Prupe.8G114000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0008704
Physical Location & Seq
Forward (+)
14209505 .. 14213490
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G114000.1

Sequence Viewer

Length: 1335 bp
ATGGAGGAAGAAGACAAACAGCCACTGAAGGAAGAGAAAAGTCTTCTACAAGAAGAAAAAGAGGAAGCAAAAAAAGCAGAAGCAGCTGAAGCACAAGCATCACCGGCAAAAAGTGGAGGATGGGGAGGCTGGGGATTTTCCTCTGTTTTCTCAGATCTCCAGAAAGCCGCCGAAGAGATCTCTCGCAATGCGGCTGCAGTTGCTCAGACTGCAGCAAAGAGCATTGCCGACATGCAAAACCCTGCAGAGGACTCGGAAGAATCTTCCAAGGAGGAGGAAGGGGATACTGAAAAGGAAAGCCAGGATGGGACTGAAAGCCAAGATGAGCATGAAAAGCAACGAAAGGCTGCTCTAGAAAAATTAGAAAAAGCTAGTGAAGATTCGTTTCTCGGTCAGGGTTTGAAGGTACTTGATAGTTCTGTGGAGAATTTTGCTTCAGGAGCATGGCAAGCATTAGGAAGTGCATTGAAAGGCAGTACTGATCTAGTCCACAAGCTTGAGAATTCAGCTGTGAATCTTGCGGGATCCATCCAGCAAGGTGGTTTGCCTACGGCTGCTGGTTCTGCTGCGCCATCCTTATTAGAGACTGGAAAGGCTTTTACCTCTAAGGGAATGCAAGTGCTTGAGCTTGTAGGCAAGGAAACTTTGGATCTACTGATCACAGAGACTGGTATAGAAGTTGAGAAGAATTCTAAACCCTCTGAACAGGAAGCTGAAGAGGATCAGTTATACGAGGAAGTAGGATTTGATCGATGCTTCTACATATATGGAGGTCCAGAACAGTTGGAGAATCTTCTGTATTTAACATTATTCAGGTTCACAAGTGCTTTAGCTGGACAAGCTGTTAATGATATAATTCAAAGAACCACCGGCCGATTAGAGAGTCTTCACTCAGAGGGAGTTCATAGACTCTCGGAAATGTGCTGCTCTGCGGTGTCTCAACTGTTGATGCTTGGTAAATCCATCATATCTAGTGCAAACAACACTCAGGTGGAAGATGTGGATGCTGATGTCGAGAATATTGATTGGCCTGAAGATTCTGTTGAGAAAGCAAAGATAATCAGATCAAAAGCAGAAGCAATGACTGGATATGTGGAGGCAGTGTCCAGCAGTTTCATTACAGGCATATCTGATGTTGCCGAAGCTTATCTAGCAGCCATAAAAGGTGCTTCTGCGGAATCCCATGACGTGCTTCCACAGACATCAATCCAAGAAAAAGCCAGTTCCTTCTCTAAACATCTCCGTGATGACCAGAACACAGCTTTGGGCAAAATCCAGGACGGGCTTCACTACTTGTCGTGTGTGGTTGTTTCAAGCTCTATGCCCACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

445

Amino Acids

47.66

Weight (kDa)

4.5

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 168, 191, 521, 932, 1175
AclWI GGATC 4 cut(s) 519, 532, 657, 729
AcoI YGGCCR 1 cut(s) 871
AcsI RAATTY 3 cut(s) 427, 502, 688
AcuI CTGAAG 5 cut(s) 47, 108, 420, 735, 1053
AfaI GTAC 2 cut(s) 408, 478
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 4 cut(s) 403, 469, 860, 1314
AjiI CACGTC 1 cut(s) 1189
AjnI CCWGG 2 cut(s) 300, 1275
AjuI GAANNNNNNNTTGG 2 cut(s) 1247, 1279
AleI CACNNNNGTG 1 cut(s) 989
Alw26I GTCTC 3 cut(s) 578, 659, 942
AlwI GGATC 4 cut(s) 519, 532, 657, 729
AlwNI CAGNNNCTG 2 cut(s) 25, 668
AoxI GGCC 2 cut(s) 871, 1028
ApeKI GCWGC 8 cut(s) 83, 194, 212, 347, 554, 566, 924, 1154
ApoI RAATTY 3 cut(s) 427, 502, 688
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 773
AsuHPI GGTGA 1 cut(s) 93
AvaII GGWCC 1 cut(s) 773
BamHI GGATCC 1 cut(s) 524
BbsI GAAGAC 3 cut(s) 18, 35, 878
BbvI GCAGC 8 cut(s) 95, 181, 224, 334, 541, 553, 911, 1166
BccI CCATC 5 cut(s) 114, 299, 536, 580, 971
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 2 cut(s) 302, 1277
BciVI GTATCC 1 cut(s) 277
BclI TGATCA 1 cut(s) 657
BcoDI GTCTC 3 cut(s) 578, 659, 942
BfaI CTAG 5 cut(s) 353, 372, 485, 972, 1151
BfmI CTRYAG 3 cut(s) 195, 210, 243
BfuI GTATCC 1 cut(s) 277
BglII AGATCT 2 cut(s) 154, 177
BmcAI AGTACT 1 cut(s) 478
Bme1390I CCNGG 2 cut(s) 302, 1277
Bme18I GGWCC 1 cut(s) 773
BmgBI CACGTC 1 cut(s) 1189
BmgT120I GGNCC 1 cut(s) 773
BmiI GGNNCC 1 cut(s) 526
BmrFI CCNGG 2 cut(s) 302, 1277
BmsI GCATC 4 cut(s) 107, 743, 939, 994
BpiI GAAGAC 3 cut(s) 18, 35, 878
BpmI CTGGAG 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 518, 644
Bsa29I ATCGAT 1 cut(s) 751
BsaJI CCNNGG 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse118I RCCGGY 2 cut(s) 103, 869
Bse1I ACTGG 4 cut(s) 592, 673, 1090, 1221
Bse3DI GCAATG 3 cut(s) 193, 222, 1086
BseBI CCWGG 2 cut(s) 302, 1277
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 5 cut(s) 125, 310, 528, 572, 1009
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 3 cut(s) 193, 222, 1086
BseMII CTCAG 4 cut(s) 165, 218, 906, 1001
BseNI ACTGG 4 cut(s) 592, 673, 1090, 1221
BseRI GAGGAG 1 cut(s) 287
BseX3I CGGCCG 1 cut(s) 871
BseXI GCAGC 8 cut(s) 95, 181, 224, 334, 541, 553, 911, 1166
BseYI CCCAGC 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 874
BshFI GGCC 2 cut(s) 873, 1030
BshVI ATCGAT 1 cut(s) 751
BsiEI CGRYCG 1 cut(s) 874
BsiSI CCGG 2 cut(s) 104, 870
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 3 cut(s) 578, 659, 942
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 618
BsnI GGCC 2 cut(s) 873, 1030
Bsp143I GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
BspACI CCGC 5 cut(s) 168, 191, 521, 932, 1175
BspANI GGCC 2 cut(s) 873, 1030
BspCNI CTCAG 4 cut(s) 164, 217, 905, 1000
BspDI ATCGAT 1 cut(s) 751
BspLI GGNNCC 1 cut(s) 526
BspMAI CTGCAG 3 cut(s) 199, 214, 247
BspPI GGATC 4 cut(s) 519, 532, 657, 729
BsrDI GCAATG 3 cut(s) 193, 222, 1086
BsrFI RCCGGY 2 cut(s) 103, 869
BsrI ACTGG 4 cut(s) 592, 673, 1090, 1221
BssAI RCCGGY 2 cut(s) 103, 869
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 2 cut(s) 302, 1277
Bst4CI ACNGT 2 cut(s) 783, 945
Bst6I CTCTTC 3 cut(s) 27, 168, 711
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 5 cut(s) 151, 204, 606, 892, 987
BstF5I GGATG 5 cut(s) 125, 310, 528, 572, 1009
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 9 cut(s) 157, 180, 484, 527, 652, 660, 724, 751, 1067
BstMAI GTCTC 3 cut(s) 578, 659, 942
BstMBI GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
BstMCI CGRYCG 1 cut(s) 874
BstNI CCWGG 2 cut(s) 302, 1277
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 300, 1275
BstSFI CTRYAG 3 cut(s) 195, 210, 243
BstV1I GCAGC 8 cut(s) 95, 181, 224, 334, 541, 553, 911, 1166
BstV2I GAAGAC 3 cut(s) 18, 35, 878
BstX2I RGATCY 4 cut(s) 154, 177, 524, 649
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 4 cut(s) 154, 177, 524, 649
BstZI CGGCCG 1 cut(s) 871
Bsu15I ATCGAT 1 cut(s) 751
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 2 cut(s) 873, 1030
BsuTUI ATCGAT 1 cut(s) 751
BtrI CACGTC 1 cut(s) 1189
BtsCI GGATG 5 cut(s) 125, 310, 528, 572, 1009
BtsI GCAGTG 2 cut(s) 1107, 1326
BtsIMutI CAGTG 3 cut(s) 23, 1107, 1326
Cac8I GCNNGC 1 cut(s) 450
CaiI CAGNNNCTG 2 cut(s) 25, 668
CfoI GCGC 1 cut(s) 571
Cfr10I RCCGGY 2 cut(s) 103, 869
Cfr13I GGNCC 1 cut(s) 773
ClaI ATCGAT 1 cut(s) 751
Csp6I GTAC 2 cut(s) 407, 477
CviAII CATG 5 cut(s) 232, 329, 444, 1184, 1332
CviQI GTAC 2 cut(s) 407, 477
DdeI CTNAG 5 cut(s) 151, 204, 606, 892, 987
DpnI GATC 9 cut(s) 156, 179, 483, 526, 651, 659, 723, 750, 1066
DpnII GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
EaeI YGGCCR 1 cut(s) 871
EagI CGGCCG 1 cut(s) 871
Eam1104I CTCTTC 3 cut(s) 27, 168, 711
EarI CTCTTC 3 cut(s) 27, 168, 711
EclXI CGGCCG 1 cut(s) 871
Eco130I CCWWGG 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 773
Eco52I CGGCCG 1 cut(s) 871
Eco57I CTGAAG 5 cut(s) 47, 108, 420, 735, 1053
EcoRI GAATTC 2 cut(s) 502, 688
EcoRII CCWGG 2 cut(s) 300, 1275
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 5 cut(s) 235, 332, 447, 1187, 1335
FaqI GGGAC 1 cut(s) 322
FatI CATG 5 cut(s) 231, 328, 443, 1183, 1331
FauI CCCGC 1 cut(s) 514
FbaI TGATCA 1 cut(s) 657
FokI GGATG 5 cut(s) 132, 317, 515, 559, 1016
FspBI CTAG 5 cut(s) 353, 372, 485, 972, 1151
GlaI GCGC 1 cut(s) 570
GsaI CCCAGC 1 cut(s) 133
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 2 cut(s) 873, 1030
HapII CCGG 2 cut(s) 104, 870
HhaI GCGC 1 cut(s) 571
Hin1II CATG 5 cut(s) 235, 332, 447, 1187, 1335
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HindIII AAGCTT 2 cut(s) 494, 1143
HinfI GANTC 9 cut(s) 251, 260, 380, 514, 790, 883, 909, 1037, 1178
HpaII CCGG 2 cut(s) 104, 870
HphI GGTGA 1 cut(s) 93
Hpy166II GTNNAC 2 cut(s) 490, 819
Hpy188I TCNGA 8 cut(s) 154, 207, 256, 703, 895, 916, 1064, 1132
Hpy188III TCNNGA 5 cut(s) 160, 353, 438, 776, 1015
Hpy8I GTNNAC 2 cut(s) 490, 819
HpyAV CCTTC 4 cut(s) 22, 272, 397, 1237
HpyCH4III ACNGT 2 cut(s) 783, 945
HpyCH4IV ACGT 1 cut(s) 1188
HpyCH4V TGCA 8 cut(s) 197, 212, 235, 245, 464, 616, 977, 1331
HpyF3I CTNAG 5 cut(s) 151, 204, 606, 892, 987
HpySE526I ACGT 1 cut(s) 1188
Hsp92II CATG 5 cut(s) 235, 332, 447, 1187, 1335
HspAI GCGC 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 8 cut(s) 95, 181, 224, 334, 541, 553, 911, 1166
LweI GCATC 4 cut(s) 107, 743, 939, 994
MaeI CTAG 5 cut(s) 353, 372, 485, 972, 1151
MaeII ACGT 1 cut(s) 1188
MalI GATC 9 cut(s) 156, 179, 483, 526, 651, 659, 723, 750, 1066
MboI GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
MflI RGATCY 4 cut(s) 154, 177, 524, 649
MluCI AATT 5 cut(s) 359, 427, 502, 688, 855
MlyI GAGTC 3 cut(s) 245, 892, 903
MmeI TCCRAC 1 cut(s) 765
MseI TTAA 2 cut(s) 803, 846
MslI CAYNNNNRTG 2 cut(s) 989, 1242
MspA1I CMGCKG 2 cut(s) 86, 509
MspI CCGG 2 cut(s) 104, 870
MspR9I CCNGG 2 cut(s) 302, 1277
Mva1269I GAATGC 1 cut(s) 618
MvaI CCWGG 2 cut(s) 302, 1277
NdeII GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
NlaIII CATG 5 cut(s) 235, 332, 447, 1187, 1335
NlaIV GGNNCC 1 cut(s) 526
NspI RCATGY 1 cut(s) 235
OliI CACNNNNGTG 1 cut(s) 989
PctI GAATGC 1 cut(s) 618
PfeI GAWTC 6 cut(s) 260, 380, 514, 790, 1037, 1178
PfoI TCCNGGA 1 cut(s) 1275
PleI GAGTC 3 cut(s) 245, 891, 903
PpsI GAGTC 3 cut(s) 245, 891, 903
Psp6I CCWGG 2 cut(s) 300, 1275
PspFI CCCAGC 1 cut(s) 129
PspGI CCWGG 2 cut(s) 300, 1275
PspN4I GGNNCC 1 cut(s) 526
PspPI GGNCC 1 cut(s) 773
PstI CTGCAG 3 cut(s) 199, 214, 247
PstNI CAGNNNCTG 2 cut(s) 25, 668
PsuI RGATCY 4 cut(s) 154, 177, 524, 649
PvuII CAGCTG 2 cut(s) 86, 509
RsaI GTAC 2 cut(s) 408, 478
RsaNI GTAC 2 cut(s) 407, 477
RseI CAYNNNNRTG 2 cut(s) 989, 1242
SaqAI TTAA 2 cut(s) 803, 846
Sau3AI GATC 9 cut(s) 154, 177, 481, 524, 649, 657, 721, 748, 1064
Sau96I GGNCC 1 cut(s) 773
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 3 cut(s) 245, 892, 903
ScrFI CCNGG 2 cut(s) 302, 1277
SfaNI GCATC 4 cut(s) 107, 743, 939, 994
SfcI CTRYAG 3 cut(s) 195, 210, 243
SinI GGWCC 1 cut(s) 773
SmiMI CAYNNNNRTG 2 cut(s) 989, 1242
SmlI CTYRAG 2 cut(s) 497, 623
SmoI CTYRAG 2 cut(s) 497, 623
Sse9I AATT 5 cut(s) 359, 427, 502, 688, 855
SsiI CCGC 5 cut(s) 168, 191, 521, 932, 1175
SspI AATATT 1 cut(s) 1021
SspMI CTAG 5 cut(s) 353, 372, 485, 972, 1151
StyD4I CCNGG 2 cut(s) 300, 1275
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 783, 945
TaiI ACGT 1 cut(s) 1191
TaqI TCGA 2 cut(s) 751, 1014
TaqII GACCGA 1 cut(s) 380
TasI AATT 5 cut(s) 359, 427, 502, 688, 855
TatI WGTACW 1 cut(s) 476
TauI GCSGC 2 cut(s) 170, 194
TfiI GAWTC 6 cut(s) 260, 380, 514, 790, 1037, 1178
Tru1I TTAA 2 cut(s) 803, 846
Tru9I TTAA 2 cut(s) 803, 846
TscAI CASTG 3 cut(s) 30, 1107, 1333
TseI GCWGC 8 cut(s) 83, 194, 212, 347, 554, 566, 924, 1154
TspDTI ATGAA 3 cut(s) 345, 893, 1105
TspGWI ACGGA 1 cut(s) 1232
TspRI CASTG 3 cut(s) 30, 1107, 1333
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 3 cut(s) 427, 502, 688
XbaI TCTAGA 1 cut(s) 352
XceI RCATGY 1 cut(s) 235
XspI CTAG 5 cut(s) 353, 372, 485, 972, 1151
ZrmI AGTACT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.