Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009309

Physical Location & Seq

Genomic Coordinates Forward (+)

24478721 .. 24481899

Gene Structure 3179 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1122200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGGAAAGGCTTCTGTCCTCTTCACCCCCAACCCCTTTGAAACTCCATCTAAAACCCTCTCCTTTCCTCAGTCGACTCGCCCGTATTGACTCGGCCAAACTCGGTTTTCCCGCTTCACAACTGCCCAATCTCAGACGGGTCCGCTTGGTTTCCTGCAAACATCAAAACCCATCTCCATTTTCTTCTGGGTCTCCTTCATCGGCGCCGTCGAACATTCTGCCGTCTCTCTCGTCGAACCGCGCCGGGTCGCCAAATGGGTCGGAGCCAAAATCCCAGATTCTCAATCAGATCGCAACCAGGGATTCTAAGCTGCGCAAGGCCATCACCACTGGAGCATTTGTTGCGCTTTCTGCACTTGTTTTGATCTTAATACAACCAGCTTTTGCACCAGCGGCTTTTGCATCCTTTCCAACTGCAACCAACACTGGTAGCCCAGCGGCTGCTATTGGGGGAAGACTGATCCAGACAGAGTTGCTTAGTAGTGCATGGACTGGTTTCTTTGCTGGTTGCTTACACACATTATCGGGGCCAGACCACCTAGCAGCTTTGGCTCCACTCTCAATTGGACGTACCCGCATAGAAAGTGCTCTAGTCGGAGCCCTCTGGGGATGTGGCCATGACGCTGGCCAGGTTATCTTTGGCTTGATATTTCTTTTACTAAAGGATAGGCTTCATATTGAGATTATCCGAACTTGGGGCACAAGAGTTGTGGGGCTTACCCTACTAGTTATTGGTGGTATGGGTATTAAGGAAGCTTCAGAAATCCCTACTCCTTGTGTTGCCTTAGAGAATGGCGAGTGTGATGTTAGTGTATATGAGTCTCTAGATAACCCTACAGTCGGAAAGAAGAAGATTGGTTTTGCTACTTTTGCCACTGGAATTGTCCACGGGCTGCAGCCAGATGCATTGATGATGGTCTTGCCAGCCCTCGCTTTGCCATCTCGAGTAGCTGGCGCTGCATTCCTGGTTATGTTTTTAGTTGGGACCGTAGTTGCAATGGGAAGCTATACTGTCTTTCTAGGTTCATGTAGTCGGGCATTAAAGGATCGAGTTCCCAGAATTACAGAGAAACTGACATGGGCTTCTTCCCTGGTTGCAATTGCTCTTGGGTTCGCCATCATCATCAGCCAGTTCTTCGGGTATAGCCTGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DsbD_2

Protein Analysis

385

Amino Acids

40.44

Weight (kDa)

9.78

Isoelectric Point (pI)

43.34

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009309)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16800
fragaria_vesca	FvH4_5g30360
malus_domestica	MD05G1122200.v1.1 MD10G1124900.v1.1
prunus_persica	Prupe.7G263700_v2.0.a1 Prupe.8G165900_v2.0.a1
pyrus_communis	pycom05g11620 pycom10g10720
rosa_chinensis	RchiOBHm_Chr7g0224771
rosa_laevigata	RLG00000001855 RLG00000034639
rosa_multiflora	Rmu_co8174708.1_g000001 Rmu_sc0000144.1_g000012 Rmu_sc0010368.1_g000001
rosa_roxburghii	Rroxscaffold_3G00234400
rosa_rugosa	Rorug05G0250800 Rorug07G0225600
rosa_samantha	Rh7AG377500 Rh7BG361700 Rh7CG396300 Rh7DG371400
rosa_wichuraiana	Rw7G031350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	314
AccB1I	GGYRCC	1 cut(s)	202
AccI	GTMKAC	1 cut(s)	73
AccII	CGCG	1 cut(s)	240
AciI	CCGC	6 cut(s)	111, 142, 238, 392, 437, 574
AclWI	GGATC	2 cut(s)	454, 1053
AcoI	YGGCCR	3 cut(s)	93, 613, 625
AcuI	CTGAAG	1 cut(s)	741
AcyI	GRCGYC	1 cut(s)	203
AfaI	GTAC	1 cut(s)	571
AfiI	CCNNNNNNNGG	2 cut(s)	694, 839
AgsI	TTSAA	1 cut(s)	40
AhlI	ACTAGT	1 cut(s)	724
AjnI	CCWGG	4 cut(s)	296, 627, 963, 1089
AluBI	AGCT	6 cut(s)	310, 380, 545, 755, 950, 1005
AluI	AGCT	6 cut(s)	310, 380, 545, 755, 950, 1005
Alw21I	GWGCWC	1 cut(s)	589
Alw26I	GTCTC	3 cut(s)	195, 228, 825
AlwI	GGATC	2 cut(s)	454, 1053
AlwNI	CAGNNNCTG	1 cut(s)	440
Ama87I	CYCGRG	1 cut(s)	942
AoxI	GGCC	5 cut(s)	93, 318, 527, 613, 625
ApeKI	GCWGC	6 cut(s)	310, 440, 542, 892, 895, 956
Asp700I	GAANNNNTTC	1 cut(s)	9
AspLEI	GCGC	5 cut(s)	205, 242, 315, 346, 956
AspS9I	GGNCC	3 cut(s)	139, 527, 984
AsuC2I	CCSGG	1 cut(s)	244
AsuHPI	GGTGA	2 cut(s)	15, 316
AvaI	CYCGRG	1 cut(s)	942
AvaII	GGWCC	2 cut(s)	139, 984
BaeGI	GKGCMC	1 cut(s)	701
BalI	TGGCCA	2 cut(s)	615, 627
BanI	GGYRCC	1 cut(s)	202
BanII	GRGCYC	1 cut(s)	601
BbsI	GAAGAC	1 cut(s)	460
Bbv12I	GWGCWC	1 cut(s)	589
BbvI	GCAGC	6 cut(s)	297, 427, 554, 879, 907, 943
BccI	CCATC	6 cut(s)	54, 178, 329, 907, 946, 1124
BceAI	ACGGC	2 cut(s)	190, 205
BcgI	CGANNNNNNTGC	2 cut(s)	199, 233
BciT130I	CCWGG	4 cut(s)	298, 629, 965, 1091
BcnI	CCSGG	1 cut(s)	244
BcoDI	GTCTC	3 cut(s)	195, 228, 825
BcuI	ACTAGT	1 cut(s)	724
BfaI	CTAG	5 cut(s)	539, 590, 725, 824, 1019
BfmI	CTRYAG	2 cut(s)	834, 893
BfoI	RGCGCY	2 cut(s)	206, 957
BisI	GCNGC	8 cut(s)	311, 393, 438, 441, 543, 893, 896, 957
BlsI	GCNGC	8 cut(s)	312, 394, 439, 442, 544, 894, 897, 958
Bme1390I	CCNGG	5 cut(s)	244, 298, 629, 965, 1091
Bme18I	GGWCC	2 cut(s)	139, 984
BmeT110I	CYCGRG	1 cut(s)	942
BmgT120I	GGNCC	3 cut(s)	139, 527, 984
BmiI	GGNNCC	7 cut(s)	140, 204, 264, 528, 552, 598, 985
BmrFI	CCNGG	5 cut(s)	244, 298, 629, 965, 1091
BmsI	GCATC	2 cut(s)	410, 892
BpiI	GAAGAC	1 cut(s)	460
BpmI	CTGGAG	1 cut(s)	351
BpuMI	CCSGG	1 cut(s)	244
BsaHI	GRCGYC	1 cut(s)	203
BsaI	GGTCTC	1 cut(s)	195
BsaJI	CCNNGG	3 cut(s)	297, 886, 1089
BsaXI	ACNNNNNCTCC	2 cut(s)	324, 354
Bsc4I	CCNNNNNNNGG	2 cut(s)	694, 839
Bse1I	ACTGG	5 cut(s)	334, 430, 496, 880, 1129
Bse3DI	GCAATG	1 cut(s)	1002
BseBI	CCWGG	4 cut(s)	298, 629, 965, 1091
BseDI	CCNNGG	3 cut(s)	297, 886, 1089
BseGI	GGATG	2 cut(s)	401, 614
BseLI	CCNNNNNNNGG	2 cut(s)	694, 839
BseMI	GCAATG	1 cut(s)	1002
BseMII	CTCAG	2 cut(s)	82, 145
BseNI	ACTGG	5 cut(s)	334, 430, 496, 880, 1129
BseSI	GKGCMC	1 cut(s)	701
BseXI	GCAGC	6 cut(s)	297, 427, 554, 879, 907, 943
BseYI	CCCAGC	1 cut(s)	433
BsgI	GTGCAG	1 cut(s)	336
Bsh1236I	CGCG	1 cut(s)	240
BshFI	GGCC	5 cut(s)	95, 320, 529, 615, 627
BshNI	GGYRCC	1 cut(s)	202
BsiHKAI	GWGCWC	1 cut(s)	589
BsiHKCI	CYCGRG	1 cut(s)	942
BsiSI	CCGG	1 cut(s)	243
BslFI	GGGAC	1 cut(s)	997
BslI	CCNNNNNNNGG	2 cut(s)	694, 839
BsmAI	GTCTC	3 cut(s)	195, 228, 825
BsmBI	CGTCTC	1 cut(s)	228
BsmFI	GGGAC	1 cut(s)	997
BsmI	GAATGC	1 cut(s)	959
BsnI	GGCC	5 cut(s)	95, 320, 529, 615, 627
Bso31I	GGTCTC	1 cut(s)	195
BsoBI	CYCGRG	1 cut(s)	942
Bsp1286I	GDGCHC	3 cut(s)	589, 601, 701
Bsp143I	GATC	4 cut(s)	288, 363, 459, 1045
BspACI	CCGC	6 cut(s)	111, 142, 238, 392, 437, 574
BspANI	GGCC	5 cut(s)	95, 320, 529, 615, 627
BspCNI	CTCAG	2 cut(s)	81, 144
BspFNI	CGCG	1 cut(s)	240
BspLI	GGNNCC	7 cut(s)	140, 204, 264, 528, 552, 598, 985
BspMAI	CTGCAG	1 cut(s)	897
BspPI	GGATC	2 cut(s)	454, 1053
BspT107I	GGYRCC	1 cut(s)	202
BspTNI	GGTCTC	1 cut(s)	195
BsrDI	GCAATG	1 cut(s)	1002
BsrI	ACTGG	5 cut(s)	334, 430, 496, 880, 1129
BssECI	CCNNGG	3 cut(s)	297, 886, 1089
BssMI	GATC	4 cut(s)	288, 363, 459, 1045
BssNI	GRCGYC	1 cut(s)	203
Bst2UI	CCWGG	4 cut(s)	298, 629, 965, 1091
Bst4CI	ACNGT	3 cut(s)	838, 988, 1012
Bst6I	CTCTTC	1 cut(s)	25
BstACI	GRCGYC	1 cut(s)	203
BstAPI	GCANNNNNTGC	1 cut(s)	341
BstC8I	GCNNGC	3 cut(s)	625, 924, 952
BstDEI	CTNAG	5 cut(s)	68, 131, 306, 476, 784
BstDSI	CCRYGG	1 cut(s)	886
BstF5I	GGATG	2 cut(s)	401, 614
BstFNI	CGCG	1 cut(s)	240
BstH2I	RGCGCY	2 cut(s)	206, 957
BstHHI	GCGC	5 cut(s)	205, 242, 315, 346, 956
BstKTI	GATC	4 cut(s)	291, 366, 462, 1048
BstMAI	GTCTC	3 cut(s)	195, 228, 825
BstMBI	GATC	4 cut(s)	288, 363, 459, 1045
BstMWI	GCNNNNNNNGC	7 cut(s)	341, 350, 392, 398, 548, 869, 956
BstNI	CCWGG	4 cut(s)	298, 629, 965, 1091
BstSCI	CCNGG	5 cut(s)	242, 296, 627, 963, 1089
BstSFI	CTRYAG	2 cut(s)	834, 893
BstSLI	GKGCMC	1 cut(s)	701
BstUI	CGCG	1 cut(s)	240
BstV1I	GCAGC	6 cut(s)	297, 427, 554, 879, 907, 943
BstV2I	GAAGAC	1 cut(s)	460
BstXI	CCANNNNNNTGG	1 cut(s)	623
BsuRI	GGCC	5 cut(s)	95, 320, 529, 615, 627
BtgI	CCRYGG	1 cut(s)	886
BtsCI	GGATG	2 cut(s)	401, 614
BtsIMutI	CAGTG	3 cut(s)	327, 423, 873
Cac8I	GCNNGC	3 cut(s)	625, 924, 952
CaiI	CAGNNNCTG	1 cut(s)	440
CfoI	GCGC	5 cut(s)	205, 242, 315, 346, 956
Cfr13I	GGNCC	3 cut(s)	139, 527, 984
CseI	GACGC	1 cut(s)	629
Csp6I	GTAC	1 cut(s)	570
CspCI	CAANNNNNGTGG	4 cut(s)	690, 725, 862, 897
CviAII	CATG	4 cut(s)	486, 617, 1026, 1077
CviQI	GTAC	1 cut(s)	570
DdeI	CTNAG	5 cut(s)	68, 131, 306, 476, 784
DinI	GGCGCC	1 cut(s)	204
DpnI	GATC	4 cut(s)	290, 365, 461, 1047
DpnII	GATC	4 cut(s)	288, 363, 459, 1045
EaeI	YGGCCR	3 cut(s)	93, 613, 625
Eam1104I	CTCTTC	1 cut(s)	25
EarI	CTCTTC	1 cut(s)	25
Eco24I	GRGCYC	1 cut(s)	601
Eco31I	GGTCTC	1 cut(s)	195
Eco47I	GGWCC	2 cut(s)	139, 984
Eco57I	CTGAAG	1 cut(s)	741
Eco88I	CYCGRG	1 cut(s)	942
EcoRII	CCWGG	4 cut(s)	296, 627, 963, 1089
EcoT22I	ATGCAT	1 cut(s)	907
EcoT38I	GRGCYC	1 cut(s)	601
EgeI	GGCGCC	1 cut(s)	204
EheI	GGCGCC	1 cut(s)	204
Esp3I	CGTCTC	1 cut(s)	228
FaeI	CATG	4 cut(s)	489, 620, 1029, 1080
FaqI	GGGAC	1 cut(s)	997
FatI	CATG	4 cut(s)	485, 616, 1025, 1076
FauI	CCCGC	2 cut(s)	118, 581
FblI	GTMKAC	1 cut(s)	73
Fnu4HI	GCNGC	8 cut(s)	311, 393, 438, 441, 543, 893, 896, 957
FokI	GGATG	2 cut(s)	388, 621
FriOI	GRGCYC	1 cut(s)	601
Fsp4HI	GCNGC	8 cut(s)	311, 393, 438, 441, 543, 893, 896, 957
FspBI	CTAG	5 cut(s)	539, 590, 725, 824, 1019
FspI	TGCGCA	1 cut(s)	314
GlaI	GCGC	5 cut(s)	204, 241, 314, 345, 955
GluI	GCNGC	8 cut(s)	311, 393, 438, 441, 543, 893, 896, 957
GsaI	CCCAGC	1 cut(s)	437
GsuI	CTGGAG	1 cut(s)	351
HaeII	RGCGCY	2 cut(s)	206, 957
HaeIII	GGCC	5 cut(s)	95, 320, 529, 615, 627
HapII	CCGG	1 cut(s)	243
HgaI	GACGC	1 cut(s)	629
HhaI	GCGC	5 cut(s)	205, 242, 315, 346, 956
Hin1I	GRCGYC	1 cut(s)	203
Hin1II	CATG	4 cut(s)	489, 620, 1029, 1080
Hin6I	GCGC	5 cut(s)	203, 240, 313, 344, 954
HinP1I	GCGC	5 cut(s)	203, 240, 313, 344, 954
HincII	GTYRAC	1 cut(s)	74
HindII	GTYRAC	1 cut(s)	74
HindIII	AAGCTT	1 cut(s)	753
HinfI	GANTC	5 cut(s)	75, 89, 277, 302, 818
HpaII	CCGG	1 cut(s)	243
HphI	GGTGA	2 cut(s)	15, 316
Hpy166II	GTNNAC	2 cut(s)	74, 886
Hpy188I	TCNGA	7 cut(s)	134, 262, 288, 596, 689, 760, 842
Hpy188III	TCNNGA	3 cut(s)	463, 824, 942
Hpy8I	GTNNAC	2 cut(s)	74, 886
Hpy99I	CGWCG	2 cut(s)	211, 235
HpyAV	CCTTC	1 cut(s)	204
HpyCH4III	ACNGT	3 cut(s)	838, 988, 1012
HpyCH4IV	ACGT	1 cut(s)	568
HpyF10VI	GCNNNNNNNGC	7 cut(s)	341, 350, 392, 398, 548, 869, 956
HpyF3I	CTNAG	5 cut(s)	68, 131, 306, 476, 784
HpySE526I	ACGT	1 cut(s)	568
Hsp92I	GRCGYC	1 cut(s)	203
Hsp92II	CATG	4 cut(s)	489, 620, 1029, 1080
HspAI	GCGC	5 cut(s)	203, 240, 313, 344, 954
KasI	GGCGCC	1 cut(s)	202
Kzo9I	GATC	4 cut(s)	288, 363, 459, 1045
LmnI	GCTCC	4 cut(s)	262, 332, 556, 596
Lsp1109I	GCAGC	6 cut(s)	297, 427, 554, 879, 907, 943
LweI	GCATC	2 cut(s)	410, 892
MaeI	CTAG	5 cut(s)	539, 590, 725, 824, 1019
MaeII	ACGT	1 cut(s)	568
MalI	GATC	4 cut(s)	290, 365, 461, 1047
MboI	GATC	4 cut(s)	288, 363, 459, 1045
MboII	GAAGA	7 cut(s)	12, 174, 465, 859, 862, 1077, 1126
MfeI	CAATTG	2 cut(s)	561, 1098
MhlI	GDGCHC	3 cut(s)	589, 601, 701
MlsI	TGGCCA	2 cut(s)	615, 627
MluCI	AATT	4 cut(s)	561, 879, 1059, 1098
MluNI	TGGCCA	2 cut(s)	615, 627
Mly113I	GGCGCC	1 cut(s)	203
MlyI	GAGTC	3 cut(s)	69, 83, 827
MmeI	TCCRAC	4 cut(s)	240, 434, 574, 820
MnlI	CCTC	5 cut(s)	28, 67, 77, 611, 938
Mox20I	TGGCCA	2 cut(s)	615, 627
Mph1103I	ATGCAT	1 cut(s)	907
MroXI	GAANNNNTTC	1 cut(s)	9
MscI	TGGCCA	2 cut(s)	615, 627
MseI	TTAA	3 cut(s)	368, 747, 1040
Msp20I	TGGCCA	2 cut(s)	615, 627
MspA1I	CMGCKG	2 cut(s)	392, 437
MspI	CCGG	1 cut(s)	243
MspR9I	CCNGG	5 cut(s)	244, 298, 629, 965, 1091
MunI	CAATTG	2 cut(s)	561, 1098
Mva1269I	GAATGC	1 cut(s)	959
MvaI	CCWGG	4 cut(s)	298, 629, 965, 1091
MvnI	CGCG	1 cut(s)	240
MwoI	GCNNNNNNNGC	7 cut(s)	341, 350, 392, 398, 548, 869, 956
NarI	GGCGCC	1 cut(s)	203
NciI	CCSGG	1 cut(s)	244
NdeII	GATC	4 cut(s)	288, 363, 459, 1045
NlaIII	CATG	4 cut(s)	489, 620, 1029, 1080
NlaIV	GGNNCC	7 cut(s)	140, 204, 264, 528, 552, 598, 985
NmeAIII	GCCGAG	1 cut(s)	71
NsbI	TGCGCA	1 cut(s)	314
NsiI	ATGCAT	1 cut(s)	907
PaeR7I	CTCGAG	1 cut(s)	942
PcsI	WCGNNNNNNNCGW	2 cut(s)	79, 206
PctI	GAATGC	1 cut(s)	959
PdmI	GAANNNNTTC	1 cut(s)	9
PfeI	GAWTC	2 cut(s)	277, 302
PkrI	GCNGC	8 cut(s)	312, 394, 439, 442, 544, 894, 897, 958
PleI	GAGTC	3 cut(s)	69, 83, 826
PluTI	GGCGCC	1 cut(s)	206
PpsI	GAGTC	3 cut(s)	69, 83, 826
Psp6I	CCWGG	4 cut(s)	296, 627, 963, 1089
PspFI	CCCAGC	1 cut(s)	433
PspGI	CCWGG	4 cut(s)	296, 627, 963, 1089
PspN4I	GGNNCC	7 cut(s)	140, 204, 264, 528, 552, 598, 985
PspPI	GGNCC	3 cut(s)	139, 527, 984
PstI	CTGCAG	1 cut(s)	897
PstNI	CAGNNNCTG	1 cut(s)	440
RsaI	GTAC	1 cut(s)	571
RsaNI	GTAC	1 cut(s)	570
SalI	GTCGAC	1 cut(s)	72
SaqAI	TTAA	3 cut(s)	368, 747, 1040
SatI	GCNGC	8 cut(s)	311, 393, 438, 441, 543, 893, 896, 957
Sau3AI	GATC	4 cut(s)	288, 363, 459, 1045
Sau96I	GGNCC	3 cut(s)	139, 527, 984
SchI	GAGTC	3 cut(s)	69, 83, 827
ScrFI	CCNGG	5 cut(s)	244, 298, 629, 965, 1091
SduI	GDGCHC	3 cut(s)	589, 601, 701
SfaNI	GCATC	2 cut(s)	410, 892
SfcI	CTRYAG	2 cut(s)	834, 893
SfoI	GGCGCC	1 cut(s)	204
Sfr274I	CTCGAG	1 cut(s)	942
SinI	GGWCC	2 cut(s)	139, 984
SlaI	CTCGAG	1 cut(s)	942
SmlI	CTYRAG	1 cut(s)	942
SmoI	CTYRAG	1 cut(s)	942
SpeI	ACTAGT	1 cut(s)	724
Sse9I	AATT	4 cut(s)	561, 879, 1059, 1098
SsiI	CCGC	6 cut(s)	111, 142, 238, 392, 437, 574
SspDI	GGCGCC	1 cut(s)	202
SspMI	CTAG	5 cut(s)	539, 590, 725, 824, 1019
StyD4I	CCNGG	5 cut(s)	242, 296, 627, 963, 1089
TaaI	ACNGT	3 cut(s)	838, 988, 1012
TaiI	ACGT	1 cut(s)	571
TaqI	TCGA	5 cut(s)	73, 209, 233, 943, 1048
TasI	AATT	4 cut(s)	561, 879, 1059, 1098
TauI	GCSGC	2 cut(s)	395, 440
TfiI	GAWTC	2 cut(s)	277, 302
Tru1I	TTAA	3 cut(s)	368, 747, 1040
Tru9I	TTAA	3 cut(s)	368, 747, 1040
TscAI	CASTG	3 cut(s)	334, 430, 880
TseI	GCWGC	6 cut(s)	310, 440, 542, 892, 895, 956
TspDTI	ATGAA	3 cut(s)	186, 662, 1014
TspRI	CASTG	3 cut(s)	334, 430, 880
VpaK11BI	GGWCC	2 cut(s)	139, 984
XbaI	TCTAGA	1 cut(s)	823
XcmI	CCANNNNNNNNNTGG	1 cut(s)	635
XhoI	CTCGAG	1 cut(s)	942
XmiI	GTMKAC	1 cut(s)	73
XmnI	GAANNNNTTC	1 cut(s)	9
XspI	CTAG	5 cut(s)	539, 590, 725, 824, 1019
Zsp2I	ATGCAT	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.