RLG00000021352

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013512
Physical Location & Seq
Forward (+)
74392479 .. 74394877
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021352

Sequence Viewer

Length: 1665 bp
ATGTTTGACATATTGTTCGGGTGGAGAAAAGCTTCAAAGTGCAAGCAGCTGATCAAACAGGTGCAGTGCCGGCTCAAGTTGCTGAAGAACAAGAGGGCTACTATTGTGAGGCAATTGCGAGAAGACGCGGCGGAGCTAATCAAGAATGGCTACGAAGGCAAAGCCTTCAATAGGGTTGAGCAGATAATCAAAGATGAATGCATAGTGGCAGTGTATGAACTGTTGGATAATTTCTGTGAATTTGTCCTCCTTCACCTTTCATATATTCGAAGACACAAGGACTGTCCAAATGACATAAATGAAGCAGTTTCAAGTCTTATATATGCTTCTGCTAGATGTGGAGATCTCCCTGAGCTTAGAACAATCCGAAATCTCTTCGGAGAACGTTATGGTCAGAAATATGCAATGACTGCTCTTGAACTATTCCCCGGGAACCTTGTGAACTGTCAGGTAATAGAGAAACTGTCCCTAAAGTCAGTAACAGATGATATGAAGCACAGATTGGTCAATGAAATAGCAAGGAATTACTGCATCAAACCAGAGGTTTTAGCCATCGAATACTATTCCGAGTGGCAACAGAAGGCAAAGGAAATTAGTGGACATCAAGCACTGGATTCAAATGTTCGGACTTATTACGAAAGATCTGAAATGCAAATTTGCTCTGTCGAAGGGCTTGAAACAGAAGATGTTCATGATTCCAGTATATCTACTACTACTTCTTCAGTAGCTCAGCTGGCCCCGACTATATCAGACTCTCCGGTGCGTAATGAAATACAGGAAGAACAGGACTTTGCCGAAGTGGATTCTCCAAGGGGTGGAGAAATAGTGGTGTACAATAATGACATTGAAGAGGTTCAGTCTTGCACAACAAGAGATGGAGATGACTGCCAGGACCAAAGAGCTTTCAAGTTCAAAGCTAGTGTTCCGAAAAAGGAGAATCTTGAAAACAGCTTTGATCGTGGTCATGTGAACACATGGAGTGTGAATTCAGGGTCAAGAGTCTCTACGAGAAGTTTTGGGGGAAATGGGAAAAGACCAAGGAAGAGATCAGTGTCTCAGGAAAATCAGTTTTCGAAGGATAGTGAATTTCCAAGTTACTATGCTAGGAAGCATCAGAAGAAATTGGGACCTAACATTTTGTCATGCAAGTGCAGCCTGGTAAATCCATGTTATGTTTGCAATGGGAATGAAAATGCCGAATTTGAAGTTCCAACATTGAAGCAAAATAGAGGGATTTCAACTAGGGTGGGATTTGCTAATCCTGGTCATGAACATAAAAGTTTGGTGTACAATGTTTTCACTTACCCTGATCACCAGCCAAAACCGCAAAGCAAAGTGTTCAAAGGAAACATAGAGGAATCTGGTTTTCAACGCAAGCTTCCCTCTTATACTTCGTCTTCATCATCATCATCTTCTCCAACTGTGACTAGTTCTTGGACGAGAAAAGAAATGGCACCTCCTTACTTCAGAAACATGACAATGCCGCTGGAAAGAGTCAAGGACAAGCGGAAAGAAGAGTTTCAACGATCCATTTCGGACTGTTTTCAGTATCCTACTCATGTTCATCCAAAGCTGCCAGATTATGATGATATTGCAGCTAAATTCATGGCTCTCAAGAAAGAACGTCTGCAAACTAATAAGCCTCCTCGCAGCAATCAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

555

Amino Acids

63.47

Weight (kDa)

8.67

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 15 - 178 2.9e-51 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1453
AccB7I CCANNNNNTGG 1 cut(s) 815
AccII CGCG 1 cut(s) 128
AciI CCGC 5 cut(s) 128, 131, 1325, 1484, 1507
AclI AACGTT 1 cut(s) 385
AclWI GGATC 1 cut(s) 1521
AcsI RAATTY 6 cut(s) 239, 654, 985, 1085, 1199, 1601
AcuI CTGAAG 3 cut(s) 104, 705, 1450
AfaI GTAC 2 cut(s) 833, 1289
AfiI CCNNNNNNNGG 2 cut(s) 171, 815
AhlI ACTAGT 1 cut(s) 1427
AjnI CCWGG 3 cut(s) 888, 1155, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
Alw26I GTCTC 2 cut(s) 1006, 1059
AlwI GGATC 1 cut(s) 1521
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 1 cut(s) 735
ApeKI GCWGC 5 cut(s) 46, 1152, 1573, 1595, 1650
ApoI RAATTY 6 cut(s) 239, 654, 985, 1085, 1199, 1601
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 4 cut(s) 31, 687, 852, 1518
AspS9I GGNCC 3 cut(s) 736, 892, 1127
AsuC2I CCSGG 2 cut(s) 429, 430
AsuHPI GGTGA 2 cut(s) 245, 1304
AsuII TTCGAA 2 cut(s) 268, 1073
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 892, 1127
BanI GGYRCC 1 cut(s) 1453
BbsI GAAGAC 3 cut(s) 129, 277, 1389
BbvI GCAGC 4 cut(s) 58, 1164, 1560, 1607
BccI CCATC 2 cut(s) 560, 869
BciT130I CCWGG 3 cut(s) 890, 1157, 1263
BciVI GTATCC 1 cut(s) 1560
BclI TGATCA 2 cut(s) 51, 1309
BcnI CCSGG 2 cut(s) 429, 430
BcoDI GTCTC 2 cut(s) 1006, 1059
BcuI ACTAGT 1 cut(s) 1427
BfaI CTAG 5 cut(s) 333, 918, 1104, 1242, 1428
BfuI GTATCC 1 cut(s) 1560
BglII AGATCT 2 cut(s) 343, 641
BisI GCNGC 7 cut(s) 47, 129, 1153, 1484, 1574, 1596, 1651
BlpI GCTNAGC 1 cut(s) 729
BlsI GCNGC 7 cut(s) 48, 130, 1154, 1485, 1575, 1597, 1652
Bme1390I CCNGG 5 cut(s) 429, 430, 890, 1157, 1263
Bme18I GGWCC 2 cut(s) 892, 1127
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 736, 892, 1127
BmiI GGNNCC 4 cut(s) 434, 738, 1128, 1455
BmrFI CCNGG 5 cut(s) 429, 430, 890, 1157, 1263
BmsI GCATC 2 cut(s) 540, 1120
BpiI GAAGAC 3 cut(s) 129, 277, 1389
Bpu10I CCTNAGC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 729
Bpu14I TTCGAA 2 cut(s) 268, 1073
BpuEI CTTGAG 2 cut(s) 59, 1598
BpuMI CCSGG 2 cut(s) 429, 430
BsaJI CCNNGG 4 cut(s) 427, 428, 809, 1037
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 2 cut(s) 810, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 815
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 615, 699
Bse3DI GCAATG 2 cut(s) 411, 1186
BseBI CCWGG 3 cut(s) 890, 1157, 1263
BseDI CCNNGG 4 cut(s) 427, 428, 809, 1037
BseGI GGATG 1 cut(s) 1564
BseLI CCNNNNNNNGG 2 cut(s) 171, 815
BseMI GCAATG 2 cut(s) 411, 1186
BseMII CTCAG 3 cut(s) 342, 743, 1070
BseNI ACTGG 2 cut(s) 615, 699
BseRI GAGGAG 1 cut(s) 1635
BseXI GCAGC 4 cut(s) 58, 1164, 1560, 1607
BsgI GTGCAG 2 cut(s) 83, 1171
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 737
BshNI GGYRCC 1 cut(s) 1453
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 3 cut(s) 70, 429, 758
BslFI GGGAC 2 cut(s) 451, 1140
BslI CCNNNNNNNGG 2 cut(s) 171, 815
BsmAI GTCTC 2 cut(s) 1006, 1059
BsmFI GGGAC 2 cut(s) 451, 1140
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 1 cut(s) 737
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 2 cut(s) 268, 1073
Bsp1407I TGTACA 2 cut(s) 831, 1287
Bsp143I GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
Bsp1720I GCTNAGC 1 cut(s) 729
BspACI CCGC 5 cut(s) 128, 131, 1325, 1484, 1507
BspANI GGCC 1 cut(s) 737
BspCNI CTCAG 3 cut(s) 343, 742, 1069
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 2 cut(s) 691, 1267
BspLI GGNNCC 4 cut(s) 434, 738, 1128, 1455
BspPI GGATC 1 cut(s) 1521
BspT104I TTCGAA 2 cut(s) 268, 1073
BspT107I GGYRCC 1 cut(s) 1453
BsrDI GCAATG 2 cut(s) 411, 1186
BsrFI RCCGGY 1 cut(s) 69
BsrGI TGTACA 2 cut(s) 831, 1287
BsrI ACTGG 2 cut(s) 615, 699
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 4 cut(s) 427, 428, 809, 1037
BssMI GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
BssT1I CCWWGG 2 cut(s) 809, 1037
Bst2UI CCWGG 3 cut(s) 890, 1157, 1263
Bst4CI ACNGT 6 cut(s) 222, 284, 446, 465, 1423, 1541
Bst6I CTCTTC 4 cut(s) 380, 843, 1037, 1509
BstAPI GCANNNNNTGC 1 cut(s) 410
BstAUI TGTACA 2 cut(s) 831, 1287
BstBI TTCGAA 2 cut(s) 268, 1073
BstC8I GCNNGC 4 cut(s) 44, 71, 735, 1376
BstDEI CTNAG 4 cut(s) 351, 356, 729, 1056
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 1564
BstFNI CGCG 1 cut(s) 128
BstKTI GATC 7 cut(s) 54, 346, 644, 958, 1049, 1312, 1529
BstMAI GTCTC 2 cut(s) 1006, 1059
BstMBI GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
BstMWI GCNNNNNNNGC 7 cut(s) 70, 79, 156, 410, 734, 1152, 1324
BstNI CCWGG 3 cut(s) 890, 1157, 1263
BstSCI CCNGG 5 cut(s) 427, 428, 888, 1155, 1261
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 58, 1164, 1560, 1607
BstV2I GAAGAC 3 cut(s) 129, 277, 1389
BstX2I RGATCY 2 cut(s) 343, 641
BstYI RGATCY 2 cut(s) 343, 641
BsuI GTATCC 1 cut(s) 1560
BsuRI GGCC 1 cut(s) 737
BtsCI GGATG 1 cut(s) 1564
BtsI GCAGTG 2 cut(s) 71, 216
BtsIMutI CAGTG 4 cut(s) 71, 216, 608, 1056
Cac8I GCNNGC 4 cut(s) 44, 71, 735, 1376
CciI TCATGA 2 cut(s) 691, 1267
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 736, 892, 1127
Cfr9I CCCGGG 1 cut(s) 428
CseI GACGC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 832, 1288
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
CviAII CATG 9 cut(s) 692, 965, 975, 1143, 1167, 1268, 1474, 1559, 1606
CviQI GTAC 2 cut(s) 832, 1288
DdeI CTNAG 4 cut(s) 351, 356, 729, 1056
DpnI GATC 7 cut(s) 53, 345, 643, 957, 1048, 1311, 1528
DpnII GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
Eam1104I CTCTTC 4 cut(s) 380, 843, 1037, 1509
EarI CTCTTC 4 cut(s) 380, 843, 1037, 1509
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 809, 1037
Eco47I GGWCC 2 cut(s) 892, 1127
Eco57I CTGAAG 3 cut(s) 104, 705, 1450
Eco88I CYCGRG 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 1127
EcoRI GAATTC 1 cut(s) 985
EcoRII CCWGG 3 cut(s) 888, 1155, 1261
EcoT14I CCWWGG 2 cut(s) 809, 1037
EcoT22I ATGCAT 1 cut(s) 203
ErhI CCWWGG 2 cut(s) 809, 1037
FaeI CATG 9 cut(s) 695, 968, 978, 1146, 1170, 1271, 1477, 1562, 1609
FaqI GGGAC 2 cut(s) 451, 1140
FatI CATG 9 cut(s) 691, 964, 974, 1142, 1166, 1267, 1473, 1558, 1605
FbaI TGATCA 2 cut(s) 51, 1309
Fnu4HI GCNGC 7 cut(s) 47, 129, 1153, 1484, 1574, 1596, 1651
FokI GGATG 1 cut(s) 1551
Fsp4HI GCNGC 7 cut(s) 47, 129, 1153, 1484, 1574, 1596, 1651
FspBI CTAG 5 cut(s) 333, 918, 1104, 1242, 1428
GluI GCNGC 7 cut(s) 47, 129, 1153, 1484, 1574, 1596, 1651
HaeIII GGCC 1 cut(s) 737
HapII CCGG 3 cut(s) 70, 429, 758
HgaI GACGC 1 cut(s) 134
Hin1II CATG 9 cut(s) 695, 968, 978, 1146, 1170, 1271, 1477, 1562, 1609
HindIII AAGCTT 2 cut(s) 30, 1376
HinfI GANTC 8 cut(s) 614, 695, 752, 803, 937, 999, 1358, 1494
HpaII CCGG 3 cut(s) 70, 429, 758
HphI GGTGA 2 cut(s) 245, 1304
Hpy166II GTNNAC 5 cut(s) 442, 599, 832, 970, 1288
Hpy188III TCNNGA 8 cut(s) 142, 416, 692, 941, 996, 1058, 1268, 1615
Hpy8I GTNNAC 5 cut(s) 442, 599, 832, 970, 1288
HpyAV CCTTC 6 cut(s) 149, 175, 260, 574, 662, 1069
HpyCH4III ACNGT 6 cut(s) 222, 284, 446, 465, 1423, 1541
HpyCH4IV ACGT 2 cut(s) 385, 1624
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 79, 156, 410, 734, 1152, 1324
HpyF3I CTNAG 4 cut(s) 351, 356, 729, 1056
HpySE526I ACGT 2 cut(s) 385, 1624
Hsp92II CATG 9 cut(s) 695, 968, 978, 1146, 1170, 1271, 1477, 1562, 1609
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 2 cut(s) 51, 1309
Kzo9I GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 58, 1164, 1560, 1607
LweI GCATC 2 cut(s) 540, 1120
MaeI CTAG 5 cut(s) 333, 918, 1104, 1242, 1428
MaeII ACGT 2 cut(s) 385, 1624
MaeIII GTNAC 3 cut(s) 478, 1094, 1423
MalI GATC 7 cut(s) 53, 345, 643, 957, 1048, 1311, 1528
MboI GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
MfeI CAATTG 1 cut(s) 113
MflI RGATCY 2 cut(s) 343, 641
MlyI GAGTC 3 cut(s) 746, 1008, 1503
MmeI TCCRAC 3 cut(s) 204, 1235, 1442
Mph1103I ATGCAT 1 cut(s) 203
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 4 cut(s) 31, 687, 852, 1518
MseI TTAA 1 cut(s) 1663
MslI CAYNNNNRTG 2 cut(s) 1147, 1478
MspA1I CMGCKG 3 cut(s) 49, 733, 1486
MspI CCGG 3 cut(s) 70, 429, 758
MspR9I CCNGG 5 cut(s) 429, 430, 890, 1157, 1263
MunI CAATTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 203
MvaI CCWGG 3 cut(s) 890, 1157, 1263
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 7 cut(s) 70, 79, 156, 410, 734, 1152, 1324
NaeI GCCGGC 1 cut(s) 71
NciI CCSGG 2 cut(s) 429, 430
NdeII GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 9 cut(s) 695, 968, 978, 1146, 1170, 1271, 1477, 1562, 1609
NlaIV GGNNCC 4 cut(s) 434, 738, 1128, 1455
NmuCI GTSAC 1 cut(s) 1423
NsiI ATGCAT 1 cut(s) 203
NspV TTCGAA 2 cut(s) 268, 1073
PagI TCATGA 2 cut(s) 691, 1267
PctI GAATGC 1 cut(s) 203
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 4 cut(s) 31, 687, 852, 1518
PfeI GAWTC 5 cut(s) 614, 695, 803, 937, 1358
PflMI CCANNNNNTGG 1 cut(s) 815
PkrI GCNGC 7 cut(s) 48, 130, 1154, 1485, 1575, 1597, 1652
PleI GAGTC 3 cut(s) 746, 1007, 1502
PpsI GAGTC 3 cut(s) 746, 1007, 1502
PpuMI RGGWCCY 1 cut(s) 1127
Psp1406I AACGTT 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 1127
Psp6I CCWGG 3 cut(s) 888, 1155, 1261
PspGI CCWGG 3 cut(s) 888, 1155, 1261
PspN4I GGNNCC 4 cut(s) 434, 738, 1128, 1455
PspPI GGNCC 3 cut(s) 736, 892, 1127
PspPPI RGGWCCY 1 cut(s) 1127
PsuI RGATCY 2 cut(s) 343, 641
PvuII CAGCTG 2 cut(s) 49, 733
RsaI GTAC 2 cut(s) 833, 1289
RsaNI GTAC 2 cut(s) 832, 1288
RseI CAYNNNNRTG 2 cut(s) 1147, 1478
SaqAI TTAA 1 cut(s) 1663
SatI GCNGC 7 cut(s) 47, 129, 1153, 1484, 1574, 1596, 1651
Sau3AI GATC 7 cut(s) 51, 343, 641, 955, 1046, 1309, 1526
Sau96I GGNCC 3 cut(s) 736, 892, 1127
SchI GAGTC 3 cut(s) 746, 1008, 1503
ScrFI CCNGG 5 cut(s) 429, 430, 890, 1157, 1263
SfaNI GCATC 2 cut(s) 540, 1120
SfuI TTCGAA 2 cut(s) 268, 1073
SinI GGWCC 2 cut(s) 892, 1127
SmaI CCCGGG 1 cut(s) 430
SmiMI CAYNNNNRTG 2 cut(s) 1147, 1478
SmlI CTYRAG 2 cut(s) 74, 1613
SmoI CTYRAG 2 cut(s) 74, 1613
SpeI ACTAGT 1 cut(s) 1427
SsiI CCGC 5 cut(s) 128, 131, 1325, 1484, 1507
SspMI CTAG 5 cut(s) 333, 918, 1104, 1242, 1428
StyD4I CCNGG 5 cut(s) 427, 428, 888, 1155, 1261
StyI CCWWGG 2 cut(s) 809, 1037
TaaI ACNGT 6 cut(s) 222, 284, 446, 465, 1423, 1541
TaiI ACGT 2 cut(s) 388, 1627
TaqI TCGA 4 cut(s) 268, 555, 666, 1073
TatI WGTACW 2 cut(s) 831, 1287
TauI GCSGC 2 cut(s) 131, 1486
TfiI GAWTC 5 cut(s) 614, 695, 803, 937, 1358
Tru1I TTAA 1 cut(s) 1663
Tru9I TTAA 1 cut(s) 1663
TscAI CASTG 4 cut(s) 71, 216, 615, 1056
TseFI GTSAC 1 cut(s) 1423
TseI GCWGC 5 cut(s) 46, 1152, 1573, 1595, 1650
Tsp45I GTSAC 1 cut(s) 1423
TspMI CCCGGG 1 cut(s) 428
TspRI CASTG 4 cut(s) 71, 216, 615, 1056
Van91I CCANNNNNTGG 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 892, 1127
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 6 cut(s) 239, 654, 985, 1085, 1199, 1601
XmaI CCCGGG 1 cut(s) 428
XmnI GAANNNNTTC 4 cut(s) 31, 687, 852, 1518
XspI CTAG 5 cut(s) 333, 918, 1104, 1242, 1428
Zsp2I ATGCAT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.