MD05G1073700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008704
Physical Location & Seq
Forward (+)
15497560 .. 15502249
4690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1073700.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGGAGCAAGAAGACAAACAGCCGCTCAAGAAGGACAAAGTTCCTCCACAGGAAGAAGGAGAAGCAACTGAAGCGCAAGCGTTGCCGGCGAAAAGTGGCCGAGGATGGGGAGGTTGGGGCTTTTCTTCCGTCTTCTCAGATCTCCAGAAAGCTGCCGAAGAGATCTCTCGGAATGCGGCTGTGGCTGCTCAGACAGCAGCGAAGAGCCTTGCCGACATGCAAGCTGCTGAGGACTCAGAAGAATCTTCCAAGGACGAGGAAAGGGATTCTGAAAAGGAAAGCCAAGACGGCACTGAGAGCCGAGATGAGCATGAAAAGCAAAGAAAGGCTGCTCTTGATAAATTGGAGAAAGCTAGTCAGGATTCGCTTTTTGGTCAGGGTTTGAAGGTGCTTGATAGTTCTGTGGAGAATTTTGCTACAGGAGCATGGCAAGCATTAGGAAATGCGTTGAAAGGGGGTACCGATCTAGTGCACAAGATTGAGAATTCTGCTGTGAACCTTGGGGGATCTGTCCAGCAAGGTGGGGCTGATGGTTCTACTGCACCATCCTTACTAGAGACTGGAAAAGCTTTTACATCAAGGGGAATGCAAGTGCTTGAGCTTGTAGGAAAGGAAACTTTGGATCTACTGATCACAGAGACTGGTATAGAAGTCAACAAGAATTCTACACTTTCTGAAAAGGAAACCGGAGAGGATCAGTTATATGAGGAAGTGGGATTTGATCGATGCTTCTACATATATGGAGGTCCAGAACAGTTGGAGGAACTGGAGGCTTTGTCCAGCCATAATGCACTATTATTTAGTCGGAGAAAAGGCAAATTGTCATCAGAACAGAAGTTTGTTTATGATGGAAAGCTTAAAGAGGTGCAACAGATTTTCAGTCTGGATATTAAGTTGGATGGAAGTGGTGCAGAGTCGGACAAAGGCAAAAAGAAAGAGACCGGAGATGATGGAAATACCGATGAGATGAAAACTCTTCATGATTCAAGCGTCAGTAAGGCTGCTGATATGGCGGCTGGGTTCACAAGTGCCTTGGCAGGACAAGCTGTTAACGATATAATTCAAAGAACCACTGGAAGATTAGAGAGTCTTCACTCAGAGGGGGTTCATAGACTGTCTGAATTGTGCTGTTCTGCTGTATCTCAACTGCTGATTCTTGGTAAATCCATCATATCTAGTGCAAACAAAACTCAGGTAGAAGATGTGGATGCTGATCTGGAGAATATTGATTGGCCTGAAGATTCTGTTGAGAAAGCAAAGATAATCAGATCAAAAGCACAAGCAATGACAGGATACGTGGAGGCAGTGTCTAGTAGTTTCGTCACAGGTATGTCTGATGTTGCCGAAGCTTATCTAGCAGCCATAAGGGGCGCTTCTGAGGAATCCCTGGAAGAGCTCTCACAGACATCGATCCAAGAAAAAGCAAGTACTTTCTCTAAACATCTCCATGATGACCAGACCACAGCTTTGAGCAAAATCCAGGATGGGCTACACTACTTGTCGTACGTTGTCATTTCGACCTCTATGCCTGCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

513

Amino Acids

55.08

Weight (kDa)

4.67

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 238 - 509 7.7e-136 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 458
AccBSI CCGCTC 1 cut(s) 25
AciI CCGC 3 cut(s) 23, 176, 1013
AclWI GGATC 4 cut(s) 514, 630, 702, 1405
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 3 cut(s) 409, 484, 661
AcuI CTGAAG 2 cut(s) 90, 1257
AfaI GTAC 3 cut(s) 460, 1429, 1505
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 4 cut(s) 385, 451, 987, 1064
AjnI CCWGG 2 cut(s) 1386, 1479
Alw21I GWGCWC 2 cut(s) 474, 1398
Alw26I GTCTC 3 cut(s) 551, 632, 932
Alw44I GTGCAC 1 cut(s) 470
AlwI GGATC 4 cut(s) 514, 630, 702, 1405
AlwNI CAGNNNCTG 1 cut(s) 641
AoxI GGCC 2 cut(s) 97, 1232
ApaLI GTGCAC 1 cut(s) 470
ApeKI GCWGC 8 cut(s) 152, 185, 197, 224, 329, 1001, 1358, 1532
ApoI RAATTY 3 cut(s) 409, 484, 661
ArsI GACNNNNNNTTYG 2 cut(s) 1450, 1482
Asp718I GGTACC 1 cut(s) 458
AspLEI GCGC 2 cut(s) 76, 1373
AspS9I GGNCC 1 cut(s) 746
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 474
BanI GGYRCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 1398
BbsI GAAGAC 3 cut(s) 18, 124, 1082
Bbv12I GWGCWC 2 cut(s) 474, 1398
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 7 cut(s) 139, 172, 209, 211, 316, 988, 1370
BccI CCATC 8 cut(s) 99, 524, 553, 842, 893, 944, 1175, 1478
BceAI ACGGC 1 cut(s) 304
BcgI CGANNNNNNTGC 1 cut(s) 1507
BciT130I CCWGG 2 cut(s) 1388, 1481
BciVI GTATCC 1 cut(s) 1286
BclI TGATCA 1 cut(s) 630
BcoDI GTCTC 3 cut(s) 551, 632, 932
BfaI CTAG 6 cut(s) 354, 467, 554, 1176, 1311, 1355
BfmI CTRYAG 2 cut(s) 417, 1530
BfoI RGCGCY 1 cut(s) 1374
BfuI GTATCC 1 cut(s) 1286
BglI GCCNNNNNGGC 1 cut(s) 288
BglII AGATCT 2 cut(s) 139, 162
BmcAI AGTACT 1 cut(s) 1429
Bme1390I CCNGG 2 cut(s) 1388, 1481
Bme18I GGWCC 1 cut(s) 746
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 2 cut(s) 1388, 1481
BmsI GCATC 2 cut(s) 716, 1198
BpiI GAAGAC 3 cut(s) 18, 124, 1082
BpmI CTGGAG 3 cut(s) 128, 788, 1238
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 11, 617
Bsa29I ATCGAT 2 cut(s) 724, 1409
BsaAI YACGTR 1 cut(s) 1297
BsaBI GATNNNNATC 1 cut(s) 1212
BsaI GGTCTC 1 cut(s) 932
BsaJI CCNNGG 5 cut(s) 100, 249, 499, 1032, 1386
BsaWI WCCGGW 2 cut(s) 686, 941
BsaXI ACNNNNNCTCC 4 cut(s) 761, 791, 1292, 1322
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 4 cut(s) 565, 646, 771, 1078
Bse3DI GCAATG 1 cut(s) 1290
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 1388, 1481
BseCI ATCGAT 2 cut(s) 724, 1409
BseDI CCNNGG 5 cut(s) 100, 249, 499, 1032, 1386
BseGI GGATG 5 cut(s) 110, 545, 904, 1213, 1489
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 8 cut(s) 150, 203, 219, 249, 285, 1110, 1205, 1368
BseNI ACTGG 4 cut(s) 565, 646, 771, 1078
BseSI GKGCMC 1 cut(s) 474
BseXI GCAGC 7 cut(s) 139, 172, 209, 211, 316, 988, 1370
BseYI CCCAGC 1 cut(s) 1016
BsgI GTGCAG 2 cut(s) 525, 930
BshFI GGCC 2 cut(s) 99, 1234
BshNI GGYRCC 1 cut(s) 458
BshVI ATCGAT 2 cut(s) 724, 1409
BsiHKAI GWGCWC 2 cut(s) 474, 1398
BsiSI CCGG 3 cut(s) 86, 687, 942
BsiWI CGTACG 1 cut(s) 1503
BslI CCNNNNNNNGG 1 cut(s) 106
BsmAI GTCTC 3 cut(s) 551, 632, 932
BsmI GAATGC 2 cut(s) 178, 591
BsnI GGCC 2 cut(s) 99, 1234
Bso31I GGTCTC 1 cut(s) 932
Bsp1286I GDGCHC 2 cut(s) 474, 1398
BspACI CCGC 3 cut(s) 23, 176, 1013
BspANI GGCC 2 cut(s) 99, 1234
BspCNI CTCAG 8 cut(s) 149, 202, 220, 248, 286, 1109, 1204, 1369
BspDI ATCGAT 2 cut(s) 724, 1409
BspHI TCATGA 1 cut(s) 979
BspLI GGNNCC 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 1534
BspPI GGATC 4 cut(s) 514, 630, 702, 1405
BspQI GCTCTTC 2 cut(s) 197, 1386
BspT107I GGYRCC 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 932
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 1290
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 4 cut(s) 565, 646, 771, 1078
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 5 cut(s) 100, 249, 499, 1032, 1386
BssT1I CCWWGG 3 cut(s) 249, 499, 1032
Bst2UI CCWGG 2 cut(s) 1388, 1481
Bst4CI ACNGT 2 cut(s) 756, 1116
Bst6I CTCTTC 4 cut(s) 153, 197, 981, 1386
BstAPI GCANNNNNTGC 1 cut(s) 82
BstBAI YACGTR 1 cut(s) 1297
BstC8I GCNNGC 5 cut(s) 78, 87, 222, 432, 1530
BstDEI CTNAG 8 cut(s) 136, 189, 228, 235, 294, 1096, 1191, 1377
BstF5I GGATG 5 cut(s) 110, 545, 904, 1213, 1489
BstH2I RGCGCY 1 cut(s) 1374
BstHHI GCGC 2 cut(s) 76, 1373
BstMAI GTCTC 3 cut(s) 551, 632, 932
BstNI CCWGG 2 cut(s) 1388, 1481
BstNSI RCATGY 1 cut(s) 220
BstSCI CCNGG 2 cut(s) 1386, 1479
BstSFI CTRYAG 2 cut(s) 417, 1530
BstSLI GKGCMC 1 cut(s) 474
BstV1I GCAGC 7 cut(s) 139, 172, 209, 211, 316, 988, 1370
BstV2I GAAGAC 3 cut(s) 18, 124, 1082
BstX2I RGATCY 4 cut(s) 139, 162, 506, 622
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 4 cut(s) 139, 162, 506, 622
Bsu15I ATCGAT 2 cut(s) 724, 1409
BsuI GTATCC 1 cut(s) 1286
BsuRI GGCC 2 cut(s) 99, 1234
BsuTUI ATCGAT 2 cut(s) 724, 1409
BtsCI GGATG 5 cut(s) 110, 545, 904, 1213, 1489
BtsI GCAGTG 1 cut(s) 1311
BtsIMutI CAGTG 3 cut(s) 291, 1071, 1311
Cac8I GCNNGC 5 cut(s) 78, 87, 222, 432, 1530
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 1 cut(s) 979
CfoI GCGC 2 cut(s) 76, 1373
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 746
ClaI ATCGAT 2 cut(s) 724, 1409
CseI GACGC 1 cut(s) 979
Csp6I GTAC 3 cut(s) 459, 1428, 1504
CviAII CATG 6 cut(s) 217, 311, 426, 980, 1448, 1536
CviQI GTAC 3 cut(s) 459, 1428, 1504
DdeI CTNAG 8 cut(s) 136, 189, 228, 235, 294, 1096, 1191, 1377
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 4 cut(s) 153, 197, 981, 1386
EarI CTCTTC 4 cut(s) 153, 197, 981, 1386
Ecl136II GAGCTC 1 cut(s) 1396
Eco130I CCWWGG 3 cut(s) 249, 499, 1032
Eco24I GRGCYC 1 cut(s) 1398
Eco31I GGTCTC 1 cut(s) 932
Eco47I GGWCC 1 cut(s) 746
Eco53kI GAGCTC 1 cut(s) 1396
Eco57I CTGAAG 2 cut(s) 90, 1257
EcoICRI GAGCTC 1 cut(s) 1396
EcoRI GAATTC 2 cut(s) 484, 661
EcoRII CCWGG 2 cut(s) 1386, 1479
EcoT14I CCWWGG 3 cut(s) 249, 499, 1032
EcoT38I GRGCYC 1 cut(s) 1398
ErhI CCWWGG 3 cut(s) 249, 499, 1032
FaeI CATG 6 cut(s) 220, 314, 429, 983, 1451, 1539
FalI AAGNNNNNCTT 2 cut(s) 1357, 1389
FatI CATG 6 cut(s) 216, 310, 425, 979, 1447, 1535
FbaI TGATCA 1 cut(s) 630
FokI GGATG 5 cut(s) 117, 532, 911, 1220, 1496
FriOI GRGCYC 1 cut(s) 1398
FspBI CTAG 6 cut(s) 354, 467, 554, 1176, 1311, 1355
GlaI GCGC 2 cut(s) 75, 1372
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 3 cut(s) 128, 788, 1238
HaeII RGCGCY 1 cut(s) 1374
HaeIII GGCC 2 cut(s) 99, 1234
HapII CCGG 3 cut(s) 86, 687, 942
HgaI GACGC 1 cut(s) 979
HhaI GCGC 2 cut(s) 76, 1373
Hin1II CATG 6 cut(s) 220, 314, 429, 983, 1451, 1539
Hin6I GCGC 2 cut(s) 74, 1371
HinP1I GCGC 2 cut(s) 74, 1371
HincII GTYRAC 2 cut(s) 655, 1051
HindII GTYRAC 2 cut(s) 655, 1051
HindIII AAGCTT 3 cut(s) 567, 854, 1347
HpaI GTTAAC 1 cut(s) 1051
HpaII CCGG 3 cut(s) 86, 687, 942
Hpy166II GTNNAC 5 cut(s) 472, 496, 655, 1023, 1051
Hpy188III TCNNGA 8 cut(s) 28, 145, 335, 359, 749, 884, 980, 1217
Hpy8I GTNNAC 5 cut(s) 472, 496, 655, 1023, 1051
HpyAV CCTTC 3 cut(s) 25, 50, 379
HpyCH4III ACNGT 2 cut(s) 756, 1116
HpyCH4IV ACGT 2 cut(s) 1296, 1506
HpyCH4V TGCA 9 cut(s) 220, 472, 542, 589, 791, 868, 911, 1181, 1532
HpyF3I CTNAG 8 cut(s) 136, 189, 228, 235, 294, 1096, 1191, 1377
HpySE526I ACGT 2 cut(s) 1296, 1506
Hsp92II CATG 6 cut(s) 220, 314, 429, 983, 1451, 1539
HspAI GCGC 2 cut(s) 74, 1371
KpnI GGTACC 1 cut(s) 462
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 630
KspAI GTTAAC 1 cut(s) 1051
LguI GCTCTTC 2 cut(s) 197, 1386
LmnI GCTCC 2 cut(s) 4, 422
Lsp1109I GCAGC 7 cut(s) 139, 172, 209, 211, 316, 988, 1370
LweI GCATC 2 cut(s) 716, 1198
MaeI CTAG 6 cut(s) 354, 467, 554, 1176, 1311, 1355
MaeII ACGT 2 cut(s) 1296, 1506
MaeIII GTNAC 1 cut(s) 1321
MbiI CCGCTC 1 cut(s) 25
MflI RGATCY 4 cut(s) 139, 162, 506, 622
MhlI GDGCHC 2 cut(s) 474, 1398
MluCI AATT 7 cut(s) 341, 409, 484, 661, 818, 1059, 1121
MlyI GAGTC 3 cut(s) 227, 923, 1096
MmeI TCCRAC 4 cut(s) 738, 785, 876, 897
MroNI GCCGGC 1 cut(s) 85
MseI TTAA 3 cut(s) 858, 891, 1050
MslI CAYNNNNRTG 2 cut(s) 1328, 1446
MspI CCGG 3 cut(s) 86, 687, 942
MspR9I CCNGG 2 cut(s) 1388, 1481
Mva1269I GAATGC 2 cut(s) 178, 591
MvaI CCWGG 2 cut(s) 1388, 1481
NaeI GCCGGC 1 cut(s) 87
NgoMIV GCCGGC 1 cut(s) 85
NlaIII CATG 6 cut(s) 220, 314, 429, 983, 1451, 1539
NlaIV GGNNCC 1 cut(s) 460
NmeAIII GCCGAG 2 cut(s) 125, 326
NmuCI GTSAC 1 cut(s) 1321
NspI RCATGY 1 cut(s) 220
PagI TCATGA 1 cut(s) 979
PciSI GCTCTTC 2 cut(s) 197, 1386
PctI GAATGC 2 cut(s) 178, 591
PdiI GCCGGC 1 cut(s) 87
PfeI GAWTC 7 cut(s) 242, 266, 362, 983, 1153, 1241, 1382
Pfl23II CGTACG 1 cut(s) 1503
PfoI TCCNGGA 1 cut(s) 1479
PleI GAGTC 3 cut(s) 227, 922, 1095
PpsI GAGTC 3 cut(s) 227, 922, 1095
Ppu21I YACGTR 1 cut(s) 1297
Psp124BI GAGCTC 1 cut(s) 1398
Psp6I CCWGG 2 cut(s) 1386, 1479
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 2 cut(s) 1386, 1479
PspLI CGTACG 1 cut(s) 1503
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 1 cut(s) 1534
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 4 cut(s) 139, 162, 506, 622
RsaI GTAC 3 cut(s) 460, 1429, 1505
RsaNI GTAC 3 cut(s) 459, 1428, 1504
RseI CAYNNNNRTG 2 cut(s) 1328, 1446
SacI GAGCTC 1 cut(s) 1398
SapI GCTCTTC 2 cut(s) 197, 1386
SaqAI TTAA 3 cut(s) 858, 891, 1050
Sau96I GGNCC 1 cut(s) 746
ScaI AGTACT 1 cut(s) 1429
SchI GAGTC 3 cut(s) 227, 923, 1096
ScrFI CCNGG 2 cut(s) 1388, 1481
SduI GDGCHC 2 cut(s) 474, 1398
SfaNI GCATC 2 cut(s) 716, 1198
SfcI CTRYAG 2 cut(s) 417, 1530
SinI GGWCC 1 cut(s) 746
SmiMI CAYNNNNRTG 2 cut(s) 1328, 1446
SmlI CTYRAG 2 cut(s) 26, 596
SmoI CTYRAG 2 cut(s) 26, 596
Sse9I AATT 7 cut(s) 341, 409, 484, 661, 818, 1059, 1121
SsiI CCGC 3 cut(s) 23, 176, 1013
SspI AATATT 1 cut(s) 1225
SspMI CTAG 6 cut(s) 354, 467, 554, 1176, 1311, 1355
SstI GAGCTC 1 cut(s) 1398
StyD4I CCNGG 2 cut(s) 1386, 1479
StyI CCWWGG 3 cut(s) 249, 499, 1032
TaaI ACNGT 2 cut(s) 756, 1116
TaiI ACGT 2 cut(s) 1299, 1509
TaqI TCGA 3 cut(s) 724, 1409, 1517
TasI AATT 7 cut(s) 341, 409, 484, 661, 818, 1059, 1121
TatI WGTACW 1 cut(s) 1427
TauI GCSGC 3 cut(s) 25, 179, 1016
TfiI GAWTC 7 cut(s) 242, 266, 362, 983, 1153, 1241, 1382
Tru1I TTAA 3 cut(s) 858, 891, 1050
Tru9I TTAA 3 cut(s) 858, 891, 1050
TscAI CASTG 3 cut(s) 298, 1078, 1311
TseFI GTSAC 1 cut(s) 1321
TseI GCWGC 8 cut(s) 152, 185, 197, 224, 329, 1001, 1358, 1532
Tsp45I GTSAC 1 cut(s) 1321
TspDTI ATGAA 4 cut(s) 327, 968, 983, 1097
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 3 cut(s) 298, 1078, 1311
VneI GTGCAC 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 746
XapI RAATTY 3 cut(s) 409, 484, 661
XceI RCATGY 1 cut(s) 220
XspI CTAG 6 cut(s) 354, 467, 554, 1176, 1311, 1355
ZrmI AGTACT 1 cut(s) 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.