AT2G15860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008704
Physical Location & Seq
Reverse (-)
6905379 .. 6909498
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G15860.4

Sequence Viewer

Length: 1539 bp
ATGGAAGAAGACAAGCAAATGCACGAGGAGAAATCATCTCTGGTTAAAGAAGAACCGGTTCGTGGAGAAGAGCCCGAGATTGAGAAATTGACAGTGGCAGATGTGGACGCGCCACCGAAGAGTACCGGTGGTTGGGGTTGGGGTTTCTCTGGCTTCTCTGTGCTTTCCGATCTCCAAAAAGCTGCGGAAGATATTTCTCGTAATGCTGCAGCGGTGGCAGAGAAAGCAGCGAAAAGCATTGCAGAGATGGGAGAAGTGGATGAAGACTCTGAATCTTCTGCTAAGGAAGAAGAGAAAACTGAAGAAGCTGATACAGAGCAGGATAGTGATGATGAGAATGCGAAGTTAAAGAAGTCAGCTCTTGAGAGATTGGAGGGTGCAAGTGAAGAGTCACTTCTTAGCCAGGGTTTGAAGGTTTTCGATGATTCAGTTGAGAGTTTCACTTCTGGAGCTTGGCAAGCATTTGGAAATGCGTTAAAAGGGGGCACAAGTTTGGTGCAAAAGCTTGAAAACAGTGTCCAGCAAGGTTCTTCGCCTAGGGAAGCTGGATCTGGTGCACCGTCTCTACTAGAGACAGGAAAAGCATTAACTGCGAAAGGAATGCAAGTACTTGAATTTGTGGGCAAGGAAACTATGGATTTACTGATTACAGAGACTGGTATTGGAGCTGAGAAGGATAGGGTAGACTTTAAGGATCAAGTACTTGAGGAGGTTACATTTGATCGATGCTTTTACATTTATGGTGGTCCTGAGCAGCTTGAGGAATTGGAAGCATTGGCTAGCCACTATACTCTGTTGTTCAACAGGAGAAAGGGGAAATTGTCACCAGATCAAAAATCGCTGTATGACGGGAAGCTCAAACAAATCCAGCAACTTTTCAGCTTTGCTGATGAAATGAGTGGAAGTAAAGCAGAGTCTGACAAAGGGAAGAAAATAGATATCAAAACTGAAGGCAATGATGATGACATGAAGAATCTGCATAACTCAAGTGTCAGCAAAGCTGCTGATATGGCTACTGGGTTCACAAATGCTTTAGCGGGACTACACGTAAATGATATGATCCAGCGAACCGGTGGCAGGCTTGAATCTCTTCACTCAGAAGGAGTTCATAGACTTTCAGAGATGTGCTGTTTTGCAGTCACACATCTGCTTATCCTCGGTAAGTCCATGATATCTCATGCCAACAAAGTTCAGGATGAAGACACTGAGGCATTGAAAATCGAGTGGGCAGAGGATCCTACAGAGAAAGCTAAGCTGATTAGAGGCAAGGCAGAAACAATGGCTGGATATGTTGAAGCAGTTTCCAATAGCTTTATAACAGGAATATCAGATGTATCTGAAACATACTCAGCTGCGATTAAAGGAGTTGCAGCTGCTGATTCCAAAGATGATCTTCTGAAAACATCGACCATGCAGGAAAAAGCAAGCACCTTCAACGATAGTCTTCGCTCTGACCAAACCACAGCTATCACAAAGATCCAGGAAGGGCTTCAGTACCTGTCCTATGTCGTGATTTCTACCTCGATGCCCTCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

512

Amino Acids

55.57

Weight (kDa)

4.65

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 250 - 452 5.1e-102 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1316
AccI GTMKAC 1 cut(s) 684
AccII CGCG 1 cut(s) 110
AciI CCGC 3 cut(s) 185, 212, 1037
AclWI GGATC 6 cut(s) 556, 702, 1054, 1229, 1242, 1471
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 3 cut(s) 321, 969, 1475
AfaI GTAC 4 cut(s) 124, 609, 702, 1496
AfiI CCNNNNNNNGG 3 cut(s) 62, 132, 1077
AflIII ACRYGT 1 cut(s) 1045
AgeI ACCGGT 3 cut(s) 55, 125, 1070
AgsI TTSAA 8 cut(s) 412, 509, 614, 802, 1085, 1216, 1295, 1435
AjnI CCWGG 2 cut(s) 402, 1479
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 3 cut(s) 566, 567, 647
Alw44I GTGCAC 1 cut(s) 555
AlwI GGATC 6 cut(s) 556, 702, 1054, 1229, 1242, 1471
AlwNI CAGNNNCTG 4 cut(s) 656, 917, 1376, 1498
Ama87I CYCGRG 1 cut(s) 74
ApaLI GTGCAC 1 cut(s) 555
ApeKI GCWGC 9 cut(s) 182, 206, 209, 227, 754, 1001, 1352, 1370, 1373
ApoI RAATTY 1 cut(s) 614
AsiGI ACCGGT 3 cut(s) 55, 125, 1070
Asp700I GAANNNNTTC 5 cut(s) 57, 416, 1089, 1104, 1488
AspA2I CCTAGG 1 cut(s) 536
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 746
AsuHPI GGTGA 1 cut(s) 816
AsuNHI GCTAGC 1 cut(s) 779
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 746
AvrII CCTAGG 1 cut(s) 536
BaeGI GKGCMC 2 cut(s) 488, 559
BamHI GGATCC 1 cut(s) 1234
BanII GRGCYC 1 cut(s) 75
BauI CACGAG 1 cut(s) 23
BbsI GAAGAC 4 cut(s) 15, 270, 1206, 1436
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 9 cut(s) 169, 193, 221, 239, 766, 988, 1339, 1360, 1382
BccI CCATC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 583, 617
BciT130I CCWGG 2 cut(s) 404, 1481
BcoDI GTCTC 3 cut(s) 566, 567, 647
BfaI CTAG 3 cut(s) 537, 569, 780
BfmI CTRYAG 2 cut(s) 207, 1239
BisI GCNGC 9 cut(s) 183, 207, 210, 228, 755, 1002, 1353, 1371, 1374
BlnI CCTAGG 1 cut(s) 536
BlpI GCTNAGC 1 cut(s) 1251
BlsI GCNGC 9 cut(s) 184, 208, 211, 229, 756, 1003, 1354, 1372, 1375
BmcAI AGTACT 2 cut(s) 609, 702
Bme1390I CCNGG 2 cut(s) 404, 1481
Bme18I GGWCC 1 cut(s) 746
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 1 cut(s) 1236
BmrFI CCNGG 2 cut(s) 404, 1481
BmrI ACTGGG 1 cut(s) 1026
BmsI GCATC 2 cut(s) 716, 1515
BmtI GCTAGC 1 cut(s) 783
BmuI ACTGGG 1 cut(s) 1026
BpiI GAAGAC 4 cut(s) 15, 270, 1206, 1436
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 2 cut(s) 282, 750
Bpu1102I GCTNAGC 1 cut(s) 1251
BpuEI CTTGAG 4 cut(s) 383, 725, 779, 970
Bsa29I ATCGAT 1 cut(s) 724
BsaAI YACGTR 1 cut(s) 1048
BsaJI CCNNGG 3 cut(s) 403, 536, 1156
BsaWI WCCGGW 3 cut(s) 55, 125, 1070
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 132, 1077
Bse118I RCCGGY 3 cut(s) 55, 125, 1070
Bse1I ACTGG 2 cut(s) 661, 1021
Bse3DI GCAATG 2 cut(s) 237, 961
BseBI CCWGG 2 cut(s) 404, 1481
BseCI ATCGAT 1 cut(s) 724
BseDI CCNNGG 3 cut(s) 403, 536, 1156
BseGI GGATG 2 cut(s) 265, 1201
BseLI CCNNNNNNNGG 3 cut(s) 62, 132, 1077
BseMI GCAATG 2 cut(s) 237, 961
BseMII CTCAG 5 cut(s) 660, 741, 1110, 1197, 1362
BseNI ACTGG 2 cut(s) 661, 1021
BseRI GAGGAG 2 cut(s) 41, 722
BseSI GKGCMC 2 cut(s) 488, 559
BseXI GCAGC 9 cut(s) 169, 193, 221, 239, 766, 988, 1339, 1360, 1382
Bsh1236I CGCG 1 cut(s) 110
BshTI ACCGGT 3 cut(s) 55, 125, 1070
BshVI ATCGAT 1 cut(s) 724
BsiHKAI GWGCWC 1 cut(s) 559
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 56, 126, 1071
BslFI GGGAC 1 cut(s) 1053
BslI CCNNNNNNNGG 3 cut(s) 62, 132, 1077
BsmAI GTCTC 3 cut(s) 566, 567, 647
BsmBI CGTCTC 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 1053
BsmI GAATGC 2 cut(s) 343, 606
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 3 cut(s) 75, 488, 559
Bsp143I GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
Bsp1720I GCTNAGC 1 cut(s) 1251
BspACI CCGC 3 cut(s) 185, 212, 1037
BspCNI CTCAG 5 cut(s) 661, 742, 1109, 1198, 1361
BspDI ATCGAT 1 cut(s) 724
BspFNI CGCG 1 cut(s) 110
BspLI GGNNCC 1 cut(s) 1236
BspMAI CTGCAG 1 cut(s) 211
BspOI GCTAGC 1 cut(s) 783
BspPI GGATC 6 cut(s) 556, 702, 1054, 1229, 1242, 1471
BspQI GCTCTTC 1 cut(s) 63
BsrDI GCAATG 2 cut(s) 237, 961
BsrFI RCCGGY 3 cut(s) 55, 125, 1070
BsrI ACTGG 2 cut(s) 661, 1021
BssAI RCCGGY 3 cut(s) 55, 125, 1070
BssECI CCNNGG 3 cut(s) 403, 536, 1156
BssMI GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 536
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 404, 1481
Bst4CI ACNGT 3 cut(s) 94, 515, 561
Bst6I CTCTTC 5 cut(s) 63, 113, 285, 381, 1095
BstAPI GCANNNNNTGC 1 cut(s) 590
BstBAI YACGTR 1 cut(s) 1048
BstC8I GCNNGC 4 cut(s) 459, 781, 1079, 1426
BstDEI CTNAG 8 cut(s) 282, 398, 669, 750, 1096, 1206, 1251, 1348
BstF5I GGATG 2 cut(s) 265, 1201
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 9 cut(s) 172, 551, 697, 724, 832, 1062, 1237, 1393, 1479
BstMAI GTCTC 3 cut(s) 566, 567, 647
BstMBI GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
BstMWI GCNNNNNNNGC 5 cut(s) 215, 224, 458, 590, 1010
BstNI CCWGG 2 cut(s) 404, 1481
BstSCI CCNGG 2 cut(s) 402, 1479
BstSFI CTRYAG 2 cut(s) 207, 1239
BstSLI GKGCMC 2 cut(s) 488, 559
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 9 cut(s) 169, 193, 221, 239, 766, 988, 1339, 1360, 1382
BstV2I GAAGAC 4 cut(s) 15, 270, 1206, 1436
BstX2I RGATCY 3 cut(s) 548, 1234, 1476
BstYI RGATCY 3 cut(s) 548, 1234, 1476
Bsu15I ATCGAT 1 cut(s) 724
BsuTUI ATCGAT 1 cut(s) 724
BtsCI GGATG 2 cut(s) 265, 1201
BtsIMutI CAGTG 3 cut(s) 99, 520, 1203
Cac8I GCNNGC 4 cut(s) 459, 781, 1079, 1426
CaiI CAGNNNCTG 4 cut(s) 656, 917, 1376, 1498
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 3 cut(s) 55, 125, 1070
Cfr13I GGNCC 1 cut(s) 746
ClaI ATCGAT 1 cut(s) 724
CseI GACGC 1 cut(s) 116
Csp6I GTAC 4 cut(s) 123, 608, 701, 1495
CspAI ACCGGT 3 cut(s) 55, 125, 1070
CviAII CATG 4 cut(s) 967, 1168, 1178, 1411
CviQI GTAC 4 cut(s) 123, 608, 701, 1495
DdeI CTNAG 8 cut(s) 282, 398, 669, 750, 1096, 1206, 1251, 1348
DpnI GATC 9 cut(s) 171, 550, 696, 723, 831, 1061, 1236, 1392, 1478
DpnII GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
Eam1104I CTCTTC 5 cut(s) 63, 113, 285, 381, 1095
EarI CTCTTC 5 cut(s) 63, 113, 285, 381, 1095
Eco130I CCWWGG 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 75
Eco32I GATATC 2 cut(s) 940, 1173
Eco47I GGWCC 1 cut(s) 746
Eco57I CTGAAG 3 cut(s) 321, 969, 1475
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 402, 1479
EcoRV GATATC 2 cut(s) 940, 1173
EcoT14I CCWWGG 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 536
Esp3I CGTCTC 1 cut(s) 567
FaeI CATG 4 cut(s) 970, 1171, 1181, 1414
FalI AAGNNNNNCTT 4 cut(s) 378, 410, 1377, 1409
FaqI GGGAC 1 cut(s) 1053
FatI CATG 4 cut(s) 966, 1167, 1177, 1410
FauI CCCGC 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 9 cut(s) 183, 207, 210, 228, 755, 1002, 1353, 1371, 1374
FokI GGATG 2 cut(s) 272, 1208
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 9 cut(s) 183, 207, 210, 228, 755, 1002, 1353, 1371, 1374
FspBI CTAG 3 cut(s) 537, 569, 780
GlaI GCGC 1 cut(s) 111
GluI GCNGC 9 cut(s) 183, 207, 210, 228, 755, 1002, 1353, 1371, 1374
GsuI CTGGAG 1 cut(s) 468
HapII CCGG 3 cut(s) 56, 126, 1071
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 112
Hin1II CATG 4 cut(s) 970, 1171, 1181, 1414
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 8 cut(s) 266, 272, 389, 425, 914, 973, 1085, 1379
HpaII CCGG 3 cut(s) 56, 126, 1071
HphI GGTGA 1 cut(s) 816
Hpy166II GTNNAC 4 cut(s) 106, 557, 685, 1023
Hpy188I TCNGA 9 cut(s) 169, 271, 919, 1099, 1120, 1330, 1339, 1398, 1453
Hpy188III TCNNGA 5 cut(s) 362, 447, 749, 1193, 1510
Hpy8I GTNNAC 4 cut(s) 106, 557, 685, 1023
HpyAV CCTTC 6 cut(s) 406, 667, 944, 1094, 1441, 1478
HpyCH4III ACNGT 3 cut(s) 94, 515, 561
HpyCH4IV ACGT 1 cut(s) 1047
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 224, 458, 590, 1010
HpyF3I CTNAG 8 cut(s) 282, 398, 669, 750, 1096, 1206, 1251, 1348
HpySE526I ACGT 1 cut(s) 1047
Hsp92II CATG 4 cut(s) 970, 1171, 1181, 1414
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 2 cut(s) 449, 665
Lsp1109I GCAGC 9 cut(s) 169, 193, 221, 239, 766, 988, 1339, 1360, 1382
LweI GCATC 2 cut(s) 716, 1515
MaeI CTAG 3 cut(s) 537, 569, 780
MaeII ACGT 1 cut(s) 1047
MaeIII GTNAC 4 cut(s) 390, 712, 822, 1138
MalI GATC 9 cut(s) 171, 550, 696, 723, 831, 1061, 1236, 1392, 1478
MboI GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
MflI RGATCY 3 cut(s) 548, 1234, 1476
MhlI GDGCHC 3 cut(s) 75, 488, 559
MluCI AATT 4 cut(s) 86, 614, 764, 818
MlyI GAGTC 3 cut(s) 260, 398, 923
MroXI GAANNNNTTC 5 cut(s) 57, 416, 1089, 1104, 1488
MseI TTAA 6 cut(s) 45, 347, 476, 587, 690, 1359
MslI CAYNNNNRTG 1 cut(s) 1050
MspA1I CMGCKG 3 cut(s) 212, 1352, 1373
MspI CCGG 3 cut(s) 56, 126, 1071
MspR9I CCNGG 2 cut(s) 404, 1481
Mva1269I GAATGC 2 cut(s) 343, 606
MvaI CCWGG 2 cut(s) 404, 1481
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 5 cut(s) 215, 224, 458, 590, 1010
NdeII GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
NheI GCTAGC 1 cut(s) 779
NlaIII CATG 4 cut(s) 970, 1171, 1181, 1414
NlaIV GGNNCC 1 cut(s) 1236
NmuCI GTSAC 3 cut(s) 390, 822, 1138
PciSI GCTCTTC 1 cut(s) 63
PctI GAATGC 2 cut(s) 343, 606
PdmI GAANNNNTTC 5 cut(s) 57, 416, 1089, 1104, 1488
PfeI GAWTC 5 cut(s) 272, 425, 973, 1085, 1379
PfoI TCCNGGA 1 cut(s) 1479
PinAI ACCGGT 3 cut(s) 55, 125, 1070
PkrI GCNGC 9 cut(s) 184, 208, 211, 229, 756, 1003, 1354, 1372, 1375
PleI GAGTC 3 cut(s) 260, 397, 922
PpsI GAGTC 3 cut(s) 260, 397, 922
Ppu21I YACGTR 1 cut(s) 1048
PsiI TTATAA 1 cut(s) 1316
Psp6I CCWGG 2 cut(s) 402, 1479
PspGI CCWGG 2 cut(s) 402, 1479
PspN4I GGNNCC 1 cut(s) 1236
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 4 cut(s) 656, 917, 1376, 1498
PsuI RGATCY 3 cut(s) 548, 1234, 1476
PvuII CAGCTG 2 cut(s) 1352, 1373
RsaI GTAC 4 cut(s) 124, 609, 702, 1496
RsaNI GTAC 4 cut(s) 123, 608, 701, 1495
RseI CAYNNNNRTG 1 cut(s) 1050
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 6 cut(s) 45, 347, 476, 587, 690, 1359
SatI GCNGC 9 cut(s) 183, 207, 210, 228, 755, 1002, 1353, 1371, 1374
Sau3AI GATC 9 cut(s) 169, 548, 694, 721, 829, 1059, 1234, 1390, 1476
Sau96I GGNCC 1 cut(s) 746
ScaI AGTACT 2 cut(s) 609, 702
SchI GAGTC 3 cut(s) 260, 398, 923
ScrFI CCNGG 2 cut(s) 404, 1481
SduI GDGCHC 3 cut(s) 75, 488, 559
SfaNI GCATC 2 cut(s) 716, 1515
SfcI CTRYAG 2 cut(s) 207, 1239
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 1050
SmlI CTYRAG 4 cut(s) 362, 704, 758, 985
SmoI CTYRAG 4 cut(s) 362, 704, 758, 985
Sse9I AATT 4 cut(s) 86, 614, 764, 818
SsiI CCGC 3 cut(s) 185, 212, 1037
SspMI CTAG 3 cut(s) 537, 569, 780
StyD4I CCNGG 2 cut(s) 402, 1479
StyI CCWWGG 1 cut(s) 536
TaaI ACNGT 3 cut(s) 94, 515, 561
TaiI ACGT 1 cut(s) 1050
TaqI TCGA 5 cut(s) 420, 724, 1221, 1406, 1523
TasI AATT 4 cut(s) 86, 614, 764, 818
TatI WGTACW 2 cut(s) 607, 700
TfiI GAWTC 5 cut(s) 272, 425, 973, 1085, 1379
Tru1I TTAA 6 cut(s) 45, 347, 476, 587, 690, 1359
Tru9I TTAA 6 cut(s) 45, 347, 476, 587, 690, 1359
TscAI CASTG 3 cut(s) 99, 520, 1210
TseFI GTSAC 3 cut(s) 390, 822, 1138
TseI GCWGC 9 cut(s) 182, 206, 209, 227, 754, 1001, 1352, 1370, 1373
Tsp45I GTSAC 3 cut(s) 390, 822, 1138
TspDTI ATGAA 5 cut(s) 276, 906, 983, 1097, 1212
TspRI CASTG 3 cut(s) 99, 520, 1210
VneI GTGCAC 1 cut(s) 555
VpaK11BI GGWCC 1 cut(s) 746
XapI RAATTY 1 cut(s) 614
XcmI CCANNNNNNNNNTGG 1 cut(s) 1070
XmaJI CCTAGG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 684
XmnI GAANNNNTTC 5 cut(s) 57, 416, 1089, 1104, 1488
XspI CTAG 3 cut(s) 537, 569, 780
ZrmI AGTACT 2 cut(s) 609, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.