Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002975

Physical Location & Seq

Genomic Coordinates Reverse (-)

9271541 .. 9276648

Gene Structure 5108 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1107900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGGACGCCCTGTTCGACTCAATCAACGTCCGAGAGCTCCTGTCGGCGCAGGACCTCAGCGACCCCACGACCCCTCTGTCTGCGCCGGATCTCCGCCTCCTCATCCAACGCCTCGACTCCCACTCGCTCCAAATCAAGTCCAAAATCCAATCTTACCTCCTCTCTCACCACCAGGATTTCGCCAACCTTTTCTCCGTCTGCAACGACGCCGTTTCGCGCTCCAACCAAATTTCCGACGACGTCGTCCGGCTTCTGAGTTCAATTTCCAACCGCCCCATTGAGGCGGAGATTGGGCAGATTATGAAGCAGATGAGCGCCACGACGAAAGAGGCGAGGGAGAAGAAGGCGTTGTTGGAGTTGGTGAGGGCAATTCTGGAAATAAGTGAAAGGTTGAAGGGCGCCAGTGAGGGGTTGAGGAATGGACGGCTGAGGTTCGCCGCCCAGGAGCTGAGGGAGCTGAAGAATGCTCTTAGGGTTTCGGATGATGATCGGGTTGATGAGGGAGAGCCTGTGGTGTACAGATTGCTGAGGAAGGAGTGGTCAGAGTGCTTTGAGGAGATTCAAGAAGTGCTTGTGATGTTCATCAGAAATGCGGTGCGGTTTGAGCGGGAATCGAATAGAATCTGTGTCAAGTATGTACTTAGCATTGACGGAAACGATGGGTTTGAGCTCCGCACAGTTTTGGAAGCTTTGAATGTAGTCGGCATTTTGGATTATGGTCTTGGTAAAGTTGGCGACTTGATGATCAAGCATGTCATCTCCTCGGCTCTAAATTTTGGAGTTCCTGTGTCCTTTGTAGCTGAAGTAAATCGAGACTCACAAGTTCTGACTGAGGCGATACTGAACATTGTACCATCAAATGATCCTAAGATTGAAAAAATGGATGGTGAGACTATCTACTCAGGGATTATTCAGGTTATCAAGTTTATTAGCAACTACATCTGCCTTCAAGATGAGTCTTGGATTCGATGCTTTGGGAGATTGACATGGCCAAGGATTTCAGAGTTAATTATTTCTAAATTTCTCTCTAAGGTTGTTCCTGAAGATGCTTCAAAACTTGCTGACTTTCTTAAGATCATTAGTTGTACTTCCGAATTTGAGACTGCTTTAAGGGAAATGAAGTTTATTTCAGCATCTGATAACAGAGATAACCAGTTGAGCAATTTTGCTGAAAATGTCGAGGTTCACTTTGCATCCAGGAAGAAGTCAGAGATCTTGGCAAAAGCCAGAAATCTACTTCTACAATGTGATTTTTCTGCTCCTCAAGAGTATCCAAGGAATAACAAGAATGATCTGGATGCTGCCAAATCTCCAGAGCTTGTTAACTTGCTATTCCTGTCTGGGAAGTGTGTGGTGTCCAAAGCAGCGATTGAACTAATGAATCTAGTGCACCAGACACTTAAGGATGTTTGCATGTCATCCCCCAGAGTTGCTTTGGAGTTCTATCAGGCTGCTAGGGATGCTCTACTTCTCTATGAAGTCGTGGTTCCTGTCAAGCTAGAAAGGCATCTTGATGTAATCAACCAGGTTGCTGTTCTTATGCACAATGATTGCCTTTATCTATCTCAGGAGATACTGGGGCTTGCTTTTGAGTATCGCTCAGACTTTCCAAGTTCCATCAAAGAACATGCTATGTTTGTTGATATGGCTCCAAGATTCCATCTTATGGCAGAGGAAGTACTTCAGAGACAAATTCAACATGTTATTCGTAGTTTGAGAGAGGCTCTAGATGGTGCTGATGGATTTCAGAATACCCATGAAAGGCAGCAATTTCAATCTGCAAGTTGGAGTATAGATCAGGTTGTTTTGATTGTGGACAAAGTACGTTTGATATGGGAGCCGATCCTGTTGCCTTCAACTTACAAGAGAAGCATGTGTATGATTCTTGAATCGGTTTTTTCTAGGGTCATAAAAGACATACTCCTTTTAGATGATATGGCAGCAGAAGAAACATTACAGCTTCAAAGTCTGATTCATTTGATGCTGGAGAGCCTAATTTCTTTGCTGGAGTCCCTGGCTGCTCTCCAAGTAGAGAAGTCCCAAGAGGGCATCACTTGCACCCTTGACGATGACATACCTTCGTTATATAAAATTCGTAAATTGGCAGAATTATTAGATATGCCCTTAAAATCTATCACAACGGCTTGGGAAAGTGGAGAATTGCGCAATTGTGGTTTTACGTCGTCAGAGGTAGAGGATTTTATCAAGGCCATATTTGCAGATTCACCTTTGAGAAAAGAATGCTTAGCGAGGATCAAGGGCGAATGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000278 GO:0000775 GO:0000776 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006810 GO:0006888 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0016192 GO:0022402 GO:0030071 GO:0031577 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048193 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0098687 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:1990423 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Zw10

Protein Analysis

757

Amino Acids

85.78

Weight (kDa)

5.19

Isoelectric Point (pI)

45.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zw10_N	PF06248	38 - 131	5.9e-14	Centromere/kinetochore Zw10 N-terminal
Zw10_middle	PF20665	176 - 413	2.9e-50	Centromere/kinetochore protein zw10, middle domain
ZW10_C	PF20666	445 - 574	3.5e-41	Centromere/kinetochore protein zw10, C-terminal
ZW10_C2	PF22766	597 - 752	6.6e-39	ZW10 C-terminal helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32900
fragaria_vesca	FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900
malus_domestica	MD09G1116200.v1.1 MD17G1107900.v1.1
prunus_persica	Prupe.3G208500_v2.0.a1
pyrus_communis	pycom09g04060 pycom17g10050
rosa_chinensis	RchiOBHm_Chr0c20g0500271 RchiOBHm_Chr1g0340131 RchiOBHm_Chr1g0340141 RchiOBHm_Chr2g0157781
rosa_laevigata	RLG00000021018 RLG00000028993
rosa_multiflora	Rmu_co8468091.1_g000001 Rmu_sc0001275.1_g000033 Rmu_sc0010800.1_g000004 Rmu_sc0018661.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091960 Rroxscaffold_4G00311000
rosa_rugosa	Rorug02G0463300 Rorug02G0463300
rosa_samantha	Rh1AG180900 Rh1CG167700 Rh1DG181000 Rh1DG181100 Rh2AG528800 Rh2BG542700 Rh2CG513100 Rh2DG551700
rosa_wichuraiana	Rw1G015020 Rw2G043720

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	76, 242
AatII	GACGTC	1 cut(s)	243
Acc16I	TGCGCA	1 cut(s)	2165
AccB1I	GGYRCC	1 cut(s)	398
AccB7I	CCANNNNNTGG	1 cut(s)	1666
AccBSI	CCGCTC	1 cut(s)	607
AccII	CGCG	1 cut(s)	217
AciI	CCGC	8 cut(s)	94, 271, 284, 438, 593, 598, 607, 673
AclWI	GGATC	4 cut(s)	96, 857, 1837, 2261
AcoI	YGGCCR	1 cut(s)	989
AcsI	RAATTY	6 cut(s)	228, 772, 1019, 1094, 1692, 2091
AcuI	CTGAAG	4 cut(s)	479, 822, 1062, 1667
AcyI	GRCGYC	4 cut(s)	6, 207, 240, 399
AfaI	GTAC	6 cut(s)	518, 639, 852, 1087, 1680, 1824
AfiI	CCNNNNNNNGG	4 cut(s)	280, 408, 1666, 1761
AflII	CTTAAG	2 cut(s)	1070, 1400
AflIII	ACRYGT	1 cut(s)	1699
AjnI	CCWGG	5 cut(s)	171, 441, 1196, 1524, 2013
AjuI	GAANNNNNNNTTGG	2 cut(s)	335, 367
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	9 cut(s)	37, 448, 457, 670, 689, 800, 1318, 1498, 1960
AluI	AGCT	9 cut(s)	37, 448, 457, 670, 689, 800, 1318, 1498, 1960
Alw21I	GWGCWC	3 cut(s)	39, 672, 1392
Alw26I	GTCTC	4 cut(s)	807, 884, 1094, 1681
Alw44I	GTGCAC	1 cut(s)	1388
AlwI	GGATC	4 cut(s)	96, 857, 1837, 2261
AlwNI	CAGNNNCTG	2 cut(s)	448, 1136
AoxI	GGCC	2 cut(s)	989, 2208
ApaLI	GTGCAC	1 cut(s)	1388
ApeKI	GCWGC	6 cut(s)	1301, 1364, 1451, 1765, 1940, 2018
ApoI	RAATTY	6 cut(s)	228, 772, 1019, 1094, 1692, 2091
ArsI	GACNNNNNNTTYG	1 cut(s)	28
Asp700I	GAANNNNTTC	1 cut(s)	1680
AspLEI	GCGC	6 cut(s)	49, 85, 219, 317, 401, 2166
AspS9I	GGNCC	1 cut(s)	52
AsuHPI	GGTGA	4 cut(s)	158, 373, 899, 2217
AvaII	GGWCC	1 cut(s)	52
BaeGI	GKGCMC	1 cut(s)	1392
BalI	TGGCCA	1 cut(s)	991
BanI	GGYRCC	1 cut(s)	398
BanII	GRGCYC	2 cut(s)	39, 672
BarI	GAAGNNNNNNTAC	2 cut(s)	1938, 1970
Bbv12I	GWGCWC	3 cut(s)	39, 672, 1392
BbvCI	CCTCAGC	4 cut(s)	56, 428, 449, 527
BbvI	GCAGC	6 cut(s)	1288, 1376, 1438, 1777, 1952, 2005
BccI	CCATC	7 cut(s)	653, 862, 878, 1625, 1668, 1724, 1733
BceAI	ACGGC	3 cut(s)	194, 440, 2157
BcgI	CGANNNNNNTGC	2 cut(s)	1831, 1865
BciT130I	CCWGG	5 cut(s)	173, 443, 1198, 1526, 2015
BciVI	GTATCC	1 cut(s)	1281
BclI	TGATCA	1 cut(s)	744
BcoDI	GTCTC	4 cut(s)	807, 884, 1094, 1681
BfaI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
BfoI	RGCGCY	2 cut(s)	318, 402
BfrI	CTTAAG	2 cut(s)	1070, 1400
BfuI	GTATCC	1 cut(s)	1281
BglII	AGATCT	1 cut(s)	1212
BisI	GCNGC	7 cut(s)	438, 1302, 1365, 1452, 1766, 1941, 2019
BlpI	GCTNAGC	1 cut(s)	2245
BlsI	GCNGC	7 cut(s)	439, 1303, 1366, 1453, 1767, 1942, 2020
BmcAI	AGTACT	1 cut(s)	1680
Bme1390I	CCNGG	5 cut(s)	173, 443, 1198, 1526, 2015
Bme18I	GGWCC	1 cut(s)	52
BmgT120I	GGNCC	1 cut(s)	52
BmiI	GGNNCC	4 cut(s)	400, 1488, 1650, 1839
BmrFI	CCNGG	5 cut(s)	173, 443, 1198, 1526, 2015
BmrI	ACTGGG	1 cut(s)	1586
BmsI	GCATC	9 cut(s)	959, 1036, 1142, 1202, 1288, 1450, 1516, 1971, 2058
BmuI	ACTGGG	1 cut(s)	1586
BplI	GAGNNNNNCTC	4 cut(s)	1583, 1615, 1708, 1740
BpmI	CTGGAG	3 cut(s)	1296, 2006, 2027
Bpu10I	CCTNAGC	4 cut(s)	56, 428, 449, 527
Bpu1102I	GCTNAGC	1 cut(s)	2245
BpuEI	CTTGAG	1 cut(s)	1248
BsaBI	GATNNNNATC	2 cut(s)	486, 581
BsaHI	GRCGYC	4 cut(s)	6, 207, 240, 399
BsaJI	CCNNGG	5 cut(s)	441, 762, 992, 1274, 2013
BsaXI	ACNNNNNCTCC	6 cut(s)	176, 206, 495, 525, 771, 801
Bsc4I	CCNNNNNNNGG	4 cut(s)	280, 408, 1666, 1761
Bse1I	ACTGG	3 cut(s)	402, 1153, 1581
Bse8I	GATNNNNATC	2 cut(s)	486, 581
BseBI	CCWGG	5 cut(s)	173, 443, 1198, 1526, 2015
BseDI	CCNNGG	5 cut(s)	441, 762, 992, 1274, 2013
BseGI	GGATG	8 cut(s)	102, 487, 889, 1193, 1303, 1411, 1418, 1465
BseJI	GATNNNNATC	2 cut(s)	486, 581
BseLI	CCNNNNNNNGG	4 cut(s)	280, 408, 1666, 1761
BseMII	CTCAG	9 cut(s)	70, 245, 419, 440, 518, 822, 915, 1580, 1614
BseNI	ACTGG	3 cut(s)	402, 1153, 1581
BseRI	GAGGAG	5 cut(s)	89, 149, 569, 751, 1251
BseSI	GKGCMC	1 cut(s)	1392
BseXI	GCAGC	6 cut(s)	1288, 1376, 1438, 1777, 1952, 2005
Bsh1236I	CGCG	1 cut(s)	217
BshFI	GGCC	2 cut(s)	991, 2210
BshNI	GGYRCC	1 cut(s)	398
BsiHKAI	GWGCWC	3 cut(s)	39, 672, 1392
BsiSI	CCGG	2 cut(s)	86, 247
BslFI	GGGAC	2 cut(s)	1996, 2023
BslI	CCNNNNNNNGG	4 cut(s)	280, 408, 1666, 1761
BsmAI	GTCTC	4 cut(s)	807, 884, 1094, 1681
BsmFI	GGGAC	2 cut(s)	1996, 2023
BsmI	GAATGC	2 cut(s)	469, 2246
BsnI	GGCC	2 cut(s)	991, 2210
Bsp1286I	GDGCHC	3 cut(s)	39, 672, 1392
Bsp1407I	TGTACA	1 cut(s)	516
Bsp1720I	GCTNAGC	1 cut(s)	2245
BspACI	CCGC	8 cut(s)	94, 271, 284, 438, 593, 598, 607, 673
BspANI	GGCC	2 cut(s)	991, 2210
BspCNI	CTCAG	9 cut(s)	69, 246, 420, 441, 519, 823, 914, 1579, 1613
BspFNI	CGCG	1 cut(s)	217
BspLI	GGNNCC	4 cut(s)	400, 1488, 1650, 1839
BspPI	GGATC	4 cut(s)	96, 857, 1837, 2261
BspT107I	GGYRCC	1 cut(s)	398
BspTI	CTTAAG	2 cut(s)	1070, 1400
BsrBI	CCGCTC	1 cut(s)	607
BsrGI	TGTACA	1 cut(s)	516
BsrI	ACTGG	3 cut(s)	402, 1153, 1581
BssECI	CCNNGG	5 cut(s)	441, 762, 992, 1274, 2013
BssNI	GRCGYC	4 cut(s)	6, 207, 240, 399
BssT1I	CCWWGG	2 cut(s)	992, 1274
Bst2UI	CCWGG	5 cut(s)	173, 443, 1198, 1526, 2015
Bst4CI	ACNGT	1 cut(s)	679
BstACI	GRCGYC	4 cut(s)	6, 207, 240, 399
BstAFI	CTTAAG	2 cut(s)	1070, 1400
BstAPI	GCANNNNNTGC	1 cut(s)	2055
BstAUI	TGTACA	1 cut(s)	516
BstC8I	GCNNGC	1 cut(s)	1584
BstF5I	GGATG	8 cut(s)	102, 487, 889, 1193, 1303, 1411, 1418, 1465
BstFNI	CGCG	1 cut(s)	217
BstH2I	RGCGCY	2 cut(s)	318, 402
BstHHI	GCGC	6 cut(s)	49, 85, 219, 317, 401, 2166
BstMAI	GTCTC	4 cut(s)	807, 884, 1094, 1681
BstMWI	GCNNNNNNNGC	6 cut(s)	454, 604, 1460, 1504, 2055, 2216
BstNI	CCWGG	5 cut(s)	173, 443, 1198, 1526, 2015
BstNSI	RCATGY	5 cut(s)	755, 1417, 1631, 1703, 1876
BstSCI	CCNGG	5 cut(s)	171, 441, 1196, 1524, 2013
BstSLI	GKGCMC	1 cut(s)	1392
BstUI	CGCG	1 cut(s)	217
BstV1I	GCAGC	6 cut(s)	1288, 1376, 1438, 1777, 1952, 2005
BstX2I	RGATCY	2 cut(s)	88, 1212
BstYI	RGATCY	2 cut(s)	88, 1212
BsuI	GTATCC	1 cut(s)	1281
BsuRI	GGCC	2 cut(s)	991, 2210
BtsCI	GGATG	8 cut(s)	102, 487, 889, 1193, 1303, 1411, 1418, 1465
BtsIMutI	CAGTG	1 cut(s)	409
Cac8I	GCNNGC	1 cut(s)	1584
CaiI	CAGNNNCTG	2 cut(s)	448, 1136
CfoI	GCGC	6 cut(s)	49, 85, 219, 317, 401, 2166
Cfr13I	GGNCC	1 cut(s)	52
CseI	GACGC	2 cut(s)	14, 215
CsiI	ACCWGGT	1 cut(s)	1524
Csp6I	GTAC	6 cut(s)	517, 638, 851, 1086, 1679, 1823
CviAII	CATG	7 cut(s)	752, 987, 1414, 1628, 1700, 1757, 1873
CviQI	GTAC	6 cut(s)	517, 638, 851, 1086, 1679, 1823
DinI	GGCGCC	1 cut(s)	400
DrdI	GACNNNNNNGTC	2 cut(s)	76, 242
DseDI	GACNNNNNNGTC	2 cut(s)	76, 242
EaeI	YGGCCR	1 cut(s)	989
EciI	GGCGGA	2 cut(s)	83, 299
Ecl136II	GAGCTC	2 cut(s)	37, 670
Eco130I	CCWWGG	2 cut(s)	992, 1274
Eco24I	GRGCYC	2 cut(s)	39, 672
Eco47I	GGWCC	1 cut(s)	52
Eco53kI	GAGCTC	2 cut(s)	37, 670
Eco57I	CTGAAG	4 cut(s)	479, 822, 1062, 1667
EcoICRI	GAGCTC	2 cut(s)	37, 670
EcoO109I	RGGNCCY	1 cut(s)	52
EcoRII	CCWGG	5 cut(s)	171, 441, 1196, 1524, 2013
EcoT14I	CCWWGG	2 cut(s)	992, 1274
EcoT38I	GRGCYC	2 cut(s)	39, 672
EgeI	GGCGCC	1 cut(s)	400
EheI	GGCGCC	1 cut(s)	400
ErhI	CCWWGG	2 cut(s)	992, 1274
FaeI	CATG	7 cut(s)	755, 990, 1417, 1631, 1703, 1760, 1876
FalI	AAGNNNNNCTT	2 cut(s)	555, 587
FaqI	GGGAC	2 cut(s)	1996, 2023
FatI	CATG	7 cut(s)	751, 986, 1413, 1627, 1699, 1756, 1872
FauI	CCCGC	1 cut(s)	600
FbaI	TGATCA	1 cut(s)	744
Fnu4HI	GCNGC	7 cut(s)	438, 1302, 1365, 1452, 1766, 1941, 2019
FokI	GGATG	8 cut(s)	89, 494, 896, 1180, 1310, 1405, 1418, 1472
FriOI	GRGCYC	2 cut(s)	39, 672
Fsp4HI	GCNGC	7 cut(s)	438, 1302, 1365, 1452, 1766, 1941, 2019
FspBI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
FspI	TGCGCA	1 cut(s)	2165
GlaI	GCGC	6 cut(s)	48, 84, 218, 316, 400, 2165
GluI	GCNGC	7 cut(s)	438, 1302, 1365, 1452, 1766, 1941, 2019
GsuI	CTGGAG	3 cut(s)	1296, 2006, 2027
HaeII	RGCGCY	2 cut(s)	318, 402
HaeIII	GGCC	2 cut(s)	991, 2210
HapII	CCGG	2 cut(s)	86, 247
HgaI	GACGC	2 cut(s)	14, 215
HhaI	GCGC	6 cut(s)	49, 85, 219, 317, 401, 2166
Hin1I	GRCGYC	4 cut(s)	6, 207, 240, 399
Hin1II	CATG	7 cut(s)	755, 990, 1417, 1631, 1703, 1760, 1876
Hin6I	GCGC	6 cut(s)	47, 83, 217, 315, 399, 2164
HinP1I	GCGC	6 cut(s)	47, 83, 217, 315, 399, 2164
HincII	GTYRAC	1 cut(s)	1324
HindII	GTYRAC	1 cut(s)	1324
HindIII	AAGCTT	1 cut(s)	687
HpaI	GTTAAC	1 cut(s)	1324
HpaII	CCGG	2 cut(s)	86, 247
HphI	GGTGA	4 cut(s)	158, 373, 899, 2217
Hpy166II	GTNNAC	5 cut(s)	517, 1186, 1324, 1390, 1816
Hpy8I	GTNNAC	5 cut(s)	517, 1186, 1324, 1390, 1816
Hpy99I	CGWCG	6 cut(s)	209, 239, 242, 245, 325, 2185
HpyAV	CCTTC	6 cut(s)	337, 388, 526, 956, 1863, 2088
HpyCH4III	ACNGT	1 cut(s)	679
HpyCH4IV	ACGT	4 cut(s)	27, 240, 1825, 2180
HpyCH4V	TGCA	8 cut(s)	201, 1193, 1390, 1413, 1543, 1781, 2058, 2219
HpyF10VI	GCNNNNNNNGC	6 cut(s)	454, 604, 1460, 1504, 2055, 2216
HpySE526I	ACGT	4 cut(s)	27, 240, 1825, 2180
Hsp92I	GRCGYC	4 cut(s)	6, 207, 240, 399
Hsp92II	CATG	7 cut(s)	755, 990, 1417, 1631, 1703, 1760, 1876
HspAI	GCGC	6 cut(s)	47, 83, 217, 315, 399, 2164
KasI	GGCGCC	1 cut(s)	398
Ksp22I	TGATCA	1 cut(s)	744
KspAI	GTTAAC	1 cut(s)	1324
LmnI	GCTCC	9 cut(s)	42, 132, 224, 445, 454, 675, 1264, 1654, 1837
Lsp1109I	GCAGC	6 cut(s)	1288, 1376, 1438, 1777, 1952, 2005
LweI	GCATC	9 cut(s)	959, 1036, 1142, 1202, 1288, 1450, 1516, 1971, 2058
MabI	ACCWGGT	1 cut(s)	1524
MaeI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
MaeII	ACGT	4 cut(s)	27, 240, 1825, 2180
MbiI	CCGCTC	1 cut(s)	607
MboII	GAAGA	5 cut(s)	352, 472, 1055, 1213, 1958
MfeI	CAATTG	1 cut(s)	2167
MflI	RGATCY	2 cut(s)	88, 1212
MhlI	GDGCHC	3 cut(s)	39, 672, 1392
MlsI	TGGCCA	1 cut(s)	991
MluNI	TGGCCA	1 cut(s)	991
Mly113I	GGCGCC	1 cut(s)	399
MlyI	GAGTC	5 cut(s)	11, 110, 809, 965, 2018
MmeI	TCCRAC	6 cut(s)	130, 246, 258, 291, 333, 1766
Mox20I	TGGCCA	1 cut(s)	991
MroXI	GAANNNNTTC	1 cut(s)	1680
MscI	TGGCCA	1 cut(s)	991
MseI	TTAA	6 cut(s)	1007, 1071, 1109, 1323, 1401, 2126
MslI	CAYNNNNRTG	2 cut(s)	1512, 1877
Msp20I	TGGCCA	1 cut(s)	991
MspCI	CTTAAG	2 cut(s)	1070, 1400
MspI	CCGG	2 cut(s)	86, 247
MspR9I	CCNGG	5 cut(s)	173, 443, 1198, 1526, 2015
MunI	CAATTG	1 cut(s)	2167
Mva1269I	GAATGC	2 cut(s)	469, 2246
MvaI	CCWGG	5 cut(s)	173, 443, 1198, 1526, 2015
MvnI	CGCG	1 cut(s)	217
MwoI	GCNNNNNNNGC	6 cut(s)	454, 604, 1460, 1504, 2055, 2216
NarI	GGCGCC	1 cut(s)	399
NlaIII	CATG	7 cut(s)	755, 990, 1417, 1631, 1703, 1760, 1876
NlaIV	GGNNCC	4 cut(s)	400, 1488, 1650, 1839
NmeAIII	GCCGAG	1 cut(s)	743
NsbI	TGCGCA	1 cut(s)	2165
NspI	RCATGY	5 cut(s)	755, 1417, 1631, 1703, 1876
PciI	ACATGT	1 cut(s)	1699
PcsI	WCGNNNNNNNCGW	2 cut(s)	12, 329
PctI	GAATGC	2 cut(s)	469, 2246
PdmI	GAANNNNTTC	1 cut(s)	1680
PflFI	GACNNNGTC	2 cut(s)	239, 242
PflMI	CCANNNNNTGG	1 cut(s)	1666
PfoI	TCCNGGA	1 cut(s)	1196
PkrI	GCNGC	7 cut(s)	439, 1303, 1366, 1453, 1767, 1942, 2020
PleI	GAGTC	5 cut(s)	11, 110, 809, 964, 2017
PluTI	GGCGCC	1 cut(s)	402
PpsI	GAGTC	5 cut(s)	11, 110, 809, 964, 2017
PpuMI	RGGWCCY	1 cut(s)	52
PscI	ACATGT	1 cut(s)	1699
Psp124BI	GAGCTC	2 cut(s)	39, 672
Psp5II	RGGWCCY	1 cut(s)	52
Psp6I	CCWGG	5 cut(s)	171, 441, 1196, 1524, 2013
PspGI	CCWGG	5 cut(s)	171, 441, 1196, 1524, 2013
PspN4I	GGNNCC	4 cut(s)	400, 1488, 1650, 1839
PspPI	GGNCC	1 cut(s)	52
PspPPI	RGGWCCY	1 cut(s)	52
PstNI	CAGNNNCTG	2 cut(s)	448, 1136
PsuI	RGATCY	2 cut(s)	88, 1212
PsyI	GACNNNGTC	2 cut(s)	239, 242
RsaI	GTAC	6 cut(s)	518, 639, 852, 1087, 1680, 1824
RsaNI	GTAC	6 cut(s)	517, 638, 851, 1086, 1679, 1823
RseI	CAYNNNNRTG	2 cut(s)	1512, 1877
SacI	GAGCTC	2 cut(s)	39, 672
SaqAI	TTAA	6 cut(s)	1007, 1071, 1109, 1323, 1401, 2126
SatI	GCNGC	7 cut(s)	438, 1302, 1365, 1452, 1766, 1941, 2019
Sau96I	GGNCC	1 cut(s)	52
ScaI	AGTACT	1 cut(s)	1680
SchI	GAGTC	5 cut(s)	11, 110, 809, 965, 2018
ScrFI	CCNGG	5 cut(s)	173, 443, 1198, 1526, 2015
SduI	GDGCHC	3 cut(s)	39, 672, 1392
SexAI	ACCWGGT	1 cut(s)	1524
SfaNI	GCATC	9 cut(s)	959, 1036, 1142, 1202, 1288, 1450, 1516, 1971, 2058
SfoI	GGCGCC	1 cut(s)	400
SinI	GGWCC	1 cut(s)	52
SmiMI	CAYNNNNRTG	2 cut(s)	1512, 1877
SmlI	CTYRAG	3 cut(s)	1070, 1263, 1400
SmoI	CTYRAG	3 cut(s)	1070, 1263, 1400
SsiI	CCGC	8 cut(s)	94, 271, 284, 438, 593, 598, 607, 673
SspDI	GGCGCC	1 cut(s)	398
SspMI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
SstI	GAGCTC	2 cut(s)	39, 672
StyD4I	CCNGG	5 cut(s)	171, 441, 1196, 1524, 2013
StyI	CCWWGG	2 cut(s)	992, 1274
TaaI	ACNGT	1 cut(s)	679
TaiI	ACGT	4 cut(s)	30, 243, 1828, 2183
TaqI	TCGA	6 cut(s)	15, 114, 614, 811, 967, 1179
TatI	WGTACW	4 cut(s)	516, 637, 1085, 1678
TauI	GCSGC	1 cut(s)	440
Tru1I	TTAA	6 cut(s)	1007, 1071, 1109, 1323, 1401, 2126
Tru9I	TTAA	6 cut(s)	1007, 1071, 1109, 1323, 1401, 2126
TscAI	CASTG	1 cut(s)	409
TseI	GCWGC	6 cut(s)	1301, 1364, 1451, 1765, 1940, 2018
TspDTI	ATGAA	7 cut(s)	317, 571, 1133, 1394, 1491, 1773, 1964
TspGWI	ACGGA	2 cut(s)	184, 666
TspRI	CASTG	1 cut(s)	409
Tth111I	GACNNNGTC	2 cut(s)	239, 242
Van91I	CCANNNNNTGG	1 cut(s)	1666
Vha464I	CTTAAG	2 cut(s)	1070, 1400
VneI	GTGCAC	1 cut(s)	1388
VpaK11BI	GGWCC	1 cut(s)	52
XapI	RAATTY	6 cut(s)	228, 772, 1019, 1094, 1692, 2091
XbaI	TCTAGA	1 cut(s)	1726
XceI	RCATGY	5 cut(s)	755, 1417, 1631, 1703, 1876
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1432
XmnI	GAANNNNTTC	1 cut(s)	1680
XspI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
ZraI	GACGTC	1 cut(s)	241
ZrmI	AGTACT	1 cut(s)	1680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.