FvH4_6g45140

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013512
Physical Location & Seq
Forward (+)
34661281 .. 34664919
3639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45140.t1

Sequence Viewer

Length: 1650 bp
ATGTTTGACATCTTGTTCGGGTGGAGAAAAGCTTCGAAGTGCAAGCAGCTGATCAAGCAGGTGCAGTGCCGGCTCAAGTTGCTGAAGAACAAGAGGGCAACTATTGTGAGGCAATTGCGAGAAGACGCAGCGGAGCTGATCAAGAATGGCTACGAAGACAAAGCCTTCAATAGGGTTGAGCAGATAATCAAAGACGAATGTATTGTGGCACTGTATGAACTGTTGGACAATTTCTGTGAATTCATCCTCCTTCACCTTTCATATATCCGAAGACGCAAGGACTGTCCAAATGACATAAATGAAGCAGTTTCAAGTCTTATATATGCTTCTGCTAGATGTGGAGACCTTCCTGAGCTTAGAACAATCCGAAAGCTCTTCGGAGAACGTTATGGCCTGAAATTTGCAATGACTGCTCTTGAACTATTCCCTGGGAACCTTGTGAACTGTCAGGTAATAGAGAAACTGACGCTAAAGCCAGTATCAGATGATATGAAACATAGATTCGTGAATGAAATAGCAAGGAATTACTGCATCAAACCAGAGGTTTTAGCAATTGAATACTATTCTGATTGGCAACAGAAGGTGAAGGAAACTAGTGGACATCTAGCACTGGATTCAAATGTTCGGACTTATTACGAAAGATCTGAAATGCAAATTTGCAAGGTGGAAGAGCTTCAGACAGAAGATGTTCATGATTCCAATATATCTTGTACTACTTCAGTACCTAGGCTAGCCCCGACTATATTAGACTCTCCACTGCATGATGAAATACAGGACCTCTATGCTGAAGTGGATTCTCCAAGAGGTGGAGAAATAGTGGTGTACAATGATGACATTGAAGAGGTTCGGTCTTGCACAACAAGAGATGGAGATGACTTCCAAGACCAAAGAGCTTTCAAGTTCAAAGCTAGCGTCGCCAAAAAAGAGAATCTTGAAAACAGTTTTGATCATAGTTATGTGGCGACATGGAGTGTGAATTCAGGATCAGGAGTCTCTAGGAGAAGTTTTAAGGGAAATGAGAAAAGACCAAGGAAGAGATCAGTGTCCCAGGAAAATCAGTTTTTGAAGGATAATGAATTTCCAAGTTACTATGCAAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTGGTAAAACCATGTTATGTTTGCAATGGGAAAGAGCATGTCGAATTTGAAGTTCCGACACTGAAGACAAATAGAGGGATTTCAACTAGGGTGGGATTTGCTAATCCGGGTCATGAACAGAAAAGTTCGGTGTACAATGCTTTCACTTACCCTGATCACCAGCCAAAAACGCAACGCAAAGTGTTCAAAGGAACCATAGAGGAATCCGATTTTCGACGCAAGCTTTCCTCTTCTAGTTCGTCTTCATCATCATCTTCTCCACCCATGACTAGTTCTTGGATGAGAAAAGAAATGGTACCTCCTTACTTCAGAAACGTGACAATGCCACCGGAAAGAGTCATGGACAAGCGGAAAGAAGAGTTCCAACGATCCATTTCAGACTGTTTTCAGTATCCTACTCATGTTCATCCGAACCTGCCAGATTACGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAACCTAAGCCTCATCGCAACAATCAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

550

Amino Acids

63.58

Weight (kDa)

8.84

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 178 9.4e-52 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 49
Acc36I ACCTGC 2 cut(s) 49, 1569
Acc65I GGTACC 1 cut(s) 1441
AccB1I GGYRCC 1 cut(s) 1441
AccB7I CCANNNNNTGG 1 cut(s) 806
AciI CCGC 2 cut(s) 131, 1495
AclI AACGTT 1 cut(s) 385
AclWI GGATC 2 cut(s) 991, 1509
AcsI RAATTY 7 cut(s) 239, 398, 654, 976, 1076, 1190, 1589
AcuI CTGAAG 6 cut(s) 104, 659, 702, 807, 1229, 1438
AfaI GTAC 5 cut(s) 712, 723, 824, 1280, 1443
AfiI CCNNNNNNNGG 2 cut(s) 171, 806
AhlI ACTAGT 2 cut(s) 593, 1415
AjnI CCWGG 3 cut(s) 427, 1047, 1146
Alw26I GTCTC 2 cut(s) 336, 997
AlwI GGATC 2 cut(s) 991, 1509
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 4 cut(s) 46, 128, 1143, 1583
ApoI RAATTY 7 cut(s) 239, 398, 654, 976, 1076, 1190, 1589
ArsI GACNNNNNNTTYG 3 cut(s) 31, 897, 929
Asp700I GAANNNNTTC 4 cut(s) 31, 672, 687, 843
Asp718I GGTACC 1 cut(s) 1441
AspA2I CCTAGG 1 cut(s) 725
AspS9I GGNCC 2 cut(s) 775, 1118
AsuC2I CCSGG 1 cut(s) 1254
AsuHPI GGTGA 3 cut(s) 245, 595, 1295
AsuII TTCGAA 1 cut(s) 35
AsuNHI GCTAGC 2 cut(s) 730, 908
AvaII GGWCC 2 cut(s) 775, 1118
AvrII CCTAGG 1 cut(s) 725
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
BanI GGYRCC 1 cut(s) 1441
BbsI GAAGAC 5 cut(s) 129, 162, 277, 1217, 1380
BbvI GCAGC 4 cut(s) 58, 140, 1155, 1595
BccI CCATC 1 cut(s) 860
BcgI CGANNNNNNTGC 2 cut(s) 1562, 1596
BciT130I CCWGG 3 cut(s) 429, 1049, 1148
BciVI GTATCC 1 cut(s) 1548
BclI TGATCA 4 cut(s) 51, 138, 946, 1300
BcnI CCSGG 1 cut(s) 1254
BcoDI GTCTC 2 cut(s) 336, 997
BcuI ACTAGT 2 cut(s) 593, 1415
BfuAI ACCTGC 2 cut(s) 49, 1569
BfuI GTATCC 1 cut(s) 1548
BglII AGATCT 1 cut(s) 641
BisI GCNGC 4 cut(s) 47, 129, 1144, 1584
BlnI CCTAGG 1 cut(s) 725
BlsI GCNGC 4 cut(s) 48, 130, 1145, 1585
Bme1390I CCNGG 4 cut(s) 429, 1049, 1148, 1254
Bme18I GGWCC 2 cut(s) 775, 1118
BmgT120I GGNCC 2 cut(s) 775, 1118
BmiI GGNNCC 4 cut(s) 434, 1119, 1339, 1443
BmrFI CCNGG 4 cut(s) 429, 1049, 1148, 1254
BmsI GCATC 2 cut(s) 540, 1111
BmtI GCTAGC 2 cut(s) 734, 912
BpiI GAAGAC 5 cut(s) 129, 162, 277, 1217, 1380
Bpu10I CCTNAGC 2 cut(s) 351, 1623
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 2 cut(s) 59, 1586
BpuMI CCSGG 1 cut(s) 1254
BsaI GGTCTC 1 cut(s) 336
BsaJI CCNNGG 5 cut(s) 427, 428, 725, 1028, 1047
BsaWI WCCGGW 1 cut(s) 1474
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 806
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 476, 615
Bse3DI GCAATG 2 cut(s) 411, 1177
BseBI CCWGG 3 cut(s) 429, 1049, 1148
BseDI CCNNGG 5 cut(s) 427, 428, 725, 1028, 1047
BseGI GGATG 3 cut(s) 243, 1431, 1552
BseLI CCNNNNNNNGG 2 cut(s) 171, 806
BseMI GCAATG 2 cut(s) 411, 1177
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 2 cut(s) 476, 615
BseXI GCAGC 4 cut(s) 58, 140, 1155, 1595
BsgI GTGCAG 2 cut(s) 83, 1162
BshFI GGCC 1 cut(s) 393
BshNI GGYRCC 1 cut(s) 1441
BsiSI CCGG 3 cut(s) 70, 1253, 1475
BslFI GGGAC 2 cut(s) 1030, 1131
BslI CCNNNNNNNGG 2 cut(s) 171, 806
BsmAI GTCTC 2 cut(s) 336, 997
BsmFI GGGAC 2 cut(s) 1030, 1131
BsnI GGCC 1 cut(s) 393
Bso31I GGTCTC 1 cut(s) 336
Bsp119I TTCGAA 1 cut(s) 35
Bsp1407I TGTACA 2 cut(s) 822, 1278
Bsp143I GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
BspACI CCGC 2 cut(s) 131, 1495
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 1 cut(s) 343
BspHI TCATGA 2 cut(s) 691, 1258
BspLI GGNNCC 4 cut(s) 434, 1119, 1339, 1443
BspMI ACCTGC 2 cut(s) 49, 1569
BspOI GCTAGC 2 cut(s) 734, 912
BspPI GGATC 2 cut(s) 991, 1509
BspQI GCTCTTC 2 cut(s) 380, 663
BspT104I TTCGAA 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 1441
BspTNI GGTCTC 1 cut(s) 336
BsrDI GCAATG 2 cut(s) 411, 1177
BsrFI RCCGGY 1 cut(s) 69
BsrGI TGTACA 2 cut(s) 822, 1278
BsrI ACTGG 2 cut(s) 476, 615
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 5 cut(s) 427, 428, 725, 1028, 1047
BssMI GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
BssT1I CCWWGG 2 cut(s) 725, 1028
Bst2UI CCWGG 3 cut(s) 429, 1049, 1148
Bst4CI ACNGT 6 cut(s) 213, 222, 284, 446, 941, 1529
Bst6I CTCTTC 6 cut(s) 380, 663, 834, 1028, 1381, 1497
BstAPI GCANNNNNTGC 1 cut(s) 410
BstAUI TGTACA 2 cut(s) 822, 1278
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 5 cut(s) 44, 71, 732, 910, 1367
BstDEI CTNAG 3 cut(s) 351, 356, 1623
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 3 cut(s) 243, 1431, 1552
BstKTI GATC 8 cut(s) 54, 141, 644, 949, 986, 1040, 1303, 1517
BstMAI GTCTC 2 cut(s) 336, 997
BstMBI GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
BstMWI GCNNNNNNNGC 8 cut(s) 55, 70, 79, 410, 914, 1143, 1315, 1624
BstNI CCWGG 3 cut(s) 429, 1049, 1148
BstNSI RCATGY 1 cut(s) 1187
BstSCI CCNGG 4 cut(s) 427, 1047, 1146, 1252
BstV1I GCAGC 4 cut(s) 58, 140, 1155, 1595
BstV2I GAAGAC 5 cut(s) 129, 162, 277, 1217, 1380
BstX2I RGATCY 1 cut(s) 641
BstYI RGATCY 1 cut(s) 641
BsuI GTATCC 1 cut(s) 1548
BsuRI GGCC 1 cut(s) 393
BtgZI GCGATG 1 cut(s) 1616
BtsCI GGATG 3 cut(s) 243, 1431, 1552
BtsI GCAGTG 2 cut(s) 71, 755
BtsIMutI CAGTG 6 cut(s) 71, 209, 608, 755, 1047, 1205
BveI ACCTGC 2 cut(s) 49, 1569
Cac8I GCNNGC 5 cut(s) 44, 71, 732, 910, 1367
CciI TCATGA 2 cut(s) 691, 1258
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 775, 1118
CseI GACGC 5 cut(s) 134, 282, 475, 901, 1371
Csp6I GTAC 5 cut(s) 711, 722, 823, 1279, 1442
CspCI CAANNNNNGTGG 2 cut(s) 1218, 1253
CviQI GTAC 5 cut(s) 711, 722, 823, 1279, 1442
DdeI CTNAG 3 cut(s) 351, 356, 1623
DpnI GATC 8 cut(s) 53, 140, 643, 948, 985, 1039, 1302, 1516
DpnII GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
Eam1104I CTCTTC 6 cut(s) 380, 663, 834, 1028, 1381, 1497
EarI CTCTTC 6 cut(s) 380, 663, 834, 1028, 1381, 1497
Eco130I CCWWGG 2 cut(s) 725, 1028
Eco31I GGTCTC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 775, 1118
Eco57I CTGAAG 6 cut(s) 104, 659, 702, 807, 1229, 1438
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoO109I RGGNCCY 2 cut(s) 775, 1118
EcoRI GAATTC 2 cut(s) 239, 976
EcoRII CCWGG 3 cut(s) 427, 1047, 1146
EcoT14I CCWWGG 2 cut(s) 725, 1028
ErhI CCWWGG 2 cut(s) 725, 1028
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 2 cut(s) 1030, 1131
FbaI TGATCA 4 cut(s) 51, 138, 946, 1300
Fnu4HI GCNGC 4 cut(s) 47, 129, 1144, 1584
FokI GGATG 3 cut(s) 230, 1438, 1539
Fsp4HI GCNGC 4 cut(s) 47, 129, 1144, 1584
GluI GCNGC 4 cut(s) 47, 129, 1144, 1584
HaeIII GGCC 1 cut(s) 393
HapII CCGG 3 cut(s) 70, 1253, 1475
HgaI GACGC 5 cut(s) 134, 282, 475, 901, 1371
HindIII AAGCTT 2 cut(s) 30, 1367
HinfI GANTC 9 cut(s) 501, 614, 695, 749, 794, 928, 990, 1349, 1482
HpaII CCGG 3 cut(s) 70, 1253, 1475
HphI GGTGA 3 cut(s) 245, 595, 1295
Hpy166II GTNNAC 4 cut(s) 442, 599, 823, 1279
Hpy8I GTNNAC 4 cut(s) 442, 599, 823, 1279
Hpy99I CGWCG 2 cut(s) 917, 1365
HpyAV CCTTC 6 cut(s) 175, 260, 356, 574, 580, 1060
HpyCH4III ACNGT 6 cut(s) 213, 222, 284, 446, 941, 1529
HpyCH4IV ACGT 3 cut(s) 385, 1461, 1612
HpyF10VI GCNNNNNNNGC 8 cut(s) 55, 70, 79, 410, 914, 1143, 1315, 1624
HpyF3I CTNAG 3 cut(s) 351, 356, 1623
HpySE526I ACGT 3 cut(s) 385, 1461, 1612
KpnI GGTACC 1 cut(s) 1445
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 4 cut(s) 51, 138, 946, 1300
Kzo9I GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
LguI GCTCTTC 2 cut(s) 380, 663
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 58, 140, 1155, 1595
LweI GCATC 2 cut(s) 540, 1111
MaeII ACGT 3 cut(s) 385, 1461, 1612
MaeIII GTNAC 2 cut(s) 1085, 1462
MalI GATC 8 cut(s) 53, 140, 643, 948, 985, 1039, 1302, 1516
MboI GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
MfeI CAATTG 2 cut(s) 113, 552
MflI RGATCY 1 cut(s) 641
MlyI GAGTC 3 cut(s) 743, 999, 1491
MmeI TCCRAC 3 cut(s) 204, 1226, 1534
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 4 cut(s) 31, 672, 687, 843
MseI TTAA 2 cut(s) 1008, 1648
MslI CAYNNNNRTG 2 cut(s) 954, 1138
MspA1I CMGCKG 2 cut(s) 49, 131
MspI CCGG 3 cut(s) 70, 1253, 1475
MspR9I CCNGG 4 cut(s) 429, 1049, 1148, 1254
MunI CAATTG 2 cut(s) 113, 552
MvaI CCWGG 3 cut(s) 429, 1049, 1148
MwoI GCNNNNNNNGC 8 cut(s) 55, 70, 79, 410, 914, 1143, 1315, 1624
NaeI GCCGGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 1254
NdeII GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
NgoMIV GCCGGC 1 cut(s) 69
NheI GCTAGC 2 cut(s) 730, 908
NlaIV GGNNCC 4 cut(s) 434, 1119, 1339, 1443
NmuCI GTSAC 1 cut(s) 1462
NspI RCATGY 1 cut(s) 1187
NspV TTCGAA 1 cut(s) 35
PagI TCATGA 2 cut(s) 691, 1258
PaqCI CACCTGC 1 cut(s) 49
PasI CCCWGGG 1 cut(s) 428
PciSI GCTCTTC 2 cut(s) 380, 663
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 4 cut(s) 31, 672, 687, 843
PfeI GAWTC 6 cut(s) 501, 614, 695, 794, 928, 1349
PflMI CCANNNNNTGG 1 cut(s) 806
PkrI GCNGC 4 cut(s) 48, 130, 1145, 1585
PleI GAGTC 3 cut(s) 743, 998, 1490
PpsI GAGTC 3 cut(s) 743, 998, 1490
PpuMI RGGWCCY 2 cut(s) 775, 1118
Psp1406I AACGTT 1 cut(s) 385
Psp5II RGGWCCY 2 cut(s) 775, 1118
Psp6I CCWGG 3 cut(s) 427, 1047, 1146
PspGI CCWGG 3 cut(s) 427, 1047, 1146
PspN4I GGNNCC 4 cut(s) 434, 1119, 1339, 1443
PspPI GGNCC 2 cut(s) 775, 1118
PspPPI RGGWCCY 2 cut(s) 775, 1118
PsuI RGATCY 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 5 cut(s) 712, 723, 824, 1280, 1443
RsaNI GTAC 5 cut(s) 711, 722, 823, 1279, 1442
RseI CAYNNNNRTG 2 cut(s) 954, 1138
SapI GCTCTTC 2 cut(s) 380, 663
SaqAI TTAA 2 cut(s) 1008, 1648
SatI GCNGC 4 cut(s) 47, 129, 1144, 1584
Sau3AI GATC 8 cut(s) 51, 138, 641, 946, 983, 1037, 1300, 1514
Sau96I GGNCC 2 cut(s) 775, 1118
SchI GAGTC 3 cut(s) 743, 999, 1491
ScrFI CCNGG 4 cut(s) 429, 1049, 1148, 1254
SfaNI GCATC 2 cut(s) 540, 1111
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 2 cut(s) 775, 1118
SmiMI CAYNNNNRTG 2 cut(s) 954, 1138
SmlI CTYRAG 2 cut(s) 74, 1601
SmoI CTYRAG 2 cut(s) 74, 1601
SpeI ACTAGT 2 cut(s) 593, 1415
SsiI CCGC 2 cut(s) 131, 1495
StyD4I CCNGG 4 cut(s) 427, 1047, 1146, 1252
StyI CCWWGG 2 cut(s) 725, 1028
TaaI ACNGT 6 cut(s) 213, 222, 284, 446, 941, 1529
TaiI ACGT 3 cut(s) 388, 1464, 1615
TaqI TCGA 3 cut(s) 35, 1188, 1360
TaqII GACCGA 1 cut(s) 837
TatI WGTACW 3 cut(s) 710, 822, 1278
TfiI GAWTC 6 cut(s) 501, 614, 695, 794, 928, 1349
Tru1I TTAA 2 cut(s) 1008, 1648
Tru9I TTAA 2 cut(s) 1008, 1648
TscAI CASTG 6 cut(s) 71, 216, 615, 762, 1047, 1212
TseFI GTSAC 1 cut(s) 1462
TseI GCWGC 4 cut(s) 46, 128, 1143, 1583
Tsp45I GTSAC 1 cut(s) 1462
TspRI CASTG 6 cut(s) 71, 216, 615, 762, 1047, 1212
Van91I CCANNNNNTGG 1 cut(s) 806
VpaK11BI GGWCC 2 cut(s) 775, 1118
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 7 cut(s) 239, 398, 654, 976, 1076, 1190, 1589
XceI RCATGY 1 cut(s) 1187
XmaJI CCTAGG 1 cut(s) 725
XmnI GAANNNNTTC 4 cut(s) 31, 672, 687, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.