MD17G1078700.v1.1

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013512
Physical Location & Seq
Reverse (-)
6512880 .. 6515987
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1078700.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGTCCAGTTGCCTAGTCTCTATTAACGAGGATTTCCGTAGTGGCCTCACAAGTGTCCCGAAAGACTTGCAGTGGGAGCAGTTTCTCAAGGAGACCAACAACTGTCTCCCCAAAATTTGTTCTGCGATTGTAGAAATGTTTGACATCTTATTCGGGTGGCGAAAGGCTTCGAAGTGCAAGAGGCTGATCAAAGAGGTACAGTGCCGGCTCAAGTTGCTAAAAACCAAGAGGCAAACAATTGTGAGGCAACTACGCCAAGATGTTGCGGAGCTGATCAAACATGGTCACGAAGACAAGGCCTTCAACAGGGTTGAGCAGATAATCAAAGACGAATCCGTAGAGGCACTGTATGAATTGTTGGACAATTTCTGTGAATTCATCCTCATCCACTTTCCTTACATCCGCAGACGCAAGGACTGTCCAAATGACATAAATGAGGCGGTCTCAAGTCTTATATATGCTTCTGCAAGATGTGGGGATCTGCCTGAGCTTAGGAGGATCAGGAAGCTATTCGGAGAACGTTACGGGCAGAAATTCAAAATGAGTGCTCTTGAACTACTCCCTGGGAATCTGGTGAACCGTGAGGTAATAGAGAAACTCTCCGAAAAGTTGGTAACAGATGATATGAAGCATATATTGGTCAGTGAAATAGCAAGAAATTACTGCATCAAACCAGAGGTTTTAGCTATTGAGTACTATTCAGAATGGCAACAAAAGGTGAAGGAAATCAGTGGACATCAAGCACTGGATGCATATGTCCAGACTTGTTATGAACAAACCGAAACATCTGAAAGGAAAATTTTAAATGTTGAGGAAATCGAAAGAGAACCCGTAGATGCTGATTCTATTGTATCTAGAACTTCTGCTAGTTCTTCATTAGCTACTCCACTGATTGAAGGGAATTTTTCTGAAGTTGGTTCTCCAAATATATCTACCCCGGGCACTGAAGAAAGTCGGGGCAGTACTTCTGGCAGCACAAGTCATGCTACAACAAGAGATGAAGATATTGTGATTTACCTTGATGACATAGAAGAGGTTCAGTTTTCTACAACAAGAGATGCAGACTCCCAGGACCAAAGACTCTTCAAATTCAAAGCTATAGCACCGAAGAAAGAGACTCCCGTAAGCAGTTGTGATCTCAGCTATGTGGATTGTTATGAGACGTGGAGTGAGAATTCCGAGTCAAAAAGCCCTAGGAGATCAAGTTGGAAGGGAAGTGGGAAAAGACTGAGGAAGAGATCGGTTTCTCAGGAAAATCAATGGTTGAAGGATAATGAATGTCAAAGTTACTATGGCAGAAAGCATCTGAAAAATCAAAGTAGGTGTGGAACTGATACAAGGATATTAAGCTGTGGTTGCGCCCTGGGAAATCCATGTTATTTTTCCTTCCGAAACGACGAAAATATTGATTGTGAAGTTCAACCGTGGAAGCTAAAGAGAGGGATTACAACTAGGGTTGGATTTCCAACTCATGGACATGAACAATTGAACAGGGGAGTCGAATGGCGTGCAGTGCCTCGAGAGCGAATGAGGAGGAAGAACTATGGCAATGAAGCTATGCTGTACAATGTTTTCACATATCCGGATCATCAGCATCCCAATATGCAAAACAAGGTGATCAAAGGAAGAGTGGAGGAATCGCATTTTCGATGCAGGCGTGCCTCATATAGTTCTACATCTCCAAATGTGACTAATTCTTGGAGTGCAAGAAAGGAAACGGTGCCTCCTTATTTCAGAACCGTGACAATGCCGCCTGAAAGAGCCAAGGACAATCACAAAGACGACTTCCAACGATCATATTCCGACGCCTTCCAGTATCCTACTCACGTCCACCCCAAGCTGCCTGACTATGATGACATAGCAGCTAAATTCATGGCACTAAAGAAAGAGCGTCTGAAAAATAAGCCTCATTGCAGCAACCAGAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

644

Amino Acids

74.48

Weight (kDa)

8.63

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 60 - 223 3.3e-51 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1720
AccB7I CCANNNNNTGG 1 cut(s) 1472
AccIII TCCGGA 1 cut(s) 1582
AciI CCGC 4 cut(s) 266, 403, 440, 1751
AclI AACGTT 1 cut(s) 520
AclWI GGATC 3 cut(s) 486, 506, 1593
AcsI RAATTY 9 cut(s) 114, 374, 533, 798, 901, 1088, 1174, 1868, 1926
AcuI CTGAAG 2 cut(s) 930, 966
AcyI GRCGYC 1 cut(s) 1806
AfaI GTAC 4 cut(s) 198, 695, 964, 1565
AfiI CCNNNNNNNGG 2 cut(s) 306, 1472
AgsI TTSAA 9 cut(s) 304, 538, 554, 896, 1087, 1093, 1267, 1421, 1489
AjiI CACGTC 2 cut(s) 1164, 1828
AjnI CCWGG 3 cut(s) 562, 1068, 1362
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 6 cut(s) 22, 86, 110, 448, 1109, 1154
AlwI GGATC 3 cut(s) 486, 506, 1593
Ama87I CYCGRG 2 cut(s) 937, 1518
Aor13HI TCCGGA 1 cut(s) 1582
AoxI GGCC 2 cut(s) 43, 297
ApeKI GCWGC 4 cut(s) 972, 1840, 1862, 1914
ApoI RAATTY 9 cut(s) 114, 374, 533, 798, 901, 1088, 1174, 1868, 1926
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 3 cut(s) 166, 509, 1035
AspA2I CCTAGG 1 cut(s) 1193
AspLEI GCGC 1 cut(s) 1361
AspS9I GGNCC 1 cut(s) 1072
AsuC2I CCSGG 2 cut(s) 938, 939
AsuHPI GGTGA 3 cut(s) 586, 730, 1627
AsuII TTCGAA 1 cut(s) 170
AvaI CYCGRG 2 cut(s) 937, 1518
AvaII GGWCC 1 cut(s) 1072
AvrII CCTAGG 1 cut(s) 1193
BaeGI GKGCMC 1 cut(s) 944
BanI GGYRCC 1 cut(s) 1720
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 4 cut(s) 984, 1827, 1874, 1926
BcgI CGANNNNNNTGC 4 cut(s) 49, 83, 1490, 1524
BciT130I CCWGG 3 cut(s) 564, 1070, 1364
BciVI GTATCC 1 cut(s) 1827
BclI TGATCA 3 cut(s) 186, 273, 1617
BcnI CCSGG 2 cut(s) 938, 939
BcoDI GTCTC 6 cut(s) 22, 86, 110, 448, 1109, 1154
BfaI CTAG 5 cut(s) 14, 855, 867, 1194, 1452
BfmI CTRYAG 1 cut(s) 1098
BfuI GTATCC 1 cut(s) 1827
BisI GCNGC 5 cut(s) 973, 1751, 1841, 1863, 1915
BlnI CCTAGG 1 cut(s) 1193
BlsI GCNGC 5 cut(s) 974, 1752, 1842, 1864, 1916
BmcAI AGTACT 2 cut(s) 695, 964
Bme1390I CCNGG 5 cut(s) 564, 938, 939, 1070, 1364
Bme18I GGWCC 1 cut(s) 1072
BmeT110I CYCGRG 2 cut(s) 937, 1518
BmgBI CACGTC 2 cut(s) 1164, 1828
BmgT120I GGNCC 1 cut(s) 1072
BmiI GGNNCC 1 cut(s) 1722
BmrFI CCNGG 5 cut(s) 564, 938, 939, 1070, 1364
BmsI GCATC 7 cut(s) 675, 739, 826, 1048, 1312, 1603, 1640
BpiI GAAGAC 1 cut(s) 297
BplI GAGNNNNNCTC 4 cut(s) 428, 460, 584, 616
Bpu10I CCTNAGC 2 cut(s) 486, 491
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 3 cut(s) 71, 194, 430
BpuMI CCSGG 2 cut(s) 938, 939
BsaHI GRCGYC 1 cut(s) 1806
BsaI GGTCTC 2 cut(s) 86, 448
BsaWI WCCGGW 1 cut(s) 1582
BsaXI ACNNNNNCTCC 4 cut(s) 1189, 1219, 1708, 1738
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 1472
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 3 cut(s) 6, 750, 1813
Bse3DI GCAATG 2 cut(s) 1555, 1909
BseAI TCCGGA 1 cut(s) 1582
BseBI CCWGG 3 cut(s) 564, 1070, 1364
BseGI GGATG 5 cut(s) 378, 384, 399, 754, 1594
BseLI CCNNNNNNNGG 2 cut(s) 306, 1472
BseMI GCAATG 2 cut(s) 1555, 1909
BseMII CTCAG 4 cut(s) 477, 1153, 1220, 1262
BseNI ACTGG 3 cut(s) 6, 750, 1813
BseRI GAGGAG 1 cut(s) 1546
BseSI GKGCMC 1 cut(s) 944
BseXI GCAGC 4 cut(s) 984, 1827, 1874, 1926
BsgI GTGCAG 1 cut(s) 1530
BshFI GGCC 2 cut(s) 45, 299
BshNI GGYRCC 1 cut(s) 1720
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 2 cut(s) 937, 1518
BsiSI CCGG 3 cut(s) 205, 938, 1583
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 306, 1472
BsmAI GTCTC 6 cut(s) 22, 86, 110, 448, 1109, 1154
BsmBI CGTCTC 1 cut(s) 1154
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 2 cut(s) 45, 299
Bso31I GGTCTC 2 cut(s) 86, 448
BsoBI CYCGRG 2 cut(s) 937, 1518
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 550, 944
Bsp13I TCCGGA 1 cut(s) 1582
Bsp1407I TGTACA 1 cut(s) 1563
BspACI CCGC 4 cut(s) 266, 403, 440, 1751
BspANI GGCC 2 cut(s) 45, 299
BspCNI CTCAG 4 cut(s) 478, 1152, 1221, 1261
BspEI TCCGGA 1 cut(s) 1582
BspLI GGNNCC 1 cut(s) 1722
BspPI GGATC 3 cut(s) 486, 506, 1593
BspT104I TTCGAA 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 1720
BspTNI GGTCTC 2 cut(s) 86, 448
BsrDI GCAATG 2 cut(s) 1555, 1909
BsrFI RCCGGY 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 1563
BsrI ACTGG 3 cut(s) 6, 750, 1813
BssAI RCCGGY 1 cut(s) 204
BssNI GRCGYC 1 cut(s) 1806
BssT1I CCWWGG 2 cut(s) 1193, 1764
Bst2UI CCWGG 3 cut(s) 564, 1070, 1364
Bst4CI ACNGT 8 cut(s) 104, 201, 348, 419, 581, 1425, 1720, 1741
Bst6I CTCTTC 4 cut(s) 1026, 1088, 1229, 1621
BstACI GRCGYC 1 cut(s) 1806
BstAPI GCANNNNNTGC 1 cut(s) 749
BstAUI TGTACA 1 cut(s) 1563
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 4 cut(s) 206, 1509, 1655, 1659
BstDEI CTNAG 5 cut(s) 486, 491, 1139, 1229, 1248
BstDSI CCRYGG 1 cut(s) 1424
BstENI CCTNNNNNAGG 1 cut(s) 304
BstF5I GGATG 5 cut(s) 378, 384, 399, 754, 1594
BstHHI GCGC 1 cut(s) 1361
BstMAI GTCTC 6 cut(s) 22, 86, 110, 448, 1109, 1154
BstMWI GCNNNNNNNGC 6 cut(s) 76, 214, 749, 1356, 1513, 1522
BstNI CCWGG 3 cut(s) 564, 1070, 1364
BstSCI CCNGG 5 cut(s) 562, 936, 937, 1068, 1362
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 1 cut(s) 944
BstV1I GCAGC 4 cut(s) 984, 1827, 1874, 1926
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuI GTATCC 1 cut(s) 1827
BsuRI GGCC 2 cut(s) 45, 299
BtgI CCRYGG 1 cut(s) 1424
BtrI CACGTC 2 cut(s) 1164, 1828
BtsCI GGATG 5 cut(s) 378, 384, 399, 754, 1594
BtsI GCAGTG 2 cut(s) 77, 1518
BtsIMutI CAGTG 9 cut(s) 77, 206, 344, 649, 736, 743, 887, 942, 1518
Cac8I GCNNGC 4 cut(s) 206, 1509, 1655, 1659
CfoI GCGC 1 cut(s) 1361
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 1072
Cfr9I CCCGGG 1 cut(s) 937
CseI GACGC 3 cut(s) 417, 1814, 1880
Csp6I GTAC 4 cut(s) 197, 694, 963, 1564
CviAII CATG 6 cut(s) 281, 983, 1374, 1472, 1478, 1873
CviQI GTAC 4 cut(s) 197, 694, 963, 1564
DdeI CTNAG 5 cut(s) 486, 491, 1139, 1229, 1248
DraI TTTAAA 1 cut(s) 804
Eam1104I CTCTTC 4 cut(s) 1026, 1088, 1229, 1621
EarI CTCTTC 4 cut(s) 1026, 1088, 1229, 1621
Eco130I CCWWGG 2 cut(s) 1193, 1764
Eco147I AGGCCT 1 cut(s) 299
Eco31I GGTCTC 2 cut(s) 86, 448
Eco47I GGWCC 1 cut(s) 1072
Eco57I CTGAAG 2 cut(s) 930, 966
Eco88I CYCGRG 2 cut(s) 937, 1518
EcoNI CCTNNNNNAGG 1 cut(s) 304
EcoRI GAATTC 2 cut(s) 374, 1174
EcoRII CCWGG 3 cut(s) 562, 1068, 1362
EcoT14I CCWWGG 2 cut(s) 1193, 1764
EcoT22I ATGCAT 1 cut(s) 754
ErhI CCWWGG 2 cut(s) 1193, 1764
Esp3I CGTCTC 1 cut(s) 1154
FaeI CATG 6 cut(s) 284, 986, 1377, 1475, 1481, 1876
FaqI GGGAC 1 cut(s) 41
FatI CATG 6 cut(s) 280, 982, 1373, 1471, 1477, 1872
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 3 cut(s) 186, 273, 1617
Fnu4HI GCNGC 5 cut(s) 973, 1751, 1841, 1863, 1915
FokI GGATG 5 cut(s) 365, 371, 386, 761, 1581
Fsp4HI GCNGC 5 cut(s) 973, 1751, 1841, 1863, 1915
FspBI CTAG 5 cut(s) 14, 855, 867, 1194, 1452
GlaI GCGC 1 cut(s) 1360
GluI GCNGC 5 cut(s) 973, 1751, 1841, 1863, 1915
HaeIII GGCC 2 cut(s) 45, 299
HapII CCGG 3 cut(s) 205, 938, 1583
HgaI GACGC 3 cut(s) 417, 1814, 1880
HhaI GCGC 1 cut(s) 1361
Hin1I GRCGYC 1 cut(s) 1806
Hin1II CATG 6 cut(s) 284, 986, 1377, 1475, 1481, 1876
Hin6I GCGC 1 cut(s) 1359
HinP1I GCGC 1 cut(s) 1359
HinfI GANTC 9 cut(s) 332, 568, 842, 1064, 1080, 1117, 1181, 1497, 1637
HpaII CCGG 3 cut(s) 205, 938, 1583
HphI GGTGA 3 cut(s) 586, 730, 1627
Hpy166II GTNNAC 3 cut(s) 577, 734, 1831
Hpy188III TCNNGA 9 cut(s) 58, 287, 502, 551, 760, 855, 1250, 1520, 1583
Hpy8I GTNNAC 3 cut(s) 577, 734, 1831
Hpy99I CGWCG 2 cut(s) 1400, 1808
HpyAV CCTTC 7 cut(s) 310, 715, 890, 1204, 1261, 1396, 1819
HpyCH4III ACNGT 8 cut(s) 104, 201, 348, 419, 581, 1425, 1720, 1741
HpyCH4IV ACGT 3 cut(s) 520, 1163, 1827
HpyF10VI GCNNNNNNNGC 6 cut(s) 76, 214, 749, 1356, 1513, 1522
HpyF3I CTNAG 5 cut(s) 486, 491, 1139, 1229, 1248
HpySE526I ACGT 3 cut(s) 520, 1163, 1827
Hsp92I GRCGYC 1 cut(s) 1806
Hsp92II CATG 6 cut(s) 284, 986, 1377, 1475, 1481, 1876
HspAI GCGC 1 cut(s) 1359
Kpn2I TCCGGA 1 cut(s) 1582
KroI GCCGGC 1 cut(s) 204
KroNI GCCGGC 1 cut(s) 206
Ksp22I TGATCA 3 cut(s) 186, 273, 1617
LmnI GCTCC 2 cut(s) 76, 268
Lsp1109I GCAGC 4 cut(s) 984, 1827, 1874, 1926
LweI GCATC 7 cut(s) 675, 739, 826, 1048, 1312, 1603, 1640
MaeI CTAG 5 cut(s) 14, 855, 867, 1194, 1452
MaeII ACGT 3 cut(s) 520, 1163, 1827
MaeIII GTNAC 6 cut(s) 284, 521, 613, 1286, 1687, 1741
MfeI CAATTG 2 cut(s) 237, 1484
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 2 cut(s) 550, 944
MlyI GAGTC 5 cut(s) 1058, 1074, 1111, 1190, 1506
MmeI TCCRAC 6 cut(s) 339, 1187, 1438, 1490, 1813, 1827
Mph1103I ATGCAT 1 cut(s) 754
MroI TCCGGA 1 cut(s) 1582
MroNI GCCGGC 1 cut(s) 204
MroXI GAANNNNTTC 3 cut(s) 166, 509, 1035
MseI TTAA 3 cut(s) 24, 803, 1346
MslI CAYNNNNRTG 1 cut(s) 1476
MspI CCGG 3 cut(s) 205, 938, 1583
MspR9I CCNGG 5 cut(s) 564, 938, 939, 1070, 1364
MunI CAATTG 2 cut(s) 237, 1484
MvaI CCWGG 3 cut(s) 564, 1070, 1364
MwoI GCNNNNNNNGC 6 cut(s) 76, 214, 749, 1356, 1513, 1522
NaeI GCCGGC 1 cut(s) 206
NciI CCSGG 2 cut(s) 938, 939
NdeI CATATG 1 cut(s) 754
NgoMIV GCCGGC 1 cut(s) 204
NlaIII CATG 6 cut(s) 284, 986, 1377, 1475, 1481, 1876
NlaIV GGNNCC 1 cut(s) 1722
NmuCI GTSAC 3 cut(s) 284, 1687, 1741
NsiI ATGCAT 1 cut(s) 754
NspV TTCGAA 1 cut(s) 170
PaeR7I CTCGAG 1 cut(s) 1518
PasI CCCWGGG 2 cut(s) 563, 1363
PceI AGGCCT 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 1654
PdiI GCCGGC 1 cut(s) 206
PdmI GAANNNNTTC 3 cut(s) 166, 509, 1035
PfeI GAWTC 4 cut(s) 332, 568, 842, 1637
PflMI CCANNNNNTGG 1 cut(s) 1472
PkrI GCNGC 5 cut(s) 974, 1752, 1842, 1864, 1916
PleI GAGTC 5 cut(s) 1058, 1074, 1111, 1189, 1505
PpsI GAGTC 5 cut(s) 1058, 1074, 1111, 1189, 1505
Psp1406I AACGTT 1 cut(s) 520
Psp6I CCWGG 3 cut(s) 562, 1068, 1362
PspGI CCWGG 3 cut(s) 562, 1068, 1362
PspN4I GGNNCC 1 cut(s) 1722
PspPI GGNCC 1 cut(s) 1072
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 4 cut(s) 198, 695, 964, 1565
RsaNI GTAC 4 cut(s) 197, 694, 963, 1564
RseI CAYNNNNRTG 1 cut(s) 1476
SaqAI TTAA 3 cut(s) 24, 803, 1346
SatI GCNGC 5 cut(s) 973, 1751, 1841, 1863, 1915
Sau96I GGNCC 1 cut(s) 1072
ScaI AGTACT 2 cut(s) 695, 964
SchI GAGTC 5 cut(s) 1058, 1074, 1111, 1190, 1506
ScrFI CCNGG 5 cut(s) 564, 938, 939, 1070, 1364
SduI GDGCHC 2 cut(s) 550, 944
SfaNI GCATC 7 cut(s) 675, 739, 826, 1048, 1312, 1603, 1640
SfcI CTRYAG 1 cut(s) 1098
Sfr274I CTCGAG 1 cut(s) 1518
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 1 cut(s) 1072
SlaI CTCGAG 1 cut(s) 1518
SmaI CCCGGG 1 cut(s) 939
SmiMI CAYNNNNRTG 1 cut(s) 1476
SmlI CTYRAG 4 cut(s) 86, 209, 445, 1518
SmoI CTYRAG 4 cut(s) 86, 209, 445, 1518
SseBI AGGCCT 1 cut(s) 299
SsiI CCGC 4 cut(s) 266, 403, 440, 1751
SspI AATATT 1 cut(s) 1405
SspMI CTAG 5 cut(s) 14, 855, 867, 1194, 1452
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 5 cut(s) 562, 936, 937, 1068, 1362
StyI CCWWGG 2 cut(s) 1193, 1764
TaaI ACNGT 8 cut(s) 104, 201, 348, 419, 581, 1425, 1720, 1741
TaiI ACGT 3 cut(s) 523, 1166, 1830
TaqI TCGA 5 cut(s) 170, 819, 1500, 1519, 1648
TatI WGTACW 3 cut(s) 693, 962, 1563
TauI GCSGC 1 cut(s) 1753
TfiI GAWTC 4 cut(s) 332, 568, 842, 1637
Tru1I TTAA 3 cut(s) 24, 803, 1346
Tru9I TTAA 3 cut(s) 24, 803, 1346
TscAI CASTG 9 cut(s) 77, 206, 351, 649, 736, 750, 894, 949, 1518
TseFI GTSAC 3 cut(s) 284, 1687, 1741
TseI GCWGC 4 cut(s) 972, 1840, 1862, 1914
Tsp45I GTSAC 3 cut(s) 284, 1687, 1741
TspGWI ACGGA 2 cut(s) 26, 325
TspMI CCCGGG 1 cut(s) 937
TspRI CASTG 9 cut(s) 77, 206, 351, 649, 736, 750, 894, 949, 1518
Van91I CCANNNNNTGG 1 cut(s) 1472
VpaK11BI GGWCC 1 cut(s) 1072
XagI CCTNNNNNAGG 1 cut(s) 304
XapI RAATTY 9 cut(s) 114, 374, 533, 798, 901, 1088, 1174, 1868, 1926
XbaI TCTAGA 1 cut(s) 854
XhoI CTCGAG 1 cut(s) 1518
XmaI CCCGGG 1 cut(s) 937
XmaJI CCTAGG 1 cut(s) 1193
XmnI GAANNNNTTC 3 cut(s) 166, 509, 1035
XspI CTAG 5 cut(s) 14, 855, 867, 1194, 1452
ZrmI AGTACT 2 cut(s) 695, 964
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.