MD15G1329700.v1.1

Nuclear speckle splicing regulatory protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008231
Physical Location & Seq
Reverse (-)
36304764 .. 36307097
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1329700.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGATTAAGTACGGGTTGAACCTAAGGGGGCAGCAGAAGAAGCAGCCGACAAGGCCTCCTGTCCCTAAACCTCTGGGTTTTGGTGACGATGACGACGACAATGATGTTGAGAAGGAAATATCTCGCCAGGCTTCCAAGAACAAATCTCTCAAGGATATTGAGGAACAGCAGAGGAAGGCACTGGAGCAGGACCCGTCGGTGTTCGACTATGATGGAGTTTATGACCAGATGAAACAGAAAGCCATCCAACCGCGGGTGCAAGATCGTCAAGAGAGAATGGCAAGGTATATTCCTGGTATACAGAGAAAGGCAGAAGAACGAAAGCGAGAGCAGGAGATAATATACGAGAGAAAACTACAGAAAGAGAGAAACCAAGAGGATCACCTCTATGCAGACAAGGAACGCTTTGTCACAGGTGCTTACAAAAAGAAACTTGCAGAGCAAGCAAAATGGGCAGAGGAAGATCGACTGCGTCAACTTCGGGAGGAGAAAGATGATGTTACCAAGAAGAAAGACTTGACTGATTTCTACTTCAACCTTCAAAAAAATGTTGCTTTTGGTGCAGAAAATGTGAAGTCCAGGAAGCTAGAGAAGCAGGAGGAATTAAGCAGGCCAGATAAACATGAAGAAGATAGAGATAGACCAATTGCCGTAGCATTGGAGCCATTGGGGCCTTCCAGTGTGAGAGAGGGGCATCTAGAGAAGCAGACTGAATCAGGCAAGCCAGACAAACTTGAAGACAGAGTAATCACTGCAGCATCAGTGCCGTTGGAGTCTCCCAGGGTGATAGAAGGACATCTAGATGAAAGTTTAACCAAGTCTAAACGTCGTGATGAGCTCTCTGAAGAAAAGCAAGCTTCAAATCCTTCAGCACAGGAAAAACCGTCAGTTGAACAGCCGCCAGAAAACCAACCGAGTACTAATCATCATAAAAGAAGTGCAGATGACGTGGCTGCAGCGAGAGAACGCTATCTAGCAAGGAAGAGACCTAAAGAACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2040

Protein Analysis

334

Amino Acids

38.83

Weight (kDa)

7.76

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSRP1_N PF09745 54 - 170 5.2e-36 Nuclear speckle splicing regulatory protein 1, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 298
AccII CGCG 1 cut(s) 253
AciI CCGC 3 cut(s) 251, 253, 899
AclWI GGATC 1 cut(s) 387
AcuI CTGAAG 2 cut(s) 852, 864
AfaI GTAC 2 cut(s) 11, 919
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 6 cut(s) 19, 535, 542, 737, 861, 893
AjiI CACGTC 1 cut(s) 949
AjnI CCWGG 4 cut(s) 126, 292, 578, 779
AluBI AGCT 3 cut(s) 586, 838, 857
AluI AGCT 3 cut(s) 586, 838, 857
Alw21I GWGCWC 1 cut(s) 840
Alw26I GTCTC 2 cut(s) 780, 979
AlwI GGATC 1 cut(s) 387
AoxI GGCC 3 cut(s) 53, 611, 671
ApeKI GCWGC 5 cut(s) 31, 43, 755, 953, 956
AspS9I GGNCC 2 cut(s) 190, 671
AsuHPI GGTGA 3 cut(s) 95, 374, 796
AvaII GGWCC 1 cut(s) 190
AxyI CCTNAGG 1 cut(s) 23
BanII GRGCYC 1 cut(s) 840
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 840
BbvI GCAGC 5 cut(s) 43, 55, 767, 940, 968
BccI CCATC 2 cut(s) 206, 251
BceAI ACGGC 2 cut(s) 635, 751
BciT130I CCWGG 4 cut(s) 128, 294, 580, 781
BcoDI GTCTC 2 cut(s) 780, 979
BfaI CTAG 4 cut(s) 587, 698, 800, 974
BfmI CTRYAG 3 cut(s) 356, 753, 954
BglI GCCNNNNNGGC 2 cut(s) 52, 670
BisI GCNGC 6 cut(s) 32, 44, 756, 899, 954, 957
BlsI GCNGC 6 cut(s) 33, 45, 757, 900, 955, 958
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 4 cut(s) 128, 294, 580, 781
Bme18I GGWCC 1 cut(s) 190
BmgBI CACGTC 1 cut(s) 949
BmgT120I GGNCC 2 cut(s) 190, 671
BmiI GGNNCC 3 cut(s) 192, 663, 672
BmrFI CCNGG 4 cut(s) 128, 294, 580, 781
BmsI GCATC 2 cut(s) 703, 767
BpiI GAAGAC 1 cut(s) 744
BpmI CTGGAG 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 979
BsaJI CCNNGG 3 cut(s) 251, 779, 780
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 1 cut(s) 253
Bse1I ACTGG 2 cut(s) 186, 678
Bse21I CCTNAGG 1 cut(s) 23
BseBI CCWGG 4 cut(s) 128, 294, 580, 781
BseDI CCNNGG 3 cut(s) 251, 779, 780
BseGI GGATG 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 253
BseNI ACTGG 2 cut(s) 186, 678
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 5 cut(s) 43, 55, 767, 940, 968
BsgI GTGCAG 2 cut(s) 582, 960
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 3 cut(s) 55, 613, 673
BsiHKAI GWGCWC 1 cut(s) 840
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 1 cut(s) 253
BsmAI GTCTC 2 cut(s) 780, 979
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 3 cut(s) 55, 613, 673
Bso31I GGTCTC 1 cut(s) 979
Bsp1286I GDGCHC 1 cut(s) 840
Bsp143I GATC 3 cut(s) 262, 379, 463
BspACI CCGC 3 cut(s) 251, 253, 899
BspANI GGCC 3 cut(s) 55, 613, 673
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 192, 663, 672
BspMAI CTGCAG 2 cut(s) 757, 958
BspPI GGATC 1 cut(s) 387
BspTNI GGTCTC 1 cut(s) 979
BsrI ACTGG 2 cut(s) 186, 678
BssECI CCNNGG 3 cut(s) 251, 779, 780
BssMI GATC 3 cut(s) 262, 379, 463
BssNAI GTATAC 1 cut(s) 299
Bst1107I GTATAC 1 cut(s) 299
Bst2UI CCWGG 4 cut(s) 128, 294, 580, 781
Bst4CI ACNGT 1 cut(s) 885
Bst6I CTCTTC 1 cut(s) 977
BstC8I GCNNGC 4 cut(s) 444, 611, 722, 855
BstDEI CTNAG 1 cut(s) 23
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 1 cut(s) 243
BstFNI CGCG 1 cut(s) 253
BstKTI GATC 3 cut(s) 265, 382, 466
BstMAI GTCTC 2 cut(s) 780, 979
BstMBI GATC 3 cut(s) 262, 379, 463
BstMWI GCNNNNNNNGC 7 cut(s) 40, 52, 443, 452, 560, 592, 670
BstNI CCWGG 4 cut(s) 128, 294, 580, 781
BstSCI CCNGG 4 cut(s) 126, 292, 578, 779
BstSFI CTRYAG 3 cut(s) 356, 753, 954
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 5 cut(s) 43, 55, 767, 940, 968
BstV2I GAAGAC 1 cut(s) 744
BstZ17I GTATAC 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 23
BsuRI GGCC 3 cut(s) 55, 613, 673
BtgI CCRYGG 1 cut(s) 251
BtrI CACGTC 1 cut(s) 949
BtsCI GGATG 1 cut(s) 243
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 4 cut(s) 179, 685, 750, 768
Cac8I GCNNGC 4 cut(s) 444, 611, 722, 855
Cfr13I GGNCC 2 cut(s) 190, 671
Cfr42I CCGCGG 1 cut(s) 254
CseI GACGC 1 cut(s) 461
Csp6I GTAC 2 cut(s) 10, 918
CviAII CATG 1 cut(s) 623
CviQI GTAC 2 cut(s) 10, 918
DdeI CTNAG 1 cut(s) 23
DpnI GATC 3 cut(s) 264, 381, 465
DpnII GATC 3 cut(s) 262, 379, 463
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
Ecl136II GAGCTC 1 cut(s) 838
Eco147I AGGCCT 1 cut(s) 55
Eco24I GRGCYC 1 cut(s) 840
Eco31I GGTCTC 1 cut(s) 979
Eco47I GGWCC 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 852, 864
Eco81I CCTNAGG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 838
EcoO109I RGGNCCY 2 cut(s) 190, 671
EcoRII CCWGG 4 cut(s) 126, 292, 578, 779
EcoT38I GRGCYC 1 cut(s) 840
FaeI CATG 1 cut(s) 626
FaiI YATR 9 cut(s) 210, 222, 288, 299, 343, 390, 624, 930, 1000
FalI AAGNNNNNCTT 4 cut(s) 389, 421, 500, 532
FaqI GGGAC 1 cut(s) 47
FatI CATG 1 cut(s) 622
FauI CCCGC 1 cut(s) 246
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 6 cut(s) 32, 44, 756, 899, 954, 957
FokI GGATG 1 cut(s) 230
FriOI GRGCYC 1 cut(s) 840
Fsp4HI GCNGC 6 cut(s) 32, 44, 756, 899, 954, 957
FspBI CTAG 4 cut(s) 587, 698, 800, 974
GluI GCNGC 6 cut(s) 32, 44, 756, 899, 954, 957
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 3 cut(s) 55, 613, 673
HgaI GACGC 1 cut(s) 461
Hin1II CATG 1 cut(s) 626
HincII GTYRAC 1 cut(s) 476
HindII GTYRAC 1 cut(s) 476
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 2 cut(s) 713, 773
HphI GGTGA 3 cut(s) 95, 374, 796
Hpy166II GTNNAC 2 cut(s) 299, 476
Hpy188I TCNGA 1 cut(s) 844
Hpy188III TCNNGA 5 cut(s) 269, 482, 698, 800, 830
Hpy8I GTNNAC 2 cut(s) 299, 476
Hpy99I CGWCG 3 cut(s) 98, 199, 831
HpyAV CCTTC 6 cut(s) 106, 169, 548, 684, 785, 876
HpyCH4III ACNGT 1 cut(s) 885
HpyCH4IV ACGT 2 cut(s) 826, 948
HpyCH4V TGCA 7 cut(s) 259, 392, 437, 563, 755, 941, 956
HpyF10VI GCNNNNNNNGC 7 cut(s) 40, 52, 443, 452, 560, 592, 670
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 826, 948
Hsp92II CATG 1 cut(s) 626
KspI CCGCGG 1 cut(s) 254
Kzo9I GATC 3 cut(s) 262, 379, 463
LmnI GCTCC 2 cut(s) 184, 661
Lsp1109I GCAGC 5 cut(s) 43, 55, 767, 940, 968
LweI GCATC 2 cut(s) 703, 767
MaeI CTAG 4 cut(s) 587, 698, 800, 974
MaeII ACGT 2 cut(s) 826, 948
MaeIII GTNAC 3 cut(s) 83, 409, 499
MalI GATC 3 cut(s) 264, 381, 465
MboI GATC 3 cut(s) 262, 379, 463
MboII GAAGA 9 cut(s) 49, 326, 473, 520, 638, 641, 749, 857, 994
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 840
MluCI AATT 2 cut(s) 602, 645
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 2 cut(s) 271, 750
MseI TTAA 3 cut(s) 6, 605, 812
MslI CAYNNNNRTG 2 cut(s) 387, 801
MspA1I CMGCKG 1 cut(s) 253
MspR9I CCNGG 4 cut(s) 128, 294, 580, 781
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 4 cut(s) 128, 294, 580, 781
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 7 cut(s) 40, 52, 443, 452, 560, 592, 670
NdeII GATC 3 cut(s) 262, 379, 463
NlaIII CATG 1 cut(s) 626
NlaIV GGNNCC 3 cut(s) 192, 663, 672
NmuCI GTSAC 2 cut(s) 83, 409
PasI CCCWGGG 1 cut(s) 780
PceI AGGCCT 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 1 cut(s) 713
PflFI GACNNNGTC 1 cut(s) 471
PfoI TCCNGGA 1 cut(s) 578
PkrI GCNGC 6 cut(s) 33, 45, 757, 900, 955, 958
PleI GAGTC 1 cut(s) 781
PpsI GAGTC 1 cut(s) 781
PpuMI RGGWCCY 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 4 cut(s) 126, 292, 578, 779
PspGI CCWGG 4 cut(s) 126, 292, 578, 779
PspN4I GGNNCC 3 cut(s) 192, 663, 672
PspPI GGNCC 2 cut(s) 190, 671
PspPPI RGGWCCY 1 cut(s) 190
PstI CTGCAG 2 cut(s) 757, 958
PsyI GACNNNGTC 1 cut(s) 471
RsaI GTAC 2 cut(s) 11, 919
RsaNI GTAC 2 cut(s) 10, 918
RseI CAYNNNNRTG 2 cut(s) 387, 801
SacI GAGCTC 1 cut(s) 840
SacII CCGCGG 1 cut(s) 254
SaqAI TTAA 3 cut(s) 6, 605, 812
SatI GCNGC 6 cut(s) 32, 44, 756, 899, 954, 957
Sau3AI GATC 3 cut(s) 262, 379, 463
Sau96I GGNCC 2 cut(s) 190, 671
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 4 cut(s) 128, 294, 580, 781
SduI GDGCHC 1 cut(s) 840
SfaNI GCATC 2 cut(s) 703, 767
SfcI CTRYAG 3 cut(s) 356, 753, 954
Sfr303I CCGCGG 1 cut(s) 254
SgrBI CCGCGG 1 cut(s) 254
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 387, 801
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 2 cut(s) 602, 645
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 3 cut(s) 251, 253, 899
SspMI CTAG 4 cut(s) 587, 698, 800, 974
SstI GAGCTC 1 cut(s) 840
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 4 cut(s) 126, 292, 578, 779
TaaI ACNGT 1 cut(s) 885
TaiI ACGT 2 cut(s) 829, 951
TaqI TCGA 2 cut(s) 204, 466
TasI AATT 2 cut(s) 602, 645
TatI WGTACW 1 cut(s) 917
TauI GCSGC 1 cut(s) 901
TfiI GAWTC 1 cut(s) 713
Tru1I TTAA 3 cut(s) 6, 605, 812
Tru9I TTAA 3 cut(s) 6, 605, 812
TscAI CASTG 4 cut(s) 186, 685, 757, 768
TseFI GTSAC 2 cut(s) 83, 409
TseI GCWGC 5 cut(s) 31, 43, 755, 953, 956
Tsp45I GTSAC 2 cut(s) 83, 409
TspDTI ATGAA 3 cut(s) 245, 639, 819
TspRI CASTG 4 cut(s) 186, 685, 757, 768
Tth111I GACNNNGTC 1 cut(s) 471
VpaK11BI GGWCC 1 cut(s) 190
XbaI TCTAGA 2 cut(s) 697, 799
XmiI GTMKAC 1 cut(s) 298
XspI CTAG 4 cut(s) 587, 698, 800, 974
ZrmI AGTACT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.