Rh2DG586900

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013512
Physical Location & Seq
Forward (+)
81908728 .. 81911846
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG586900.1

Sequence Viewer

Length: 1014 bp
ATGCAAATTTGCTCTGTCGAAGGGCTTGAAACAGAAGATGTTCATGATTCCAGTATATCTACTACTACTTCTTTAGTAGCTAAGCTGGCCCCGACTGTATCAGACTCTCCGGTGTATAATGAAATACAGGAAGAACGGGACTTTGCTGAAGTGGATTCTCCAAGGGGTGGAGAAATAGTGGTGTACAATGATGACATTGAAGAGGTTCAGTCTTGCACAACTAGAGATGGAGATGACTGCCAGGACCAAAGAGCTTTCAAGTTCAAAGCTAGTGTTCCGAAAAAGGAGAATCTTGAAAACAGCTTTGATCGTGGTCATATGAACACATGGAGTGTGAATTCAGGGTCAAGAGTCTCTACGAGAAGTTTTGGGGGAAATGGGAAAAGACCAAGGAAGAGATCAGTGTCTCAGGAAAATCAGTTTTCGAAGGATAGTGAATTTCCAAGTTACTATGCTAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTAGTAAATCCATGTTATGTTTGCAATGGGAATGAAAATGTCGAATTTGAAGTTCCAACATTGAAGCAAAATAGAGGGATTTCAACTAGGGTGGGATTTGCTAATCCTGGTCATGAACATAAAAGGTTGGTGTACAATGTGTTCACTTACCCTGATCACCAGCCAAAACCGCAAAGCAAAGTGTTCAAAGGAAACATAGAGGAATCTGGTTTTCAATGCAAGCTTCCCTCTTATACTTCGTCTTCATCATCATCATCTTCTCCAACTGTGACTAGTTCTTGGACGAGAAAAGAAATGGTACCTCCTTACTTCAGAAATGTGACAATGCCGCCGGAAAGAGTCAACGACAAGCGGAAAGAAGAGTTTCAACGATCCATTTCAGACTGTTTTCAGTTTCCTACTCATGTTCATCCAAAGCTGCCAGATTATGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAAACTAAGCCTCCTCTCAGCAATCAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

337

Amino Acids

38.17

Weight (kDa)

8.0

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 805
AccB1I GGYRCC 1 cut(s) 805
AccB7I CCANNNNNTGG 1 cut(s) 167
AciI CCGC 3 cut(s) 677, 836, 859
AclWI GGATC 1 cut(s) 873
AcsI RAATTY 5 cut(s) 6, 337, 437, 551, 953
AcuI CTGAAG 2 cut(s) 168, 802
AfaI GTAC 3 cut(s) 185, 641, 807
AfiI CCNNNNNNNGG 1 cut(s) 167
AhlI ACTAGT 1 cut(s) 779
AjnI CCWGG 2 cut(s) 240, 613
AluBI AGCT 9 cut(s) 80, 85, 254, 269, 303, 730, 925, 950, 1009
AluI AGCT 9 cut(s) 80, 85, 254, 269, 303, 730, 925, 950, 1009
Alw26I GTCTC 2 cut(s) 358, 411
AlwI GGATC 1 cut(s) 873
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 3 cut(s) 504, 925, 947
ApoI RAATTY 5 cut(s) 6, 337, 437, 551, 953
Asp700I GAANNNNTTC 3 cut(s) 39, 204, 870
Asp718I GGTACC 1 cut(s) 805
AspS9I GGNCC 3 cut(s) 88, 244, 479
AsuHPI GGTGA 1 cut(s) 656
AsuII TTCGAA 1 cut(s) 425
AvaII GGWCC 2 cut(s) 244, 479
BanI GGYRCC 1 cut(s) 805
BbsI GAAGAC 1 cut(s) 741
BbvI GCAGC 3 cut(s) 516, 912, 959
BccI CCATC 1 cut(s) 221
BciT130I CCWGG 2 cut(s) 242, 615
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 358, 411
BcuI ACTAGT 1 cut(s) 779
BfaI CTAG 6 cut(s) 222, 270, 456, 509, 594, 780
BisI GCNGC 4 cut(s) 505, 836, 926, 948
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 4 cut(s) 506, 837, 927, 949
Bme1390I CCNGG 2 cut(s) 242, 615
Bme18I GGWCC 2 cut(s) 244, 479
BmgT120I GGNCC 3 cut(s) 88, 244, 479
BmiI GGNNCC 3 cut(s) 90, 480, 807
BmrFI CCNGG 2 cut(s) 242, 615
BmsI GCATC 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 741
Bpu1102I GCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 950
BsaJI CCNNGG 2 cut(s) 161, 389
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 4 cut(s) 162, 192, 976, 1006
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 51
Bse3DI GCAATG 1 cut(s) 538
BseBI CCWGG 2 cut(s) 242, 615
BseDI CCNNGG 2 cut(s) 161, 389
BseGI GGATG 1 cut(s) 916
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 2 cut(s) 422, 1012
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 1 cut(s) 984
BseXI GCAGC 3 cut(s) 516, 912, 959
BsgI GTGCAG 1 cut(s) 523
BshFI GGCC 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 805
BsiSI CCGG 2 cut(s) 110, 839
BslFI GGGAC 2 cut(s) 152, 492
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 2 cut(s) 358, 411
BsmFI GGGAC 2 cut(s) 152, 492
BsnI GGCC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 425
Bsp1407I TGTACA 2 cut(s) 183, 639
Bsp143I GATC 4 cut(s) 307, 398, 661, 878
Bsp1720I GCTNAGC 1 cut(s) 81
BspACI CCGC 3 cut(s) 677, 836, 859
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 2 cut(s) 421, 1011
BspHI TCATGA 2 cut(s) 43, 619
BspLI GGNNCC 3 cut(s) 90, 480, 807
BspPI GGATC 1 cut(s) 873
BspT104I TTCGAA 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 538
BsrGI TGTACA 2 cut(s) 183, 639
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 161, 389
BssMI GATC 4 cut(s) 307, 398, 661, 878
BssT1I CCWWGG 2 cut(s) 161, 389
Bst2UI CCWGG 2 cut(s) 242, 615
Bst4CI ACNGT 3 cut(s) 97, 775, 893
Bst6I CTCTTC 3 cut(s) 195, 389, 861
BstAUI TGTACA 2 cut(s) 183, 639
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 87, 728
BstDEI CTNAG 4 cut(s) 81, 408, 987, 998
BstF5I GGATG 1 cut(s) 916
BstKTI GATC 4 cut(s) 310, 401, 664, 881
BstMAI GTCTC 2 cut(s) 358, 411
BstMBI GATC 4 cut(s) 307, 398, 661, 878
BstMWI GCNNNNNNNGC 4 cut(s) 86, 504, 676, 988
BstNI CCWGG 2 cut(s) 242, 615
BstSCI CCNGG 2 cut(s) 240, 613
BstV1I GCAGC 3 cut(s) 516, 912, 959
BstV2I GAAGAC 1 cut(s) 741
BsuRI GGCC 1 cut(s) 89
BtsCI GGATG 1 cut(s) 916
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 2 cut(s) 87, 728
CciI TCATGA 2 cut(s) 43, 619
Cfr13I GGNCC 3 cut(s) 88, 244, 479
Csp6I GTAC 3 cut(s) 184, 640, 806
CspCI CAANNNNNGTGG 2 cut(s) 579, 614
CviAII CATG 7 cut(s) 44, 327, 495, 519, 620, 911, 958
CviQI GTAC 3 cut(s) 184, 640, 806
DdeI CTNAG 4 cut(s) 81, 408, 987, 998
DpnI GATC 4 cut(s) 309, 400, 663, 880
DpnII GATC 4 cut(s) 307, 398, 661, 878
Eam1104I CTCTTC 3 cut(s) 195, 389, 861
EarI CTCTTC 3 cut(s) 195, 389, 861
Eco130I CCWWGG 2 cut(s) 161, 389
Eco47I GGWCC 2 cut(s) 244, 479
Eco57I CTGAAG 2 cut(s) 168, 802
EcoO109I RGGNCCY 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 2 cut(s) 240, 613
EcoT14I CCWWGG 2 cut(s) 161, 389
ErhI CCWWGG 2 cut(s) 161, 389
FaeI CATG 7 cut(s) 47, 330, 498, 522, 623, 914, 961
FaqI GGGAC 2 cut(s) 152, 492
FatI CATG 7 cut(s) 43, 326, 494, 518, 619, 910, 957
FauNDI CATATG 1 cut(s) 318
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 4 cut(s) 505, 836, 926, 948
FokI GGATG 1 cut(s) 903
Fsp4HI GCNGC 4 cut(s) 505, 836, 926, 948
FspBI CTAG 6 cut(s) 222, 270, 456, 509, 594, 780
GluI GCNGC 4 cut(s) 505, 836, 926, 948
HaeIII GGCC 1 cut(s) 89
HapII CCGG 2 cut(s) 110, 839
Hin1II CATG 7 cut(s) 47, 330, 498, 522, 623, 914, 961
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 7 cut(s) 47, 104, 155, 289, 351, 710, 846
HpaII CCGG 2 cut(s) 110, 839
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 4 cut(s) 184, 640, 651, 850
Hpy188I TCNGA 5 cut(s) 103, 279, 468, 821, 889
Hpy188III TCNNGA 6 cut(s) 44, 293, 348, 410, 620, 967
Hpy8I GTNNAC 4 cut(s) 184, 640, 651, 850
HpyAV CCTTC 2 cut(s) 14, 421
HpyCH4III ACNGT 3 cut(s) 97, 775, 893
HpyCH4IV ACGT 1 cut(s) 976
HpyCH4V TGCA 8 cut(s) 4, 216, 498, 504, 531, 726, 947, 982
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 504, 676, 988
HpyF3I CTNAG 4 cut(s) 81, 408, 987, 998
HpySE526I ACGT 1 cut(s) 976
Hsp92II CATG 7 cut(s) 47, 330, 498, 522, 623, 914, 961
KpnI GGTACC 1 cut(s) 809
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 4 cut(s) 307, 398, 661, 878
Lsp1109I GCAGC 3 cut(s) 516, 912, 959
LweI GCATC 1 cut(s) 472
MaeI CTAG 6 cut(s) 222, 270, 456, 509, 594, 780
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 3 cut(s) 446, 775, 826
MalI GATC 4 cut(s) 309, 400, 663, 880
MboI GATC 4 cut(s) 307, 398, 661, 878
MboII GAAGA 8 cut(s) 47, 143, 212, 406, 481, 741, 756, 878
MluCI AATT 6 cut(s) 6, 337, 437, 473, 551, 953
MlyI GAGTC 3 cut(s) 98, 360, 855
MmeI TCCRAC 2 cut(s) 587, 794
MnlI CCTC 7 cut(s) 196, 575, 700, 745, 819, 1002, 1005
MroXI GAANNNNTTC 3 cut(s) 39, 204, 870
MslI CAYNNNNRTG 1 cut(s) 499
MspI CCGG 2 cut(s) 110, 839
MspR9I CCNGG 2 cut(s) 242, 615
MvaI CCWGG 2 cut(s) 242, 615
MwoI GCNNNNNNNGC 4 cut(s) 86, 504, 676, 988
NdeI CATATG 1 cut(s) 318
NdeII GATC 4 cut(s) 307, 398, 661, 878
NlaIII CATG 7 cut(s) 47, 330, 498, 522, 623, 914, 961
NlaIV GGNNCC 3 cut(s) 90, 480, 807
NmuCI GTSAC 2 cut(s) 775, 826
NspV TTCGAA 1 cut(s) 425
PagI TCATGA 2 cut(s) 43, 619
PdmI GAANNNNTTC 3 cut(s) 39, 204, 870
PfeI GAWTC 4 cut(s) 47, 155, 289, 710
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 4 cut(s) 506, 837, 927, 949
PleI GAGTC 3 cut(s) 98, 359, 854
PpsI GAGTC 3 cut(s) 98, 359, 854
PpuMI RGGWCCY 1 cut(s) 479
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 2 cut(s) 240, 613
PspGI CCWGG 2 cut(s) 240, 613
PspN4I GGNNCC 3 cut(s) 90, 480, 807
PspPI GGNCC 3 cut(s) 88, 244, 479
PspPPI RGGWCCY 1 cut(s) 479
PsrI GAACNNNNNNTAC 2 cut(s) 632, 664
RsaI GTAC 3 cut(s) 185, 641, 807
RsaNI GTAC 3 cut(s) 184, 640, 806
RseI CAYNNNNRTG 1 cut(s) 499
SatI GCNGC 4 cut(s) 505, 836, 926, 948
Sau3AI GATC 4 cut(s) 307, 398, 661, 878
Sau96I GGNCC 3 cut(s) 88, 244, 479
SchI GAGTC 3 cut(s) 98, 360, 855
ScrFI CCNGG 2 cut(s) 242, 615
SfaNI GCATC 1 cut(s) 472
SfuI TTCGAA 1 cut(s) 425
SinI GGWCC 2 cut(s) 244, 479
SmiMI CAYNNNNRTG 1 cut(s) 499
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
SpeI ACTAGT 1 cut(s) 779
Sse9I AATT 6 cut(s) 6, 337, 437, 473, 551, 953
SsiI CCGC 3 cut(s) 677, 836, 859
SspMI CTAG 6 cut(s) 222, 270, 456, 509, 594, 780
StyD4I CCNGG 2 cut(s) 240, 613
StyI CCWWGG 2 cut(s) 161, 389
TaaI ACNGT 3 cut(s) 97, 775, 893
TaiI ACGT 1 cut(s) 979
TaqI TCGA 3 cut(s) 18, 425, 549
TasI AATT 6 cut(s) 6, 337, 437, 473, 551, 953
TatI WGTACW 2 cut(s) 183, 639
TauI GCSGC 1 cut(s) 838
TfiI GAWTC 4 cut(s) 47, 155, 289, 710
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 2 cut(s) 775, 826
TseI GCWGC 3 cut(s) 504, 925, 947
Tsp45I GTSAC 2 cut(s) 775, 826
TspDTI ATGAA 8 cut(s) 32, 135, 335, 555, 636, 741, 905, 946
TspRI CASTG 1 cut(s) 408
Van91I CCANNNNNTGG 1 cut(s) 167
VpaK11BI GGWCC 2 cut(s) 244, 479
XapI RAATTY 5 cut(s) 6, 337, 437, 551, 953
XmnI GAANNNNTTC 3 cut(s) 39, 204, 870
XspI CTAG 6 cut(s) 222, 270, 456, 509, 594, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.