MD01G1023000.v1.1

Nuclear speckle splicing regulatory protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008231
Physical Location & Seq
Forward (+)
9038259 .. 9038612
354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1023000.v1.1.491

Sequence Viewer

Length: 354 bp
ATGAATTTGGAAGCAGAGCATCACAGGAAAAAGAAGAAGACGAGACGAAGAATGATTAAGTACGGGTTGAACCTAAGGGGGCAGCAGAAGAAGCAGCCGACGAGGCCTCCTCTCCCTAAACCTCGGGGTTTTGGCGATGACAACAATGACAATGATGTTGAGAAGGAAATAAGTCGGCAGGCTTCCAAGAACGAGTCTCTGAAAGATATTGAGGAGCAACAGAGGAAGGCCCTGGAGCAGGACCAGTCAGTGTTCGACTACGATGGAGTTTATGATGAGATGAAACAGAAAGCCATCCAACCTCAGGTTCAAGATCGCCAAGAGAGAATGGCAAGGTATATTCCTGGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2040

Protein Analysis

118

Amino Acids

13.91

Weight (kDa)

9.58

Isoelectric Point (pI)

62.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSRP1_N PF09745 71 - 115 2.6e-10 Nuclear speckle splicing regulatory protein 1, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 238, 304
AgsI TTSAA 2 cut(s) 70, 311
AjnI CCWGG 2 cut(s) 231, 343
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
Alw26I GTCTC 2 cut(s) 37, 201
Ama87I CYCGRG 1 cut(s) 123
AoxI GGCC 2 cut(s) 104, 228
ApeKI GCWGC 2 cut(s) 82, 94
ApoI RAATTY 1 cut(s) 4
AspS9I GGNCC 2 cut(s) 229, 241
AvaI CYCGRG 1 cut(s) 123
AvaII GGWCC 1 cut(s) 241
AxyI CCTNAGG 2 cut(s) 74, 303
BbsI GAAGAC 1 cut(s) 44
BbvI GCAGC 2 cut(s) 94, 106
BccI CCATC 2 cut(s) 257, 302
BciT130I CCWGG 2 cut(s) 233, 345
BcoDI GTCTC 2 cut(s) 37, 201
BglI GCCNNNNNGGC 1 cut(s) 103
BisI GCNGC 2 cut(s) 83, 95
BlsI GCNGC 2 cut(s) 84, 96
Bme1390I CCNGG 2 cut(s) 233, 345
Bme18I GGWCC 1 cut(s) 241
BmeT110I CYCGRG 1 cut(s) 123
BmgT120I GGNCC 2 cut(s) 229, 241
BmrFI CCNGG 2 cut(s) 233, 345
BmsI GCATC 1 cut(s) 28
BpiI GAAGAC 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 94, 126
BpmI CTGGAG 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 122, 231
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 304
Bse1I ACTGG 1 cut(s) 244
Bse21I CCTNAGG 2 cut(s) 74, 303
BseBI CCWGG 2 cut(s) 233, 345
BseDI CCNNGG 2 cut(s) 122, 231
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 2 cut(s) 238, 304
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 2 cut(s) 99, 227
BseXI GCAGC 2 cut(s) 94, 106
BshFI GGCC 2 cut(s) 106, 230
BsiHKCI CYCGRG 1 cut(s) 123
BslI CCNNNNNNNGG 2 cut(s) 238, 304
BsmAI GTCTC 2 cut(s) 37, 201
BsmBI CGTCTC 1 cut(s) 37
BsnI GGCC 2 cut(s) 106, 230
BsoBI CYCGRG 1 cut(s) 123
Bsp143I GATC 1 cut(s) 313
BspANI GGCC 2 cut(s) 106, 230
BspCNI CTCAG 1 cut(s) 316
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 122, 231
BssMI GATC 1 cut(s) 313
Bst2UI CCWGG 2 cut(s) 233, 345
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 2 cut(s) 74, 303
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 1 cut(s) 316
BstMAI GTCTC 2 cut(s) 37, 201
BstMBI GATC 1 cut(s) 313
BstMWI GCNNNNNNNGC 2 cut(s) 91, 103
BstNI CCWGG 2 cut(s) 233, 345
BstSCI CCNGG 2 cut(s) 231, 343
BstV1I GCAGC 2 cut(s) 94, 106
BstV2I GAAGAC 1 cut(s) 44
Bsu36I CCTNAGG 2 cut(s) 74, 303
BsuRI GGCC 2 cut(s) 106, 230
BtgZI GCGATG 1 cut(s) 150
BtsCI GGATG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 229, 241
Csp6I GTAC 1 cut(s) 61
CviJI RGCY 5 cut(s) 97, 106, 182, 230, 293
CviKI_1 RGCY 5 cut(s) 97, 106, 182, 230, 293
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 2 cut(s) 74, 303
DpnI GATC 1 cut(s) 315
DpnII GATC 1 cut(s) 313
Eco147I AGGCCT 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 241
Eco81I CCTNAGG 2 cut(s) 74, 303
Eco88I CYCGRG 1 cut(s) 123
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 2 cut(s) 231, 343
Esp3I CGTCTC 1 cut(s) 37
FaiI YATR 4 cut(s) 273, 339, 350, 352
Fnu4HI GCNGC 2 cut(s) 83, 95
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 83, 95
GluI GCNGC 2 cut(s) 83, 95
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 2 cut(s) 106, 230
HinfI GANTC 1 cut(s) 194
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 311
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 157, 220
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 103
HpyF3I CTNAG 2 cut(s) 74, 303
Kzo9I GATC 1 cut(s) 313
LmnI GCTCC 2 cut(s) 214, 235
LpnPI CCDG 8 cut(s) 10, 164, 218, 224, 245, 257, 290, 330
Lsp1109I GCAGC 2 cut(s) 94, 106
LweI GCATC 1 cut(s) 28
MalI GATC 1 cut(s) 315
MboI GATC 1 cut(s) 313
MboII GAAGA 4 cut(s) 46, 49, 60, 100
MluCI AATT 1 cut(s) 4
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 322
MnlI CCTC 7 cut(s) 96, 117, 120, 132, 205, 216, 312
MseI TTAA 1 cut(s) 57
MspR9I CCNGG 2 cut(s) 233, 345
MvaI CCWGG 2 cut(s) 233, 345
MwoI GCNNNNNNNGC 2 cut(s) 91, 103
NdeII GATC 1 cut(s) 313
PceI AGGCCT 1 cut(s) 106
PkrI GCNGC 2 cut(s) 84, 96
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 231, 343
PspGI CCWGG 2 cut(s) 231, 343
PspPI GGNCC 2 cut(s) 229, 241
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 2 cut(s) 83, 95
Sau3AI GATC 1 cut(s) 313
Sau96I GGNCC 2 cut(s) 229, 241
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 233, 345
SetI ASST 5 cut(s) 75, 124, 304, 309, 338
SfaNI GCATC 1 cut(s) 28
SinI GGWCC 1 cut(s) 241
Sse9I AATT 1 cut(s) 4
SseBI AGGCCT 1 cut(s) 106
StuI AGGCCT 1 cut(s) 106
StyD4I CCNGG 2 cut(s) 231, 343
TaqI TCGA 1 cut(s) 255
TasI AATT 1 cut(s) 4
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TscAI CASTG 1 cut(s) 255
TseI GCWGC 2 cut(s) 82, 94
TspDTI ATGAA 2 cut(s) 17, 296
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 241
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 1 cut(s) 4
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.