Rh2AG564400

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013512
Physical Location & Seq
Forward (+)
79472399 .. 79475527
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG564400.1

Sequence Viewer

Length: 1173 bp
ATGAAGCACAGATTGGTCAATGAAATAGCAAGGAATTACTGCATCAAACCAGAGGTTTTAGCCATCGAATACTATTCCGATTGGCAACAGAAGATAAAGGAAATTAGTGGACATCAAGCACTGGATTCAAATGTTCGGACTTACTACGAGAGATCTGAAATGCAAATTTGCTCTGTCGAAGGGCTTGAAACAGAAGATGTTCATGATTCCAGTATATCTACTACTACTTCTTTAGTAGCTAAGCTGACCCCGACTGTATCAGACTCTCCGGTGTATAATGAAATACAGGAAGAACGGGACTTTGCTGAAGTGGATTCTCCAAGGGGTGGAGAAATAGTGGTGTACAATGATGACATTGAAGAGGTTCAGTCTTGCACAACTAGAGATGGAGATGACTGCCAGGACCAAAGAGCTTTCAAGTTCAAAGCTAGTGTTCCGAAAAAGGAGAATCTTGAAAACAGCTTTGATCGTGGTCATATGAACACATGGAGTGTGAATTCAGGGTCAAGAGTCTCTACGAGATGTTTTGGGGGAAATGGGAAAAGACCAAGGAAGAGATCAGTGTCTCAGGAAAATCAGTTTTCGAAGGATAGTGAATTTCCAAGTTACTATGCTAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTAGTAAATCCATGTTATGTTTGCAATGGGAATGAAAATGTCGAATTTGAAGTTCCAACATTGAAGCAAAATAGAGGGATTTCAACTAGGGTGGGATTTGCTAATCCTGGTCATGAACATAAAAGGTTGGTGTACAATGTGTTCACTTACCCTGATCACCAGCCAAAACCGCAAAGCAAAGTGTTCAAAGGAAACATAGAGGAATCTGGTTTTCAATGCAAGCTTCCCTCTTATACTTCGTCTTCATCATCATCATCTTCTCCAACTGTGACTAGTTCTTGGACGAGAAAAGAAATGGTACCTCCTTACTTCAGAAATGTGACAATGCCGCCGGAAAGAGTCAACGACAAGCGGAAAGAAGAGTTTCAACGATCCATTTCAGACTGTTTTCAGTTTCCTACTCATGTTCATCCAAAGCTGCCAGATTATGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAAACTAAGCCTCCTCTCAGCAATCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

390

Amino Acids

44.62

Weight (kDa)

7.93

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 964
AccB1I GGYRCC 1 cut(s) 964
AccB7I CCANNNNNTGG 1 cut(s) 326
AciI CCGC 3 cut(s) 836, 995, 1018
AclWI GGATC 1 cut(s) 1032
AcsI RAATTY 5 cut(s) 165, 496, 596, 710, 1112
AcuI CTGAAG 2 cut(s) 327, 961
AfaI GTAC 3 cut(s) 344, 800, 966
AfiI CCNNNNNNNGG 1 cut(s) 326
AhlI ACTAGT 1 cut(s) 938
AjnI CCWGG 2 cut(s) 399, 772
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 9 cut(s) 239, 244, 413, 428, 462, 889, 1084, 1109, 1168
AluI AGCT 9 cut(s) 239, 244, 413, 428, 462, 889, 1084, 1109, 1168
Alw26I GTCTC 2 cut(s) 517, 570
AlwI GGATC 1 cut(s) 1032
ApeKI GCWGC 3 cut(s) 663, 1084, 1106
ApoI RAATTY 5 cut(s) 165, 496, 596, 710, 1112
Asp700I GAANNNNTTC 3 cut(s) 198, 363, 1029
Asp718I GGTACC 1 cut(s) 964
AspS9I GGNCC 2 cut(s) 403, 638
AsuHPI GGTGA 1 cut(s) 815
AsuII TTCGAA 1 cut(s) 584
AvaII GGWCC 2 cut(s) 403, 638
BanI GGYRCC 1 cut(s) 964
BbsI GAAGAC 1 cut(s) 900
BbvI GCAGC 3 cut(s) 675, 1071, 1118
BccI CCATC 2 cut(s) 71, 380
BciT130I CCWGG 2 cut(s) 401, 774
BclI TGATCA 1 cut(s) 820
BcoDI GTCTC 2 cut(s) 517, 570
BcuI ACTAGT 1 cut(s) 938
BfaI CTAG 6 cut(s) 381, 429, 615, 668, 753, 939
BglII AGATCT 1 cut(s) 152
BisI GCNGC 4 cut(s) 664, 995, 1085, 1107
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 4 cut(s) 665, 996, 1086, 1108
Bme1390I CCNGG 2 cut(s) 401, 774
Bme18I GGWCC 2 cut(s) 403, 638
BmgT120I GGNCC 2 cut(s) 403, 638
BmiI GGNNCC 2 cut(s) 639, 966
BmrFI CCNGG 2 cut(s) 401, 774
BmsI GCATC 2 cut(s) 51, 631
BpiI GAAGAC 1 cut(s) 900
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 584
BpuEI CTTGAG 1 cut(s) 1109
BsaJI CCNNGG 2 cut(s) 320, 548
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 4 cut(s) 321, 351, 1135, 1165
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 126, 210
Bse3DI GCAATG 1 cut(s) 697
BseBI CCWGG 2 cut(s) 401, 774
BseDI CCNNGG 2 cut(s) 320, 548
BseGI GGATG 1 cut(s) 1075
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMI GCAATG 1 cut(s) 697
BseMII CTCAG 2 cut(s) 581, 1171
BseNI ACTGG 2 cut(s) 126, 210
BseRI GAGGAG 1 cut(s) 1143
BseXI GCAGC 3 cut(s) 675, 1071, 1118
BsgI GTGCAG 1 cut(s) 682
BshNI GGYRCC 1 cut(s) 964
BsiSI CCGG 2 cut(s) 269, 998
BslFI GGGAC 2 cut(s) 311, 651
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 2 cut(s) 517, 570
BsmFI GGGAC 2 cut(s) 311, 651
Bsp119I TTCGAA 1 cut(s) 584
Bsp1407I TGTACA 2 cut(s) 342, 798
Bsp143I GATC 5 cut(s) 152, 466, 557, 820, 1037
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 3 cut(s) 836, 995, 1018
BspCNI CTCAG 2 cut(s) 580, 1170
BspHI TCATGA 2 cut(s) 202, 778
BspLI GGNNCC 2 cut(s) 639, 966
BspPI GGATC 1 cut(s) 1032
BspT104I TTCGAA 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 964
BsrDI GCAATG 1 cut(s) 697
BsrGI TGTACA 2 cut(s) 342, 798
BsrI ACTGG 2 cut(s) 126, 210
BssECI CCNNGG 2 cut(s) 320, 548
BssMI GATC 5 cut(s) 152, 466, 557, 820, 1037
BssT1I CCWWGG 2 cut(s) 320, 548
Bst2UI CCWGG 2 cut(s) 401, 774
Bst4CI ACNGT 3 cut(s) 256, 934, 1052
Bst6I CTCTTC 3 cut(s) 354, 548, 1020
BstAUI TGTACA 2 cut(s) 342, 798
BstBI TTCGAA 1 cut(s) 584
BstC8I GCNNGC 1 cut(s) 887
BstDEI CTNAG 4 cut(s) 240, 567, 1146, 1157
BstF5I GGATG 1 cut(s) 1075
BstKTI GATC 5 cut(s) 155, 469, 560, 823, 1040
BstMAI GTCTC 2 cut(s) 517, 570
BstMBI GATC 5 cut(s) 152, 466, 557, 820, 1037
BstMWI GCNNNNNNNGC 3 cut(s) 663, 835, 1147
BstNI CCWGG 2 cut(s) 401, 774
BstSCI CCNGG 2 cut(s) 399, 772
BstV1I GCAGC 3 cut(s) 675, 1071, 1118
BstV2I GAAGAC 1 cut(s) 900
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BtsCI GGATG 1 cut(s) 1075
BtsIMutI CAGTG 2 cut(s) 119, 567
Cac8I GCNNGC 1 cut(s) 887
CciI TCATGA 2 cut(s) 202, 778
Cfr13I GGNCC 2 cut(s) 403, 638
Csp6I GTAC 3 cut(s) 343, 799, 965
CspCI CAANNNNNGTGG 2 cut(s) 738, 773
CviAII CATG 7 cut(s) 203, 486, 654, 678, 779, 1070, 1117
CviQI GTAC 3 cut(s) 343, 799, 965
DdeI CTNAG 4 cut(s) 240, 567, 1146, 1157
DpnI GATC 5 cut(s) 154, 468, 559, 822, 1039
DpnII GATC 5 cut(s) 152, 466, 557, 820, 1037
Eam1104I CTCTTC 3 cut(s) 354, 548, 1020
EarI CTCTTC 3 cut(s) 354, 548, 1020
Eco130I CCWWGG 2 cut(s) 320, 548
Eco47I GGWCC 2 cut(s) 403, 638
Eco57I CTGAAG 2 cut(s) 327, 961
EcoO109I RGGNCCY 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 496
EcoRII CCWGG 2 cut(s) 399, 772
EcoT14I CCWWGG 2 cut(s) 320, 548
ErhI CCWWGG 2 cut(s) 320, 548
FaeI CATG 7 cut(s) 206, 489, 657, 681, 782, 1073, 1120
FaqI GGGAC 2 cut(s) 311, 651
FatI CATG 7 cut(s) 202, 485, 653, 677, 778, 1069, 1116
FauNDI CATATG 1 cut(s) 477
FbaI TGATCA 1 cut(s) 820
Fnu4HI GCNGC 4 cut(s) 664, 995, 1085, 1107
FokI GGATG 1 cut(s) 1062
Fsp4HI GCNGC 4 cut(s) 664, 995, 1085, 1107
FspBI CTAG 6 cut(s) 381, 429, 615, 668, 753, 939
GluI GCNGC 4 cut(s) 664, 995, 1085, 1107
HapII CCGG 2 cut(s) 269, 998
Hin1II CATG 7 cut(s) 206, 489, 657, 681, 782, 1073, 1120
HincII GTYRAC 1 cut(s) 1009
HindII GTYRAC 1 cut(s) 1009
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 8 cut(s) 125, 206, 263, 314, 448, 510, 869, 1005
HpaII CCGG 2 cut(s) 269, 998
HphI GGTGA 1 cut(s) 815
Hpy166II GTNNAC 5 cut(s) 110, 343, 799, 810, 1009
Hpy188I TCNGA 8 cut(s) 79, 138, 157, 262, 438, 627, 980, 1048
Hpy188III TCNNGA 6 cut(s) 203, 452, 507, 569, 779, 1126
Hpy8I GTNNAC 5 cut(s) 110, 343, 799, 810, 1009
HpyAV CCTTC 2 cut(s) 173, 580
HpyCH4III ACNGT 3 cut(s) 256, 934, 1052
HpyCH4IV ACGT 1 cut(s) 1135
HpyCH4V TGCA 9 cut(s) 42, 163, 375, 657, 663, 690, 885, 1106, 1141
HpyF10VI GCNNNNNNNGC 3 cut(s) 663, 835, 1147
HpyF3I CTNAG 4 cut(s) 240, 567, 1146, 1157
HpySE526I ACGT 1 cut(s) 1135
Hsp92II CATG 7 cut(s) 206, 489, 657, 681, 782, 1073, 1120
KpnI GGTACC 1 cut(s) 968
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 5 cut(s) 152, 466, 557, 820, 1037
Lsp1109I GCAGC 3 cut(s) 675, 1071, 1118
LweI GCATC 2 cut(s) 51, 631
MaeI CTAG 6 cut(s) 381, 429, 615, 668, 753, 939
MaeII ACGT 1 cut(s) 1135
MaeIII GTNAC 3 cut(s) 605, 934, 985
MalI GATC 5 cut(s) 154, 468, 559, 822, 1039
MboI GATC 5 cut(s) 152, 466, 557, 820, 1037
MboII GAAGA 9 cut(s) 103, 206, 302, 371, 565, 640, 900, 915, 1037
MflI RGATCY 1 cut(s) 152
MluCI AATT 8 cut(s) 34, 102, 165, 496, 596, 632, 710, 1112
MlyI GAGTC 3 cut(s) 257, 519, 1014
MmeI TCCRAC 2 cut(s) 746, 953
MnlI CCTC 8 cut(s) 46, 355, 734, 859, 904, 978, 1161, 1164
MroXI GAANNNNTTC 3 cut(s) 198, 363, 1029
MslI CAYNNNNRTG 1 cut(s) 658
MspI CCGG 2 cut(s) 269, 998
MspR9I CCNGG 2 cut(s) 401, 774
MvaI CCWGG 2 cut(s) 401, 774
MwoI GCNNNNNNNGC 3 cut(s) 663, 835, 1147
NdeI CATATG 1 cut(s) 477
NdeII GATC 5 cut(s) 152, 466, 557, 820, 1037
NlaIII CATG 7 cut(s) 206, 489, 657, 681, 782, 1073, 1120
NlaIV GGNNCC 2 cut(s) 639, 966
NmuCI GTSAC 2 cut(s) 934, 985
NspV TTCGAA 1 cut(s) 584
PagI TCATGA 2 cut(s) 202, 778
PdmI GAANNNNTTC 3 cut(s) 198, 363, 1029
PfeI GAWTC 5 cut(s) 125, 206, 314, 448, 869
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 4 cut(s) 665, 996, 1086, 1108
PleI GAGTC 3 cut(s) 257, 518, 1013
PpsI GAGTC 3 cut(s) 257, 518, 1013
PpuMI RGGWCCY 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 399, 772
PspGI CCWGG 2 cut(s) 399, 772
PspN4I GGNNCC 2 cut(s) 639, 966
PspPI GGNCC 2 cut(s) 403, 638
PspPPI RGGWCCY 1 cut(s) 638
PsrI GAACNNNNNNTAC 2 cut(s) 791, 823
PsuI RGATCY 1 cut(s) 152
RsaI GTAC 3 cut(s) 344, 800, 966
RsaNI GTAC 3 cut(s) 343, 799, 965
RseI CAYNNNNRTG 1 cut(s) 658
SatI GCNGC 4 cut(s) 664, 995, 1085, 1107
Sau3AI GATC 5 cut(s) 152, 466, 557, 820, 1037
Sau96I GGNCC 2 cut(s) 403, 638
SchI GAGTC 3 cut(s) 257, 519, 1014
ScrFI CCNGG 2 cut(s) 401, 774
SfaNI GCATC 2 cut(s) 51, 631
SfuI TTCGAA 1 cut(s) 584
SinI GGWCC 2 cut(s) 403, 638
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
SpeI ACTAGT 1 cut(s) 938
Sse9I AATT 8 cut(s) 34, 102, 165, 496, 596, 632, 710, 1112
SsiI CCGC 3 cut(s) 836, 995, 1018
SspMI CTAG 6 cut(s) 381, 429, 615, 668, 753, 939
StyD4I CCNGG 2 cut(s) 399, 772
StyI CCWWGG 2 cut(s) 320, 548
TaaI ACNGT 3 cut(s) 256, 934, 1052
TaiI ACGT 1 cut(s) 1138
TaqI TCGA 4 cut(s) 66, 177, 584, 708
TasI AATT 8 cut(s) 34, 102, 165, 496, 596, 632, 710, 1112
TatI WGTACW 2 cut(s) 342, 798
TauI GCSGC 1 cut(s) 997
TfiI GAWTC 5 cut(s) 125, 206, 314, 448, 869
TscAI CASTG 2 cut(s) 126, 567
TseFI GTSAC 2 cut(s) 934, 985
TseI GCWGC 3 cut(s) 663, 1084, 1106
Tsp45I GTSAC 2 cut(s) 934, 985
TspRI CASTG 2 cut(s) 126, 567
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 403, 638
XapI RAATTY 5 cut(s) 165, 496, 596, 710, 1112
XmnI GAANNNNTTC 3 cut(s) 198, 363, 1029
XspI CTAG 6 cut(s) 381, 429, 615, 668, 753, 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.