pycom01g05120

Nuclear speckle splicing regulatory protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008231
Physical Location & Seq
Forward (+)
4693785 .. 4694822
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05120.1

Sequence Viewer

Length: 975 bp
ATGAATTTGGAAGCAGAGCATCACAGGAAAAAGAAGAAGACGAGGTTGAACCTAAGGGGGCAGCAGAAGAAGCAGCCGACGAGGCCTCCTCTCCCTAAACCTCGGGGTTTTGGCGATGACGATGATGACAATGATGTTGAGAAGGCCCTCGAGCAGGACCCGTCGGTGTTCAACTACGATGGAGTTTATGATGAGATGAAACAGAAAGCCATCCAACCTCGGGTACAAGATCGCCAAGAGAGAATGGCAAGGTACATTCCTGGTATAAGAGAAAAGCAGAAGAACGAAAGCGAAAAGCAGGAGATAATATATGAGAGAAAACTACAGAAAGAGCGAAACCAGGAGGATCACCTCTATGCAGACAAGGAACGCTTTGTCACAGGTGCTTACAAAAGGAAACTTGCAGAGCAAGCCAAATGGGCGGAGGAAGATCGACTGCGTCAACTTCAGGAGGAGAAAGAGAAAGACTTGACTGATTTTTACTTCAACCTTCAAAGAAATGTTGCTTTTGTTGCAGAAGATGTGAAGTCCAGGAAGCTAGAGACGCAGGAGGAATTAAGCAGGCCAGATAAACATGACGATAGAGATGGACCAAATGCTGTAGCATTGGAGCCATTGGGGTCTTCCAGTGTGAGAGAGGTGCATCTAGAAAAGCAGACCGAATCAGGCAAGCCAAACAAACTTGAAGACAGAGAAATAGCTGCAGCATCAGTGCCGTTGGAGTCACTCGGGGTGATAGAGGGACATCAAGAGGAAACTTTAACCAAGTCTAAACGTAGTGATGAGCTCTCTGATGAAAAGAAAGCTTCAATTGCATCAGCACAGGACAAATCGTCAGTTGAACAGCCGCCAGAAAACCGAGTACTAACCACCATAAAGGAAGTGCAGATGATCTGGCTGCAGCGAGAGAACGCTATCTGGCAAGGAAGAGACCAAAAGAATCATAGAATTTCTGCTCGTCATGAACGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2040

Protein Analysis

325

Amino Acids

37.95

Weight (kDa)

6.32

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSRP1_N PF09745 44 - 155 1.3e-30 Nuclear speckle splicing regulatory protein 1, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 422, 848
AclI AACGTT 1 cut(s) 967
AclWI GGATC 1 cut(s) 354
AcsI RAATTY 2 cut(s) 4, 948
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 3 cut(s) 225, 254, 864
AfiI CCNNNNNNNGG 2 cut(s) 154, 220
AgsI TTSAA 7 cut(s) 49, 172, 487, 494, 686, 810, 842
AhdI GACNNNNNGTC 1 cut(s) 832
AjnI CCWGG 3 cut(s) 259, 339, 530
AluBI AGCT 4 cut(s) 538, 701, 787, 806
AluI AGCT 4 cut(s) 538, 701, 787, 806
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 2 cut(s) 536, 924
AlwI GGATC 1 cut(s) 354
Ama87I CYCGRG 4 cut(s) 102, 149, 219, 728
AoxI GGCC 3 cut(s) 83, 144, 563
ApeKI GCWGC 6 cut(s) 61, 73, 701, 704, 898, 901
ApoI RAATTY 2 cut(s) 4, 948
AspS9I GGNCC 3 cut(s) 145, 157, 590
AsuHPI GGTGA 2 cut(s) 341, 745
AvaI CYCGRG 4 cut(s) 102, 149, 219, 728
AvaII GGWCC 2 cut(s) 157, 590
AxyI CCTNAGG 1 cut(s) 53
BanII GRGCYC 1 cut(s) 789
BbsI GAAGAC 3 cut(s) 44, 615, 693
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 6 cut(s) 73, 85, 688, 716, 885, 913
BccI CCATC 3 cut(s) 173, 218, 581
BceAI ACGGC 1 cut(s) 700
BciT130I CCWGG 3 cut(s) 261, 341, 532
BcoDI GTCTC 2 cut(s) 536, 924
BfaI CTAG 2 cut(s) 539, 647
BfmI CTRYAG 4 cut(s) 323, 600, 702, 899
BglI GCCNNNNNGGC 2 cut(s) 82, 419
BisI GCNGC 7 cut(s) 62, 74, 702, 705, 848, 899, 902
BlsI GCNGC 7 cut(s) 63, 75, 703, 706, 849, 900, 903
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 3 cut(s) 261, 341, 532
Bme18I GGWCC 2 cut(s) 157, 590
BmeRI GACNNNNNGTC 1 cut(s) 832
BmeT110I CYCGRG 4 cut(s) 102, 149, 219, 728
BmgT120I GGNCC 3 cut(s) 145, 157, 590
BmiI GGNNCC 2 cut(s) 159, 612
BmrFI CCNGG 3 cut(s) 261, 341, 532
BmsI GCATC 4 cut(s) 28, 652, 716, 824
BpiI GAAGAC 3 cut(s) 44, 615, 693
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 2 cut(s) 101, 218
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 220
Bse1I ACTGG 1 cut(s) 627
Bse21I CCTNAGG 1 cut(s) 53
BseBI CCWGG 3 cut(s) 261, 341, 532
BseDI CCNNGG 2 cut(s) 101, 218
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 154, 220
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 2 cut(s) 78, 467
BseXI GCAGC 6 cut(s) 73, 85, 688, 716, 885, 913
BsgI GTGCAG 1 cut(s) 905
BshFI GGCC 3 cut(s) 85, 146, 565
BsiHKAI GWGCWC 1 cut(s) 789
BsiHKCI CYCGRG 4 cut(s) 102, 149, 219, 728
BslFI GGGAC 1 cut(s) 756
BslI CCNNNNNNNGG 2 cut(s) 154, 220
BsmAI GTCTC 2 cut(s) 536, 924
BsmBI CGTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 756
BsnI GGCC 3 cut(s) 85, 146, 565
Bso31I GGTCTC 1 cut(s) 924
BsoBI CYCGRG 4 cut(s) 102, 149, 219, 728
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 4 cut(s) 229, 346, 430, 891
BspACI CCGC 2 cut(s) 422, 848
BspANI GGCC 3 cut(s) 85, 146, 565
BspHI TCATGA 1 cut(s) 961
BspLI GGNNCC 2 cut(s) 159, 612
BspMAI CTGCAG 2 cut(s) 706, 903
BspPI GGATC 1 cut(s) 354
BspTNI GGTCTC 1 cut(s) 924
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 2 cut(s) 101, 218
BssMI GATC 4 cut(s) 229, 346, 430, 891
Bst2UI CCWGG 3 cut(s) 261, 341, 532
Bst6I CTCTTC 1 cut(s) 922
BstC8I GCNNGC 3 cut(s) 411, 563, 671
BstDEI CTNAG 1 cut(s) 53
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 1 cut(s) 210
BstKTI GATC 4 cut(s) 232, 349, 433, 894
BstMAI GTCTC 2 cut(s) 536, 924
BstMBI GATC 4 cut(s) 229, 346, 430, 891
BstMWI GCNNNNNNNGC 7 cut(s) 70, 82, 410, 419, 512, 544, 812
BstNI CCWGG 3 cut(s) 261, 341, 532
BstSCI CCNGG 3 cut(s) 259, 339, 530
BstSFI CTRYAG 4 cut(s) 323, 600, 702, 899
BstV1I GCAGC 6 cut(s) 73, 85, 688, 716, 885, 913
BstV2I GAAGAC 3 cut(s) 44, 615, 693
Bsu36I CCTNAGG 1 cut(s) 53
BsuRI GGCC 3 cut(s) 85, 146, 565
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 634, 717
Cac8I GCNNGC 3 cut(s) 411, 563, 671
CciI TCATGA 1 cut(s) 961
Cfr13I GGNCC 3 cut(s) 145, 157, 590
CseI GACGC 2 cut(s) 428, 553
Csp6I GTAC 3 cut(s) 224, 253, 863
CviAII CATG 2 cut(s) 575, 962
CviQI GTAC 3 cut(s) 224, 253, 863
DdeI CTNAG 1 cut(s) 53
DpnI GATC 4 cut(s) 231, 348, 432, 893
DpnII GATC 4 cut(s) 229, 346, 430, 891
DriI GACNNNNNGTC 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 922
Eam1105I GACNNNNNGTC 1 cut(s) 832
EarI CTCTTC 1 cut(s) 922
EciI GGCGGA 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 787
Eco147I AGGCCT 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 789
Eco31I GGTCTC 1 cut(s) 924
Eco47I GGWCC 2 cut(s) 157, 590
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 53
Eco88I CYCGRG 4 cut(s) 102, 149, 219, 728
EcoICRI GAGCTC 1 cut(s) 787
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoO109I RGGNCCY 2 cut(s) 145, 157
EcoRII CCWGG 3 cut(s) 259, 339, 530
EcoT38I GRGCYC 1 cut(s) 789
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 2 cut(s) 578, 965
FaiI YATR 9 cut(s) 189, 266, 310, 312, 357, 576, 875, 945, 963
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 756
FatI CATG 2 cut(s) 574, 961
Fnu4HI GCNGC 7 cut(s) 62, 74, 702, 705, 848, 899, 902
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 7 cut(s) 62, 74, 702, 705, 848, 899, 902
FspBI CTAG 2 cut(s) 539, 647
GluI GCNGC 7 cut(s) 62, 74, 702, 705, 848, 899, 902
HaeIII GGCC 3 cut(s) 85, 146, 565
HgaI GACGC 2 cut(s) 428, 553
Hin1II CATG 2 cut(s) 578, 965
HincII GTYRAC 1 cut(s) 443
HindII GTYRAC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 804
HinfI GANTC 3 cut(s) 662, 722, 940
HphI GGTGA 2 cut(s) 341, 745
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 1 cut(s) 793
Hpy188III TCNNGA 4 cut(s) 449, 647, 749, 962
Hpy8I GTNNAC 1 cut(s) 443
Hpy99I CGWCG 2 cut(s) 82, 166
HpyAV CCTTC 2 cut(s) 136, 500
HpyCH4IV ACGT 2 cut(s) 775, 967
HpyCH4V TGCA 8 cut(s) 359, 404, 515, 643, 704, 815, 886, 901
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 82, 410, 419, 512, 544, 812
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 775, 967
Hsp92II CATG 2 cut(s) 578, 965
Kzo9I GATC 4 cut(s) 229, 346, 430, 891
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 6 cut(s) 73, 85, 688, 716, 885, 913
LweI GCATC 4 cut(s) 28, 652, 716, 824
MaeI CTAG 2 cut(s) 539, 647
MaeII ACGT 2 cut(s) 775, 967
MaeIII GTNAC 2 cut(s) 376, 723
MalI GATC 4 cut(s) 231, 348, 432, 893
MboI GATC 4 cut(s) 229, 346, 430, 891
MboII GAAGA 9 cut(s) 46, 49, 79, 292, 440, 530, 615, 698, 939
MfeI CAATTG 1 cut(s) 810
MhlI GDGCHC 1 cut(s) 789
MluCI AATT 4 cut(s) 4, 554, 810, 948
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 2 cut(s) 238, 699
MseI TTAA 2 cut(s) 557, 761
MslI CAYNNNNRTG 1 cut(s) 354
MspR9I CCNGG 3 cut(s) 261, 341, 532
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 3 cut(s) 261, 341, 532
MwoI GCNNNNNNNGC 7 cut(s) 70, 82, 410, 419, 512, 544, 812
NdeII GATC 4 cut(s) 229, 346, 430, 891
NlaIII CATG 2 cut(s) 578, 965
NlaIV GGNNCC 2 cut(s) 159, 612
NmuCI GTSAC 2 cut(s) 376, 723
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 961
PceI AGGCCT 1 cut(s) 85
PcsI WCGNNNNNNNCGW 1 cut(s) 964
PfeI GAWTC 2 cut(s) 662, 940
PflFI GACNNNGTC 1 cut(s) 438
PfoI TCCNGGA 1 cut(s) 530
PkrI GCNGC 7 cut(s) 63, 75, 703, 706, 849, 900, 903
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
PpuMI RGGWCCY 1 cut(s) 157
Psp124BI GAGCTC 1 cut(s) 789
Psp1406I AACGTT 1 cut(s) 967
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 3 cut(s) 259, 339, 530
PspGI CCWGG 3 cut(s) 259, 339, 530
PspN4I GGNNCC 2 cut(s) 159, 612
PspPI GGNCC 3 cut(s) 145, 157, 590
PspPPI RGGWCCY 1 cut(s) 157
PspXI VCTCGAGB 1 cut(s) 149
PstI CTGCAG 2 cut(s) 706, 903
PsyI GACNNNGTC 1 cut(s) 438
RsaI GTAC 3 cut(s) 225, 254, 864
RsaNI GTAC 3 cut(s) 224, 253, 863
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 789
SaqAI TTAA 2 cut(s) 557, 761
SatI GCNGC 7 cut(s) 62, 74, 702, 705, 848, 899, 902
Sau3AI GATC 4 cut(s) 229, 346, 430, 891
Sau96I GGNCC 3 cut(s) 145, 157, 590
ScaI AGTACT 1 cut(s) 864
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 3 cut(s) 261, 341, 532
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 4 cut(s) 28, 652, 716, 824
SfcI CTRYAG 4 cut(s) 323, 600, 702, 899
Sfr274I CTCGAG 1 cut(s) 149
SinI GGWCC 2 cut(s) 157, 590
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 4 cut(s) 4, 554, 810, 948
SseBI AGGCCT 1 cut(s) 85
SsiI CCGC 2 cut(s) 422, 848
SspMI CTAG 2 cut(s) 539, 647
SstI GAGCTC 1 cut(s) 789
StuI AGGCCT 1 cut(s) 85
StyD4I CCNGG 3 cut(s) 259, 339, 530
TaiI ACGT 2 cut(s) 778, 970
TaqI TCGA 2 cut(s) 150, 433
TaqII GACCGA 1 cut(s) 674
TasI AATT 4 cut(s) 4, 554, 810, 948
TatI WGTACW 1 cut(s) 862
TauI GCSGC 1 cut(s) 850
TfiI GAWTC 2 cut(s) 662, 940
Tru1I TTAA 2 cut(s) 557, 761
Tru9I TTAA 2 cut(s) 557, 761
TscAI CASTG 2 cut(s) 634, 717
TseFI GTSAC 2 cut(s) 376, 723
TseI GCWGC 6 cut(s) 61, 73, 701, 704, 898, 901
Tsp45I GTSAC 2 cut(s) 376, 723
TspDTI ATGAA 3 cut(s) 17, 212, 810
TspRI CASTG 2 cut(s) 634, 717
Tth111I GACNNNGTC 1 cut(s) 438
VpaK11BI GGWCC 2 cut(s) 157, 590
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 2 cut(s) 4, 948
XbaI TCTAGA 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 149
XspI CTAG 2 cut(s) 539, 647
ZrmI AGTACT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.