AT2G16800

high-affinity nickel-transport family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009309
Physical Location & Seq
Forward (+)
7285698 .. 7287393
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G16800.1

Sequence Viewer

Length: 1119 bp
ATGGATAGGCTTTTGCAACCTCCTTCTTCTCACTCAATTGCTCCCTCCAAATTCCAATCAAGACCATCTCCCTTATTACTCCACCGTCTCCACTCAACGAATCTTAGTACTTTTCCCTCCTCTCGCCGTCTCGAGTCACGCCGAATCAGCTCCATTTCCTGCTTCTTCCGTCAGAATCCATCGCCGGATACATCTCCAGGATTGAATCAGAGTAGTAATTTCTTGATTGCTTCTTCTCAAACCGATGCGTCGAAACCTAATCCAGGATTTATCCAACGGATCGTGAGCAGTTTTGAGCAACGCAAGACCATATCGGCAGGAATGGTTATATTAGTCTCTGCTATAGCTGCGCTTTTGCTTAATCCCATTCTTGTGCCGCCTGCTTTCGCTAGCTTTCAAACGGCTACTAATTCTGGTGGAGCTGCTGTGGTTGGTGGAAAACTCCTCAGGACTGAAGTATTGACAAGTGCTTGGACTGGTTTCTTTGCTGGTTGCTTACACACGTTATCTGGACCTGATCACCTTGCTGCTTTGGCTCCACTTTCGATTGGACGGACGAGAATGGAGAGTGCTGCGGTTGGAGCTCTTTGGGGATGTGGTCATGACGCTGGTCAGCTCATCTTTGGTTTATTGTTTCTGCTTCTAAAAGATAGGCTCCACATTGAAGTTATAAGAACTTGGGGTACAAGAGTTGTTGGTTTGACTCTCCTTGTGATTGGTGCTATGGGAATCAAAGAAGCTTCTGAGATGCCGGAACCATGTGTTGTTACTTTGGAGAATGGGGAAACAGATGAGAAAAGCTTAAAGAAGAAAAAGATAGGGTTTGCGACATTTGCGACTGGGATTGTTCATGGCCTGCAACCGGATGCTCTGATGATGGTATTGCCTGCATTGGCTCTTCCTTCTCGGATAGCCGGTGCTTCGTTTCTCATCATGTTCTTGATTGGGACGGTGATTGCGATGGGAAGCTACACAGTTTTTATTGGGTCGTGTAGCGAAGCGTTGAAAGAGAAAGTTCCTAGGATCACTGAGAAACTAACGTGGGCATCTTCTCTTGTGGCCATTGGACTTGGATTGGCAATTATTGTCAGCCAGTTCTTTGGATTCAGCCTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DsbD_2

Protein Analysis

372

Amino Acids

39.69

Weight (kDa)

9.64

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DsbD_2 PF13386 206 - 344 1.3e-06 Cytochrome C biogenesis protein transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AciI CCGC 2 cut(s) 377, 575
AclWI GGATC 2 cut(s) 287, 1031
AcoI YGGCCR 1 cut(s) 1059
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 2 cut(s) 109, 685
AfiI CCNNNNNNNGG 2 cut(s) 263, 862
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 4 cut(s) 205, 398, 665, 1006
AjnI CCWGG 2 cut(s) 196, 262
AluBI AGCT 9 cut(s) 150, 347, 393, 422, 584, 616, 740, 801, 969
AluI AGCT 9 cut(s) 150, 347, 393, 422, 584, 616, 740, 801, 969
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 3 cut(s) 92, 134, 340
AlwI GGATC 2 cut(s) 287, 1031
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 853, 1059
ApeKI GCWGC 4 cut(s) 347, 422, 527, 572
ApoI RAATTY 1 cut(s) 50
AspA2I CCTAGG 1 cut(s) 1019
AspLEI GCGC 1 cut(s) 352
AspS9I GGNCC 1 cut(s) 512
AsuHPI GGTGA 2 cut(s) 512, 964
AsuNHI GCTAGC 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 512
AvrII CCTAGG 1 cut(s) 1019
AxyI CCTNAGG 1 cut(s) 446
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BalI TGGCCA 1 cut(s) 1061
BanII GRGCYC 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 4 cut(s) 334, 409, 514, 559
BccI CCATC 4 cut(s) 73, 187, 871, 955
BceAI ACGGC 2 cut(s) 111, 417
BciT130I CCWGG 2 cut(s) 198, 264
BciVI GTATCC 1 cut(s) 181
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 3 cut(s) 92, 134, 340
BfaI CTAG 2 cut(s) 390, 1020
BfmI CTRYAG 1 cut(s) 342
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 5 cut(s) 348, 377, 423, 528, 573
BlnI CCTAGG 1 cut(s) 1019
BlsI GCNGC 5 cut(s) 349, 378, 424, 529, 574
BmcAI AGTACT 1 cut(s) 109
Bme1390I CCNGG 2 cut(s) 198, 264
Bme18I GGWCC 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 512
BmiI GGNNCC 3 cut(s) 537, 656, 756
BmrFI CCNGG 2 cut(s) 198, 264
BmrI ACTGGG 1 cut(s) 849
BmsI GCATC 4 cut(s) 235, 738, 856, 1055
BmtI GCTAGC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 849
BoxI GACNNNNGTC 1 cut(s) 609
BpmI CTGGAG 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 1019
BsaWI WCCGGW 1 cut(s) 862
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 862
Bse118I RCCGGY 1 cut(s) 914
Bse1I ACTGG 3 cut(s) 481, 844, 1093
Bse21I CCTNAGG 1 cut(s) 446
BseBI CCWGG 2 cut(s) 198, 264
BseDI CCNNGG 1 cut(s) 1019
BseGI GGATG 2 cut(s) 599, 871
BseLI CCNNNNNNNGG 2 cut(s) 263, 862
BseMII CTCAG 3 cut(s) 460, 735, 1020
BseNI ACTGG 3 cut(s) 481, 844, 1093
BseRI GAGGAG 2 cut(s) 109, 434
BseXI GCAGC 4 cut(s) 334, 409, 514, 559
BshFI GGCC 2 cut(s) 855, 1061
BsiHKAI GWGCWC 1 cut(s) 586
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 4 cut(s) 185, 752, 863, 915
BslFI GGGAC 1 cut(s) 961
BslI CCNNNNNNNGG 2 cut(s) 263, 862
BsmAI GTCTC 3 cut(s) 92, 134, 340
BsmBI CGTCTC 2 cut(s) 92, 134
BsmFI GGGAC 1 cut(s) 961
BsnI GGCC 2 cut(s) 855, 1061
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 3 cut(s) 279, 517, 1023
BspACI CCGC 2 cut(s) 377, 575
BspANI GGCC 2 cut(s) 855, 1061
BspCNI CTCAG 3 cut(s) 459, 736, 1021
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 3 cut(s) 537, 656, 756
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 2 cut(s) 287, 1031
BspQI GCTCTTC 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 914
BsrI ACTGG 3 cut(s) 481, 844, 1093
BssAI RCCGGY 1 cut(s) 914
BssECI CCNNGG 1 cut(s) 1019
BssMI GATC 3 cut(s) 279, 517, 1023
BssT1I CCWWGG 1 cut(s) 1019
Bst2UI CCWGG 2 cut(s) 198, 264
Bst4CI ACNGT 3 cut(s) 86, 952, 976
Bst6I CTCTTC 1 cut(s) 903
BstC8I GCNNGC 4 cut(s) 381, 391, 857, 888
BstDEI CTNAG 4 cut(s) 104, 446, 744, 1029
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 599, 871
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 3 cut(s) 282, 520, 1026
BstMAI GTCTC 3 cut(s) 92, 134, 340
BstMBI GATC 3 cut(s) 279, 517, 1023
BstMWI GCNNNNNNNGC 5 cut(s) 147, 347, 533, 581, 833
BstNI CCWGG 2 cut(s) 198, 264
BstPAI GACNNNNGTC 1 cut(s) 609
BstSCI CCNGG 2 cut(s) 196, 262
BstSFI CTRYAG 1 cut(s) 342
BstV1I GCAGC 4 cut(s) 334, 409, 514, 559
BstXI CCANNNNNNTGG 1 cut(s) 1100
Bsu36I CCTNAGG 1 cut(s) 446
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 855, 1061
BtgZI GCGATG 2 cut(s) 165, 974
BtsCI GGATG 2 cut(s) 599, 871
BtsIMutI CAGTG 1 cut(s) 1026
Cac8I GCNNGC 4 cut(s) 381, 391, 857, 888
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 352
Cfr10I RCCGGY 1 cut(s) 914
Cfr13I GGNCC 1 cut(s) 512
CseI GACGC 2 cut(s) 237, 614
Csp6I GTAC 2 cut(s) 108, 684
CviAII CATG 4 cut(s) 602, 759, 851, 934
CviQI GTAC 2 cut(s) 108, 684
DdeI CTNAG 4 cut(s) 104, 446, 744, 1029
DpnI GATC 3 cut(s) 281, 519, 1025
DpnII GATC 3 cut(s) 279, 517, 1023
EaeI YGGCCR 1 cut(s) 1059
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
Ecl136II GAGCTC 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 1019
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 512
Eco53kI GAGCTC 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 474
Eco81I CCTNAGG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 196, 262
EcoT14I CCWWGG 1 cut(s) 1019
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 1019
Esp3I CGTCTC 2 cut(s) 92, 134
FaeI CATG 4 cut(s) 605, 762, 854, 937
FaiI YATR 9 cut(s) 311, 329, 344, 603, 671, 725, 760, 852, 935
FaqI GGGAC 1 cut(s) 961
FatI CATG 4 cut(s) 601, 758, 850, 933
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 5 cut(s) 348, 377, 423, 528, 573
FokI GGATG 2 cut(s) 606, 878
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 5 cut(s) 348, 377, 423, 528, 573
FspBI CTAG 2 cut(s) 390, 1020
GlaI GCGC 1 cut(s) 351
GluI GCNGC 5 cut(s) 348, 377, 423, 528, 573
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 2 cut(s) 855, 1061
HapII CCGG 4 cut(s) 185, 752, 863, 915
HgaI GACGC 2 cut(s) 237, 614
HhaI GCGC 1 cut(s) 352
Hin1II CATG 4 cut(s) 605, 762, 854, 937
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HindIII AAGCTT 2 cut(s) 738, 799
HinfI GANTC 8 cut(s) 100, 134, 144, 175, 205, 703, 729, 1104
HpaII CCGG 4 cut(s) 185, 752, 863, 915
HphI GGTGA 2 cut(s) 512, 964
Hpy188I TCNGA 5 cut(s) 174, 210, 745, 873, 909
Hpy188III TCNNGA 8 cut(s) 60, 131, 223, 283, 448, 510, 602, 940
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 2 cut(s) 33, 912
HpyCH4III ACNGT 3 cut(s) 86, 952, 976
HpyCH4IV ACGT 2 cut(s) 503, 1040
HpyCH4V TGCA 3 cut(s) 16, 859, 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 147, 347, 533, 581, 833
HpyF3I CTNAG 4 cut(s) 104, 446, 744, 1029
HpySE526I ACGT 2 cut(s) 503, 1040
Hsp92II CATG 4 cut(s) 605, 762, 854, 937
HspAI GCGC 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 3 cut(s) 279, 517, 1023
LguI GCTCTTC 1 cut(s) 903
LmnI GCTCC 6 cut(s) 46, 155, 419, 541, 581, 660
Lsp1109I GCAGC 4 cut(s) 334, 409, 514, 559
LweI GCATC 4 cut(s) 235, 738, 856, 1055
MaeI CTAG 2 cut(s) 390, 1020
MaeII ACGT 2 cut(s) 503, 1040
MaeIII GTNAC 2 cut(s) 135, 766
MalI GATC 3 cut(s) 281, 519, 1025
MboI GATC 3 cut(s) 279, 517, 1023
MboII GAAGA 6 cut(s) 18, 157, 225, 820, 890, 1041
MfeI CAATTG 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 586
MlsI TGGCCA 1 cut(s) 1061
MluCI AATT 5 cut(s) 36, 50, 217, 409, 1080
MluNI TGGCCA 1 cut(s) 1061
MlyI GAGTC 2 cut(s) 143, 697
MmeI TCCRAC 2 cut(s) 298, 559
MnlI CCTC 5 cut(s) 30, 55, 127, 130, 455
Mox20I TGGCCA 1 cut(s) 1061
MscI TGGCCA 1 cut(s) 1061
MseI TTAA 2 cut(s) 360, 803
MslI CAYNNNNRTG 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 1061
MspI CCGG 4 cut(s) 185, 752, 863, 915
MspR9I CCNGG 2 cut(s) 198, 264
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 2 cut(s) 198, 264
MwoI GCNNNNNNNGC 5 cut(s) 147, 347, 533, 581, 833
NdeII GATC 3 cut(s) 279, 517, 1023
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 4 cut(s) 605, 762, 854, 937
NlaIV GGNNCC 3 cut(s) 537, 656, 756
NmuCI GTSAC 1 cut(s) 135
PaeR7I CTCGAG 1 cut(s) 131
PagI TCATGA 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 1 cut(s) 956
PfeI GAWTC 6 cut(s) 100, 144, 175, 205, 729, 1104
PfoI TCCNGGA 2 cut(s) 196, 262
PkrI GCNGC 5 cut(s) 349, 378, 424, 529, 574
PleI GAGTC 2 cut(s) 142, 697
PpsI GAGTC 2 cut(s) 142, 697
PshAI GACNNNNGTC 1 cut(s) 609
PsiI TTATAA 1 cut(s) 671
Psp124BI GAGCTC 1 cut(s) 586
Psp6I CCWGG 2 cut(s) 196, 262
PspGI CCWGG 2 cut(s) 196, 262
PspN4I GGNNCC 3 cut(s) 537, 656, 756
PspPI GGNCC 1 cut(s) 512
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
RsaI GTAC 2 cut(s) 109, 685
RsaNI GTAC 2 cut(s) 108, 684
RseI CAYNNNNRTG 1 cut(s) 371
SacI GAGCTC 1 cut(s) 586
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 2 cut(s) 360, 803
SatI GCNGC 5 cut(s) 348, 377, 423, 528, 573
Sau3AI GATC 3 cut(s) 279, 517, 1023
Sau96I GGNCC 1 cut(s) 512
ScaI AGTACT 1 cut(s) 109
SchI GAGTC 2 cut(s) 143, 697
ScrFI CCNGG 2 cut(s) 198, 264
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 4 cut(s) 235, 738, 856, 1055
SfcI CTRYAG 1 cut(s) 342
Sfr274I CTCGAG 1 cut(s) 131
SinI GGWCC 1 cut(s) 512
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 5 cut(s) 36, 50, 217, 409, 1080
SsiI CCGC 2 cut(s) 377, 575
SspMI CTAG 2 cut(s) 390, 1020
SstI GAGCTC 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 196, 262
StyI CCWWGG 1 cut(s) 1019
TaaI ACNGT 3 cut(s) 86, 952, 976
TaiI ACGT 2 cut(s) 506, 1043
TaqI TCGA 3 cut(s) 132, 251, 545
TasI AATT 5 cut(s) 36, 50, 217, 409, 1080
TatI WGTACW 1 cut(s) 107
TauI GCSGC 1 cut(s) 379
TfiI GAWTC 6 cut(s) 100, 144, 175, 205, 729, 1104
Tru1I TTAA 2 cut(s) 360, 803
Tru9I TTAA 2 cut(s) 360, 803
TscAI CASTG 1 cut(s) 1033
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 4 cut(s) 347, 422, 527, 572
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 1 cut(s) 839
TspGWI ACGGA 3 cut(s) 158, 292, 568
TspRI CASTG 1 cut(s) 1033
VpaK11BI GGWCC 1 cut(s) 512
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 1 cut(s) 50
XhoI CTCGAG 1 cut(s) 131
XmaJI CCTAGG 1 cut(s) 1019
XspI CTAG 2 cut(s) 390, 1020
ZrmI AGTACT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.