AT2G27280

Nuclear speckle splicing regulatory protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008231
Physical Location & Seq
Reverse (-)
11673826 .. 11675613
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27280.1

Sequence Viewer

Length: 1284 bp
ATGGAGGAGGCGAGACTCTCGACGTTACCTTTTAGTGCAAGTTTTAATCCTTCAAACCCATTAGGGTTTTTAGAGAATGTCCTTGACTTTATTGGGAAAGAGAGTAACTTTCTGAGGAAAGACACGGCTGAGAAAGAGATCACCGATGCTGTTACTACCGCAAAGGAGAGGTTGAGGGAAACGGAGAAGAAGACGGAGTCCATGGATGTGGAGAAGGTTAGACCCTCGACATTGCCGTTTAATGCAAGTTTTGATCCTTCAGATCCATTAGGGTTCTTGGAGAAAGTCTTTGAATTTGTTGGGAAGAAGAGCAATTTTCTAGTGAAAGACAAGGCTGTGAATGCGATCATCACTGCTGTTACGGACGCCAAGGAGAGGTTGAAGGAAGAGGAGAAGGAGAGTGTAAAGCAAGCAACAGTAAAGATTAAGAAGTACGGACTGCAAATTAGGGCTCCGTCACAGAAAAAGCAATCATCATCTCGACCTCTACTTCGGACTGCTTCAATATTTGGTGAGGATGATGAAGAAAACGATGTGGAGAAGGAGATTTCTCGACAAGCTTCAAAGACCAAGTCTCTTAAAAAGATTGAGAAGCAGCATAAGAAAGCTATAGAGGAGGATCCTTCTGCTTTTGCATACGATGAAGTTTATGATGACATTAAACATGAAGCGGCTCTTCCACGAATGCAAGATCGTGAAGAGCACAAGTCAAGATATATACAGCATATTATGAAACAAGCAGAACGTAGAGAGAAAGAACATGAGATAGTTTATGAGAGAAAGCTTGCGAAAGAAAGAGCGAAAGACGAACATCTTTATTCGGATAAAGAAAAGTTTGTAACTGGTCCCTTTAAAAGAAAACTAGAGGAACAAAAGAAATGGCTGGAGGAAGAAAGATTGCGTGAACTTCGAGAGGAAAGAGATGATGTTACAAAGAAGAACGACTTGAGTGAATTCTACATCAACATTGGAAAAAATGTGGCATTTGGAGCTCGAGATATCGAAGCTAGAGAGGCAGGGAGGCTCAAGGAGCTGAGAAAGGTAGATAGGCTTGAGGAGCTGAGAAAAGAAGAGACAAGGAAAGAGAAGAAAAGAAAATCACCGGAGAAGGAAGTGTCCCCTGACTCAGGAGATTTTGGATTAAGTAGTAAAAAAAGCGTCAAACCGCAAGACGCATCAATCAAAGAGGAAGCAAAAGAAACGCAGAAAGCCACTAGAGAGGATGCGATAGCGACTGCAAAAGAACGGTTTCTATCCCGTAAGAAGGCAAAGATAGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2040

Protein Analysis

427

Amino Acids

49.63

Weight (kDa)

8.96

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSRP1_N PF09745 197 - 313 4.1e-34 Nuclear speckle splicing regulatory protein 1, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 159, 671, 1166
AclWI GGATC 4 cut(s) 248, 257, 614, 627
AcsI RAATTY 2 cut(s) 293, 953
AcuI CTGAAG 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 375, 1127, 1264
AgsI TTSAA 5 cut(s) 54, 293, 382, 504, 564
AluBI AGCT 7 cut(s) 560, 608, 784, 992, 1007, 1033, 1060
AluI AGCT 7 cut(s) 560, 608, 784, 992, 1007, 1033, 1060
Alw21I GWGCWC 2 cut(s) 705, 994
Alw26I GTCTC 3 cut(s) 7, 579, 1067
AlwI GGATC 4 cut(s) 248, 257, 614, 627
Ama87I CYCGRG 1 cut(s) 993
ApeKI GCWGC 1 cut(s) 595
ApoI RAATTY 2 cut(s) 293, 953
ArsI GACNNNNNNTTYG 4 cut(s) 474, 506, 557, 589
Asp700I GAANNNNTTC 1 cut(s) 1248
AspS9I GGNCC 1 cut(s) 845
AsuHPI GGTGA 3 cut(s) 133, 524, 1092
AvaI CYCGRG 1 cut(s) 993
AvaII GGWCC 1 cut(s) 845
BamHI GGATCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 454, 994
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 2 cut(s) 705, 994
BbvI GCAGC 1 cut(s) 607
BceAI ACGGC 2 cut(s) 141, 220
BcoDI GTCTC 3 cut(s) 7, 579, 1067
BfaI CTAG 4 cut(s) 320, 863, 1008, 1215
BfmI CTRYAG 1 cut(s) 609
BisI GCNGC 2 cut(s) 596, 672
BlsI GCNGC 2 cut(s) 597, 673
Bme18I GGWCC 1 cut(s) 845
BmeT110I CYCGRG 1 cut(s) 993
BmgT120I GGNCC 1 cut(s) 845
BmiI GGNNCC 3 cut(s) 453, 621, 847
BmsI GCATC 3 cut(s) 136, 1184, 1213
BpiI GAAGAC 1 cut(s) 197
BpmI CTGGAG 1 cut(s) 905
BpuEI CTTGAG 3 cut(s) 967, 1010, 1073
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 201, 369
BsaWI WCCGGW 1 cut(s) 1102
Bsc4I CCNNNNNNNGG 3 cut(s) 375, 1127, 1264
Bse1I ACTGG 1 cut(s) 847
Bse3DI GCAATG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 201, 369
BseGI GGATG 3 cut(s) 211, 523, 1228
BseLI CCNNNNNNNGG 3 cut(s) 375, 1127, 1264
BseMI GCAATG 1 cut(s) 230
BseMII CTCAG 5 cut(s) 104, 120, 1025, 1052, 1140
BseNI ACTGG 1 cut(s) 847
BseRI GAGGAG 4 cut(s) 20, 404, 629, 1070
BseXI GCAGC 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 705, 994
BsiHKCI CYCGRG 1 cut(s) 993
BsiSI CCGG 1 cut(s) 1103
BslFI GGGAC 2 cut(s) 831, 1102
BslI CCNNNNNNNGG 3 cut(s) 375, 1127, 1264
BsmAI GTCTC 3 cut(s) 7, 579, 1067
BsmFI GGGAC 2 cut(s) 831, 1102
BsmI GAATGC 2 cut(s) 346, 690
BsoBI CYCGRG 1 cut(s) 993
Bsp1286I GDGCHC 3 cut(s) 454, 705, 994
Bsp143I GATC 6 cut(s) 138, 253, 262, 345, 619, 691
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 3 cut(s) 159, 671, 1166
BspCNI CTCAG 5 cut(s) 105, 121, 1026, 1053, 1139
BspLI GGNNCC 3 cut(s) 453, 621, 847
BspPI GGATC 4 cut(s) 248, 257, 614, 627
BspQI GCTCTTC 3 cut(s) 302, 681, 693
BsrDI GCAATG 1 cut(s) 230
BsrI ACTGG 1 cut(s) 847
BssECI CCNNGG 2 cut(s) 201, 369
BssMI GATC 6 cut(s) 138, 253, 262, 345, 619, 691
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 2 cut(s) 201, 369
Bst4CI ACNGT 2 cut(s) 418, 1248
Bst6I CTCTTC 5 cut(s) 302, 381, 681, 693, 1065
BstACI GRCGYC 1 cut(s) 366
BstC8I GCNNGC 2 cut(s) 411, 786
BstDEI CTNAG 5 cut(s) 113, 129, 1034, 1061, 1126
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 1125
BstF5I GGATG 3 cut(s) 211, 523, 1228
BstKTI GATC 6 cut(s) 141, 256, 265, 348, 622, 694
BstMAI GTCTC 3 cut(s) 7, 579, 1067
BstMBI GATC 6 cut(s) 138, 253, 262, 345, 619, 691
BstMWI GCNNNNNNNGC 5 cut(s) 341, 989, 1013, 1030, 1057
BstSFI CTRYAG 1 cut(s) 609
BstV1I GCAGC 1 cut(s) 607
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 2 cut(s) 262, 619
BstXI CCANNNNNNTGG 1 cut(s) 208
BstYI RGATCY 2 cut(s) 262, 619
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 3 cut(s) 211, 523, 1228
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 411, 786
Cfr13I GGNCC 1 cut(s) 845
CseI GACGC 3 cut(s) 374, 1147, 1181
Csp6I GTAC 1 cut(s) 433
CviAII CATG 3 cut(s) 202, 665, 761
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 5 cut(s) 113, 129, 1034, 1061, 1126
DpnI GATC 6 cut(s) 140, 255, 264, 347, 621, 693
DpnII GATC 6 cut(s) 138, 253, 262, 345, 619, 691
DraI TTTAAA 1 cut(s) 853
Eam1104I CTCTTC 5 cut(s) 302, 381, 681, 693, 1065
EarI CTCTTC 5 cut(s) 302, 381, 681, 693, 1065
Ecl136II GAGCTC 1 cut(s) 992
Eco130I CCWWGG 2 cut(s) 201, 369
Eco24I GRGCYC 2 cut(s) 454, 994
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 1 cut(s) 845
Eco53kI GAGCTC 1 cut(s) 992
Eco57I CTGAAG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 993
EcoICRI GAGCTC 1 cut(s) 992
EcoNI CCTNNNNNAGG 1 cut(s) 1125
EcoRI GAATTC 1 cut(s) 953
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 2 cut(s) 201, 369
EcoT38I GRGCYC 2 cut(s) 454, 994
ErhI CCWWGG 2 cut(s) 201, 369
FaeI CATG 3 cut(s) 205, 668, 764
FalI AAGNNNNNCTT 4 cut(s) 660, 692, 929, 961
FaqI GGGAC 2 cut(s) 831, 1102
FatI CATG 3 cut(s) 201, 664, 760
Fnu4HI GCNGC 2 cut(s) 596, 672
FokI GGATG 3 cut(s) 218, 530, 1235
FriOI GRGCYC 2 cut(s) 454, 994
Fsp4HI GCNGC 2 cut(s) 596, 672
FspBI CTAG 4 cut(s) 320, 863, 1008, 1215
GluI GCNGC 2 cut(s) 596, 672
GsuI CTGGAG 1 cut(s) 905
HapII CCGG 1 cut(s) 1103
HgaI GACGC 3 cut(s) 374, 1147, 1181
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 3 cut(s) 205, 668, 764
HindIII AAGCTT 2 cut(s) 558, 782
HinfI GANTC 3 cut(s) 15, 197, 1124
HpaII CCGG 1 cut(s) 1103
HphI GGTGA 3 cut(s) 133, 524, 1092
Hpy166II GTNNAC 1 cut(s) 905
Hpy188I TCNGA 4 cut(s) 114, 262, 495, 823
Hpy188III TCNNGA 8 cut(s) 19, 480, 552, 695, 711, 911, 995, 1128
Hpy8I GTNNAC 1 cut(s) 905
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 9 cut(s) 60, 208, 267, 376, 388, 535, 633, 1102, 1258
HpyCH4III ACNGT 2 cut(s) 418, 1248
HpyCH4IV ACGT 2 cut(s) 23, 745
HpyCH4V TGCA 6 cut(s) 38, 245, 442, 635, 688, 1238
HpyF10VI GCNNNNNNNGC 5 cut(s) 341, 989, 1013, 1030, 1057
HpyF3I CTNAG 5 cut(s) 113, 129, 1034, 1061, 1126
HpySE526I ACGT 2 cut(s) 23, 745
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 3 cut(s) 205, 668, 764
Kzo9I GATC 6 cut(s) 138, 253, 262, 345, 619, 691
LguI GCTCTTC 3 cut(s) 302, 681, 693
LmnI GCTCC 4 cut(s) 457, 989, 1030, 1057
LpnPI CCDG 6 cut(s) 828, 869, 1002, 1113, 1116, 1134
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 3 cut(s) 136, 1184, 1213
MaeI CTAG 4 cut(s) 320, 863, 1008, 1215
MaeII ACGT 2 cut(s) 23, 745
MaeIII GTNAC 7 cut(s) 24, 104, 151, 358, 456, 838, 928
MalI GATC 6 cut(s) 140, 255, 264, 347, 621, 693
MboI GATC 6 cut(s) 138, 253, 262, 345, 619, 691
MflI RGATCY 2 cut(s) 262, 619
MhlI GDGCHC 3 cut(s) 454, 705, 994
MluCI AATT 4 cut(s) 293, 313, 444, 953
MlyI GAGTC 3 cut(s) 9, 206, 1118
MroXI GAANNNNTTC 1 cut(s) 1248
MseI TTAA 7 cut(s) 45, 240, 426, 579, 660, 852, 1142
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 1 cut(s) 1103
Mva1269I GAATGC 2 cut(s) 346, 690
MwoI GCNNNNNNNGC 5 cut(s) 341, 989, 1013, 1030, 1057
NcoI CCATGG 1 cut(s) 201
NdeII GATC 6 cut(s) 138, 253, 262, 345, 619, 691
NlaIII CATG 3 cut(s) 205, 668, 764
NlaIV GGNNCC 3 cut(s) 453, 621, 847
NmuCI GTSAC 1 cut(s) 456
PaeR7I CTCGAG 1 cut(s) 993
PciSI GCTCTTC 3 cut(s) 302, 681, 693
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PctI GAATGC 2 cut(s) 346, 690
PdmI GAANNNNTTC 1 cut(s) 1248
PflFI GACNNNGTC 2 cut(s) 196, 571
PkrI GCNGC 2 cut(s) 597, 673
PleI GAGTC 3 cut(s) 9, 205, 1118
PpsI GAGTC 3 cut(s) 9, 205, 1118
Psp124BI GAGCTC 1 cut(s) 994
PspN4I GGNNCC 3 cut(s) 453, 621, 847
PspPI GGNCC 1 cut(s) 845
PsuI RGATCY 2 cut(s) 262, 619
PsyI GACNNNGTC 2 cut(s) 196, 571
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 994
SapI GCTCTTC 3 cut(s) 302, 681, 693
SaqAI TTAA 7 cut(s) 45, 240, 426, 579, 660, 852, 1142
SatI GCNGC 2 cut(s) 596, 672
Sau3AI GATC 6 cut(s) 138, 253, 262, 345, 619, 691
Sau96I GGNCC 1 cut(s) 845
SchI GAGTC 3 cut(s) 9, 206, 1118
SduI GDGCHC 3 cut(s) 454, 705, 994
SfaNI GCATC 3 cut(s) 136, 1184, 1213
SfcI CTRYAG 1 cut(s) 609
Sfr274I CTCGAG 1 cut(s) 993
SinI GGWCC 1 cut(s) 845
SlaI CTCGAG 1 cut(s) 993
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 4 cut(s) 946, 993, 1025, 1052
SmoI CTYRAG 4 cut(s) 946, 993, 1025, 1052
Sse9I AATT 4 cut(s) 293, 313, 444, 953
SsiI CCGC 3 cut(s) 159, 671, 1166
SspI AATATT 1 cut(s) 507
SspMI CTAG 4 cut(s) 320, 863, 1008, 1215
SstI GAGCTC 1 cut(s) 994
StyI CCWWGG 2 cut(s) 201, 369
TaaI ACNGT 2 cut(s) 418, 1248
TaiI ACGT 2 cut(s) 26, 748
TaqI TCGA 7 cut(s) 20, 227, 481, 553, 910, 994, 1002
TasI AATT 4 cut(s) 293, 313, 444, 953
TauI GCSGC 1 cut(s) 674
Tru1I TTAA 7 cut(s) 45, 240, 426, 579, 660, 852, 1142
Tru9I TTAA 7 cut(s) 45, 240, 426, 579, 660, 852, 1142
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 456
TseI GCWGC 1 cut(s) 595
Tsp45I GTSAC 1 cut(s) 456
TspDTI ATGAA 4 cut(s) 537, 657, 681, 746
TspGWI ACGGA 5 cut(s) 197, 209, 377, 444, 450
TspRI CASTG 1 cut(s) 358
Tth111I GACNNNGTC 2 cut(s) 196, 571
VpaK11BI GGWCC 1 cut(s) 845
XagI CCTNNNNNAGG 1 cut(s) 1125
XapI RAATTY 2 cut(s) 293, 953
XhoI CTCGAG 1 cut(s) 993
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 4 cut(s) 320, 863, 1008, 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.