut(s)	18, 1660
FokI	GGATG	3 cut(s)	998, 1347, 1616
FriOI	GRGCYC	3 cut(s)	143, 454, 645
FspBI	CTAG	5 cut(s)	432, 740, 1076, 1404, 1767
GlaI	GCGC	2 cut(s)	250, 1131
GsaI	CCCAGC	1 cut(s)	398
HaeII	RGCGCY	1 cut(s)	252
HaeIII	GGCC	2 cut(s)	141, 535
HapII	CCGG	4 cut(s)	74, 514, 553, 1010
HgaI	GACGC	1 cut(s)	186
HhaI	GCGC	2 cut(s)	251, 1132
Hin1I	GRCGYC	1 cut(s)	249
Hin1II	CATG	5 cut(s)	722, 739, 1070, 1502, 1727
Hin6I	GCGC	2 cut(s)	249, 1130
HinP1I	GCGC	2 cut(s)	249, 1130
HindIII	AAGCTT	3 cut(s)	164, 888, 1199
HpaII	CCGG	4 cut(s)	74, 514, 553, 1010
HphI	GGTGA	6 cut(s)	70, 341, 371, 386, 716, 1340
Hpy166II	GTNNAC	4 cut(s)	102, 274, 529, 1721
Hpy188I	TCNGA	5 cut(s)	18, 88, 850, 1695, 1802
Hpy188III	TCNNGA	1 cut(s)	680
Hpy8I	GTNNAC	4 cut(s)	102, 274, 529, 1721
Hpy99I	CGWCG	3 cut(s)	44, 135, 571
HpyCH4III	ACNGT	6 cut(s)	99, 541, 662, 817, 916, 1811
HpyCH4IV	ACGT	1 cut(s)	42
HpyF10VI	GCNNNNNNNGC	5 cut(s)	116, 147, 1495, 1823, 1838
HpyF3I	CTNAG	4 cut(s)	263, 285, 1169, 1694
HpySE526I	ACGT	1 cut(s)	42
Hsp92I	GRCGYC	1 cut(s)	249
Hsp92II	CATG	5 cut(s)	722, 739, 1070, 1502, 1727
HspAI	GCGC	2 cut(s)	249, 1130
KasI	GGCGCC	1 cut(s)	248
Ksp22I	TGATCA	2 cut(s)	18, 1660
Kzo9I	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
LguI	GCTCTTC	1 cut(s)	633
LmnI	GCTCC	3 cut(s)	191, 648, 682
Lsp1109I	GCAGC	7 cut(s)	271, 1067, 1331, 1476, 1482, 1717, 1826
LweI	GCATC	5 cut(s)	490, 664, 1204, 1550, 1835
MaeI	CTAG	5 cut(s)	432, 740, 1076, 1404, 1767
MaeII	ACGT	1 cut(s)	42
MaeIII	GTNAC	5 cut(s)	76, 225, 664, 837, 1805
MalI	GATC	7 cut(s)	20, 437, 952, 1080, 1098, 1142, 1662
MbiI	CCGCTC	1 cut(s)	125
MboI	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
MflI	RGATCY	2 cut(s)	1078, 1140
MhlI	GDGCHC	3 cut(s)	143, 454, 645
Mly113I	GGCGCC	1 cut(s)	249
MlyI	GAGTC	3 cut(s)	526, 542, 944
MmeI	TCCRAC	1 cut(s)	111
Mph1103I	ATGCAT	2 cut(s)	505, 1565
MseI	TTAA	3 cut(s)	929, 1104, 1187
MslI	CAYNNNNRTG	1 cut(s)	1283
MspA1I	CMGCKG	1 cut(s)	1322
MspI	CCGG	4 cut(s)	74, 514, 553, 1010
MspR9I	CCNGG	1 cut(s)	553
MvnI	CGCG	1 cut(s)	197
MwoI	GCNNNNNNNGC	5 cut(s)	116, 147, 1495, 1823, 1838
NarI	GGCGCC	1 cut(s)	249
NciI	CCSGG	1 cut(s)	553
NdeII	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
NlaIII	CATG	5 cut(s)	722, 739, 1070, 1502, 1727
NlaIV	GGNNCC	7 cut(s)	64, 140, 141, 157, 187, 250, 444
NmeAIII	GCCGAG	2 cut(s)	39, 132
NmuCI	GTSAC	1 cut(s)	76
NsiI	ATGCAT	2 cut(s)	505, 1565
PciSI	GCTCTTC	1 cut(s)	633
PfeI	GAWTC	8 cut(s)	9, 1000, 1040, 1241, 1633, 1690, 1781, 1846
PinAI	ACCGGT	1 cut(s)	1009
PleI	GAGTC	3 cut(s)	525, 542, 943
PluTI	GGCGCC	1 cut(s)	252
PpsI	GAGTC	3 cut(s)	525, 542, 943
PpuMI	RGGWCCY	1 cut(s)	443
PsiI	TTATAA	1 cut(s)	324
Psp124BI	GAGCTC	1 cut(s)	645
Psp5II	RGGWCCY	1 cut(s)	443
PspEI	GGTNACC	1 cut(s)	76
PspFI	CCCAGC	1 cut(s)	394
PspN4I	GGNNCC	7 cut(s)	64, 140, 141, 157, 187, 250, 444
PspOMI	GGGCCC	1 cut(s)	139
PspPI	GGNCC	4 cut(s)	139, 140, 443, 1288
PspPPI	RGGWCCY	1 cut(s)	443
PstNI	CAGNNNCTG	1 cut(s)	914
PsuI	RGATCY	2 cut(s)	1078, 1140
PvuII	CAGCTG	1 cut(s)	1322
RsaI	GTAC	3 cut(s)	475, 1008, 1765
RsaNI	GTAC	3 cut(s)	474, 1007, 1764
RseI	CAYNNNNRTG	1 cut(s)	1283
SacI	GAGCTC	1 cut(s)	645
SapI	GCTCTTC	1 cut(s)	633
SaqAI	TTAA	3 cut(s)	929, 1104, 1187
Sau3AI	GATC	7 cut(s)	18, 435, 950, 1078, 1096, 1140, 1660
Sau96I	GGNCC	4 cut(s)	139, 140, 443, 1288
SchI	GAGTC	3 cut(s)	526, 542, 944
ScrFI	CCNGG	1 cut(s)	553
SduI	GDGCHC	3 cut(s)	143, 454, 645
SfaNI	GCATC	5 cut(s)	490, 664, 1204, 1550, 1835
SfcI	CTRYAG	1 cut(s)	95
SfoI	GGCGCC	1 cut(s)	250
SinI	GGWCC	2 cut(s)	443, 1288
SmiMI	CAYNNNNRTG	1 cut(s)	1283
SsiI	CCGC	8 cut(s)	6, 108, 120, 123, 136, 1492, 1706, 1832
SspDI	GGCGCC	1 cut(s)	248
SspMI	CTAG	5 cut(s)	432, 740, 1076, 1404, 1767
SstI	GAGCTC	1 cut(s)	645
StyD4I	CCNGG	1 cut(s)	551
StyI	CCWWGG	3 cut(s)	446, 1149, 1543
TaaI	ACNGT	6 cut(s)	99, 541, 662, 817, 916, 1811
TaiI	ACGT	1 cut(s)	45
TaqI	TCGA	2 cut(s)	39, 1378
TauI	GCSGC	3 cut(s)	122, 125, 1495
TfiI	GAWTC	8 cut(s)	9, 1000, 1040, 1241, 1633, 1690, 1781, 1846
Tru1I	TTAA	3 cut(s)	929, 1104, 1187
Tru9I	TTAA	3 cut(s)	929, 1104, 1187
TscAI	CASTG	7 cut(s)	536, 546, 667, 906, 963, 1579, 1848
TseFI	GTSAC	1 cut(s)	76
TseI	GCWGC	7 cut(s)	259, 1055, 1319, 1489, 1495, 1730, 1814
Tsp45I	GTSAC	1 cut(s)	76
TspDTI	ATGAA	5 cut(s)	164, 735, 1523, 1620, 1740
TspRI	CASTG	7 cut(s)	536, 546, 667, 906, 963, 1579, 1848
VpaK11BI	GGWCC	2 cut(s)	443, 1288
XapI	RAATTY	3 cut(s)	966, 1228, 1795
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1282
XspI	CTAG	5 cut(s)	432, 740, 1076, 1404, 1767
Zsp2I	ATGCAT	2 cut(s)	505, 1565

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.