RLG00000014521

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008704
Physical Location & Seq
Forward (+)
54315925 .. 54319544
3620 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014521

Sequence Viewer

Length: 1539 bp
ATGGAGGAAGAGAAGCAGCAACTTAAGGACGAGAAAGCCCCTTTGCAACAACAAGAAGAACCAAAAGCAGAAGAAAAGGCAGTAGCAGAAGCAGAAGCAGCGGCAGCGAAAAGTGGAGGAGGATGGGGAGGAGGCTGGGGATTCGCCTCCGTTTTCACAGATCTCCAGAAGGCAGCTGAAGAGATCTCTCGCAACGCTGCGGCGGCTGCTCAGACAGCAGCAAAGAGCATTGCCGACATGCAAACCCCTGCCGAAGACTCTGAAGAGTCTTCCAAGGAGGAGGAAGGGGATACTGAAAAGGAAAGCCAAGATGAGCACGAGAAGCAACGAAAGGCTGCTCTGGAGAAATTGGAGAAAGCTAGTGAGGATTCGTTTCTCAGCCAGCATCTGAAGGTACTTGATAGTTCTGTGGAGAACTTTGCTTCAGGAGCATGGCAGGCTTTAGGAAGCGCTTTTAGAGGGGGTACCGATTTAGTTCACAAGCTTGAGCATTCTGCTGTGAACCTAGCAGAATCTATCCAGCGAGATGGTGCTGGTTCTGTTGCACCATCATTATTAGAGACTGGAAAAGCTTTTACCTCAAAGGGAATGCAAGCGCTTGAGCTAGTAGGCAAGGAAACCTTGGATCTTCTGATTACTGAAACTGGTATTGAAGTTGATAAGAATTCTAAGCACTCTGATCAAGAAGGTGAAGAGGATCAGTTATATGAGGAAGTGGGGTTTGATCGATGTTTCTACATATATGGAGGTCCAGAACACTTGGAGGAACTTGAGGCATTGTCCAGTCATTATGCAGTACTATTTAACCGGAGAAAAGGAAAACTATCACCAGAGCAGAAATCTGTTTATGATGGAAGGCTTAAGCTGGTTCAGCAGATTTTCAGTCTGAACTCTGAATTGGATGGAAGTGGGGCAGAGTCGGACAAAGGCAAAAAGAAAGAGACTGGAGCTGATGGAAATAGTGATGAGATGAAGAGCTTACATGATTCAAGTGTCAGCAAAGCTGCTGATATGGCTGCTGGGTTCACGAGTGCCTTAGCAGGACTAGCTGTTAATGATATAATTCAAAGAACTACTGGCAGATTAGAGAGCCTTCACTCGGAGGGGGTTCATAGACTCTCTGAACTTTGCTGTTCTGCGGTGTCTCAACTGCTAATGCTTGGTAAAGCCATCATATCTAATGCAAACAAAGCTCAGGCTGAAGACGTGGATGCTGATTTGGGGAATATTGACTGGCCTGAAGATTCTGTTGAAAAAGCAAAGATAATCAGATCAAAAGCACAAGCAATGACAGGATATGTGGAAGCTGTTTCCAGCAGTTTCATTACAGGCATATCTGATGTTGCTGAAGCGTATGCAGCAGCCATGAAGGGTGCTGCAGAATCCCAAGAAGTGCTTCCGCAGACATCAATGCAGGAAAAAGCAAATTCCTTCTCTGAACATCTCCGTGGCGACCAGACCATAGCTTTGAGCAAAATCCAGGACGGGCTTCACCACTTGTCTTATGTGGTGGTTTCAACCTCTATGCCTGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

513

Amino Acids

54.91

Weight (kDa)

4.73

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 239 - 509 1.4e-138 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 464
AciI CCGC 5 cut(s) 101, 200, 203, 1139, 1400
AclWI GGATC 2 cut(s) 633, 705
AcsI RAATTY 2 cut(s) 664, 1426
AcuI CTGAAG 7 cut(s) 198, 282, 408, 410, 1221, 1260, 1368
AfaI GTAC 3 cut(s) 396, 466, 798
AfeI AGCGCT 2 cut(s) 451, 597
AfiI CCNNNNNNNGG 1 cut(s) 1099
AflII CTTAAG 2 cut(s) 23, 860
AgsI TTSAA 5 cut(s) 653, 990, 1067, 1253, 1518
AjiI CACGTC 1 cut(s) 1207
AjnI CCWGG 1 cut(s) 1479
AjuI GAANNNNNNNTTGG 4 cut(s) 881, 913, 1380, 1412
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 3 cut(s) 554, 935, 1149
AlwI GGATC 2 cut(s) 633, 705
AlwNI CAGNNNCTG 1 cut(s) 388
Aor51HI AGCGCT 2 cut(s) 451, 597
AoxI GGCC 1 cut(s) 1235
ApoI RAATTY 2 cut(s) 664, 1426
ArsI GACNNNNNNTTYG 2 cut(s) 1450, 1482
Asp700I GAANNNNTTC 1 cut(s) 1395
Asp718I GGTACC 1 cut(s) 464
AspLEI GCGC 2 cut(s) 452, 598
AspS9I GGNCC 1 cut(s) 749
AsuHPI GGTGA 3 cut(s) 701, 819, 1484
AvaII GGWCC 1 cut(s) 749
BanI GGYRCC 1 cut(s) 464
BauI CACGAG 2 cut(s) 317, 1027
BbsI GAAGAC 3 cut(s) 261, 261, 1209
Bbv12I GWGCWC 1 cut(s) 318
BccI CCATC 7 cut(s) 117, 521, 556, 845, 896, 947, 1178
BciT130I CCWGG 1 cut(s) 1481
BciVI GTATCC 1 cut(s) 283
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 554, 935, 1149
BfaI CTAG 4 cut(s) 360, 506, 605, 1046
BfmI CTRYAG 2 cut(s) 1377, 1530
BfoI RGCGCY 2 cut(s) 453, 599
BfrI CTTAAG 2 cut(s) 23, 860
BfuI GTATCC 1 cut(s) 283
BglII AGATCT 2 cut(s) 160, 183
BmcAI AGTACT 1 cut(s) 798
Bme1390I CCNGG 1 cut(s) 1481
Bme18I GGWCC 1 cut(s) 749
BmgBI CACGTC 1 cut(s) 1207
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 1 cut(s) 1481
BmsI GCATC 2 cut(s) 394, 1201
BpiI GAAGAC 3 cut(s) 261, 261, 1209
BpmI CTGGAG 3 cut(s) 149, 362, 966
Bpu10I CCTNAGC 2 cut(s) 1036, 1194
BpuEI CTTGAG 3 cut(s) 506, 620, 791
Bsa29I ATCGAT 1 cut(s) 727
BsaJI CCNNGG 3 cut(s) 273, 621, 1447
BsaWI WCCGGW 1 cut(s) 807
Bsc4I CCNNNNNNNGG 1 cut(s) 1099
Bse1I ACTGG 6 cut(s) 568, 649, 783, 949, 1081, 1238
Bse3DI GCAATG 2 cut(s) 228, 1293
BseBI CCWGG 1 cut(s) 1481
BseCI ATCGAT 1 cut(s) 727
BseDI CCNNGG 3 cut(s) 273, 621, 1447
BseGI GGATG 3 cut(s) 128, 907, 1216
BseLI CCNNNNNNNGG 1 cut(s) 1099
BseMI GCAATG 2 cut(s) 228, 1293
BseMII CTCAG 3 cut(s) 224, 391, 1208
BseNI ACTGG 6 cut(s) 568, 649, 783, 949, 1081, 1238
BseRI GAGGAG 3 cut(s) 132, 144, 293
BseYI CCCAGC 2 cut(s) 135, 1019
BshFI GGCC 1 cut(s) 1237
BshNI GGYRCC 1 cut(s) 464
BshVI ATCGAT 1 cut(s) 727
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 1 cut(s) 808
BslI CCNNNNNNNGG 1 cut(s) 1099
BsmAI GTCTC 3 cut(s) 554, 935, 1149
BsmI GAATGC 2 cut(s) 490, 594
BsnI GGCC 1 cut(s) 1237
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
BspACI CCGC 5 cut(s) 101, 200, 203, 1139, 1400
BspANI GGCC 1 cut(s) 1237
BspCNI CTCAG 3 cut(s) 223, 390, 1207
BspDI ATCGAT 1 cut(s) 727
BspLI GGNNCC 1 cut(s) 466
BspMAI CTGCAG 2 cut(s) 1381, 1534
BspPI GGATC 2 cut(s) 633, 705
BspQI GCTCTTC 1 cut(s) 968
BspT107I GGYRCC 1 cut(s) 464
BspTI CTTAAG 2 cut(s) 23, 860
BsrDI GCAATG 2 cut(s) 228, 1293
BsrI ACTGG 6 cut(s) 568, 649, 783, 949, 1081, 1238
BssECI CCNNGG 3 cut(s) 273, 621, 1447
BssMI GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
BssSI CACGAG 2 cut(s) 317, 1027
BssT1I CCWWGG 2 cut(s) 273, 621
Bst2BI CACGAG 2 cut(s) 317, 1027
Bst2UI CCWGG 1 cut(s) 1481
Bst6I CTCTTC 5 cut(s) 3, 174, 258, 687, 968
BstAFI CTTAAG 2 cut(s) 23, 860
BstC8I GCNNGC 4 cut(s) 383, 438, 594, 1530
BstDEI CTNAG 5 cut(s) 210, 377, 669, 1036, 1194
BstDSI CCRYGG 1 cut(s) 1447
BstF5I GGATG 3 cut(s) 128, 907, 1216
BstH2I RGCGCY 2 cut(s) 453, 599
BstHHI GCGC 2 cut(s) 452, 598
BstKTI GATC 7 cut(s) 163, 186, 628, 682, 700, 727, 1274
BstMAI GTCTC 3 cut(s) 554, 935, 1149
BstMBI GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
BstNI CCWGG 1 cut(s) 1481
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 1479
BstSFI CTRYAG 2 cut(s) 1377, 1530
BstV2I GAAGAC 3 cut(s) 261, 261, 1209
BstX2I RGATCY 3 cut(s) 160, 183, 625
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 3 cut(s) 160, 183, 625
Bsu15I ATCGAT 1 cut(s) 727
BsuI GTATCC 1 cut(s) 283
BsuRI GGCC 1 cut(s) 1237
BsuTUI ATCGAT 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 1447
BtrI CACGTC 1 cut(s) 1207
BtsCI GGATG 3 cut(s) 128, 907, 1216
Cac8I GCNNGC 4 cut(s) 383, 438, 594, 1530
CaiI CAGNNNCTG 1 cut(s) 388
CfoI GCGC 2 cut(s) 452, 598
Cfr13I GGNCC 1 cut(s) 749
ClaI ATCGAT 1 cut(s) 727
Csp6I GTAC 3 cut(s) 395, 465, 797
CviAII CATG 4 cut(s) 238, 432, 983, 1366
CviQI GTAC 3 cut(s) 395, 465, 797
DdeI CTNAG 5 cut(s) 210, 377, 669, 1036, 1194
DpnI GATC 7 cut(s) 162, 185, 627, 681, 699, 726, 1273
DpnII GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
Eam1104I CTCTTC 5 cut(s) 3, 174, 258, 687, 968
EarI CTCTTC 5 cut(s) 3, 174, 258, 687, 968
Eco130I CCWWGG 2 cut(s) 273, 621
Eco47I GGWCC 1 cut(s) 749
Eco47III AGCGCT 2 cut(s) 451, 597
Eco57I CTGAAG 7 cut(s) 198, 282, 408, 410, 1221, 1260, 1368
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 1479
EcoT14I CCWWGG 2 cut(s) 273, 621
ErhI CCWWGG 2 cut(s) 273, 621
FaeI CATG 4 cut(s) 241, 435, 986, 1369
FalI AAGNNNNNCTT 4 cut(s) 605, 637, 1380, 1412
FatI CATG 4 cut(s) 237, 431, 982, 1365
FbaI TGATCA 1 cut(s) 679
FokI GGATG 3 cut(s) 135, 914, 1223
FspBI CTAG 4 cut(s) 360, 506, 605, 1046
GlaI GCGC 2 cut(s) 451, 597
GsaI CCCAGC 2 cut(s) 139, 1023
GsuI CTGGAG 3 cut(s) 149, 362, 966
HaeII RGCGCY 2 cut(s) 453, 599
HaeIII GGCC 1 cut(s) 1237
HapII CCGG 1 cut(s) 808
HhaI GCGC 2 cut(s) 452, 598
Hin1II CATG 4 cut(s) 241, 435, 986, 1369
Hin6I GCGC 2 cut(s) 450, 596
HinP1I GCGC 2 cut(s) 450, 596
HindIII AAGCTT 2 cut(s) 482, 570
HpaII CCGG 1 cut(s) 808
HphI GGTGA 3 cut(s) 701, 819, 1484
Hpy166II GTNNAC 3 cut(s) 478, 502, 1026
Hpy188III TCNNGA 6 cut(s) 166, 341, 426, 683, 752, 1027
Hpy8I GTNNAC 3 cut(s) 478, 502, 1026
HpyAV CCTTC 8 cut(s) 163, 278, 385, 680, 849, 1103, 1363, 1441
HpyCH4IV ACGT 1 cut(s) 1206
HpyF3I CTNAG 5 cut(s) 210, 377, 669, 1036, 1194
HpySE526I ACGT 1 cut(s) 1206
Hsp92II CATG 4 cut(s) 241, 435, 986, 1369
HspAI GCGC 2 cut(s) 450, 596
KpnI GGTACC 1 cut(s) 468
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
LguI GCTCTTC 1 cut(s) 968
LmnI GCTCC 2 cut(s) 428, 947
LweI GCATC 2 cut(s) 394, 1201
MaeI CTAG 4 cut(s) 360, 506, 605, 1046
MaeII ACGT 1 cut(s) 1206
MalI GATC 7 cut(s) 162, 185, 627, 681, 699, 726, 1273
MboI GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
MflI RGATCY 3 cut(s) 160, 183, 625
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 5 cut(s) 347, 664, 896, 1062, 1426
MlyI GAGTC 4 cut(s) 251, 275, 926, 1110
MmeI TCCRAC 1 cut(s) 900
MroXI GAANNNNTTC 1 cut(s) 1395
MseI TTAA 5 cut(s) 24, 804, 861, 1053, 1537
MslI CAYNNNNRTG 1 cut(s) 1446
MspA1I CMGCKG 2 cut(s) 101, 176
MspCI CTTAAG 2 cut(s) 23, 860
MspI CCGG 1 cut(s) 808
MspR9I CCNGG 1 cut(s) 1481
Mva1269I GAATGC 2 cut(s) 490, 594
MvaI CCWGG 1 cut(s) 1481
NdeII GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
NlaIII CATG 4 cut(s) 241, 435, 986, 1369
NlaIV GGNNCC 1 cut(s) 466
NspI RCATGY 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 968
PctI GAATGC 2 cut(s) 490, 594
PdmI GAANNNNTTC 1 cut(s) 1395
PfeI GAWTC 6 cut(s) 141, 368, 512, 986, 1244, 1382
PfoI TCCNGGA 1 cut(s) 1479
PleI GAGTC 4 cut(s) 251, 274, 925, 1110
PpsI GAGTC 4 cut(s) 251, 274, 925, 1110
Psp6I CCWGG 1 cut(s) 1479
PspFI CCCAGC 2 cut(s) 135, 1019
PspGI CCWGG 1 cut(s) 1479
PspN4I GGNNCC 1 cut(s) 466
PspPI GGNCC 1 cut(s) 749
PstI CTGCAG 2 cut(s) 1381, 1534
PstNI CAGNNNCTG 1 cut(s) 388
PsuI RGATCY 3 cut(s) 160, 183, 625
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 3 cut(s) 396, 466, 798
RsaNI GTAC 3 cut(s) 395, 465, 797
RseI CAYNNNNRTG 1 cut(s) 1446
SapI GCTCTTC 1 cut(s) 968
SaqAI TTAA 5 cut(s) 24, 804, 861, 1053, 1537
Sau3AI GATC 7 cut(s) 160, 183, 625, 679, 697, 724, 1271
Sau96I GGNCC 1 cut(s) 749
ScaI AGTACT 1 cut(s) 798
SchI GAGTC 4 cut(s) 251, 275, 926, 1110
ScrFI CCNGG 1 cut(s) 1481
SduI GDGCHC 1 cut(s) 318
SfaNI GCATC 2 cut(s) 394, 1201
SfcI CTRYAG 2 cut(s) 1377, 1530
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 1446
SmlI CTYRAG 5 cut(s) 23, 485, 599, 770, 860
SmoI CTYRAG 5 cut(s) 23, 485, 599, 770, 860
Sse9I AATT 5 cut(s) 347, 664, 896, 1062, 1426
SsiI CCGC 5 cut(s) 101, 200, 203, 1139, 1400
SspI AATATT 1 cut(s) 1228
SspMI CTAG 4 cut(s) 360, 506, 605, 1046
StyD4I CCNGG 1 cut(s) 1479
StyI CCWWGG 2 cut(s) 273, 621
TaiI ACGT 1 cut(s) 1209
TaqI TCGA 1 cut(s) 727
TasI AATT 5 cut(s) 347, 664, 896, 1062, 1426
TatI WGTACW 1 cut(s) 796
TauI GCSGC 3 cut(s) 104, 203, 206
TfiI GAWTC 6 cut(s) 141, 368, 512, 986, 1244, 1382
Tru1I TTAA 5 cut(s) 24, 804, 861, 1053, 1537
Tru9I TTAA 5 cut(s) 24, 804, 861, 1053, 1537
TspDTI ATGAA 4 cut(s) 986, 1100, 1312, 1382
TspGWI ACGGA 2 cut(s) 139, 1436
Vha464I CTTAAG 2 cut(s) 23, 860
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 2 cut(s) 664, 1426
XceI RCATGY 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 1395
XspI CTAG 4 cut(s) 360, 506, 605, 1046
ZrmI AGTACT 1 cut(s) 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.