MD10G1088000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008704
Physical Location & Seq
Forward (+)
13049032 .. 13053619
4588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1088000.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGGAGGAAGAAGACAAACAGCCGCTCAAGGAGGACAAGGGTCCTCAACAGGAAGAGAAAGAAGAACCAAAGGGAGGAGCAGCTGAAGCACAAGTGTCGCCGGCGAAAAGTGGCGGAGGATGGGGAGGTTGGGGGTTTTCGTCCGTCTTCTCAGATCTCCAGAAAGCCGCCGAAGAGATCTCCCGCAATGCGGCTGCGGCTGCTCAGACGGCAGCGAAGAGCCTTGCCGACATGCAAGCTGCTGAGGACTCAGAAGAATCTTCCAAGGAGGAAGAAGGGGATACTGAAAAGGAAACCCAAGATGACACTGAGAGCCAAGATGAGCATGAAAAGCAAAGAAAGGCTGCTCTTGATAAATTGGAGAAAGCTAGTGAGGATTCGCTTTTCGGTCAGGGTTTGAAAGTGCTTGATAGTTCCGTGGAGAATTTTGCTACAGGAGCGTGGCAAGCATTAGGAAGTGCATTGAAAGGGGGTACCGACCTAGTGCACAAGATTGAGAATTCTGCTGTGAACCTTGCGGGATCTATCCAGCAAGGTGGAGGTGCTGGTTCTACCGCGCCATCCTTATTAGAGACTGGAAAAGCTTTTACATCAAAGGGAATGCAAGTGCTTGAGCTTGTAGGAAAGGAAACTTTGGATCTACTGATCACAGAGACTGGTATAGAAGTTGATAAGGATTCTACACTTTCTGAAAAGGAAACTGAAGAGGATCAGTTATATGAGGAAGTGGGATTTGATCGGTGTTTCTACATATATGGAGGTCCAGAACAGTTGGAGGAACTGGAGGCTTTGTCCAGCCATTATGCGCTATTATTTAATCGGAGAAAAGGAAAGTTGTCATCAGAACAGAAGTCTATTTATGATGGAAAGCTTAAACAGGTGCAACAGATTTTCAGTCTGAATACTGAGTTGGATGGAAGTGGTGCAGAGTTGGACAAAGGCAAAAAGAAAAAGACCGGAGATGATGGAAATAGCAATGAGATGAAGAGTTTGCATGATTCAAGCGTCAGCAAGGCTGCTGATATGGCGGCTGGGTTCACAAGTGCCTTAGCAGGACAAGCTGTTAACGATATAATTCAAAGAACAACTGGTAGATTGGAGAGTCTTCACTCAGAGGGGGTTCATAGGCTGTCAGAATTGTGCTGTTCAGCAGTATCTCAACTGTTGATGCTTGGTAAATCCATCATATCTAGTGCAAACAAAACTCAGGCAGAAGATGTGGATGCCGATCTGGAGAATATCGATTGGCCTGAAGATTCTGTTGAGAAAGCAAAGATAATCAGATCAAAAACACAAACAATGACAGGATATGTGGAGGCAGTGTCTAGCAGTTTCGTCACAGGTATATCTGATGTTGCAGAAGCTTATCTAGCAGCCATAAAGGGCGCTTCTGAGGACTCCCACGAAGTGCTCCCACGTACATCGATCCAAGAAAAAGCAAGTTCTTTCTCTAAACACCTTCGTGATGACCAGACCACAGCTTTGAGCAAAATCCAGGATGGGCTACACTACTTGTCGTACGTTGTCATTTCAACCTCTATGCCTGCAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

518

Amino Acids

55.46

Weight (kDa)

4.63

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 243 - 514 1.4e-141 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 473
AccBSI CCGCTC 1 cut(s) 25
AccII CGCG 1 cut(s) 557
AciI CCGC 9 cut(s) 23, 114, 168, 184, 191, 197, 518, 555, 1028
AclWI GGATC 4 cut(s) 529, 645, 717, 1420
AcsI RAATTY 2 cut(s) 424, 499
AcuI CTGAAG 3 cut(s) 105, 723, 1272
AdeI CACNNNGTG 1 cut(s) 1408
AfaI GTAC 3 cut(s) 475, 1420, 1520
AfiI CCNNNNNNNGG 2 cut(s) 74, 190
AgsI TTSAA 5 cut(s) 400, 466, 1002, 1079, 1533
AjnI CCWGG 1 cut(s) 1494
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AleI CACNNNNGTG 1 cut(s) 1461
AluBI AGCT 9 cut(s) 83, 239, 368, 584, 616, 871, 1061, 1364, 1481
AluI AGCT 9 cut(s) 83, 239, 368, 584, 616, 871, 1061, 1364, 1481
Alw21I GWGCWC 2 cut(s) 489, 1413
Alw26I GTCTC 2 cut(s) 566, 647
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 4 cut(s) 529, 645, 717, 1420
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 1 cut(s) 1247
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 9 cut(s) 80, 194, 200, 212, 239, 344, 1016, 1373, 1547
ApoI RAATTY 2 cut(s) 424, 499
ArsI GACNNNNNNTTYG 2 cut(s) 1465, 1497
Asp718I GGTACC 1 cut(s) 473
AspLEI GCGC 3 cut(s) 559, 808, 1388
AspS9I GGNCC 2 cut(s) 41, 761
AvaII GGWCC 2 cut(s) 41, 761
BaeGI GKGCMC 1 cut(s) 489
BanI GGYRCC 1 cut(s) 473
BbsI GAAGAC 3 cut(s) 18, 139, 1097
Bbv12I GWGCWC 2 cut(s) 489, 1413
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 8 cut(s) 92, 181, 187, 224, 226, 331, 1003, 1385
BccI CCATC 7 cut(s) 114, 568, 857, 908, 959, 1190, 1493
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 1 cut(s) 1496
BciVI GTATCC 1 cut(s) 274
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 566, 647
BfaI CTAG 5 cut(s) 369, 482, 1191, 1326, 1370
BfmI CTRYAG 2 cut(s) 432, 1545
BfoI RGCGCY 1 cut(s) 1389
BfuI GTATCC 1 cut(s) 274
BglII AGATCT 2 cut(s) 154, 177
Bme1390I CCNGG 1 cut(s) 1496
Bme18I GGWCC 2 cut(s) 41, 761
BmgT120I GGNCC 2 cut(s) 41, 761
BmiI GGNNCC 2 cut(s) 42, 475
BmrFI CCNGG 1 cut(s) 1496
BmsI GCATC 2 cut(s) 1158, 1213
BoxI GACNNNNGTC 1 cut(s) 39
BpiI GAAGAC 3 cut(s) 18, 139, 1097
BpmI CTGGAG 3 cut(s) 143, 803, 1253
Bpu10I CCTNAGC 2 cut(s) 243, 1048
BpuEI CTTGAG 2 cut(s) 11, 632
Bsa29I ATCGAT 2 cut(s) 1242, 1424
BsaAI YACGTR 1 cut(s) 1418
BsaBI GATNNNNATC 1 cut(s) 1227
BsaJI CCNNGG 2 cut(s) 264, 417
BsaWI WCCGGW 1 cut(s) 956
BsaXI ACNNNNNCTCC 4 cut(s) 776, 806, 1307, 1337
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 190
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 4 cut(s) 580, 661, 786, 1093
Bse3DI GCAATG 2 cut(s) 193, 982
Bse8I GATNNNNATC 1 cut(s) 1227
BseBI CCWGG 1 cut(s) 1496
BseCI ATCGAT 2 cut(s) 1242, 1424
BseDI CCNNGG 2 cut(s) 264, 417
BseGI GGATG 5 cut(s) 125, 560, 919, 1228, 1504
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 2 cut(s) 74, 190
BseMI GCAATG 2 cut(s) 193, 982
BseMII CTCAG 9 cut(s) 165, 218, 234, 264, 300, 897, 1125, 1220, 1383
BseNI ACTGG 4 cut(s) 580, 661, 786, 1093
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 8 cut(s) 92, 181, 187, 224, 226, 331, 1003, 1385
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 1 cut(s) 1249
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 2 cut(s) 1242, 1424
BsiHKAI GWGCWC 2 cut(s) 489, 1413
BsiSI CCGG 2 cut(s) 101, 957
BsiWI CGTACG 1 cut(s) 1518
BslI CCNNNNNNNGG 2 cut(s) 74, 190
BsmAI GTCTC 2 cut(s) 566, 647
BsmI GAATGC 1 cut(s) 606
BsnI GGCC 1 cut(s) 1249
Bsp1286I GDGCHC 2 cut(s) 489, 1413
BspACI CCGC 9 cut(s) 23, 114, 168, 184, 191, 197, 518, 555, 1028
BspANI GGCC 1 cut(s) 1249
BspCNI CTCAG 9 cut(s) 164, 217, 235, 263, 301, 898, 1124, 1219, 1384
BspDI ATCGAT 2 cut(s) 1242, 1424
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 2 cut(s) 42, 475
BspMAI CTGCAG 1 cut(s) 1549
BspPI GGATC 4 cut(s) 529, 645, 717, 1420
BspQI GCTCTTC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 2 cut(s) 193, 982
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 4 cut(s) 580, 661, 786, 1093
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 2 cut(s) 264, 417
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 1496
Bst4CI ACNGT 2 cut(s) 771, 1164
Bst6I CTCTTC 5 cut(s) 48, 168, 212, 699, 980
BstBAI YACGTR 1 cut(s) 1418
BstC8I GCNNGC 4 cut(s) 102, 237, 447, 1545
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 5 cut(s) 125, 560, 919, 1228, 1504
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 1389
BstHHI GCGC 3 cut(s) 559, 808, 1388
BstMAI GTCTC 2 cut(s) 566, 647
BstNI CCWGG 1 cut(s) 1496
BstNSI RCATGY 1 cut(s) 235
BstPAI GACNNNNGTC 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 1494
BstSFI CTRYAG 2 cut(s) 432, 1545
BstSLI GKGCMC 1 cut(s) 489
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 8 cut(s) 92, 181, 187, 224, 226, 331, 1003, 1385
BstV2I GAAGAC 3 cut(s) 18, 139, 1097
BstX2I RGATCY 4 cut(s) 154, 177, 521, 637
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 4 cut(s) 154, 177, 521, 637
Bsu15I ATCGAT 2 cut(s) 1242, 1424
BsuI GTATCC 1 cut(s) 274
BsuRI GGCC 1 cut(s) 1249
BsuTUI ATCGAT 2 cut(s) 1242, 1424
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 5 cut(s) 125, 560, 919, 1228, 1504
BtsI GCAGTG 1 cut(s) 1326
BtsIMutI CAGTG 2 cut(s) 306, 1326
Cac8I GCNNGC 4 cut(s) 102, 237, 447, 1545
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 3 cut(s) 559, 808, 1388
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 2 cut(s) 41, 761
ClaI ATCGAT 2 cut(s) 1242, 1424
CseI GACGC 1 cut(s) 994
Csp6I GTAC 3 cut(s) 474, 1419, 1519
CviAII CATG 3 cut(s) 232, 326, 995
CviQI GTAC 3 cut(s) 474, 1419, 1519
DraIII CACNNNGTG 1 cut(s) 1408
Eam1104I CTCTTC 5 cut(s) 48, 168, 212, 699, 980
EarI CTCTTC 5 cut(s) 48, 168, 212, 699, 980
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 264
Eco47I GGWCC 2 cut(s) 41, 761
Eco57I CTGAAG 3 cut(s) 105, 723, 1272
EcoO109I RGGNCCY 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 1494
EcoT14I CCWWGG 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 3 cut(s) 235, 329, 998
FatI CATG 3 cut(s) 231, 325, 994
FauI CCCGC 2 cut(s) 191, 511
FbaI TGATCA 1 cut(s) 645
FokI GGATG 5 cut(s) 132, 547, 926, 1235, 1511
FspBI CTAG 5 cut(s) 369, 482, 1191, 1326, 1370
GlaI GCGC 3 cut(s) 558, 807, 1387
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 3 cut(s) 143, 803, 1253
HaeII RGCGCY 1 cut(s) 1389
HaeIII GGCC 1 cut(s) 1249
HapII CCGG 2 cut(s) 101, 957
HgaI GACGC 1 cut(s) 994
HhaI GCGC 3 cut(s) 559, 808, 1388
Hin1II CATG 3 cut(s) 235, 329, 998
Hin6I GCGC 3 cut(s) 557, 806, 1386
HinP1I GCGC 3 cut(s) 557, 806, 1386
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HindIII AAGCTT 3 cut(s) 582, 869, 1362
HinfI GANTC 8 cut(s) 248, 257, 377, 677, 998, 1102, 1256, 1397
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 2 cut(s) 101, 957
Hpy166II GTNNAC 4 cut(s) 487, 511, 1038, 1066
Hpy188III TCNNGA 5 cut(s) 160, 350, 764, 1232, 1463
Hpy8I GTNNAC 4 cut(s) 487, 511, 1038, 1066
HpyAV CCTTC 2 cut(s) 269, 1469
HpyCH4III ACNGT 2 cut(s) 771, 1164
HpyCH4IV ACGT 2 cut(s) 1417, 1521
HpySE526I ACGT 2 cut(s) 1417, 1521
Hsp92II CATG 3 cut(s) 235, 329, 998
HspAI GCGC 3 cut(s) 557, 806, 1386
KpnI GGTACC 1 cut(s) 477
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 645
KspAI GTTAAC 1 cut(s) 1066
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 3 cut(s) 77, 437, 1416
Lsp1109I GCAGC 8 cut(s) 92, 181, 187, 224, 226, 331, 1003, 1385
LweI GCATC 2 cut(s) 1158, 1213
MaeI CTAG 5 cut(s) 369, 482, 1191, 1326, 1370
MaeII ACGT 2 cut(s) 1417, 1521
MaeIII GTNAC 1 cut(s) 1336
MbiI CCGCTC 1 cut(s) 25
MflI RGATCY 4 cut(s) 154, 177, 521, 637
MhlI GDGCHC 2 cut(s) 489, 1413
MluCI AATT 5 cut(s) 356, 424, 499, 1074, 1136
MlyI GAGTC 3 cut(s) 242, 1111, 1391
MmeI TCCRAC 3 cut(s) 753, 891, 912
MreI CGCCGGCG 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 100
MseI TTAA 3 cut(s) 816, 873, 1065
MslI CAYNNNNRTG 1 cut(s) 1461
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 101, 957
MspR9I CCNGG 1 cut(s) 1496
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 1 cut(s) 1496
MvnI CGCG 1 cut(s) 557
NaeI GCCGGC 1 cut(s) 102
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 3 cut(s) 235, 329, 998
NlaIV GGNNCC 2 cut(s) 42, 475
NmuCI GTSAC 1 cut(s) 1336
NspI RCATGY 1 cut(s) 235
OliI CACNNNNGTG 1 cut(s) 1461
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 606
PdiI GCCGGC 1 cut(s) 102
PfeI GAWTC 5 cut(s) 257, 377, 677, 998, 1256
Pfl23II CGTACG 1 cut(s) 1518
PfoI TCCNGGA 1 cut(s) 1494
PleI GAGTC 3 cut(s) 242, 1110, 1391
PpsI GAGTC 3 cut(s) 242, 1110, 1391
Ppu21I YACGTR 1 cut(s) 1418
PpuMI RGGWCCY 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 1494
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 1494
PspLI CGTACG 1 cut(s) 1518
PspN4I GGNNCC 2 cut(s) 42, 475
PspPI GGNCC 2 cut(s) 41, 761
PspPPI RGGWCCY 1 cut(s) 41
PstI CTGCAG 1 cut(s) 1549
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 4 cut(s) 154, 177, 521, 637
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 3 cut(s) 475, 1420, 1520
RsaNI GTAC 3 cut(s) 474, 1419, 1519
RseI CAYNNNNRTG 1 cut(s) 1461
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 3 cut(s) 816, 873, 1065
Sau96I GGNCC 2 cut(s) 41, 761
SchI GAGTC 3 cut(s) 242, 1111, 1391
ScrFI CCNGG 1 cut(s) 1496
SduI GDGCHC 2 cut(s) 489, 1413
SfaNI GCATC 2 cut(s) 1158, 1213
SfcI CTRYAG 2 cut(s) 432, 1545
SgrAI CRCCGGYG 1 cut(s) 100
SinI GGWCC 2 cut(s) 41, 761
SmiMI CAYNNNNRTG 1 cut(s) 1461
SmlI CTYRAG 2 cut(s) 26, 611
SmoI CTYRAG 2 cut(s) 26, 611
Sse9I AATT 5 cut(s) 356, 424, 499, 1074, 1136
SsiI CCGC 9 cut(s) 23, 114, 168, 184, 191, 197, 518, 555, 1028
SspMI CTAG 5 cut(s) 369, 482, 1191, 1326, 1370
StyD4I CCNGG 1 cut(s) 1494
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 771, 1164
TaiI ACGT 2 cut(s) 1420, 1524
TaqI TCGA 2 cut(s) 1242, 1424
TaqII GACCGA 1 cut(s) 377
TasI AATT 5 cut(s) 356, 424, 499, 1074, 1136
TauI GCSGC 5 cut(s) 25, 170, 194, 200, 1031
TfiI GAWTC 5 cut(s) 257, 377, 677, 998, 1256
Tru1I TTAA 3 cut(s) 816, 873, 1065
Tru9I TTAA 3 cut(s) 816, 873, 1065
TscAI CASTG 2 cut(s) 313, 1326
TseFI GTSAC 1 cut(s) 1336
TseI GCWGC 9 cut(s) 80, 194, 200, 212, 239, 344, 1016, 1373, 1547
Tsp45I GTSAC 1 cut(s) 1336
TspDTI ATGAA 3 cut(s) 342, 998, 1112
TspGWI ACGGA 2 cut(s) 133, 406
TspRI CASTG 2 cut(s) 313, 1326
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 41, 761
XapI RAATTY 2 cut(s) 424, 499
XceI RCATGY 1 cut(s) 235
XspI CTAG 5 cut(s) 369, 482, 1191, 1326, 1370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.