Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002975

Physical Location & Seq

Genomic Coordinates Forward (+)

13953665 .. 13957748

Gene Structure 4084 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G32900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGCCGGAGATAGACGCGTTGTTTGAATCGATCAATGTTCGGGACCTTCTCGCCGGACACGATCTAAACGATCCCACAACTCCGTTATCTGCACCGGATCTCCGTCTCCTCATAAACCGCTTAGAGTCTCATTCTCTGCGGATCAAGTCCAAAGTTCAGTCTTATCTCGTCGCTCATCACTCTGATTTCTCGGAGCTATTTTCTCTTTGTCAAGACACCGTCTCGAGGACTCGTCTGATCTCCGATGACGTCTCCGATGTCCTACAATTGGTTTCTGACCGTCCGATTGATGTTGAGATACGCAGTGTGGTAGATGAGATAACGGAAAAGACTAAGGAAGTGAAATTGAAAAGAGAATCTCTTGATTTAGTGAATGCGATCGTGGGAATTTGTGAGGCATTGCAGGAAACGAAGGAAGCTTTGAAAAATGGACGTTTCAGATTCGCCGCTGAGAGAATCAGAGAACTTAAAGTTGTATTGAGAATTGGTGAAGAGGAAGATGGAGAACCTGTGGCTTACGCTTTACTTAGGAAGGAATGGTCTAATTGCTTTGACGAGATTCAAGAGGTGCTTGCAAAGTTCATGGAGAATGCAGTGCGTTTTGAGCTAGACTCGAGTAGAATCCGGATTAAGTATCAGCTAAGCGTTGGTGAGACTGCTGGAATCGCTCTTAGTACTGTTCTAGAGGCAATGGAGGTGATTGGAATATTAGACTATGGGCTTGCGAAAGCAGCTGACTCAATCTTTAAGCATGTCATCACTCCAGCTGTAACTCATGCATCTACCTTTGCCGCTGTGGAAGATTTATGTAAATCCGCAGGGGAAGTCACCGAAGCGACACTAAGGCTAGAGCAATCCTCAGATCATAAGTTTGAAGATGTGGATGGAGATGCAATGTATTCTGGAATTCTTAAGGTTGTTAAGTTCATTTGCAGTTCCTTGTGCTTTGGAAACGTTACATGGATCCATTCTTTTGGGAGGTTGACATGGCCGAGGATATCAGAGTTAATTATATCTAAATTTCTTTCAAAGGTTGTCCCAGAAGATGCTTCAAAACTTGCTGACTTTCAGAAAATTATCGAGCGGACCTCTCAATTCGAAGCCGCTCTAAAGGAACTGAATTTTGTTTCATCATCTGACGCAGAGAGTAGGCTCAGCAAATATGCTGAAGATGTGGAGGTTCATTTTGCATCTAGAAAGAAGATAGAAATCCTAGCAAAAGCAAGAAATTTGCTGTTGCAGTGTAACTTTACGATTCCCCAGGATATTGCTATGAGGAATGCCAAGCACATCGTCTGCTTACTTTTCTCCTCTGAAAGGTGTGTGGTATCTGAAGCTGCATCTCAACTTATGAATTTGGTGCATAAGACGCTCGAGGATGTCTGTGTTTCTTCTGCAAGGGTTGCTTCAGAGTTCTACAACGCTGCTAGAGATTCTATCCTTCTATATGAAGCTGTTGTTCCTGTCAAGCTAGAGAAACAACTCGATGGCCTCAATGAAGCTGCTGTTCTTCTGCACAACGATTGTCTATATTTGTTCGAAGAGATACTTGGCCTTGCTTTTGAGTATCGTGCTAGCTTCCCTAGCTCCATCAAGGAGTATGCAGTCTTTGCTGACATAGCCCCAAGATTTAAACTGATGGCCGAAGAAGTTTTGCAAAAACAAGTTCATCTAGTAATTTCAAGTCTGCGAGAGGCTATAGATAGTGCAGATGGATTTCAGAATACTCATCAAATTAAACAGTTTAAATCTGCAGAATTTAGCATTGATCAGGTTGTCTTTAGTCTAAAGAATGTGCATATGATATGGGAGCCAGTCTTACGGCCCAAAACTTACAAGCAAAGCATGTGCGCAGTTTTGGAGTCTGTATTTCGCAGAATAGCTAGAGATATTCTTCTTCTAGACGACATGGCTGCTGACGAAACTTTCGAGCTACAAAAACTGATTTATCTCATGTTGAAGAATCTATCTTCTGTACTCGATTCCGTGAGATCTGCAGATGAGACTTCTCGTCCTCTTGATGATATCATACCATCTTTGCGCAAAACCCGTAAACTAGCAGAACTATTGGATATGCCTCTCATGTCTATAACTTCAGCTTGGGAAAGCGGGGAGTTATTTAGATGCAATTTCACAAGAACAGAGGTACAAGATTTCATCAAAGCTATATTTACAGATTCGCCGCTGAGAAAAGAATGCTTATGGAGGATAGACGAGGTTAACCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000278 GO:0000775 GO:0000776 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006810 GO:0006888 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0016192 GO:0022402 GO:0030071 GO:0031577 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048193 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0098687 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:1990423 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Zw10

Protein Analysis

742

Amino Acids

83.93

Weight (kDa)

5.2

Isoelectric Point (pI)

41.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zw10_N	PF06248	41 - 134	2e-28	Centromere/kinetochore Zw10 N-terminal
Zw10_middle	PF20665	176 - 412	7.6e-80	Centromere/kinetochore protein zw10, middle domain
ZW10_C	PF20666	436 - 565	4.1e-49	Centromere/kinetochore protein zw10, C-terminal
ZW10_C2	PF22766	588 - 737	2.5e-57	ZW10 C-terminal helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32900
fragaria_vesca	FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900
malus_domestica	MD09G1116200.v1.1 MD17G1107900.v1.1
prunus_persica	Prupe.3G208500_v2.0.a1
pyrus_communis	pycom09g04060 pycom17g10050
rosa_chinensis	RchiOBHm_Chr0c20g0500271 RchiOBHm_Chr1g0340131 RchiOBHm_Chr1g0340141 RchiOBHm_Chr2g0157781
rosa_laevigata	RLG00000021018 RLG00000028993
rosa_multiflora	Rmu_co8468091.1_g000001 Rmu_sc0001275.1_g000033 Rmu_sc0010800.1_g000004 Rmu_sc0018661.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091960 Rroxscaffold_4G00311000
rosa_rugosa	Rorug02G0463300 Rorug02G0463300
rosa_samantha	Rh1AG180900 Rh1CG167700 Rh1DG181000 Rh1DG181100 Rh2AG528800 Rh2BG542700 Rh2CG513100 Rh2DG551700
rosa_wichuraiana	Rw1G015020 Rw2G043720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	252
Acc16I	TGCGCA	2 cut(s)	1852, 2042
AccBSI	CCGCTC	2 cut(s)	1084, 1106
AccII	CGCG	1 cut(s)	17
AccIII	TCCGGA	1 cut(s)	624
AciI	CCGC	9 cut(s)	118, 139, 447, 792, 816, 1084, 1104, 2109, 2183
AclI	AACGTT	1 cut(s)	954
AclWI	GGATC	5 cut(s)	65, 105, 149, 958, 971
AcoI	YGGCCR	2 cut(s)	989, 1641
AcsI	RAATTY	7 cut(s)	387, 906, 1019, 1120, 1228, 1354, 1757
AcuI	CTGAAG	4 cut(s)	1188, 1353, 1392, 2079
AcyI	GRCGYC	1 cut(s)	249
AfaI	GTAC	3 cut(s)	676, 1977, 2148
AfiI	CCNNNNNNNGG	3 cut(s)	225, 268, 1317
AflII	CTTAAG	1 cut(s)	911
AflIII	ACRYGT	1 cut(s)	15
AgsI	TTSAA	9 cut(s)	26, 349, 424, 563, 875, 1029, 1053, 1683, 1960
AhdI	GACNNNNNGTC	1 cut(s)	2010
AjnI	CCWGG	1 cut(s)	1260
AjuI	GAANNNNNNNTTGG	2 cut(s)	1533, 1565
Alw26I	GTCTC	6 cut(s)	110, 132, 226, 256, 647, 1997
AlwI	GGATC	5 cut(s)	65, 105, 149, 958, 971
Ama87I	CYCGRG	3 cut(s)	223, 613, 1373
Aor13HI	TCCGGA	1 cut(s)	624
AoxI	GGCC	5 cut(s)	989, 1489, 1552, 1641, 1823
ApeKI	GCWGC	5 cut(s)	731, 1337, 1424, 1502, 1913
ApoI	RAATTY	7 cut(s)	387, 906, 1019, 1120, 1228, 1354, 1757
ArsI	GACNNNNNNTTYG	3 cut(s)	37, 1910, 1942
AspLEI	GCGC	2 cut(s)	1853, 2043
AspS9I	GGNCC	3 cut(s)	43, 1086, 1824
AsuHPI	GGTGA	4 cut(s)	500, 662, 709, 820
AsuII	TTCGAA	2 cut(s)	1098, 1539
AsuNHI	GCTAGC	1 cut(s)	1574
AvaI	CYCGRG	3 cut(s)	223, 613, 1373
AvaII	GGWCC	2 cut(s)	43, 1086
BamHI	GGATCC	1 cut(s)	963
BbvI	GCAGC	5 cut(s)	743, 1324, 1411, 1489, 1900
BccI	CCATC	7 cut(s)	494, 878, 1481, 1598, 1633, 1706, 2041
BceAI	ACGGC	1 cut(s)	1838
BcgI	CGANNNNNNTGC	2 cut(s)	1895, 1929
BciT130I	CCWGG	1 cut(s)	1262
BclI	TGATCA	1 cut(s)	1768
BcoDI	GTCTC	6 cut(s)	110, 132, 226, 256, 647, 1997
BfmI	CTRYAG	3 cut(s)	1698, 1752, 1995
BfrI	CTTAAG	1 cut(s)	911
BglII	AGATCT	1 cut(s)	1991
BisI	GCNGC	9 cut(s)	447, 732, 792, 1104, 1338, 1425, 1503, 1914, 2183
BlpI	GCTNAGC	2 cut(s)	641, 1154
BlsI	GCNGC	9 cut(s)	448, 733, 793, 1105, 1339, 1426, 1504, 1915, 2184
BmcAI	AGTACT	1 cut(s)	676
Bme1390I	CCNGG	1 cut(s)	1262
Bme18I	GGWCC	2 cut(s)	43, 1086
BmeRI	GACNNNNNGTC	1 cut(s)	2010
BmeT110I	CYCGRG	3 cut(s)	223, 613, 1373
BmgT120I	GGNCC	3 cut(s)	43, 1086, 1824
BmiI	GGNNCC	3 cut(s)	44, 965, 1812
BmrFI	CCNGG	1 cut(s)	1262
BmsI	GCATC	6 cut(s)	788, 880, 1036, 1199, 1349, 2114
BmtI	GCTAGC	1 cut(s)	1578
BplI	GAGNNNNNCTC	6 cut(s)	596, 628, 842, 874, 1073, 1105
BpmI	CTGGAG	1 cut(s)	747
Bpu1102I	GCTNAGC	2 cut(s)	641, 1154
Bpu14I	TTCGAA	2 cut(s)	1098, 1539
Bsa29I	ATCGAT	1 cut(s)	29
BsaBI	GATNNNNATC	1 cut(s)	1208
BsaHI	GRCGYC	1 cut(s)	249
BsaJI	CCNNGG	2 cut(s)	992, 1260
BsaWI	WCCGGW	2 cut(s)	94, 624
BsaXI	ACNNNNNCTCC	4 cut(s)	84, 114, 495, 525
Bsc4I	CCNNNNNNNGG	3 cut(s)	225, 268, 1317
Bse1I	ACTGG	2 cut(s)	1814, 2224
Bse3DI	GCAATG	3 cut(s)	398, 696, 900
Bse8I	GATNNNNATC	1 cut(s)	1208
BseAI	TCCGGA	1 cut(s)	624
BseBI	CCWGG	1 cut(s)	1262
BseCI	ATCGAT	1 cut(s)	29
BseDI	CCNNGG	2 cut(s)	992, 1260
BseGI	GGATG	2 cut(s)	889, 1384
BseJI	GATNNNNATC	1 cut(s)	1208
BseLI	CCNNNNNNNGG	3 cut(s)	225, 268, 1317
BseMI	GCAATG	3 cut(s)	398, 696, 900
BseMII	CTCAG	4 cut(s)	441, 873, 1168, 2177
BseNI	ACTGG	2 cut(s)	1814, 2224
BseRI	GAGGAG	2 cut(s)	98, 1300
BseXI	GCAGC	5 cut(s)	743, 1324, 1411, 1489, 1900
BsgI	GTGCAG	3 cut(s)	75, 1499, 1728
Bsh1236I	CGCG	1 cut(s)	17
Bsh1285I	CGRYCG	1 cut(s)	381
BshFI	GGCC	5 cut(s)	991, 1491, 1554, 1643, 1825
BshVI	ATCGAT	1 cut(s)	29
BsiEI	CGRYCG	1 cut(s)	381
BsiHKCI	CYCGRG	3 cut(s)	223, 613, 1373
BsiSI	CCGG	4 cut(s)	5, 54, 95, 625
BslFI	GGGAC	2 cut(s)	56, 1022
BslI	CCNNNNNNNGG	3 cut(s)	225, 268, 1317
BsmAI	GTCTC	6 cut(s)	110, 132, 226, 256, 647, 1997
BsmBI	CGTCTC	3 cut(s)	110, 226, 256
BsmFI	GGGAC	2 cut(s)	56, 1022
BsmI	GAATGC	4 cut(s)	379, 595, 1285, 2201
BsnI	GGCC	5 cut(s)	991, 1491, 1554, 1643, 1825
BsoBI	CYCGRG	3 cut(s)	223, 613, 1373
Bsp119I	TTCGAA	2 cut(s)	1098, 1539
Bsp13I	TCCGGA	1 cut(s)	624
Bsp1720I	GCTNAGC	2 cut(s)	641, 1154
BspACI	CCGC	9 cut(s)	118, 139, 447, 792, 816, 1084, 1104, 2109, 2183
BspANI	GGCC	5 cut(s)	991, 1491, 1554, 1643, 1825
BspCNI	CTCAG	4 cut(s)	442, 872, 1167, 2178
BspDI	ATCGAT	1 cut(s)	29
BspEI	TCCGGA	1 cut(s)	624
BspFNI	CGCG	1 cut(s)	17
BspLI	GGNNCC	3 cut(s)	44, 965, 1812
BspMAI	CTGCAG	2 cut(s)	1756, 1999
BspOI	GCTAGC	1 cut(s)	1578
BspPI	GGATC	5 cut(s)	65, 105, 149, 958, 971
BspT104I	TTCGAA	2 cut(s)	1098, 1539
BspTI	CTTAAG	1 cut(s)	911
BsrBI	CCGCTC	2 cut(s)	1084, 1106
BsrDI	GCAATG	3 cut(s)	398, 696, 900
BsrI	ACTGG	2 cut(s)	1814, 2224
BssECI	CCNNGG	2 cut(s)	992, 1260
BssNI	GRCGYC	1 cut(s)	249
Bst2UI	CCWGG	1 cut(s)	1262
Bst4CI	ACNGT	4 cut(s)	220, 281, 679, 1743
Bst6I	CTCTTC	2 cut(s)	486, 1536
BstACI	GRCGYC	1 cut(s)	249
BstAFI	CTTAAG	1 cut(s)	911
BstAPI	GCANNNNNTGC	2 cut(s)	1403, 1610
BstBI	TTCGAA	2 cut(s)	1098, 1539
BstC8I	GCNNGC	3 cut(s)	573, 723, 1576
BstENI	CCTNNNNNAGG	1 cut(s)	1315
BstF5I	GGATG	2 cut(s)	889, 1384
BstFNI	CGCG	1 cut(s)	17
BstHHI	GCGC	2 cut(s)	1853, 2043
BstMAI	GTCTC	6 cut(s)	110, 132, 226, 256, 647, 1997
BstMCI	CGRYCG	1 cut(s)	381
BstMWI	GCNNNNNNNGC	8 cut(s)	604, 665, 731, 1369, 1403, 1584, 1610, 1619
BstNI	CCWGG	1 cut(s)	1262
BstNSI	RCATGY	2 cut(s)	755, 1849
BstSCI	CCNGG	1 cut(s)	1260
BstSFI	CTRYAG	3 cut(s)	1698, 1752, 1995
BstUI	CGCG	1 cut(s)	17
BstV1I	GCAGC	5 cut(s)	743, 1324, 1411, 1489, 1900
BstX2I	RGATCY	3 cut(s)	97, 963, 1991
BstXI	CCANNNNNNTGG	1 cut(s)	974
BstYI	RGATCY	3 cut(s)	97, 963, 1991
Bsu15I	ATCGAT	1 cut(s)	29
BsuRI	GGCC	5 cut(s)	991, 1491, 1554, 1643, 1825
BsuTUI	ATCGAT	1 cut(s)	29
BtsCI	GGATG	2 cut(s)	889, 1384
BtsI	GCAGTG	3 cut(s)	310, 600, 1247
BtsIMutI	CAGTG	3 cut(s)	310, 600, 1247
Cac8I	GCNNGC	3 cut(s)	573, 723, 1576
CfoI	GCGC	2 cut(s)	1853, 2043
Cfr13I	GGNCC	3 cut(s)	43, 1086, 1824
ClaI	ATCGAT	1 cut(s)	29
CseI	GACGC	3 cut(s)	23, 1148, 1378
Csp6I	GTAC	3 cut(s)	675, 1976, 2147
CviAII	CATG	9 cut(s)	583, 752, 776, 960, 987, 1846, 1909, 1954, 2083
CviQI	GTAC	3 cut(s)	675, 1976, 2147
DraI	TTTAAA	2 cut(s)	1633, 1747
DriI	GACNNNNNGTC	1 cut(s)	2010
EaeI	YGGCCR	2 cut(s)	989, 1641
Eam1104I	CTCTTC	2 cut(s)	486, 1536
Eam1105I	GACNNNNNGTC	1 cut(s)	2010
EarI	CTCTTC	2 cut(s)	486, 1536
Eco32I	GATATC	2 cut(s)	999, 2026
Eco47I	GGWCC	2 cut(s)	43, 1086
Eco57I	CTGAAG	4 cut(s)	1188, 1353, 1392, 2079
Eco88I	CYCGRG	3 cut(s)	223, 613, 1373
EcoNI	CCTNNNNNAGG	1 cut(s)	1315
EcoO109I	RGGNCCY	1 cut(s)	43
EcoRI	GAATTC	1 cut(s)	906
EcoRII	CCWGG	1 cut(s)	1260
EcoRV	GATATC	2 cut(s)	999, 2026
EcoT22I	ATGCAT	1 cut(s)	781
Esp3I	CGTCTC	3 cut(s)	110, 226, 256
FaeI	CATG	9 cut(s)	586, 755, 779, 963, 990, 1849, 1912, 1957, 2086
FalI	AAGNNNNNCTT	6 cut(s)	555, 587, 1390, 1422, 1533, 1565
FaqI	GGGAC	2 cut(s)	56, 1022
FatI	CATG	9 cut(s)	582, 751, 775, 959, 986, 1845, 1908, 1953, 2082
FauI	CCCGC	1 cut(s)	2102
FauNDI	CATATG	1 cut(s)	1800
FbaI	TGATCA	1 cut(s)	1768
Fnu4HI	GCNGC	9 cut(s)	447, 732, 792, 1104, 1338, 1425, 1503, 1914, 2183
FokI	GGATG	2 cut(s)	896, 1391
Fsp4HI	GCNGC	9 cut(s)	447, 732, 792, 1104, 1338, 1425, 1503, 1914, 2183
FspI	TGCGCA	2 cut(s)	1852, 2042
GlaI	GCGC	2 cut(s)	1852, 2042
GluI	GCNGC	9 cut(s)	447, 732, 792, 1104, 1338, 1425, 1503, 1914, 2183
GsuI	CTGGAG	1 cut(s)	747
HaeIII	GGCC	5 cut(s)	991, 1491, 1554, 1643, 1825
HapII	CCGG	4 cut(s)	5, 54, 95, 625
HgaI	GACGC	3 cut(s)	23, 1148, 1378
HhaI	GCGC	2 cut(s)	1853, 2043
Hin1I	GRCGYC	1 cut(s)	249
Hin1II	CATG	9 cut(s)	586, 755, 779, 963, 990, 1849, 1912, 1957, 2086
Hin6I	GCGC	2 cut(s)	1851, 2041
HinP1I	GCGC	2 cut(s)	1851, 2041
HincII	GTYRAC	2 cut(s)	984, 2221
HindII	GTYRAC	2 cut(s)	984, 2221
HindIII	AAGCTT	1 cut(s)	417
HpaI	GTTAAC	1 cut(s)	2221
HpaII	CCGG	4 cut(s)	5, 54, 95, 625
HphI	GGTGA	4 cut(s)	500, 662, 709, 820
Hpy166II	GTNNAC	3 cut(s)	984, 2054, 2221
Hpy8I	GTNNAC	3 cut(s)	984, 2054, 2221
Hpy99I	CGWCG	1 cut(s)	173
HpyAV	CCTTC	4 cut(s)	56, 406, 526, 1451
HpyCH4III	ACNGT	4 cut(s)	220, 281, 679, 1743
HpyCH4IV	ACGT	3 cut(s)	249, 433, 954
HpyF10VI	GCNNNNNNNGC	8 cut(s)	604, 665, 731, 1369, 1403, 1584, 1610, 1619
HpySE526I	ACGT	3 cut(s)	249, 433, 954
Hsp92I	GRCGYC	1 cut(s)	249
Hsp92II	CATG	9 cut(s)	586, 755, 779, 963, 990, 1849, 1912, 1957, 2086
HspAI	GCGC	2 cut(s)	1851, 2041
Kpn2I	TCCGGA	1 cut(s)	624
Ksp22I	TGATCA	1 cut(s)	1768
KspAI	GTTAAC	1 cut(s)	2221
LmnI	GCTCC	3 cut(s)	193, 1592, 1810
Lsp1109I	GCAGC	5 cut(s)	743, 1324, 1411, 1489, 1900
LweI	GCATC	6 cut(s)	788, 880, 1036, 1199, 1349, 2114
MaeII	ACGT	3 cut(s)	249, 433, 954
MaeIII	GTNAC	4 cut(s)	769, 826, 955, 1244
MbiI	CCGCTC	2 cut(s)	1084, 1106
MfeI	CAATTG	1 cut(s)	266
MflI	RGATCY	3 cut(s)	97, 963, 1991
MluI	ACGCGT	1 cut(s)	15
MlyI	GAGTC	5 cut(s)	134, 223, 605, 731, 1871
Mph1103I	ATGCAT	1 cut(s)	781
MroI	TCCGGA	1 cut(s)	624
MspA1I	CMGCKG	5 cut(s)	449, 734, 767, 794, 2185
MspCI	CTTAAG	1 cut(s)	911
MspI	CCGG	4 cut(s)	5, 54, 95, 625
MspR9I	CCNGG	1 cut(s)	1262
MunI	CAATTG	1 cut(s)	266
Mva1269I	GAATGC	4 cut(s)	379, 595, 1285, 2201
MvaI	CCWGG	1 cut(s)	1262
MvnI	CGCG	1 cut(s)	17
MwoI	GCNNNNNNNGC	8 cut(s)	604, 665, 731, 1369, 1403, 1584, 1610, 1619
NdeI	CATATG	1 cut(s)	1800
NheI	GCTAGC	1 cut(s)	1574
NlaIII	CATG	9 cut(s)	586, 755, 779, 963, 990, 1849, 1912, 1957, 2086
NlaIV	GGNNCC	3 cut(s)	44, 965, 1812
NmeAIII	GCCGAG	1 cut(s)	1017
NmuCI	GTSAC	1 cut(s)	826
NsbI	TGCGCA	2 cut(s)	1852, 2042
NsiI	ATGCAT	1 cut(s)	781
NspI	RCATGY	2 cut(s)	755, 1849
NspV	TTCGAA	2 cut(s)	1098, 1539
PaeR7I	CTCGAG	3 cut(s)	223, 613, 1373
PcsI	WCGNNNNNNNCGW	3 cut(s)	57, 66, 1926
PctI	GAATGC	4 cut(s)	379, 595, 1285, 2201
PflFI	GACNNNGTC	1 cut(s)	218
PkrI	GCNGC	9 cut(s)	448, 733, 793, 1105, 1339, 1426, 1504, 1915, 2184
Ple19I	CGATCG	1 cut(s)	381
PleI	GAGTC	5 cut(s)	133, 223, 605, 731, 1870
PpsI	GAGTC	5 cut(s)	133, 223, 605, 731, 1870
PpuMI	RGGWCCY	1 cut(s)	43
Psp1406I	AACGTT	1 cut(s)	954
Psp5II	RGGWCCY	1 cut(s)	43
Psp6I	CCWGG	1 cut(s)	1260
PspGI	CCWGG	1 cut(s)	1260
PspN4I	GGNNCC	3 cut(s)	44, 965, 1812
PspPI	GGNCC	3 cut(s)	43, 1086, 1824
PspPPI	RGGWCCY	1 cut(s)	43
PspXI	VCTCGAGB	2 cut(s)	613, 1373
PstI	CTGCAG	2 cut(s)	1756, 1999
PsuI	RGATCY	3 cut(s)	97, 963, 1991
PsyI	GACNNNGTC	1 cut(s)	218
PvuI	CGATCG	1 cut(s)	381
PvuII	CAGCTG	2 cut(s)	734, 767
RsaI	GTAC	3 cut(s)	676, 1977, 2148
RsaNI	GTAC	3 cut(s)	675, 1976, 2147
SatI	GCNGC	9 cut(s)	447, 732, 792, 1104, 1338, 1425, 1503, 1914, 2183
Sau96I	GGNCC	3 cut(s)	43, 1086, 1824
ScaI	AGTACT	1 cut(s)	676
SchI	GAGTC	5 cut(s)	134, 223, 605, 731, 1871
ScrFI	CCNGG	1 cut(s)	1262
SfaNI	GCATC	6 cut(s)	788, 880, 1036, 1199, 1349, 2114
SfcI	CTRYAG	3 cut(s)	1698, 1752, 1995
Sfr274I	CTCGAG	3 cut(s)	223, 613, 1373
SfuI	TTCGAA	2 cut(s)	1098, 1539
SinI	GGWCC	2 cut(s)	43, 1086
SlaI	CTCGAG	3 cut(s)	223, 613, 1373
SmlI	CTYRAG	4 cut(s)	223, 613, 911, 1373
SmoI	CTYRAG	4 cut(s)	223, 613, 911, 1373
SsiI	CCGC	9 cut(s)	118, 139, 447, 792, 816, 1084, 1104, 2109, 2183
SspI	AATATT	1 cut(s)	708
StyD4I	CCNGG	1 cut(s)	1260
TaaI	ACNGT	4 cut(s)	220, 281, 679, 1743
TaiI	ACGT	3 cut(s)	252, 436, 957
TatI	WGTACW	2 cut(s)	674, 1975
TauI	GCSGC	4 cut(s)	449, 794, 1106, 2185
TscAI	CASTG	3 cut(s)	310, 600, 1247
TseFI	GTSAC	1 cut(s)	826
TseI	GCWGC	5 cut(s)	731, 1337, 1424, 1502, 1913
Tsp45I	GTSAC	1 cut(s)	826
TspDTI	ATGAA	9 cut(s)	571, 916, 1119, 1172, 1367, 1464, 1512, 1658, 2146
TspGWI	ACGGA	4 cut(s)	72, 92, 338, 1975
TspRI	CASTG	3 cut(s)	310, 600, 1247
Tth111I	GACNNNGTC	1 cut(s)	218
Vha464I	CTTAAG	1 cut(s)	911
VpaK11BI	GGWCC	2 cut(s)	43, 1086
XagI	CCTNNNNNAGG	1 cut(s)	1315
XapI	RAATTY	7 cut(s)	387, 906, 1019, 1120, 1228, 1354, 1757
XbaI	TCTAGA	3 cut(s)	682, 1193, 1900
XceI	RCATGY	2 cut(s)	755, 1849
XhoI	CTCGAG	3 cut(s)	223, 613, 1373
ZraI	GACGTC	1 cut(s)	250
ZrmI	AGTACT	1 cut(s)	676
Zsp2I	ATGCAT	1 cut(s)	781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.