Rh2BG577000

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013512
Physical Location & Seq
Forward (+)
79356992 .. 79358255
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG577000.1

Sequence Viewer

Length: 384 bp
ATGTTTGACATATTGTTCGGGTGGAGAAAAGCTTCAAAGTGCAAGCAGCTGATCAAACAGGTGCAGTGCCGGCTCAAGTTGCTGAAGAACAAGAGGGCTACTATTGTGAGGCAATTGCGAGAAGACGCGGCGGAGCTGATCAAGAATGGCTACGAAGGCAAAGCCTTCAATAGGGTTGAGCAGATAATCAAAGATGAATGCATAGTGGCACTGTATGAACTGTTGGATAATTTCTGTGAATTTATCCTCCTTCACCTTTCATATATCCGAAGACACAAATTGGTCAATGAAATAGCAAGGAATTACTGCATCAAACCAGAGGTTTTAGCCATCGAATACTATTCCGATTGGCAACAGAAGGTGAGTGACTACATACAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

127

Amino Acids

15.21

Weight (kDa)

9.1

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 97 4.1e-19 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 128
AciI CCGC 2 cut(s) 128, 131
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 2 cut(s) 36, 169
AluBI AGCT 4 cut(s) 32, 49, 136, 379
AluI AGCT 4 cut(s) 32, 49, 136, 379
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 239
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 31
AsuHPI GGTGA 2 cut(s) 245, 373
BbsI GAAGAC 2 cut(s) 129, 277
BbvI GCAGC 1 cut(s) 58
BccI CCATC 1 cut(s) 338
BclI TGATCA 2 cut(s) 51, 138
BisI GCNGC 2 cut(s) 47, 129
BlsI GCNGC 2 cut(s) 48, 130
BmsI GCATC 1 cut(s) 318
BpiI GAAGAC 2 cut(s) 129, 277
BpuEI CTTGAG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse118I RCCGGY 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 171
BsmI GAATGC 1 cut(s) 203
Bsp143I GATC 2 cut(s) 51, 138
BspACI CCGC 2 cut(s) 128, 131
BspFNI CGCG 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 69
BssMI GATC 2 cut(s) 51, 138
Bst4CI ACNGT 2 cut(s) 213, 222
BstC8I GCNNGC 2 cut(s) 44, 71
BstENI CCTNNNNNAGG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 2 cut(s) 54, 141
BstMBI GATC 2 cut(s) 51, 138
BstMWI GCNNNNNNNGC 3 cut(s) 70, 79, 156
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 58
BstV2I GAAGAC 2 cut(s) 129, 277
BtsI GCAGTG 1 cut(s) 71
BtsIMutI CAGTG 2 cut(s) 71, 209
Cac8I GCNNGC 2 cut(s) 44, 71
Cfr10I RCCGGY 1 cut(s) 69
CseI GACGC 1 cut(s) 134
CviJI RGCY 9 cut(s) 32, 49, 73, 98, 136, 150, 164, 329, 379
CviKI_1 RGCY 9 cut(s) 32, 49, 73, 98, 136, 150, 164, 329, 379
DpnI GATC 2 cut(s) 53, 140
DpnII GATC 2 cut(s) 51, 138
EciI GGCGGA 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 104
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 203
FaiI YATR 6 cut(s) 11, 203, 216, 262, 264, 374
FbaI TGATCA 2 cut(s) 51, 138
Fnu4HI GCNGC 2 cut(s) 47, 129
Fsp4HI GCNGC 2 cut(s) 47, 129
GluI GCNGC 2 cut(s) 47, 129
HapII CCGG 1 cut(s) 70
HgaI GACGC 1 cut(s) 134
HindIII AAGCTT 1 cut(s) 30
HpaII CCGG 1 cut(s) 70
HphI GGTGA 2 cut(s) 245, 373
Hpy188I TCNGA 2 cut(s) 269, 346
Hpy188III TCNNGA 1 cut(s) 142
HpyAV CCTTC 4 cut(s) 149, 175, 260, 352
HpyCH4III ACNGT 2 cut(s) 213, 222
HpyCH4V TGCA 4 cut(s) 42, 64, 201, 309
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 79, 156
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 2 cut(s) 51, 138
Kzo9I GATC 2 cut(s) 51, 138
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 3 cut(s) 44, 83, 330
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 365
MalI GATC 2 cut(s) 53, 140
MboI GATC 2 cut(s) 51, 138
MboII GAAGA 3 cut(s) 97, 134, 282
MfeI CAATTG 1 cut(s) 113
MluCI AATT 5 cut(s) 113, 229, 239, 278, 301
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 4 cut(s) 87, 102, 257, 313
Mph1103I ATGCAT 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 31
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 203
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 3 cut(s) 70, 79, 156
NaeI GCCGGC 1 cut(s) 71
NdeII GATC 2 cut(s) 51, 138
NgoMIV GCCGGC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 365
NsiI ATGCAT 1 cut(s) 203
PctI GAATGC 1 cut(s) 203
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 31
PkrI GCNGC 2 cut(s) 48, 130
PvuII CAGCTG 1 cut(s) 49
SatI GCNGC 2 cut(s) 47, 129
Sau3AI GATC 2 cut(s) 51, 138
SetI ASST 8 cut(s) 34, 51, 63, 138, 258, 324, 363, 381
SfaNI GCATC 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 5 cut(s) 113, 229, 239, 278, 301
SsiI CCGC 2 cut(s) 128, 131
TaaI ACNGT 2 cut(s) 213, 222
TaqI TCGA 1 cut(s) 333
TasI AATT 5 cut(s) 113, 229, 239, 278, 301
TauI GCSGC 1 cut(s) 131
TscAI CASTG 2 cut(s) 71, 216
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 4 cut(s) 210, 231, 249, 303
TspRI CASTG 2 cut(s) 71, 216
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.