AT2G27285

Coiled-coil domain-containing protein 55 (DUF2040)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008231
Physical Location & Seq
Reverse (-)
11675814 .. 11678118
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27285.1

Sequence Viewer

Length: 972 bp
ATGAAGAAGTACGGATTGCAAATCAGAGCTCCTTCCCAGAAAAAACAATCATCATCTCGACCTCCACTTCGACCCGCTTCAATATTTGATGAAGAAGAAGATCACGACGTCGAGAAGGAGATTTCTCGTCAAGCAACAAAGACCAAAGCTCATAAAGAAATTGAGGAGCAGCATAAGAAAGCCTTAGAGGAGGATCCTTCTGCTTTTTCATATGATGAAGTTTATGATGATATGAAACAGAAAGCTGTTCTTCCTCGAATGCAAGATCGTGAAGAACGCAAGCCAAGGTATATACAGAATTTGATGAAACAGGCAGAACGTAGAGAGAAAGAACATGAGATAGTTTATGAGAGAAAGCTTGCGAAAGAGAGGGAGAAAGACGAACATCTTTTTTCGGATAAAGAAAAGTTTGTTACTGGCGCTTATAAAAGGAAACTTGAGGAACAAAAGAAATGGCTGGCAGAAGAAAGATTGCGTGAACTTCGTGAGGAAAGAGATGATGTTACTAAGAAGAAAGATTTGAGTGATTTCTACTTCAACATTGGAAAAAATGTCGCTTTTGGAGCTCGAGAAGTCGAAGCTAAAGAGGCAGAGAAGCTCGAGGAACAAAGAAAGGCAGAGAAGCTCGAGGAACAAAGAAAGGCAGAGAAGCTCGAGGAGCTGAGAAAAGAAGTAACAAGGGTAGAGAAGAAACGAAAATCACCGGAGAAGGAAGTATCTCCTGACTCGGGAGAATTTGGATCAAGTCGTAGCAAAAGTTTGGAGCCACTAGAAGCAGAGCAAGCAGTTTCTGAAAAGGAGATGGGTTCAGATGGCACTGAAGAGAGAAAGTCATCAATCAAAGAGGCAGCAAAAGAAGTGCCGAAAGCCATTAACGACCAGAAGAGAAGAGAGGATGCGATCGCTGCCGCTAAAGAGAGGTTCCTGGCCCGTAAGAAGGCAAAAATTGAAGAGTATGTGCAACTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2040

Protein Analysis

323

Amino Acids

38.08

Weight (kDa)

8.28

Isoelectric Point (pI)

62.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSRP1_N PF09745 55 - 171 5.5e-39 Nuclear speckle splicing regulatory protein 1, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AatII GACGTC 1 cut(s) 111
AciI CCGC 2 cut(s) 75, 909
AclWI GGATC 3 cut(s) 188, 201, 748
AcsI RAATTY 2 cut(s) 298, 734
AcuI CTGAAG 1 cut(s) 840
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 728, 937
AgsI TTSAA 3 cut(s) 81, 538, 950
AjnI CCWGG 1 cut(s) 924
Alw21I GWGCWC 2 cut(s) 31, 568
AlwI GGATC 3 cut(s) 188, 201, 748
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 5 cut(s) 567, 599, 626, 653, 727
AoxI GGCC 1 cut(s) 927
ApeKI GCWGC 3 cut(s) 169, 848, 905
ApoI RAATTY 2 cut(s) 298, 734
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AsiSI GCGATCGC 1 cut(s) 903
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 928
AsuHPI GGTGA 1 cut(s) 693
AvaI CYCGRG 5 cut(s) 567, 599, 626, 653, 727
BamHI GGATCC 1 cut(s) 193
BanII GRGCYC 2 cut(s) 31, 568
Bbv12I GWGCWC 2 cut(s) 31, 568
BbvI GCAGC 3 cut(s) 181, 860, 892
BccI CCATC 2 cut(s) 796, 806
BciT130I CCWGG 1 cut(s) 926
BfaI CTAG 1 cut(s) 770
BfoI RGCGCY 1 cut(s) 423
BisI GCNGC 4 cut(s) 170, 849, 906, 909
BlsI GCNGC 4 cut(s) 171, 850, 907, 910
Bme1390I CCNGG 1 cut(s) 926
BmeT110I CYCGRG 5 cut(s) 567, 599, 626, 653, 727
BmgT120I GGNCC 1 cut(s) 928
BmiI GGNNCC 3 cut(s) 195, 765, 923
BmrFI CCNGG 1 cut(s) 926
BmsI GCATC 1 cut(s) 886
BpuEI CTTGAG 1 cut(s) 458
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 284
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 2 cut(s) 728, 937
Bse1I ACTGG 1 cut(s) 421
BseBI CCWGG 1 cut(s) 926
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 1 cut(s) 901
BseLI CCNNNNNNNGG 2 cut(s) 728, 937
BseMII CTCAG 1 cut(s) 653
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 3 cut(s) 179, 203, 671
BseXI GCAGC 3 cut(s) 181, 860, 892
Bsh1285I CGRYCG 1 cut(s) 903
BshFI GGCC 1 cut(s) 929
BsiEI CGRYCG 1 cut(s) 903
BsiHKAI GWGCWC 2 cut(s) 31, 568
BsiHKCI CYCGRG 5 cut(s) 567, 599, 626, 653, 727
BsiSI CCGG 1 cut(s) 704
BslI CCNNNNNNNGG 2 cut(s) 728, 937
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 1 cut(s) 929
BsoBI CYCGRG 5 cut(s) 567, 599, 626, 653, 727
Bsp1286I GDGCHC 2 cut(s) 31, 568
Bsp143I GATC 5 cut(s) 100, 193, 265, 740, 900
BspACI CCGC 2 cut(s) 75, 909
BspANI GGCC 1 cut(s) 929
BspCNI CTCAG 1 cut(s) 654
BspLI GGNNCC 3 cut(s) 195, 765, 923
BspPI GGATC 3 cut(s) 188, 201, 748
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 5 cut(s) 100, 193, 265, 740, 900
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 284
Bst2UI CCWGG 1 cut(s) 926
Bst6I CTCTTC 4 cut(s) 816, 878, 883, 945
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 4 cut(s) 281, 360, 459, 783
BstDEI CTNAG 3 cut(s) 184, 507, 662
BstF5I GGATG 1 cut(s) 901
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 5 cut(s) 103, 196, 268, 743, 903
BstMBI GATC 5 cut(s) 100, 193, 265, 740, 900
BstMCI CGRYCG 1 cut(s) 903
BstMWI GCNNNNNNNGC 5 cut(s) 563, 587, 658, 782, 905
BstNI CCWGG 1 cut(s) 926
BstSCI CCNGG 1 cut(s) 924
BstV1I GCAGC 3 cut(s) 181, 860, 892
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuRI GGCC 1 cut(s) 929
BtsCI GGATG 1 cut(s) 901
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 4 cut(s) 281, 360, 459, 783
CaiI CAGNNNCTG 1 cut(s) 791
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 928
Csp6I GTAC 1 cut(s) 10
CviAII CATG 1 cut(s) 335
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 3 cut(s) 184, 507, 662
DpnI GATC 5 cut(s) 102, 195, 267, 742, 902
DpnII GATC 5 cut(s) 100, 193, 265, 740, 900
Eam1104I CTCTTC 4 cut(s) 816, 878, 883, 945
EarI CTCTTC 4 cut(s) 816, 878, 883, 945
Ecl136II GAGCTC 2 cut(s) 29, 566
Eco130I CCWWGG 1 cut(s) 284
Eco24I GRGCYC 2 cut(s) 31, 568
Eco53kI GAGCTC 2 cut(s) 29, 566
Eco57I CTGAAG 1 cut(s) 840
Eco88I CYCGRG 5 cut(s) 567, 599, 626, 653, 727
EcoICRI GAGCTC 2 cut(s) 29, 566
EcoRII CCWGG 1 cut(s) 924
EcoT14I CCWWGG 1 cut(s) 284
EcoT38I GRGCYC 2 cut(s) 31, 568
ErhI CCWWGG 1 cut(s) 284
FaeI CATG 1 cut(s) 338
FalI AAGNNNNNCTT 4 cut(s) 167, 199, 234, 266
FatI CATG 1 cut(s) 334
FauI CCCGC 1 cut(s) 82
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 4 cut(s) 170, 849, 906, 909
FokI GGATG 1 cut(s) 908
FriOI GRGCYC 2 cut(s) 31, 568
Fsp4HI GCNGC 4 cut(s) 170, 849, 906, 909
FspBI CTAG 1 cut(s) 770
GlaI GCGC 1 cut(s) 421
GluI GCNGC 4 cut(s) 170, 849, 906, 909
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 1 cut(s) 929
HapII CCGG 1 cut(s) 704
HhaI GCGC 1 cut(s) 422
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 1 cut(s) 338
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 1 cut(s) 725
HpaII CCGG 1 cut(s) 704
HphI GGTGA 1 cut(s) 693
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 4 cut(s) 26, 397, 793, 811
Hpy188III TCNNGA 8 cut(s) 57, 104, 112, 269, 485, 569, 722, 729
Hpy8I GTNNAC 1 cut(s) 479
Hpy99I CGWCG 2 cut(s) 110, 113
HpyAV CCTTC 5 cut(s) 42, 109, 207, 703, 931
HpyCH4IV ACGT 2 cut(s) 108, 319
HpyCH4V TGCA 3 cut(s) 19, 262, 961
HpyF10VI GCNNNNNNNGC 5 cut(s) 563, 587, 658, 782, 905
HpyF3I CTNAG 3 cut(s) 184, 507, 662
HpySE526I ACGT 2 cut(s) 108, 319
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 338
HspAI GCGC 1 cut(s) 420
Kzo9I GATC 5 cut(s) 100, 193, 265, 740, 900
LmnI GCTCC 5 cut(s) 34, 166, 563, 658, 763
LpnPI CCDG 9 cut(s) 50, 296, 402, 443, 717, 735, 893, 911, 938
Lsp1109I GCAGC 3 cut(s) 181, 860, 892
LweI GCATC 1 cut(s) 886
MaeI CTAG 1 cut(s) 770
MaeII ACGT 2 cut(s) 108, 319
MaeIII GTNAC 3 cut(s) 412, 502, 673
MalI GATC 5 cut(s) 102, 195, 267, 742, 902
MboI GATC 5 cut(s) 100, 193, 265, 740, 900
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 31, 568
MluCI AATT 4 cut(s) 159, 298, 734, 945
MlyI GAGTC 1 cut(s) 719
MseI TTAA 1 cut(s) 873
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 1 cut(s) 926
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 1 cut(s) 926
MwoI GCNNNNNNNGC 5 cut(s) 563, 587, 658, 782, 905
NdeI CATATG 1 cut(s) 211
NdeII GATC 5 cut(s) 100, 193, 265, 740, 900
NlaIII CATG 1 cut(s) 338
NlaIV GGNNCC 3 cut(s) 195, 765, 923
PaeR7I CTCGAG 4 cut(s) 567, 599, 626, 653
PctI GAATGC 1 cut(s) 264
PkrI GCNGC 4 cut(s) 171, 850, 907, 910
Ple19I CGATCG 1 cut(s) 903
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
PsiI TTATAA 1 cut(s) 426
Psp124BI GAGCTC 2 cut(s) 31, 568
Psp6I CCWGG 1 cut(s) 924
PspGI CCWGG 1 cut(s) 924
PspN4I GGNNCC 3 cut(s) 195, 765, 923
PspPI GGNCC 1 cut(s) 928
PspXI VCTCGAGB 3 cut(s) 599, 626, 653
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 1 cut(s) 193
PvuI CGATCG 1 cut(s) 903
RgaI GCGATCGC 1 cut(s) 903
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SacI GAGCTC 2 cut(s) 31, 568
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 4 cut(s) 170, 849, 906, 909
Sau3AI GATC 5 cut(s) 100, 193, 265, 740, 900
Sau96I GGNCC 1 cut(s) 928
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 926
SduI GDGCHC 2 cut(s) 31, 568
SfaAI GCGATCGC 1 cut(s) 903
SfaNI GCATC 1 cut(s) 886
Sfr274I CTCGAG 4 cut(s) 567, 599, 626, 653
SgfI GCGATCGC 1 cut(s) 903
SlaI CTCGAG 4 cut(s) 567, 599, 626, 653
SmlI CTYRAG 5 cut(s) 437, 567, 599, 626, 653
SmoI CTYRAG 5 cut(s) 437, 567, 599, 626, 653
Sse9I AATT 4 cut(s) 159, 298, 734, 945
SsiI CCGC 2 cut(s) 75, 909
SspI AATATT 1 cut(s) 84
SspMI CTAG 1 cut(s) 770
SstI GAGCTC 2 cut(s) 31, 568
StyD4I CCNGG 1 cut(s) 924
StyI CCWWGG 1 cut(s) 284
TaiI ACGT 2 cut(s) 111, 322
TaqI TCGA 9 cut(s) 58, 70, 111, 256, 568, 576, 600, 627, 654
TasI AATT 4 cut(s) 159, 298, 734, 945
TauI GCSGC 1 cut(s) 911
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 1 cut(s) 823
TseI GCWGC 3 cut(s) 169, 848, 905
TspDTI ATGAA 6 cut(s) 17, 105, 198, 231, 248, 320
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 823
XapI RAATTY 2 cut(s) 298, 734
XhoI CTCGAG 4 cut(s) 567, 599, 626, 653
XspI CTAG 1 cut(s) 770
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.