Rh2BG577100

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013512
Physical Location & Seq
Forward (+)
79358277 .. 79359395
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG577100.1

Sequence Viewer

Length: 1119 bp
ATGATATACAATTCAAGTCCTTTGTTCCATGCACCTCAGGTAAAGGAAATTAGTGGACATCAAGCACTGGATTCAAATGTTCGGACTTACTACGAGAGATCTGAAATGCAAATTTGCTCTGTCGAAGGGCTTGAAACAGAAGATGTTCATGATTCCAGTATATCTACTACTACTTCTTCAGTAGCTAAGCTGGCCCCGACTGTATCAGACTCTCCGGGGCATAATGAAATACAGGAAGAACAGGACTTTGTTGAAGTGGATTCTCCAAGGGGTGGAGAAATAGTGGTGTACAATGATGACATTGAAGAGGTTCAGTCTTGCAGAACAAGAGATGGAGATGACTGCCAGGACCAAAGAGCTTTCAAGTTCAAAGCTAGTGTTCCGAAAAAGGAGAATCTTGAAAACAGCTTTGATCGTGGTCATATGAACACATGGAGTGTGAATTCAGGGTCAAGAGTCTCTACGAGAAGTTTTGGGGGAAATGGGAAAAGACCTAGGAAGAGATCAGTGTCTCAGGAAAATCAGTTTTCGAAGGATAGTGAATTTTCAAGTTACTATGCTAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTGGTAAATCCATGTTATGTTTGCAATGGGAATGAAAATGTCGAGTTTGAAGTTCCAACATTGAAGCAAAATAGAGGGATTTCAACTAGGGTGGGATTTGCTAATCCTGGTCATGAACATAAAAGTTTGGTGTACAATGTTTTCACTTACCCTGATCACCAGCCAAAACCGCAAAGCAAAGTGTTCAAAGGAAACATAGAGGAATCTGGTTTTCAATGCAAGCTTCCCTCTTATACTTCGTCTTCATCATCATCATCTTCTCCAACTGTGACTAGTTCTTGGACGAGAAAAGAAATGGTACCTCCTTACTTCAGAAATGTGACAATGCCGCCGGAAAGAGTCAACGACAAGCGGAAAGAAGAGTTTCAACGATCCATTTCGGACTGTTTTCAGTATCCTACTCATGTTCATCCAAAGCTGCCAGATTATGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAAACAAAGCCTCCTCTCAGCAATCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

372

Amino Acids

42.12

Weight (kDa)

7.15

Isoelectric Point (pI)

54.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 910
AccB1I GGYRCC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 272
AciI CCGC 3 cut(s) 782, 941, 964
AclWI GGATC 1 cut(s) 978
AcsI RAATTY 4 cut(s) 111, 442, 542, 1058
AcuI CTGAAG 2 cut(s) 162, 907
AfaI GTAC 3 cut(s) 290, 746, 912
AfiI CCNNNNNNNGG 1 cut(s) 272
AhlI ACTAGT 1 cut(s) 884
AjnI CCWGG 3 cut(s) 345, 612, 718
AluBI AGCT 9 cut(s) 185, 190, 359, 374, 408, 835, 1030, 1055, 1114
AluI AGCT 9 cut(s) 185, 190, 359, 374, 408, 835, 1030, 1055, 1114
Alw26I GTCTC 2 cut(s) 463, 516
AlwI GGATC 1 cut(s) 978
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 3 cut(s) 609, 1030, 1052
ApoI RAATTY 4 cut(s) 111, 442, 542, 1058
Asp700I GAANNNNTTC 3 cut(s) 144, 309, 975
Asp718I GGTACC 1 cut(s) 910
AspA2I CCTAGG 1 cut(s) 494
AspS9I GGNCC 3 cut(s) 193, 349, 584
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 761
AsuII TTCGAA 1 cut(s) 530
AvaII GGWCC 2 cut(s) 349, 584
AvrII CCTAGG 1 cut(s) 494
AxyI CCTNAGG 1 cut(s) 36
BanI GGYRCC 1 cut(s) 910
BbsI GAAGAC 1 cut(s) 846
BbvI GCAGC 3 cut(s) 621, 1017, 1064
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 3 cut(s) 347, 614, 720
BciVI GTATCC 1 cut(s) 1017
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 2 cut(s) 463, 516
BcuI ACTAGT 1 cut(s) 884
BfaI CTAG 5 cut(s) 375, 495, 561, 699, 885
BfuI GTATCC 1 cut(s) 1017
BglII AGATCT 1 cut(s) 98
BisI GCNGC 4 cut(s) 610, 941, 1031, 1053
BlnI CCTAGG 1 cut(s) 494
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 4 cut(s) 611, 942, 1032, 1054
Bme1390I CCNGG 4 cut(s) 216, 347, 614, 720
Bme18I GGWCC 2 cut(s) 349, 584
BmgT120I GGNCC 3 cut(s) 193, 349, 584
BmiI GGNNCC 3 cut(s) 195, 585, 912
BmrFI CCNGG 4 cut(s) 216, 347, 614, 720
BmsI GCATC 1 cut(s) 577
BpiI GAAGAC 1 cut(s) 846
Bpu1102I GCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 215, 266, 494
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 1081, 1111
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 72, 156
Bse21I CCTNAGG 1 cut(s) 36
Bse3DI GCAATG 1 cut(s) 643
BseBI CCWGG 3 cut(s) 347, 614, 720
BseDI CCNNGG 3 cut(s) 215, 266, 494
BseGI GGATG 1 cut(s) 1021
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 3 cut(s) 50, 527, 1117
BseNI ACTGG 2 cut(s) 72, 156
BseRI GAGGAG 1 cut(s) 1089
BseXI GCAGC 3 cut(s) 621, 1017, 1064
BsgI GTGCAG 1 cut(s) 628
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 910
BsiSI CCGG 2 cut(s) 215, 944
BslFI GGGAC 1 cut(s) 597
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 2 cut(s) 463, 516
BsmFI GGGAC 1 cut(s) 597
BsnI GGCC 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 530
Bsp1407I TGTACA 2 cut(s) 288, 744
Bsp143I GATC 5 cut(s) 98, 412, 503, 766, 983
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 3 cut(s) 782, 941, 964
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 3 cut(s) 49, 526, 1116
BspHI TCATGA 2 cut(s) 148, 724
BspLI GGNNCC 3 cut(s) 195, 585, 912
BspPI GGATC 1 cut(s) 978
BspT104I TTCGAA 1 cut(s) 530
BspT107I GGYRCC 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 643
BsrGI TGTACA 2 cut(s) 288, 744
BsrI ACTGG 2 cut(s) 72, 156
BssECI CCNNGG 3 cut(s) 215, 266, 494
BssMI GATC 5 cut(s) 98, 412, 503, 766, 983
BssT1I CCWWGG 2 cut(s) 266, 494
Bst2UI CCWGG 3 cut(s) 347, 614, 720
Bst4CI ACNGT 3 cut(s) 202, 880, 998
Bst6I CTCTTC 3 cut(s) 300, 494, 966
BstAUI TGTACA 2 cut(s) 288, 744
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 192, 833
BstDEI CTNAG 4 cut(s) 36, 186, 513, 1103
BstF5I GGATG 1 cut(s) 1021
BstKTI GATC 5 cut(s) 101, 415, 506, 769, 986
BstMAI GTCTC 2 cut(s) 463, 516
BstMBI GATC 5 cut(s) 98, 412, 503, 766, 983
BstMWI GCNNNNNNNGC 4 cut(s) 191, 609, 781, 1093
BstNI CCWGG 3 cut(s) 347, 614, 720
BstSCI CCNGG 4 cut(s) 214, 345, 612, 718
BstV1I GCAGC 3 cut(s) 621, 1017, 1064
BstV2I GAAGAC 1 cut(s) 846
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 36
BsuI GTATCC 1 cut(s) 1017
BsuRI GGCC 1 cut(s) 194
BtsCI GGATG 1 cut(s) 1021
BtsIMutI CAGTG 2 cut(s) 65, 513
Cac8I GCNNGC 2 cut(s) 192, 833
CciI TCATGA 2 cut(s) 148, 724
Cfr13I GGNCC 3 cut(s) 193, 349, 584
Csp6I GTAC 3 cut(s) 289, 745, 911
CspCI CAANNNNNGTGG 2 cut(s) 684, 719
CviAII CATG 8 cut(s) 29, 149, 432, 600, 624, 725, 1016, 1063
CviQI GTAC 3 cut(s) 289, 745, 911
DdeI CTNAG 4 cut(s) 36, 186, 513, 1103
DpnI GATC 5 cut(s) 100, 414, 505, 768, 985
DpnII GATC 5 cut(s) 98, 412, 503, 766, 983
Eam1104I CTCTTC 3 cut(s) 300, 494, 966
EarI CTCTTC 3 cut(s) 300, 494, 966
Eco130I CCWWGG 2 cut(s) 266, 494
Eco47I GGWCC 2 cut(s) 349, 584
Eco57I CTGAAG 2 cut(s) 162, 907
Eco81I CCTNAGG 1 cut(s) 36
EcoO109I RGGNCCY 1 cut(s) 584
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 3 cut(s) 345, 612, 718
EcoT14I CCWWGG 2 cut(s) 266, 494
ErhI CCWWGG 2 cut(s) 266, 494
FaeI CATG 8 cut(s) 32, 152, 435, 603, 627, 728, 1019, 1066
FaqI GGGAC 1 cut(s) 597
FatI CATG 8 cut(s) 28, 148, 431, 599, 623, 724, 1015, 1062
FauNDI CATATG 1 cut(s) 423
FbaI TGATCA 1 cut(s) 766
Fnu4HI GCNGC 4 cut(s) 610, 941, 1031, 1053
FokI GGATG 1 cut(s) 1008
Fsp4HI GCNGC 4 cut(s) 610, 941, 1031, 1053
FspBI CTAG 5 cut(s) 375, 495, 561, 699, 885
GluI GCNGC 4 cut(s) 610, 941, 1031, 1053
HaeIII GGCC 1 cut(s) 194
HapII CCGG 2 cut(s) 215, 944
Hin1II CATG 8 cut(s) 32, 152, 435, 603, 627, 728, 1019, 1066
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 8 cut(s) 71, 152, 209, 260, 394, 456, 815, 951
HpaII CCGG 2 cut(s) 215, 944
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 4 cut(s) 56, 289, 745, 955
Hpy188I TCNGA 7 cut(s) 84, 103, 208, 384, 573, 926, 994
Hpy188III TCNNGA 6 cut(s) 149, 398, 453, 515, 725, 1072
Hpy8I GTNNAC 4 cut(s) 56, 289, 745, 955
HpyAV CCTTC 2 cut(s) 119, 526
HpyCH4III ACNGT 3 cut(s) 202, 880, 998
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 9 cut(s) 32, 109, 321, 603, 609, 636, 831, 1052, 1087
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 609, 781, 1093
HpyF3I CTNAG 4 cut(s) 36, 186, 513, 1103
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 8 cut(s) 32, 152, 435, 603, 627, 728, 1019, 1066
KpnI GGTACC 1 cut(s) 914
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 5 cut(s) 98, 412, 503, 766, 983
Lsp1109I GCAGC 3 cut(s) 621, 1017, 1064
LweI GCATC 1 cut(s) 577
MaeI CTAG 5 cut(s) 375, 495, 561, 699, 885
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 3 cut(s) 551, 880, 931
MalI GATC 5 cut(s) 100, 414, 505, 768, 985
MboI GATC 5 cut(s) 98, 412, 503, 766, 983
MboII GAAGA 9 cut(s) 152, 168, 248, 317, 511, 586, 846, 861, 983
MflI RGATCY 1 cut(s) 98
MluCI AATT 7 cut(s) 10, 48, 111, 442, 542, 578, 1058
MlyI GAGTC 3 cut(s) 203, 465, 960
MmeI TCCRAC 2 cut(s) 692, 899
MnlI CCTC 8 cut(s) 45, 301, 680, 805, 850, 924, 1107, 1110
MroXI GAANNNNTTC 3 cut(s) 144, 309, 975
MslI CAYNNNNRTG 1 cut(s) 604
MspI CCGG 2 cut(s) 215, 944
MspR9I CCNGG 4 cut(s) 216, 347, 614, 720
MvaI CCWGG 3 cut(s) 347, 614, 720
MwoI GCNNNNNNNGC 4 cut(s) 191, 609, 781, 1093
NciI CCSGG 1 cut(s) 216
NdeI CATATG 1 cut(s) 423
NdeII GATC 5 cut(s) 98, 412, 503, 766, 983
NlaIII CATG 8 cut(s) 32, 152, 435, 603, 627, 728, 1019, 1066
NlaIV GGNNCC 3 cut(s) 195, 585, 912
NmuCI GTSAC 2 cut(s) 880, 931
NspV TTCGAA 1 cut(s) 530
PagI TCATGA 2 cut(s) 148, 724
PdmI GAANNNNTTC 3 cut(s) 144, 309, 975
PfeI GAWTC 5 cut(s) 71, 152, 260, 394, 815
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 4 cut(s) 611, 942, 1032, 1054
PleI GAGTC 3 cut(s) 203, 464, 959
PpsI GAGTC 3 cut(s) 203, 464, 959
PpuMI RGGWCCY 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 584
Psp6I CCWGG 3 cut(s) 345, 612, 718
PspGI CCWGG 3 cut(s) 345, 612, 718
PspN4I GGNNCC 3 cut(s) 195, 585, 912
PspPI GGNCC 3 cut(s) 193, 349, 584
PspPPI RGGWCCY 1 cut(s) 584
PsuI RGATCY 1 cut(s) 98
RsaI GTAC 3 cut(s) 290, 746, 912
RsaNI GTAC 3 cut(s) 289, 745, 911
RseI CAYNNNNRTG 1 cut(s) 604
SatI GCNGC 4 cut(s) 610, 941, 1031, 1053
Sau3AI GATC 5 cut(s) 98, 412, 503, 766, 983
Sau96I GGNCC 3 cut(s) 193, 349, 584
SchI GAGTC 3 cut(s) 203, 465, 960
ScrFI CCNGG 4 cut(s) 216, 347, 614, 720
SfaNI GCATC 1 cut(s) 577
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 2 cut(s) 349, 584
SmiMI CAYNNNNRTG 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
SpeI ACTAGT 1 cut(s) 884
Sse9I AATT 7 cut(s) 10, 48, 111, 442, 542, 578, 1058
SsiI CCGC 3 cut(s) 782, 941, 964
SspMI CTAG 5 cut(s) 375, 495, 561, 699, 885
StyD4I CCNGG 4 cut(s) 214, 345, 612, 718
StyI CCWWGG 2 cut(s) 266, 494
TaaI ACNGT 3 cut(s) 202, 880, 998
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 3 cut(s) 123, 530, 654
TasI AATT 7 cut(s) 10, 48, 111, 442, 542, 578, 1058
TatI WGTACW 2 cut(s) 288, 744
TauI GCSGC 1 cut(s) 943
TfiI GAWTC 5 cut(s) 71, 152, 260, 394, 815
TscAI CASTG 2 cut(s) 72, 513
TseFI GTSAC 2 cut(s) 880, 931
TseI GCWGC 3 cut(s) 609, 1030, 1052
Tsp45I GTSAC 2 cut(s) 880, 931
TspDTI ATGAA 8 cut(s) 137, 240, 440, 660, 741, 846, 1010, 1051
TspRI CASTG 2 cut(s) 72, 513
Van91I CCANNNNNTGG 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 349, 584
XapI RAATTY 4 cut(s) 111, 442, 542, 1058
XmaJI CCTAGG 1 cut(s) 494
XmnI GAANNNNTTC 3 cut(s) 144, 309, 975
XspI CTAG 5 cut(s) 375, 495, 561, 699, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.