RchiOBHm_Chr2g0162561

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0013512
Physical Location & Seq
Forward (+)
78264887 .. 78267481
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53081

Sequence Viewer

Length: 1671 bp
ATGTTTGACATATTGTTCGGGTGGAGAAAAGCTTCAAAGTGCAAGCAGCTGATCAAACAGGTGCAGTGCCGGCTCAAGTTGCTGAAGAACAAGAGGGCTACTATTGTGAGGCAATTGCGAGAAGACGCGGCGGAGCTGATCAAGAATGGCTACGAAGGCAAAGCCTTCAATAGGGTATATGGTGTTGAGCAGATAATCAAAGATGAATGCATAGTGGCACTGTATGAACTGTTGGATAATTTCTGTGAATTTATCCTCCTTCACCTTTCATATATCCGAAGACACAAGGACTGTCCAAATGACATAAATGAAGCAGTTTCAAGTCTTATATATGCTTCTGCTAGATGTGGAGATCTCCCTGAGCTTAGAACAATCCGAAAGCTCTTCAGAGAACGTTATGGTCAGAAATATGCAATGACTGCTCTTGAACTATTCCCCGGGAACCTTGTGAACTATCAGGTAATAGAGAAACTGTCCCTAAAGTCAGTAACAGATGATATGAAGCACAGATTGGTCAATGAAATAGCAAGGAATTACTGCATCAAACCAGAGGTTTTAGCCATCGAATACTATTCCGATTGGCAACAGAAGGTAAAGGAAATTAGTGGACATCAAGCACTGGATTCAAATGTTCGGACTTACTACGAGAGATCTGAAATGCAAATTTGCTCTGTCGAAGGGCTTGAAACAGAAGATGTTCATGATTCCAGTATATCTACTACTACTTCTTCAGTAGCTAAGCTGGCCCCGACTGTATCAGACTCTCCGGGGCATAATGAAATACAGGAAGAACAGGACTTTGTTGAAGTGGATTCTCCAAGGGGTGGAGAAATAGTGGTGTACAATGATGACATTGAAGAGGTTCAGTCTTGCAGAACAAGAGATGGAGATGACTGCCAGGACCAAAGAGCTTTCAAGTTCAAAGCTAGTGTTCCGAAAAAGGAGAATCTTGAAAACAGCTTTGATCGTGGTCATATGAACACATGGAGTGTGAATTCAGGGTCAAGAGTCTCTACGAGAAGTTTTGGGGGAAATGGGAAAAGACCTAGGAAGAGATCAGTGTCTCAGGAAAATCAGTTTTCGAAGGATAGTGAATTTTCAAGTTACTATGCTAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTGGTAAATCCATGTTATGTTTGCAATGGGAATGAAAATGTCGAGTTTGAAGTTCCAACATTGAAGCAAAATAGAGGGATTTCAACTAGGGTGGGATTTGCTAATCCTGGTCATGAACATAAAAGTTTGGTGTACAATGTTTTCACTTACCCTGATCACCAGCCAAAACCGCAAAGCAAAGTGTTCAAAGGAAACATAGAGGAATCTGGTTTTCAATGCAAGCTTCCCTCTTATACTTCGTCTTCATCATCATCATCTTCTCCAACTGTGACTAGTTCTTGGACGAGAAAAGAAATGGTACCTCCTTACTTCAGAAATGTGACAATGCCGCCGGAAAGAGTCAACGACAAGCGGAAAGAAGAGTTTCAACGATCCATTTCGGACTGTTTTCAGTATCCTACTCATGTTCATCCAAAGCTGCCAGATTATGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAAACAAAGCCTCCTCTCAGCAATCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

556

Amino Acids

63.82

Weight (kDa)

8.59

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 181 4.8e-49 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1462
AccB1I GGYRCC 1 cut(s) 1462
AccB7I CCANNNNNTGG 1 cut(s) 824
AccII CGCG 1 cut(s) 128
AciI CCGC 5 cut(s) 128, 131, 1334, 1493, 1516
AclI AACGTT 1 cut(s) 394
AclWI GGATC 1 cut(s) 1530
AcsI RAATTY 5 cut(s) 248, 663, 994, 1094, 1610
AcuI CTGAAG 4 cut(s) 104, 370, 714, 1459
AfaI GTAC 3 cut(s) 842, 1298, 1464
AfiI CCNNNNNNNGG 2 cut(s) 171, 824
AhlI ACTAGT 1 cut(s) 1436
AjnI CCWGG 3 cut(s) 897, 1164, 1270
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
Alw26I GTCTC 2 cut(s) 1015, 1068
AlwI GGATC 1 cut(s) 1530
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 1 cut(s) 744
ApeKI GCWGC 4 cut(s) 46, 1161, 1582, 1604
ApoI RAATTY 5 cut(s) 248, 663, 994, 1094, 1610
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 4 cut(s) 31, 696, 861, 1527
Asp718I GGTACC 1 cut(s) 1462
AspA2I CCTAGG 1 cut(s) 1046
AspS9I GGNCC 3 cut(s) 745, 901, 1136
AsuC2I CCSGG 3 cut(s) 438, 439, 768
AsuHPI GGTGA 2 cut(s) 254, 1313
AsuII TTCGAA 1 cut(s) 1082
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 2 cut(s) 901, 1136
AvrII CCTAGG 1 cut(s) 1046
BanI GGYRCC 1 cut(s) 1462
BbsI GAAGAC 3 cut(s) 129, 286, 1398
BbvI GCAGC 4 cut(s) 58, 1173, 1569, 1616
BccI CCATC 2 cut(s) 569, 878
BciT130I CCWGG 3 cut(s) 899, 1166, 1272
BciVI GTATCC 1 cut(s) 1569
BclI TGATCA 3 cut(s) 51, 138, 1318
BcnI CCSGG 3 cut(s) 438, 439, 768
BcoDI GTCTC 2 cut(s) 1015, 1068
BcuI ACTAGT 1 cut(s) 1436
BfaI CTAG 6 cut(s) 342, 927, 1047, 1113, 1251, 1437
BfuI GTATCC 1 cut(s) 1569
BglII AGATCT 2 cut(s) 352, 650
BisI GCNGC 6 cut(s) 47, 129, 1162, 1493, 1583, 1605
BlnI CCTAGG 1 cut(s) 1046
BlpI GCTNAGC 1 cut(s) 738
BlsI GCNGC 6 cut(s) 48, 130, 1163, 1494, 1584, 1606
Bme1390I CCNGG 6 cut(s) 438, 439, 768, 899, 1166, 1272
Bme18I GGWCC 2 cut(s) 901, 1136
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 3 cut(s) 745, 901, 1136
BmiI GGNNCC 4 cut(s) 443, 747, 1137, 1464
BmrFI CCNGG 6 cut(s) 438, 439, 768, 899, 1166, 1272
BmsI GCATC 2 cut(s) 549, 1129
BpiI GAAGAC 3 cut(s) 129, 286, 1398
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 1 cut(s) 738
Bpu14I TTCGAA 1 cut(s) 1082
BpuEI CTTGAG 2 cut(s) 59, 1607
BpuMI CCSGG 3 cut(s) 438, 439, 768
BsaJI CCNNGG 5 cut(s) 436, 437, 767, 818, 1046
BsaXI ACNNNNNCTCC 4 cut(s) 819, 849, 1633, 1663
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 824
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 624, 708
Bse3DI GCAATG 2 cut(s) 420, 1195
BseBI CCWGG 3 cut(s) 899, 1166, 1272
BseDI CCNNGG 5 cut(s) 436, 437, 767, 818, 1046
BseGI GGATG 1 cut(s) 1573
BseLI CCNNNNNNNGG 2 cut(s) 171, 824
BseMI GCAATG 2 cut(s) 420, 1195
BseMII CTCAG 3 cut(s) 351, 1079, 1669
BseNI ACTGG 2 cut(s) 624, 708
BseRI GAGGAG 1 cut(s) 1641
BseXI GCAGC 4 cut(s) 58, 1173, 1569, 1616
BsgI GTGCAG 2 cut(s) 83, 1180
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 746
BshNI GGYRCC 1 cut(s) 1462
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 4 cut(s) 70, 438, 767, 1496
BslFI GGGAC 2 cut(s) 460, 1149
BslI CCNNNNNNNGG 2 cut(s) 171, 824
BsmAI GTCTC 2 cut(s) 1015, 1068
BsmFI GGGAC 2 cut(s) 460, 1149
BsmI GAATGC 1 cut(s) 212
BsnI GGCC 1 cut(s) 746
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 1082
Bsp1407I TGTACA 2 cut(s) 840, 1296
Bsp143I GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
Bsp1720I GCTNAGC 1 cut(s) 738
BspACI CCGC 5 cut(s) 128, 131, 1334, 1493, 1516
BspANI GGCC 1 cut(s) 746
BspCNI CTCAG 3 cut(s) 352, 1078, 1668
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 2 cut(s) 700, 1276
BspLI GGNNCC 4 cut(s) 443, 747, 1137, 1464
BspPI GGATC 1 cut(s) 1530
BspQI GCTCTTC 1 cut(s) 389
BspT104I TTCGAA 1 cut(s) 1082
BspT107I GGYRCC 1 cut(s) 1462
BsrDI GCAATG 2 cut(s) 420, 1195
BsrFI RCCGGY 1 cut(s) 69
BsrGI TGTACA 2 cut(s) 840, 1296
BsrI ACTGG 2 cut(s) 624, 708
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 5 cut(s) 436, 437, 767, 818, 1046
BssMI GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
BssT1I CCWWGG 2 cut(s) 818, 1046
Bst2UI CCWGG 3 cut(s) 899, 1166, 1272
Bst4CI ACNGT 7 cut(s) 222, 231, 293, 474, 754, 1432, 1550
Bst6I CTCTTC 4 cut(s) 389, 852, 1046, 1518
BstAPI GCANNNNNTGC 1 cut(s) 419
BstAUI TGTACA 2 cut(s) 840, 1296
BstBI TTCGAA 1 cut(s) 1082
BstC8I GCNNGC 4 cut(s) 44, 71, 744, 1385
BstDEI CTNAG 5 cut(s) 360, 365, 738, 1065, 1655
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 1573
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 8 cut(s) 54, 141, 355, 653, 967, 1058, 1321, 1538
BstMAI GTCTC 2 cut(s) 1015, 1068
BstMBI GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
BstMWI GCNNNNNNNGC 8 cut(s) 70, 79, 156, 419, 743, 1161, 1333, 1645
BstNI CCWGG 3 cut(s) 899, 1166, 1272
BstSCI CCNGG 6 cut(s) 436, 437, 766, 897, 1164, 1270
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 58, 1173, 1569, 1616
BstV2I GAAGAC 3 cut(s) 129, 286, 1398
BstX2I RGATCY 2 cut(s) 352, 650
BstYI RGATCY 2 cut(s) 352, 650
BsuI GTATCC 1 cut(s) 1569
BsuRI GGCC 1 cut(s) 746
BtsCI GGATG 1 cut(s) 1573
BtsI GCAGTG 1 cut(s) 71
BtsIMutI CAGTG 4 cut(s) 71, 218, 617, 1065
Cac8I GCNNGC 4 cut(s) 44, 71, 744, 1385
CciI TCATGA 2 cut(s) 700, 1276
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 745, 901, 1136
Cfr9I CCCGGG 1 cut(s) 437
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 841, 1297, 1463
CspCI CAANNNNNGTGG 2 cut(s) 1236, 1271
CviAII CATG 7 cut(s) 701, 984, 1152, 1176, 1277, 1568, 1615
CviQI GTAC 3 cut(s) 841, 1297, 1463
DdeI CTNAG 5 cut(s) 360, 365, 738, 1065, 1655
DpnI GATC 8 cut(s) 53, 140, 354, 652, 966, 1057, 1320, 1537
DpnII GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
Eam1104I CTCTTC 4 cut(s) 389, 852, 1046, 1518
EarI CTCTTC 4 cut(s) 389, 852, 1046, 1518
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 818, 1046
Eco47I GGWCC 2 cut(s) 901, 1136
Eco57I CTGAAG 4 cut(s) 104, 370, 714, 1459
Eco88I CYCGRG 1 cut(s) 437
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 1136
EcoRI GAATTC 1 cut(s) 994
EcoRII CCWGG 3 cut(s) 897, 1164, 1270
EcoT14I CCWWGG 2 cut(s) 818, 1046
EcoT22I ATGCAT 1 cut(s) 212
ErhI CCWWGG 2 cut(s) 818, 1046
FaeI CATG 7 cut(s) 704, 987, 1155, 1179, 1280, 1571, 1618
FaqI GGGAC 2 cut(s) 460, 1149
FatI CATG 7 cut(s) 700, 983, 1151, 1175, 1276, 1567, 1614
FauNDI CATATG 1 cut(s) 975
FbaI TGATCA 3 cut(s) 51, 138, 1318
Fnu4HI GCNGC 6 cut(s) 47, 129, 1162, 1493, 1583, 1605
FokI GGATG 1 cut(s) 1560
Fsp4HI GCNGC 6 cut(s) 47, 129, 1162, 1493, 1583, 1605
FspBI CTAG 6 cut(s) 342, 927, 1047, 1113, 1251, 1437
GluI GCNGC 6 cut(s) 47, 129, 1162, 1493, 1583, 1605
HaeIII GGCC 1 cut(s) 746
HapII CCGG 4 cut(s) 70, 438, 767, 1496
HgaI GACGC 1 cut(s) 134
Hin1II CATG 7 cut(s) 704, 987, 1155, 1179, 1280, 1571, 1618
HincII GTYRAC 1 cut(s) 1507
HindII GTYRAC 1 cut(s) 1507
HindIII AAGCTT 2 cut(s) 30, 1385
HinfI GANTC 8 cut(s) 623, 704, 761, 812, 946, 1008, 1367, 1503
HpaII CCGG 4 cut(s) 70, 438, 767, 1496
HphI GGTGA 2 cut(s) 254, 1313
Hpy166II GTNNAC 5 cut(s) 451, 608, 841, 1297, 1507
Hpy188III TCNNGA 8 cut(s) 142, 425, 701, 950, 1005, 1067, 1277, 1624
Hpy8I GTNNAC 5 cut(s) 451, 608, 841, 1297, 1507
HpyAV CCTTC 6 cut(s) 149, 175, 269, 583, 671, 1078
HpyCH4III ACNGT 7 cut(s) 222, 231, 293, 474, 754, 1432, 1550
HpyCH4IV ACGT 2 cut(s) 394, 1633
HpyF10VI GCNNNNNNNGC 8 cut(s) 70, 79, 156, 419, 743, 1161, 1333, 1645
HpyF3I CTNAG 5 cut(s) 360, 365, 738, 1065, 1655
HpySE526I ACGT 2 cut(s) 394, 1633
Hsp92II CATG 7 cut(s) 704, 987, 1155, 1179, 1280, 1571, 1618
KpnI GGTACC 1 cut(s) 1466
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 3 cut(s) 51, 138, 1318
Kzo9I GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
LguI GCTCTTC 1 cut(s) 389
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 58, 1173, 1569, 1616
LweI GCATC 2 cut(s) 549, 1129
MaeI CTAG 6 cut(s) 342, 927, 1047, 1113, 1251, 1437
MaeII ACGT 2 cut(s) 394, 1633
MaeIII GTNAC 4 cut(s) 487, 1103, 1432, 1483
MalI GATC 8 cut(s) 53, 140, 354, 652, 966, 1057, 1320, 1537
MboI GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
MfeI CAATTG 1 cut(s) 113
MflI RGATCY 2 cut(s) 352, 650
MlyI GAGTC 3 cut(s) 755, 1017, 1512
MmeI TCCRAC 3 cut(s) 213, 1244, 1451
Mph1103I ATGCAT 1 cut(s) 212
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 4 cut(s) 31, 696, 861, 1527
MslI CAYNNNNRTG 1 cut(s) 1156
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 4 cut(s) 70, 438, 767, 1496
MspR9I CCNGG 6 cut(s) 438, 439, 768, 899, 1166, 1272
MunI CAATTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 212
MvaI CCWGG 3 cut(s) 899, 1166, 1272
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 8 cut(s) 70, 79, 156, 419, 743, 1161, 1333, 1645
NaeI GCCGGC 1 cut(s) 71
NciI CCSGG 3 cut(s) 438, 439, 768
NdeI CATATG 1 cut(s) 975
NdeII GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 7 cut(s) 704, 987, 1155, 1179, 1280, 1571, 1618
NlaIV GGNNCC 4 cut(s) 443, 747, 1137, 1464
NmuCI GTSAC 2 cut(s) 1432, 1483
NsiI ATGCAT 1 cut(s) 212
NspV TTCGAA 1 cut(s) 1082
PagI TCATGA 2 cut(s) 700, 1276
PciSI GCTCTTC 1 cut(s) 389
PctI GAATGC 1 cut(s) 212
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 4 cut(s) 31, 696, 861, 1527
PfeI GAWTC 5 cut(s) 623, 704, 812, 946, 1367
PflMI CCANNNNNTGG 1 cut(s) 824
PkrI GCNGC 6 cut(s) 48, 130, 1163, 1494, 1584, 1606
PleI GAGTC 3 cut(s) 755, 1016, 1511
PpsI GAGTC 3 cut(s) 755, 1016, 1511
PpuMI RGGWCCY 1 cut(s) 1136
Psp1406I AACGTT 1 cut(s) 394
Psp5II RGGWCCY 1 cut(s) 1136
Psp6I CCWGG 3 cut(s) 897, 1164, 1270
PspGI CCWGG 3 cut(s) 897, 1164, 1270
PspN4I GGNNCC 4 cut(s) 443, 747, 1137, 1464
PspPI GGNCC 3 cut(s) 745, 901, 1136
PspPPI RGGWCCY 1 cut(s) 1136
PsuI RGATCY 2 cut(s) 352, 650
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 3 cut(s) 842, 1298, 1464
RsaNI GTAC 3 cut(s) 841, 1297, 1463
RseI CAYNNNNRTG 1 cut(s) 1156
SapI GCTCTTC 1 cut(s) 389
SatI GCNGC 6 cut(s) 47, 129, 1162, 1493, 1583, 1605
Sau3AI GATC 8 cut(s) 51, 138, 352, 650, 964, 1055, 1318, 1535
Sau96I GGNCC 3 cut(s) 745, 901, 1136
SchI GAGTC 3 cut(s) 755, 1017, 1512
ScrFI CCNGG 6 cut(s) 438, 439, 768, 899, 1166, 1272
SfaNI GCATC 2 cut(s) 549, 1129
SfuI TTCGAA 1 cut(s) 1082
SinI GGWCC 2 cut(s) 901, 1136
SmaI CCCGGG 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 1156
SmlI CTYRAG 2 cut(s) 74, 1622
SmoI CTYRAG 2 cut(s) 74, 1622
SpeI ACTAGT 1 cut(s) 1436
SsiI CCGC 5 cut(s) 128, 131, 1334, 1493, 1516
SspMI CTAG 6 cut(s) 342, 927, 1047, 1113, 1251, 1437
StyD4I CCNGG 6 cut(s) 436, 437, 766, 897, 1164, 1270
StyI CCWWGG 2 cut(s) 818, 1046
TaaI ACNGT 7 cut(s) 222, 231, 293, 474, 754, 1432, 1550
TaiI ACGT 2 cut(s) 397, 1636
TaqI TCGA 4 cut(s) 564, 675, 1082, 1206
TatI WGTACW 2 cut(s) 840, 1296
TauI GCSGC 2 cut(s) 131, 1495
TfiI GAWTC 5 cut(s) 623, 704, 812, 946, 1367
TscAI CASTG 4 cut(s) 71, 225, 624, 1065
TseFI GTSAC 2 cut(s) 1432, 1483
TseI GCWGC 4 cut(s) 46, 1161, 1582, 1604
Tsp45I GTSAC 2 cut(s) 1432, 1483
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 4 cut(s) 71, 225, 624, 1065
Van91I CCANNNNNTGG 1 cut(s) 824
VpaK11BI GGWCC 2 cut(s) 901, 1136
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 5 cut(s) 248, 663, 994, 1094, 1610
XmaI CCCGGG 1 cut(s) 437
XmaJI CCTAGG 1 cut(s) 1046
XmnI GAANNNNTTC 4 cut(s) 31, 696, 861, 1527
XspI CTAG 6 cut(s) 342, 927, 1047, 1113, 1251, 1437
Zsp2I ATGCAT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.