AT2G14830

Regulator of Vps4 activity in the MVB pathway protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013512
Physical Location & Seq
Forward (+)
6364378 .. 6365818
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G14830.1

Sequence Viewer

Length: 1155 bp
ATGTTTTGTTTTTTATTCGGATGGCGAAGAACCTCCAAGTGTAAGAGTGTGGTCAAACAGCTTCAGTGTCGTCTAAACCTACTCAAGAACAAGAAATACGCTATTTCTTCACATTTACGTAACGACATAGCTCAGTTACTACGTATTGGTGAACGAGACCGTGCTCTTCATCGTGCTCAACAACTCTTCCTTGATGAGAATCTAATGTCTCTCTACCACTTGTTGCTTCACTTCTCCGACATAATCCTTCTCAACCTCTCTTACATCCGGAGACGTAGAGACCTTCCTGATGGCATCAATGAAGCTGTTTCAACACTTGTCTTTGCCTCTGCGAGATGTGGAGACTTGCCTGAGCTACGAGCCTTAAGGGTACTTTTTGGCAAGCGTTATGGGAATCACTTTGTGGACACGGCTCTTAATCTTCTGCCTGGTAATTGTGTCAACCCTCAGGTTATAGAGAAACTCTCCATAATCTCAGTATCGGATGATGCCAAGTCCAAACTATTAGGTGAAATAGTGGAAGAGTATAACCTTCGTCTAGAGGTGTTGGCAATCGAATACACTCCCGAATTTCACAAACAGGTACTAAAAAGCGAGAAGGCAGAAGAAGAGAAAGAAGTCATGAGTTCCAATTCTGCTCAGCCGTACTGTTCATCACAAAAGGCTGAGAGTGAAGCAGAAGTGTATAAGTTTACTCTCACGGATGCGGATATAGAGGAAAACCAAGAACAAGAAAGAAAAGTATGTGATGAGGAAGATGATTGCATAGAGGAAGAAGTGGTGGAAGAAGATCAGAGTGTATTCAGATTTAGAGAGAGTACTGAAGATGAGAGAAAAGAGAGAAAGAGGTTAAGGAGGAAACCAAGATCAACTTCATCATCAAGCAGTCCTATCGCGAAAGACGTGGATTGTTGGAGGTACTATTACAAGGGAAAGAGAAGTCGTCAAAGGAAAGAGTGTGGTAAATGTTGTTACCATATAGTGTACAACGTGTTTACAATGTTGCCGGATCAAAAGGAAAGCGAAGAAGGAGAAAGAAGTTTCAAAAAAGCTATGCATGTTCATCCTAAGCTTCCCGATTATGATCAGATTGTTGCTCACTTCACTGCTCTTAGGAAACAACAACAACAAAAACAAAAGCATATGCGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

384

Amino Acids

45.04

Weight (kDa)

8.33

Isoelectric Point (pI)

65.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 178 4.1e-45 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 896
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 1 cut(s) 707
AclWI GGATC 1 cut(s) 1017
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 2 cut(s) 47, 843
AdeI CACNNNGTG 1 cut(s) 403
AfaI GTAC 6 cut(s) 372, 585, 647, 820, 920, 986
AflII CTTAAG 1 cut(s) 364
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 2 cut(s) 312, 1045
AjiI CACGTC 1 cut(s) 904
AjnI CCWGG 1 cut(s) 427
AjuI GAANNNNNNNTTGG 2 cut(s) 856, 888
AluBI AGCT 6 cut(s) 61, 131, 305, 355, 1052, 1072
AluI AGCT 6 cut(s) 61, 131, 305, 355, 1052, 1072
Alw21I GWGCWC 2 cut(s) 166, 178
Alw26I GTCTC 5 cut(s) 150, 213, 265, 273, 336
AlwI GGATC 1 cut(s) 1017
Aor13HI TCCGGA 1 cut(s) 267
ApoI RAATTY 1 cut(s) 569
AsuHPI GGTGA 2 cut(s) 161, 521
AxyI CCTNAGG 1 cut(s) 447
Bbv12I GWGCWC 2 cut(s) 166, 178
BccI CCATC 2 cut(s) 15, 284
BceAI ACGGC 2 cut(s) 426, 628
BcgI CGANNNNNNTGC 2 cut(s) 874, 908
BciT130I CCWGG 1 cut(s) 429
BclI TGATCA 1 cut(s) 1084
BcoDI GTCTC 5 cut(s) 150, 213, 265, 273, 336
BfaI CTAG 1 cut(s) 539
BfrI CTTAAG 1 cut(s) 364
BlpI GCTNAGC 1 cut(s) 639
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 1 cut(s) 429
BmgBI CACGTC 1 cut(s) 904
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 3 cut(s) 303, 478, 694
BplI GAGNNNNNCTC 2 cut(s) 449, 481
Bpu10I CCTNAGC 2 cut(s) 351, 1068
Bpu1102I GCTNAGC 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 68
BsaAI YACGTR 2 cut(s) 119, 143
BsaBI GATNNNNATC 2 cut(s) 198, 1083
BsaI GGTCTC 2 cut(s) 150, 273
BsaWI WCCGGW 1 cut(s) 267
Bse21I CCTNAGG 1 cut(s) 447
Bse8I GATNNNNATC 2 cut(s) 198, 1083
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 1 cut(s) 429
BseGI GGATG 5 cut(s) 26, 264, 490, 709, 1063
BseJI GATNNNNATC 2 cut(s) 198, 1083
BseMII CTCAG 6 cut(s) 146, 342, 461, 489, 653, 657
Bsh1236I CGCG 1 cut(s) 896
BsiHKAI GWGCWC 2 cut(s) 166, 178
BsiSI CCGG 2 cut(s) 268, 1007
BsmAI GTCTC 5 cut(s) 150, 213, 265, 273, 336
BsmBI CGTCTC 1 cut(s) 265
Bso31I GGTCTC 2 cut(s) 150, 273
Bsp1286I GDGCHC 2 cut(s) 166, 178
Bsp13I TCCGGA 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 984
Bsp143I GATC 4 cut(s) 790, 866, 1009, 1084
Bsp1720I GCTNAGC 1 cut(s) 639
Bsp68I TCGCGA 1 cut(s) 896
BspACI CCGC 1 cut(s) 707
BspCNI CTCAG 6 cut(s) 145, 343, 460, 488, 652, 658
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 1 cut(s) 896
BspHI TCATGA 2 cut(s) 621, 1151
BspPI GGATC 1 cut(s) 1017
BspQI GCTCTTC 1 cut(s) 171
BspTI CTTAAG 1 cut(s) 364
BspTNI GGTCTC 2 cut(s) 150, 273
BsrGI TGTACA 1 cut(s) 984
BssMI GATC 4 cut(s) 790, 866, 1009, 1084
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 2 cut(s) 161, 650
Bst6I CTCTTC 4 cut(s) 171, 191, 516, 603
BstAFI CTTAAG 1 cut(s) 364
BstAUI TGTACA 1 cut(s) 984
BstBAI YACGTR 2 cut(s) 119, 143
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 8 cut(s) 132, 351, 447, 475, 639, 666, 1068, 1112
BstF5I GGATG 5 cut(s) 26, 264, 490, 709, 1063
BstFNI CGCG 1 cut(s) 896
BstKTI GATC 4 cut(s) 793, 869, 1012, 1087
BstMAI GTCTC 5 cut(s) 150, 213, 265, 273, 336
BstMBI GATC 4 cut(s) 790, 866, 1009, 1084
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 1061
BstSCI CCNGG 1 cut(s) 427
BstSNI TACGTA 2 cut(s) 119, 143
BstUI CGCG 1 cut(s) 896
Bsu36I CCTNAGG 1 cut(s) 447
BtrI CACGTC 1 cut(s) 904
BtsCI GGATG 5 cut(s) 26, 264, 490, 709, 1063
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 2 cut(s) 71, 1104
BtuMI TCGCGA 1 cut(s) 896
Cac8I GCNNGC 1 cut(s) 383
CciI TCATGA 2 cut(s) 621, 1151
Csp6I GTAC 6 cut(s) 371, 584, 646, 819, 919, 985
CviAII CATG 3 cut(s) 622, 1058, 1152
CviQI GTAC 6 cut(s) 371, 584, 646, 819, 919, 985
DdeI CTNAG 8 cut(s) 132, 351, 447, 475, 639, 666, 1068, 1112
DpnI GATC 4 cut(s) 792, 868, 1011, 1086
DpnII GATC 4 cut(s) 790, 866, 1009, 1084
DraIII CACNNNGTG 1 cut(s) 403
Eam1104I CTCTTC 4 cut(s) 171, 191, 516, 603
EarI CTCTTC 4 cut(s) 171, 191, 516, 603
Eco105I TACGTA 2 cut(s) 119, 143
Eco31I GGTCTC 2 cut(s) 150, 273
Eco57I CTGAAG 2 cut(s) 47, 843
Eco81I CCTNAGG 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 427
EcoT22I ATGCAT 1 cut(s) 1059
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 3 cut(s) 625, 1061, 1155
FalI AAGNNNNNCTT 2 cut(s) 856, 888
FatI CATG 3 cut(s) 621, 1057, 1151
FauNDI CATATG 1 cut(s) 1143
FbaI TGATCA 1 cut(s) 1084
FokI GGATG 5 cut(s) 33, 251, 497, 716, 1050
FspBI CTAG 1 cut(s) 539
HapII CCGG 2 cut(s) 268, 1007
Hin1II CATG 3 cut(s) 625, 1061, 1155
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 1070
HinfI GANTC 2 cut(s) 199, 394
HpaII CCGG 2 cut(s) 268, 1007
HphI GGTGA 2 cut(s) 161, 521
Hpy166II GTNNAC 6 cut(s) 152, 406, 442, 693, 985, 996
Hpy188I TCNGA 6 cut(s) 20, 238, 484, 795, 806, 1089
Hpy188III TCNNGA 9 cut(s) 85, 268, 287, 539, 566, 622, 895, 1076, 1152
Hpy8I GTNNAC 6 cut(s) 152, 406, 442, 693, 985, 996
HpyAV CCTTC 5 cut(s) 257, 293, 542, 592, 1022
HpyCH4III ACNGT 2 cut(s) 161, 650
HpyCH4IV ACGT 5 cut(s) 118, 142, 274, 903, 990
HpyCH4V TGCA 2 cut(s) 765, 1057
HpyF3I CTNAG 8 cut(s) 132, 351, 447, 475, 639, 666, 1068, 1112
HpySE526I ACGT 5 cut(s) 118, 142, 274, 903, 990
Hsp92II CATG 3 cut(s) 625, 1061, 1155
Kpn2I TCCGGA 1 cut(s) 267
Ksp22I TGATCA 1 cut(s) 1084
Kzo9I GATC 4 cut(s) 790, 866, 1009, 1084
LguI GCTCTTC 1 cut(s) 171
LpnPI CCDG 8 cut(s) 281, 300, 363, 414, 434, 441, 566, 1020
LweI GCATC 3 cut(s) 303, 478, 694
MaeI CTAG 1 cut(s) 539
MaeII ACGT 5 cut(s) 118, 142, 274, 903, 990
MaeIII GTNAC 3 cut(s) 119, 135, 971
MalI GATC 4 cut(s) 792, 868, 1011, 1086
MboI GATC 4 cut(s) 790, 866, 1009, 1084
MhlI GDGCHC 2 cut(s) 166, 178
MluCI AATT 3 cut(s) 433, 569, 631
MmeI TCCRAC 2 cut(s) 261, 893
Mph1103I ATGCAT 1 cut(s) 1059
MroI TCCGGA 1 cut(s) 267
MseI TTAA 3 cut(s) 365, 417, 851
MspCI CTTAAG 1 cut(s) 364
MspI CCGG 2 cut(s) 268, 1007
MspR9I CCNGG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 429
MvnI CGCG 1 cut(s) 896
NdeI CATATG 1 cut(s) 1143
NdeII GATC 4 cut(s) 790, 866, 1009, 1084
NlaIII CATG 3 cut(s) 625, 1061, 1155
NruI TCGCGA 1 cut(s) 896
NsiI ATGCAT 1 cut(s) 1059
NspI RCATGY 1 cut(s) 1061
PagI TCATGA 2 cut(s) 621, 1151
PciSI GCTCTTC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PfeI GAWTC 2 cut(s) 199, 394
Ppu21I YACGTR 2 cut(s) 119, 143
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PsrI GAACNNNNNNTAC 2 cut(s) 80, 112
RruI TCGCGA 1 cut(s) 896
RsaI GTAC 6 cut(s) 372, 585, 647, 820, 920, 986
RsaNI GTAC 6 cut(s) 371, 584, 646, 819, 919, 985
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 3 cut(s) 365, 417, 851
Sau3AI GATC 4 cut(s) 790, 866, 1009, 1084
ScaI AGTACT 1 cut(s) 820
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 2 cut(s) 166, 178
SfaNI GCATC 3 cut(s) 303, 478, 694
SmlI CTYRAG 2 cut(s) 83, 364
SmoI CTYRAG 2 cut(s) 83, 364
SnaBI TACGTA 2 cut(s) 119, 143
Sse9I AATT 3 cut(s) 433, 569, 631
SsiI CCGC 1 cut(s) 707
SspMI CTAG 1 cut(s) 539
StyD4I CCNGG 1 cut(s) 427
TaaI ACNGT 2 cut(s) 161, 650
TaiI ACGT 5 cut(s) 121, 145, 277, 906, 993
TaqI TCGA 1 cut(s) 555
TasI AATT 3 cut(s) 433, 569, 631
TatI WGTACW 2 cut(s) 818, 984
TfiI GAWTC 2 cut(s) 199, 394
Tru1I TTAA 3 cut(s) 365, 417, 851
Tru9I TTAA 3 cut(s) 365, 417, 851
TscAI CASTG 2 cut(s) 71, 1111
TspDTI ATGAA 6 cut(s) 158, 315, 642, 864, 1052, 1140
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 2 cut(s) 71, 1111
Vha464I CTTAAG 1 cut(s) 364
XapI RAATTY 1 cut(s) 569
XbaI TCTAGA 1 cut(s) 538
XceI RCATGY 1 cut(s) 1061
XspI CTAG 1 cut(s) 539
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.