Rh2CG547000

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0013512
Physical Location & Seq
Forward (+)
72546294 .. 72549367
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG547000.1

Sequence Viewer

Length: 1659 bp
ATGTTTGACATATTGTTCGGGTGGAGAAAAGCTTCAAAGTGCAAGCAGCTGATTAAACAGGTGCAGTGCCGGCTCAAGTTGCTGAAGAACAAGAGGGCTACTATTGTGAGGCAATTGCGAGAAGACGCGGCGGAGCTGATCAAGAATGGCTACGAAGGCAAAGCCTTCAATAGGGTTGAGCAGATAATCAAAGATGAATGCATAGTGGCAGTGTATGAACTGTTGGATAATTTCTGTGAATTTATCCTCCTCCACCTTTCATATATCCGAAGACACAAGGACTGTCCAAATGACATAAATGAAGCAGTTTCAAGTCTTATATATGCTTCTGCTAGATGTGGAGATCTCCCTGAGCTTAGAACAATCCGAAAGCTCTTCGGAGAACGTTATGGTCAGAAATATGCAATGACTGCTCTTGAACTATTCCCCGGGAACCTTGTGAACTGTCAGGTAATAGAGAAACTGTCCCTAAAGTCAGTAACAGATGATATGAAGCACATATTGGTCAATGAAATAACAAGGAATTACTGCATCAAACCAGAGGTTTTAGCCATCGAATACTATTCCGATTGGCAACAGAAGGTAAAGGAAATTAGTGGACATCAAGCACTGGATTCAAATGTTCGGACTTACTATGAGAGATCTGAAATGCAAATTTGCTCTGTCGAAGGGCTTGAAACAGAAGATGTTCATGATTCCAGTATATCTACTACTACTTCAGTAGCTAAGCTGGCCCCGACTGTATCAGACTCTCCGGTGCATAATGAAATACATGAAGAACAGGACTTTGCTGAAGTGGATTCTCCAAGGGGTGGAGAAATAGTGGTGTACAATGATGACATTGAAGAGGTTCAGTCTTGCACAACAAGAGATGGAGATGACTGCCAGGACCAAAGAGCTTTCAAGTTCAAAGCTAGTGTTCCGAAAAAGGAGAATCTTGAAAACAGCTTTGATCGTGGTCATATGAACACATGGAGTGTGAATTCAGGGTCAAGAGTCTCTACGAGAAGTTTTGGGGGAAATGGGAAAAGACCAAGGAAGAGATCAGTGTCTCAGGAAAATCAGTTTTCGAAGGATAGTAAATTTCCAAGTCACTATGCTAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTAGTAAATCCATGTTATGTTTGCAATGGGAATGAAAATGTCGAATTTGAAGTTCCAACATTGAAGCAAAATAGAGGGATTTCAAATAGGGTGGGATTTGTTAATCCTGGTCATGAACATAAAAGTTTGGTGTACAATGTTTTCACTTACCCTGATCACCAGCCAAAACCGCAAAGCAAAGTGTTCAAAGGAAACATAGAGGAATCTGGTTTTCAATGCAAGCTTCCCTCTTATACTTCGTCTTCATCATCATCATCTTCTCCAACTGTGACTAGTTCTTGGACGAGAAAAGAAATGGTACCTCCTTACTTCAGAAATGTGACAATGCCGCCGGAAAGAGTCAACGACAAGCGGAAAGAAGAGTTTCAACGATCCATTTCGGACTGTTTTCAGTTTCCTACTCATGTTCATCCAAAGCTGCCAGATTATGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAAACTAAGCCTCCTCTCAGCAATCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

552

Amino Acids

63.2

Weight (kDa)

8.5

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 178 1.3e-49 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1450
AccB1I GGYRCC 1 cut(s) 1450
AccB7I CCANNNNNTGG 1 cut(s) 812
AccII CGCG 1 cut(s) 128
AciI CCGC 5 cut(s) 128, 131, 1322, 1481, 1504
AclI AACGTT 1 cut(s) 385
AclWI GGATC 1 cut(s) 1518
AcsI RAATTY 6 cut(s) 239, 654, 982, 1082, 1196, 1598
AcuI CTGAAG 4 cut(s) 104, 702, 813, 1447
AfaI GTAC 3 cut(s) 830, 1286, 1452
AfiI CCNNNNNNNGG 2 cut(s) 171, 812
AhlI ACTAGT 1 cut(s) 1424
AjnI CCWGG 2 cut(s) 885, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
Alw26I GTCTC 2 cut(s) 1003, 1056
AlwI GGATC 1 cut(s) 1518
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 1 cut(s) 732
ApeKI GCWGC 4 cut(s) 46, 1149, 1570, 1592
ApoI RAATTY 6 cut(s) 239, 654, 982, 1082, 1196, 1598
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 4 cut(s) 31, 687, 849, 1515
Asp718I GGTACC 1 cut(s) 1450
AspS9I GGNCC 3 cut(s) 733, 889, 1124
AsuC2I CCSGG 2 cut(s) 429, 430
AsuHPI GGTGA 1 cut(s) 1301
AsuII TTCGAA 1 cut(s) 1070
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 889, 1124
BanI GGYRCC 1 cut(s) 1450
BbsI GAAGAC 3 cut(s) 129, 277, 1386
BbvI GCAGC 4 cut(s) 58, 1161, 1557, 1604
BccI CCATC 2 cut(s) 560, 866
BciT130I CCWGG 2 cut(s) 887, 1260
BclI TGATCA 2 cut(s) 138, 1306
BcnI CCSGG 2 cut(s) 429, 430
BcoDI GTCTC 2 cut(s) 1003, 1056
BcuI ACTAGT 1 cut(s) 1424
BfaI CTAG 5 cut(s) 333, 915, 1101, 1154, 1425
BglII AGATCT 2 cut(s) 343, 641
BisI GCNGC 6 cut(s) 47, 129, 1150, 1481, 1571, 1593
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 6 cut(s) 48, 130, 1151, 1482, 1572, 1594
Bme1390I CCNGG 4 cut(s) 429, 430, 887, 1260
Bme18I GGWCC 2 cut(s) 889, 1124
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 733, 889, 1124
BmiI GGNNCC 4 cut(s) 434, 735, 1125, 1452
BmrFI CCNGG 4 cut(s) 429, 430, 887, 1260
BmsI GCATC 2 cut(s) 540, 1117
BpiI GAAGAC 3 cut(s) 129, 277, 1386
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 1070
BpuEI CTTGAG 2 cut(s) 59, 1595
BpuMI CCSGG 2 cut(s) 429, 430
BsaJI CCNNGG 4 cut(s) 427, 428, 806, 1034
BsaWI WCCGGW 1 cut(s) 754
BsaXI ACNNNNNCTCC 4 cut(s) 807, 837, 1621, 1651
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 812
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 615, 699
Bse3DI GCAATG 2 cut(s) 411, 1183
BseBI CCWGG 2 cut(s) 887, 1260
BseDI CCNNGG 4 cut(s) 427, 428, 806, 1034
BseGI GGATG 1 cut(s) 1561
BseLI CCNNNNNNNGG 2 cut(s) 171, 812
BseMI GCAATG 2 cut(s) 411, 1183
BseMII CTCAG 3 cut(s) 342, 1067, 1657
BseNI ACTGG 2 cut(s) 615, 699
BseRI GAGGAG 2 cut(s) 239, 1629
BseXI GCAGC 4 cut(s) 58, 1161, 1557, 1604
BsgI GTGCAG 2 cut(s) 83, 1168
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 734
BshNI GGYRCC 1 cut(s) 1450
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 4 cut(s) 70, 429, 755, 1484
BslFI GGGAC 2 cut(s) 451, 1137
BslI CCNNNNNNNGG 2 cut(s) 171, 812
BsmAI GTCTC 2 cut(s) 1003, 1056
BsmFI GGGAC 2 cut(s) 451, 1137
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 734
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 1 cut(s) 1070
Bsp1407I TGTACA 2 cut(s) 828, 1284
Bsp143I GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
Bsp1720I GCTNAGC 1 cut(s) 726
BspACI CCGC 5 cut(s) 128, 131, 1322, 1481, 1504
BspANI GGCC 1 cut(s) 734
BspCNI CTCAG 3 cut(s) 343, 1066, 1656
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 2 cut(s) 691, 1264
BspLI GGNNCC 4 cut(s) 434, 735, 1125, 1452
BspPI GGATC 1 cut(s) 1518
BspQI GCTCTTC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 1070
BspT107I GGYRCC 1 cut(s) 1450
BsrDI GCAATG 2 cut(s) 411, 1183
BsrFI RCCGGY 1 cut(s) 69
BsrGI TGTACA 2 cut(s) 828, 1284
BsrI ACTGG 2 cut(s) 615, 699
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 4 cut(s) 427, 428, 806, 1034
BssMI GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
BssT1I CCWWGG 2 cut(s) 806, 1034
Bst2UI CCWGG 2 cut(s) 887, 1260
Bst4CI ACNGT 7 cut(s) 222, 284, 446, 465, 742, 1420, 1538
Bst6I CTCTTC 4 cut(s) 380, 840, 1034, 1506
BstAPI GCANNNNNTGC 1 cut(s) 410
BstAUI TGTACA 2 cut(s) 828, 1284
BstBI TTCGAA 1 cut(s) 1070
BstC8I GCNNGC 4 cut(s) 44, 71, 732, 1373
BstDEI CTNAG 6 cut(s) 351, 356, 726, 1053, 1632, 1643
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 1561
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 7 cut(s) 141, 346, 644, 955, 1046, 1309, 1526
BstMAI GTCTC 2 cut(s) 1003, 1056
BstMBI GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
BstMWI GCNNNNNNNGC 8 cut(s) 70, 79, 156, 410, 731, 1149, 1321, 1633
BstNI CCWGG 2 cut(s) 887, 1260
BstSCI CCNGG 4 cut(s) 427, 428, 885, 1258
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 58, 1161, 1557, 1604
BstV2I GAAGAC 3 cut(s) 129, 277, 1386
BstX2I RGATCY 2 cut(s) 343, 641
BstYI RGATCY 2 cut(s) 343, 641
BsuRI GGCC 1 cut(s) 734
BtsCI GGATG 1 cut(s) 1561
BtsI GCAGTG 2 cut(s) 71, 216
BtsIMutI CAGTG 4 cut(s) 71, 216, 608, 1053
Cac8I GCNNGC 4 cut(s) 44, 71, 732, 1373
CciI TCATGA 2 cut(s) 691, 1264
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 733, 889, 1124
Cfr9I CCCGGG 1 cut(s) 428
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 829, 1285, 1451
CspCI CAANNNNNGTGG 2 cut(s) 1224, 1259
CviAII CATG 8 cut(s) 692, 773, 972, 1140, 1164, 1265, 1556, 1603
CviQI GTAC 3 cut(s) 829, 1285, 1451
DdeI CTNAG 6 cut(s) 351, 356, 726, 1053, 1632, 1643
DpnI GATC 7 cut(s) 140, 345, 643, 954, 1045, 1308, 1525
DpnII GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
Eam1104I CTCTTC 4 cut(s) 380, 840, 1034, 1506
EarI CTCTTC 4 cut(s) 380, 840, 1034, 1506
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 806, 1034
Eco47I GGWCC 2 cut(s) 889, 1124
Eco57I CTGAAG 4 cut(s) 104, 702, 813, 1447
Eco88I CYCGRG 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 1124
EcoRI GAATTC 1 cut(s) 982
EcoRII CCWGG 2 cut(s) 885, 1258
EcoT14I CCWWGG 2 cut(s) 806, 1034
EcoT22I ATGCAT 1 cut(s) 203
ErhI CCWWGG 2 cut(s) 806, 1034
FaeI CATG 8 cut(s) 695, 776, 975, 1143, 1167, 1268, 1559, 1606
FaqI GGGAC 2 cut(s) 451, 1137
FatI CATG 8 cut(s) 691, 772, 971, 1139, 1163, 1264, 1555, 1602
FauNDI CATATG 1 cut(s) 963
FbaI TGATCA 2 cut(s) 138, 1306
Fnu4HI GCNGC 6 cut(s) 47, 129, 1150, 1481, 1571, 1593
FokI GGATG 1 cut(s) 1548
Fsp4HI GCNGC 6 cut(s) 47, 129, 1150, 1481, 1571, 1593
FspBI CTAG 5 cut(s) 333, 915, 1101, 1154, 1425
GluI GCNGC 6 cut(s) 47, 129, 1150, 1481, 1571, 1593
HaeIII GGCC 1 cut(s) 734
HapII CCGG 4 cut(s) 70, 429, 755, 1484
HgaI GACGC 1 cut(s) 134
Hin1II CATG 8 cut(s) 695, 776, 975, 1143, 1167, 1268, 1559, 1606
HincII GTYRAC 1 cut(s) 1495
HindII GTYRAC 1 cut(s) 1495
HindIII AAGCTT 2 cut(s) 30, 1373
HinfI GANTC 8 cut(s) 614, 695, 749, 800, 934, 996, 1355, 1491
HpaII CCGG 4 cut(s) 70, 429, 755, 1484
HphI GGTGA 1 cut(s) 1301
Hpy166II GTNNAC 5 cut(s) 442, 599, 829, 1285, 1495
Hpy188III TCNNGA 8 cut(s) 142, 416, 692, 938, 993, 1055, 1265, 1612
Hpy8I GTNNAC 5 cut(s) 442, 599, 829, 1285, 1495
HpyAV CCTTC 5 cut(s) 149, 175, 574, 662, 1066
HpyCH4III ACNGT 7 cut(s) 222, 284, 446, 465, 742, 1420, 1538
HpyCH4IV ACGT 2 cut(s) 385, 1621
HpyF10VI GCNNNNNNNGC 8 cut(s) 70, 79, 156, 410, 731, 1149, 1321, 1633
HpyF3I CTNAG 6 cut(s) 351, 356, 726, 1053, 1632, 1643
HpySE526I ACGT 2 cut(s) 385, 1621
Hsp92II CATG 8 cut(s) 695, 776, 975, 1143, 1167, 1268, 1559, 1606
KpnI GGTACC 1 cut(s) 1454
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 2 cut(s) 138, 1306
Kzo9I GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 58, 1161, 1557, 1604
LweI GCATC 2 cut(s) 540, 1117
MaeI CTAG 5 cut(s) 333, 915, 1101, 1154, 1425
MaeII ACGT 2 cut(s) 385, 1621
MaeIII GTNAC 4 cut(s) 478, 1091, 1420, 1471
MalI GATC 7 cut(s) 140, 345, 643, 954, 1045, 1308, 1525
MboI GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
MfeI CAATTG 1 cut(s) 113
MflI RGATCY 2 cut(s) 343, 641
MlyI GAGTC 3 cut(s) 743, 1005, 1500
MmeI TCCRAC 3 cut(s) 204, 1232, 1439
Mph1103I ATGCAT 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 4 cut(s) 31, 687, 849, 1515
MseI TTAA 2 cut(s) 54, 1254
MslI CAYNNNNRTG 1 cut(s) 1144
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 4 cut(s) 70, 429, 755, 1484
MspR9I CCNGG 4 cut(s) 429, 430, 887, 1260
MunI CAATTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 203
MvaI CCWGG 2 cut(s) 887, 1260
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 8 cut(s) 70, 79, 156, 410, 731, 1149, 1321, 1633
NaeI GCCGGC 1 cut(s) 71
NciI CCSGG 2 cut(s) 429, 430
NdeI CATATG 1 cut(s) 963
NdeII GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 8 cut(s) 695, 776, 975, 1143, 1167, 1268, 1559, 1606
NlaIV GGNNCC 4 cut(s) 434, 735, 1125, 1452
NmuCI GTSAC 3 cut(s) 1091, 1420, 1471
NsiI ATGCAT 1 cut(s) 203
NspV TTCGAA 1 cut(s) 1070
PagI TCATGA 2 cut(s) 691, 1264
PciSI GCTCTTC 1 cut(s) 380
PctI GAATGC 1 cut(s) 203
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 4 cut(s) 31, 687, 849, 1515
PfeI GAWTC 5 cut(s) 614, 695, 800, 934, 1355
PflMI CCANNNNNTGG 1 cut(s) 812
PkrI GCNGC 6 cut(s) 48, 130, 1151, 1482, 1572, 1594
PleI GAGTC 3 cut(s) 743, 1004, 1499
PpsI GAGTC 3 cut(s) 743, 1004, 1499
PpuMI RGGWCCY 1 cut(s) 1124
Psp1406I AACGTT 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 1124
Psp6I CCWGG 2 cut(s) 885, 1258
PspGI CCWGG 2 cut(s) 885, 1258
PspN4I GGNNCC 4 cut(s) 434, 735, 1125, 1452
PspPI GGNCC 3 cut(s) 733, 889, 1124
PspPPI RGGWCCY 1 cut(s) 1124
PsuI RGATCY 2 cut(s) 343, 641
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 3 cut(s) 830, 1286, 1452
RsaNI GTAC 3 cut(s) 829, 1285, 1451
RseI CAYNNNNRTG 1 cut(s) 1144
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 2 cut(s) 54, 1254
SatI GCNGC 6 cut(s) 47, 129, 1150, 1481, 1571, 1593
Sau3AI GATC 7 cut(s) 138, 343, 641, 952, 1043, 1306, 1523
Sau96I GGNCC 3 cut(s) 733, 889, 1124
SchI GAGTC 3 cut(s) 743, 1005, 1500
ScrFI CCNGG 4 cut(s) 429, 430, 887, 1260
SfaNI GCATC 2 cut(s) 540, 1117
SfuI TTCGAA 1 cut(s) 1070
SinI GGWCC 2 cut(s) 889, 1124
SmaI CCCGGG 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 1144
SmlI CTYRAG 2 cut(s) 74, 1610
SmoI CTYRAG 2 cut(s) 74, 1610
SpeI ACTAGT 1 cut(s) 1424
SsiI CCGC 5 cut(s) 128, 131, 1322, 1481, 1504
SspMI CTAG 5 cut(s) 333, 915, 1101, 1154, 1425
StyD4I CCNGG 4 cut(s) 427, 428, 885, 1258
StyI CCWWGG 2 cut(s) 806, 1034
TaaI ACNGT 7 cut(s) 222, 284, 446, 465, 742, 1420, 1538
TaiI ACGT 2 cut(s) 388, 1624
TaqI TCGA 4 cut(s) 555, 666, 1070, 1194
TatI WGTACW 2 cut(s) 828, 1284
TauI GCSGC 2 cut(s) 131, 1483
TfiI GAWTC 5 cut(s) 614, 695, 800, 934, 1355
Tru1I TTAA 2 cut(s) 54, 1254
Tru9I TTAA 2 cut(s) 54, 1254
TscAI CASTG 4 cut(s) 71, 216, 615, 1053
TseFI GTSAC 3 cut(s) 1091, 1420, 1471
TseI GCWGC 4 cut(s) 46, 1149, 1570, 1592
Tsp45I GTSAC 3 cut(s) 1091, 1420, 1471
TspMI CCCGGG 1 cut(s) 428
TspRI CASTG 4 cut(s) 71, 216, 615, 1053
Van91I CCANNNNNTGG 1 cut(s) 812
VpaK11BI GGWCC 2 cut(s) 889, 1124
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 6 cut(s) 239, 654, 982, 1082, 1196, 1598
XmaI CCCGGG 1 cut(s) 428
XmnI GAANNNNTTC 4 cut(s) 31, 687, 849, 1515
XspI CTAG 5 cut(s) 333, 915, 1101, 1154, 1425
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.