Prupe.5G187400_v2.0.a1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013512
Physical Location & Seq
Reverse (-)
15461393 .. 15465811
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G187400.1

Sequence Viewer

Length: 831 bp
ATGTCCGAAGTGAAGCCTTTTCAAAGGGCATTGTTGTATCAGTCATCCGATAAGGGCCTTAGAGTGTGTGTGAATGACTTTGCCGCCGATGTTGGTACAGATGGTGTCCGAATATGCCTCTTCCACTACTCGAATGAGGTTCACTTGTTCATAAGAGTTGTTCCTCCCAATGTCTACGGCAATTGGATAAGTATTGAATCCTCCCAAGGACAAACATTGAAGCCGGTTCAGGTTCAGTCCTGTGACCAAACCAAGAGGCAGACAATTGTGAGGCAATTGCGCGAAGACGTGGCGGAGCTGATCAAACATGGCCACGAAGACAAAGCCTTCAACAGGGTTGGACAGATAATCAAAGATGAATGCATAGTGGCAGTGTATGAATTGTTGGACAATTTCTGTGAATTCATCCTCATCCATCTTCCTTACATCCGCAGACACAAGGACTGTCCAAATGACATTAACGAAGCAGTTTCGAGTCTTATATATGCTTCTGCAAGATGTGGGGATCTCCCTGAGCTTAGGGTGATCAGGAAGCTCTTTGGTGAACGATATGGGCAGAAATTTGCAGTAACTGCTCTTGAACTATTCCCTGGGAATCTTGTGAACCATCAGTTGATTGAGAAACTCTCCCCAAAGTCAGTGACAGATGATATGAAGCAAAGATTGGTCAATGAAATAGCCAGGAATTACTGCATCAAACCAGAGGTCTTAGCAATTGAGTACTATTCTGAATGGCAACAAAAGGTGAAAGAAATTAGGGACTTAGAGTACTTGATACAGATGTTCAGACATATTATGAAGGAACTGAAAGATCTGAATTGCAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

277

Amino Acids

32.07

Weight (kDa)

7.06

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 282
AciI CCGC 3 cut(s) 84, 293, 430
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 2 cut(s) 401, 560
AfaI GTAC 3 cut(s) 97, 722, 770
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 5 cut(s) 23, 197, 220, 331, 581
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 2 cut(s) 589, 680
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 3 cut(s) 298, 517, 535
AluI AGCT 3 cut(s) 298, 517, 535
AlwI GGATC 1 cut(s) 513
AlwNI CAGNNNCTG 1 cut(s) 572
AoxI GGCC 2 cut(s) 55, 310
ApoI RAATTY 2 cut(s) 401, 560
ArsI GACNNNNNNTTYG 2 cut(s) 690, 722
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 535, 554, 757
BaeI ACNNNNGTAYC 2 cut(s) 767, 800
BalI TGGCCA 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 291, 324
BccI CCATC 3 cut(s) 95, 423, 615
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 2 cut(s) 591, 682
BclI TGATCA 2 cut(s) 300, 525
BglII AGATCT 1 cut(s) 811
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
BmcAI AGTACT 2 cut(s) 722, 770
Bme1390I CCNGG 2 cut(s) 591, 682
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 591, 682
BmsI GCATC 1 cut(s) 702
BpiI GAAGAC 2 cut(s) 291, 324
BplI GAGNNNNNCTC 2 cut(s) 611, 643
Bpu10I CCTNAGC 2 cut(s) 513, 518
BsaJI CCNNGG 3 cut(s) 205, 589, 590
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse118I RCCGGY 1 cut(s) 223
BseBI CCWGG 2 cut(s) 591, 682
BseDI CCNNGG 3 cut(s) 205, 589, 590
BseGI GGATG 4 cut(s) 44, 405, 411, 426
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 57, 312
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 773
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 2 cut(s) 57, 312
Bsp143I GATC 4 cut(s) 300, 505, 525, 811
BspACI CCGC 3 cut(s) 84, 293, 430
BspANI GGCC 2 cut(s) 57, 312
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 282
BspPI GGATC 1 cut(s) 513
BsrFI RCCGGY 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 3 cut(s) 205, 589, 590
BssMI GATC 4 cut(s) 300, 505, 525, 811
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 2 cut(s) 591, 682
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 572
BstDEI CTNAG 5 cut(s) 59, 513, 518, 709, 763
BstENI CCTNNNNNAGG 1 cut(s) 331
BstF5I GGATG 4 cut(s) 44, 405, 411, 426
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 4 cut(s) 303, 508, 528, 814
BstMBI GATC 4 cut(s) 300, 505, 525, 811
BstMWI GCNNNNNNNGC 1 cut(s) 572
BstNI CCWGG 2 cut(s) 591, 682
BstSCI CCNGG 2 cut(s) 589, 680
BstUI CGCG 1 cut(s) 282
BstV2I GAAGAC 2 cut(s) 291, 324
BstX2I RGATCY 2 cut(s) 505, 811
BstYI RGATCY 2 cut(s) 505, 811
BsuRI GGCC 2 cut(s) 57, 312
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 4 cut(s) 44, 405, 411, 426
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 2 cut(s) 378, 645
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 1 cut(s) 282
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 55
Csp6I GTAC 3 cut(s) 96, 721, 769
CviAII CATG 1 cut(s) 308
CviJI RGCY 9 cut(s) 16, 57, 223, 298, 312, 326, 517, 535, 680
CviKI_1 RGCY 9 cut(s) 16, 57, 223, 298, 312, 326, 517, 535, 680
CviQI GTAC 3 cut(s) 96, 721, 769
DdeI CTNAG 5 cut(s) 59, 513, 518, 709, 763
DpnI GATC 4 cut(s) 302, 507, 527, 813
DpnII GATC 4 cut(s) 300, 505, 525, 811
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
EciI GGCGGA 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 205
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 589, 680
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 365
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 1 cut(s) 311
FaqI GGGAC 1 cut(s) 773
FatI CATG 1 cut(s) 307
FbaI TGATCA 2 cut(s) 300, 525
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 4 cut(s) 31, 392, 398, 413
Fsp4HI GCNGC 1 cut(s) 84
GlaI GCGC 1 cut(s) 281
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 2 cut(s) 57, 312
HapII CCGG 1 cut(s) 224
HhaI GCGC 1 cut(s) 282
Hin1II CATG 1 cut(s) 311
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HinfI GANTC 3 cut(s) 197, 475, 595
HpaII CCGG 1 cut(s) 224
HphI GGTGA 3 cut(s) 535, 554, 757
Hpy166II GTNNAC 4 cut(s) 142, 175, 545, 604
Hpy188I TCNGA 6 cut(s) 7, 49, 110, 730, 788, 816
Hpy188III TCNNGA 2 cut(s) 529, 578
Hpy8I GTNNAC 4 cut(s) 142, 175, 545, 604
HpyAV CCTTC 2 cut(s) 337, 793
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 5 cut(s) 363, 494, 566, 693, 822
HpyF10VI GCNNNNNNNGC 1 cut(s) 572
HpyF3I CTNAG 5 cut(s) 59, 513, 518, 709, 763
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 1 cut(s) 311
HspAI GCGC 1 cut(s) 280
Ksp22I TGATCA 2 cut(s) 300, 525
Kzo9I GATC 4 cut(s) 300, 505, 525, 811
LmnI GCTCC 1 cut(s) 295
LweI GCATC 1 cut(s) 702
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 242, 568, 640
MalI GATC 4 cut(s) 302, 507, 527, 813
MboI GATC 4 cut(s) 300, 505, 525, 811
MboII GAAGA 4 cut(s) 112, 296, 329, 410
MfeI CAATTG 4 cut(s) 181, 264, 275, 714
MflI RGATCY 2 cut(s) 505, 811
MlsI TGGCCA 1 cut(s) 312
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 484
MmeI TCCRAC 2 cut(s) 319, 366
MnlI CCTC 8 cut(s) 128, 130, 174, 211, 249, 264, 419, 697
Mox20I TGGCCA 1 cut(s) 312
Mph1103I ATGCAT 1 cut(s) 365
MscI TGGCCA 1 cut(s) 312
MseI TTAA 1 cut(s) 459
Msp20I TGGCCA 1 cut(s) 312
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 591, 682
MunI CAATTG 4 cut(s) 181, 264, 275, 714
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 2 cut(s) 591, 682
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 572
NdeII GATC 4 cut(s) 300, 505, 525, 811
NlaIII CATG 1 cut(s) 311
NmuCI GTSAC 2 cut(s) 242, 640
NsiI ATGCAT 1 cut(s) 365
PasI CCCWGGG 1 cut(s) 590
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 197, 595
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
Psp6I CCWGG 2 cut(s) 589, 680
PspGI CCWGG 2 cut(s) 589, 680
PspPI GGNCC 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 2 cut(s) 505, 811
RsaI GTAC 3 cut(s) 97, 722, 770
RsaNI GTAC 3 cut(s) 96, 721, 769
SaqAI TTAA 1 cut(s) 459
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 4 cut(s) 300, 505, 525, 811
Sau96I GGNCC 1 cut(s) 55
ScaI AGTACT 2 cut(s) 722, 770
SchI GAGTC 1 cut(s) 484
ScrFI CCNGG 2 cut(s) 591, 682
SetI ASST 8 cut(s) 141, 234, 291, 300, 519, 537, 708, 747
SfaNI GCATC 1 cut(s) 702
SsiI CCGC 3 cut(s) 84, 293, 430
StyD4I CCNGG 2 cut(s) 589, 680
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 1 cut(s) 291
TaqI TCGA 2 cut(s) 131, 473
TatI WGTACW 2 cut(s) 720, 768
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 197, 595
Tru1I TTAA 1 cut(s) 459
Tru9I TTAA 1 cut(s) 459
TscAI CASTG 2 cut(s) 378, 645
TseFI GTSAC 2 cut(s) 242, 640
Tsp45I GTSAC 2 cut(s) 242, 640
TspDTI ATGAA 7 cut(s) 139, 372, 393, 394, 668, 687, 812
TspRI CASTG 2 cut(s) 378, 645
XagI CCTNNNNNAGG 1 cut(s) 331
XapI RAATTY 2 cut(s) 401, 560
XmiI GTMKAC 1 cut(s) 174
ZrmI AGTACT 2 cut(s) 722, 770
Zsp2I ATGCAT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.