Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002975

Physical Location & Seq

Genomic Coordinates Forward (+)

70225046 .. 70229870

Gene Structure 4825 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G043720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGGAGGCTCTGTTCGACTCAATCAACGTCCGAGAGCTCCTCTCGGCGCAGGACCTCAGCGACCCCACCACACCTCTCTCCGCACCGGATCTCCGCCTCCTCATCCAACGCCTCGACTCCCACTCCCTCAAAATAAAGTCCAAAATCCAATCCTACCTCCTCTCTCACCACCACGACTTCGCCGACCTCTTCTCCCTCTGCAACGACGCCGTTTCCCGCTCCGTTAAAATCTCCGACGACGTCGTTCAGCTTCTCGCCTCACTCTCCGACCGCCCCGTCGAGGCCGAGATTGGCCAGATTATGAAGCAGATGAGCGCCACCACCAAAGAGGCGAGGGAGAAGCGGGAGCTGCTGGAGCTGGTGAGGGCAATTCTGGAAATAAGCGAAAAATTGAAGGCGGCTAGAGAGGCGTTGAGGAAGGGGCGGCTGAGATTTGCGGCTGAGCAATTGAGGGAGCTGAAGAAGGCTCTTAGGGTTTCCGATGATGATCGGGTTGATGAGAGTGAGCCTGTGGTTTATGGCTTGTTGAGGAAGGAGTGGTCAGACTGCTTTGATGAGATTCAAGAGGTGCTGATGAGGTTCATGGACAATGCGGTGCGTTTCGATCGCGAATCGAATAGAATTCAAGTCAAGTATGTACTCAGCATTGGTGGCAACGATGGGATTGAGCTCAAGACAGTGTTACAAGCTTTGGATGTGGTTGACATTTTACACTATGGACTTGCTAAAGTAGCTGACATGATAATCAAGCATTGCATCTCTCCACCTCTAAATTCTGGAGCACCTGTGTCCTTTGTAACAGAAATAAATCCAAACTCACAAGCAATGACTGAGGCGATACTGAAGATTGTACCATCAAATGATCCTAAGATTGAAAAAATGGACGGGGAGACTATCTATCCAAGGATTATTGAGGTTATAAAGTTTATTGACAAGTACATTTGCTTTCAAGAGGGATCTTGGACTCGATGCTTTGGAAGATTGACATGGCCAAGAATATCAGAGTTGATTATTTCTAAGTTTCTTTCTAAGGTTGTCCCCCAAGATGCTTCAAAACTTGCAGACTTTCTAAAGATCATCAATTGTACTTCTGAATTTGAGACTGCTTTAAGGGAGATGAAGTTTATTTCAGTATCTGAAAACAAAGATAACCAGTTGAGCAGTTTTGCTGAAAACGTCGAGGTTCACTTCGCATCCAGGAAGAAGACAGAAATCTTGGGAAGAGCTAGGAATTTGCTTCTACAATGTGATTTTGCTATTTCTCAAGAGAATACAAGGAGGAAAGGTAAGCACGATGGGGCTGCTAGCAATACACCAGAAGATGTTGACTTGCTATTTCTTTCGGAGCGGTGTGTGGTGTCCAAAGCAGCAATTCAATTAATGAAGTTAGTGCATGAGACACTCAAGGATGTCTGCTTGTCATCCCCTAGAGTTGCATTGGAATTTTATCGTGCAGCTAGGGATGTACTACTTCTATATGAAGTTGTCATACCTGTCAAGCTAGAACGGCAGCTTGATGGCATCAACCAGGTTGCTGTTCTGATGCACAACGATTGCCTTTTTCTATCTCAGGAGATTCTTGGGCTTGCATTTGAGTATCGCTCAGACTTTCCAACTTCAATGAAAGATCATGCTGTGTTTGTTGATATGGCTCCAAGATTTCATCTCATGGCAGAGGGAATACTGCAGAGACAAATCCAACTTGTGATTCATAGCTTGAGAGAGGCACTAGATGGCGCTGATGGATTCCAGAATACCCATCAAATGCAACAATTCCAATCTGCAAAATTTAGTATAGATCAGGTTGTTTTCATATTGGAGAAAGTACACATTATCTGGGAGCCACTCTTATTACCTTCAACTTACAAGAGAAGCATGTGTATGATTCTAGAGTCAGTTTTTTCTAGAATCACAAAAGACATACTCCTTTTAGATGATATGGCAGCAGAAGAAACGTTAGAGCTTCAAAGACTGATTCACTTGATGCTGGAAAACCTAACTTCTTTGTTGGGGTCCCTGGCTGCTGTTCAAGTTGAGAAGTCACAAGCGGGCATGACATCTCTTGATGATCTTATACCATCATTACGAAAAATTCGGAAATTGGCAGAATTATTAGATATGCCCTTGAAAGCAATCACTACAGCTTGGGAAACTGGAGAACTGCGAAATGGTGGCTTTACATTGTCAGAGGTAGAATATTTCATCAAAGCAATTTTTCAAGACTCGCCTTTGCGGAAAGACTGCTTATGGAGGATACATGGCTACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000278 GO:0000775 GO:0000776 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006810 GO:0006888 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0016192 GO:0022402 GO:0030071 GO:0031577 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048193 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0098687 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:1990423 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Zw10

Protein Analysis

756

Amino Acids

85.95

Weight (kDa)

5.72

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zw10_N	PF06248	38 - 131	1.6e-13	Centromere/kinetochore Zw10 N-terminal
Zw10_middle	PF20665	176 - 413	3.6e-50	Centromere/kinetochore protein zw10, middle domain
ZW10_C	PF20666	446 - 575	7.1e-40	Centromere/kinetochore protein zw10, C-terminal
ZW10_C2	PF22766	598 - 752	3.3e-40	ZW10 C-terminal helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32900
fragaria_vesca	FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900
malus_domestica	MD09G1116200.v1.1 MD17G1107900.v1.1
prunus_persica	Prupe.3G208500_v2.0.a1
pyrus_communis	pycom09g04060 pycom17g10050
rosa_chinensis	RchiOBHm_Chr0c20g0500271 RchiOBHm_Chr1g0340131 RchiOBHm_Chr1g0340141 RchiOBHm_Chr2g0157781
rosa_laevigata	RLG00000021018 RLG00000028993
rosa_multiflora	Rmu_co8468091.1_g000001 Rmu_sc0001275.1_g000033 Rmu_sc0010800.1_g000004 Rmu_sc0018661.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091960 Rroxscaffold_4G00311000
rosa_rugosa	Rorug02G0463300 Rorug02G0463300
rosa_samantha	Rh1AG180900 Rh1CG167700 Rh1DG181000 Rh1DG181100 Rh2AG528800 Rh2BG542700 Rh2CG513100 Rh2DG551700
rosa_wichuraiana	Rw1G015020 Rw2G043720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	920
AasI	GACNNNNNNGTC	1 cut(s)	275
AatII	GACGTC	1 cut(s)	243
AccBSI	CCGCTC	2 cut(s)	219, 1348
AccII	CGCG	1 cut(s)	609
AclI	AACGTT	1 cut(s)	1955
AclWI	GGATC	3 cut(s)	96, 857, 964
AcoI	YGGCCR	2 cut(s)	292, 989
AcsI	RAATTY	7 cut(s)	621, 772, 1094, 1231, 1442, 1787, 2091
AcuI	CTGAAG	2 cut(s)	479, 863
AcyI	GRCGYC	2 cut(s)	207, 240
AfaI	GTAC	6 cut(s)	639, 852, 938, 1087, 1467, 1827
AfiI	CCNNNNNNNGG	1 cut(s)	280
AjnI	CCWGG	3 cut(s)	1196, 1527, 2016
AjuI	GAANNNNNNNTTGG	2 cut(s)	1692, 1724
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	3 cut(s)	39, 672, 784
Alw26I	GTCTC	4 cut(s)	884, 1094, 1391, 1684
AlwI	GGATC	3 cut(s)	96, 857, 964
AlwNI	CAGNNNCTG	1 cut(s)	1136
AoxI	GGCC	3 cut(s)	282, 292, 989
ApeKI	GCWGC	7 cut(s)	349, 1301, 1367, 1454, 1510, 1943, 2021
ApoI	RAATTY	7 cut(s)	621, 772, 1094, 1231, 1442, 1787, 2091
AseI	ATTAAT	1 cut(s)	1379
AspLEI	GCGC	3 cut(s)	49, 317, 1739
AspS9I	GGNCC	2 cut(s)	52, 2013
AsuHPI	GGTGA	2 cut(s)	158, 373
AsuNHI	GCTAGC	1 cut(s)	1304
AvaII	GGWCC	2 cut(s)	52, 2013
BalI	TGGCCA	2 cut(s)	294, 991
BanII	GRGCYC	2 cut(s)	39, 672
BarI	GAAGNNNNNNTAC	2 cut(s)	1473, 1505
BbsI	GAAGAC	1 cut(s)	1211
Bbv12I	GWGCWC	3 cut(s)	39, 672, 784
BbvCI	CCTCAGC	1 cut(s)	56
BbvI	GCAGC	7 cut(s)	336, 1288, 1379, 1466, 1522, 1955, 2008
BccI	CCATC	8 cut(s)	653, 862, 1289, 1511, 1727, 1736, 1767, 2086
BceAI	ACGGC	2 cut(s)	194, 1523
BcgI	CGANNNNNNTGC	2 cut(s)	1283, 1317
BciT130I	CCWGG	3 cut(s)	1198, 1529, 2018
BciVI	GTATCC	1 cut(s)	2247
BcoDI	GTCTC	4 cut(s)	884, 1094, 1391, 1684
BfaI	CTAG	9 cut(s)	402, 1227, 1305, 1428, 1458, 1502, 1730, 1889, 1905
BfmI	CTRYAG	2 cut(s)	1685, 2139
BfoI	RGCGCY	2 cut(s)	318, 1740
BfuI	GTATCC	1 cut(s)	2247
BlpI	GCTNAGC	1 cut(s)	441
Bme1390I	CCNGG	3 cut(s)	1198, 1529, 2018
Bme18I	GGWCC	2 cut(s)	52, 2013
BmgT120I	GGNCC	2 cut(s)	52, 2013
BmiI	GGNNCC	4 cut(s)	1653, 1842, 2014, 2015
BmrFI	CCNGG	3 cut(s)	1198, 1529, 2018
BmsI	GCATC	7 cut(s)	765, 959, 1036, 1202, 1530, 1533, 1974
BmtI	GCTAGC	1 cut(s)	1308
BpiI	GAAGAC	1 cut(s)	1211
BplI	GAGNNNNNCTC	6 cut(s)	24, 56, 26, 58, 1586, 1618
BpmI	CTGGAG	3 cut(s)	374, 798, 2175
Bpu10I	CCTNAGC	1 cut(s)	56
Bpu1102I	GCTNAGC	1 cut(s)	441
BpuEI	CTTGAG	4 cut(s)	656, 1248, 1388, 1738
BsaBI	GATNNNNATC	1 cut(s)	486
BsaHI	GRCGYC	2 cut(s)	207, 240
BsaJI	CCNNGG	2 cut(s)	902, 2016
BsaWI	WCCGGW	1 cut(s)	85
Bsc4I	CCNNNNNNNGG	1 cut(s)	280
Bse1I	ACTGG	2 cut(s)	1153, 2158
Bse3DI	GCAATG	2 cut(s)	751, 831
Bse8I	GATNNNNATC	1 cut(s)	486
BseBI	CCWGG	3 cut(s)	1198, 1529, 2018
BseDI	CCNNGG	2 cut(s)	902, 2016
BseGI	GGATG	6 cut(s)	102, 700, 1193, 1414, 1421, 1468
BseJI	GATNNNNATC	1 cut(s)	486
BseLI	CCNNNNNNNGG	1 cut(s)	280
BseMI	GCAATG	2 cut(s)	751, 831
BseMII	CTCAG	7 cut(s)	70, 419, 432, 655, 822, 1583, 1617
BseNI	ACTGG	2 cut(s)	1153, 2158
BseRI	GAGGAG	3 cut(s)	29, 89, 149
BseXI	GCAGC	7 cut(s)	336, 1288, 1379, 1466, 1522, 1955, 2008
BsgI	GTGCAG	1 cut(s)	1473
Bsh1236I	CGCG	1 cut(s)	609
Bsh1285I	CGRYCG	2 cut(s)	271, 607
BshFI	GGCC	3 cut(s)	284, 294, 991
BsiEI	CGRYCG	2 cut(s)	271, 607
BsiHKAI	GWGCWC	3 cut(s)	39, 672, 784
BsiSI	CCGG	1 cut(s)	86
BslFI	GGGAC	2 cut(s)	1022, 1999
BslI	CCNNNNNNNGG	1 cut(s)	280
BsmAI	GTCTC	4 cut(s)	884, 1094, 1391, 1684
BsmFI	GGGAC	2 cut(s)	1022, 1999
BsnI	GGCC	3 cut(s)	284, 294, 991
Bsp1286I	GDGCHC	3 cut(s)	39, 672, 784
Bsp143I	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
Bsp1720I	GCTNAGC	1 cut(s)	441
Bsp68I	TCGCGA	1 cut(s)	609
BspANI	GGCC	3 cut(s)	284, 294, 991
BspCNI	CTCAG	7 cut(s)	69, 420, 433, 654, 823, 1582, 1616
BspFNI	CGCG	1 cut(s)	609
BspLI	GGNNCC	4 cut(s)	1653, 1842, 2014, 2015
BspMAI	CTGCAG	1 cut(s)	1689
BspOI	GCTAGC	1 cut(s)	1308
BspPI	GGATC	3 cut(s)	96, 857, 964
BspQI	GCTCTTC	1 cut(s)	1216
BsrBI	CCGCTC	2 cut(s)	219, 1348
BsrDI	GCAATG	2 cut(s)	751, 831
BsrI	ACTGG	2 cut(s)	1153, 2158
BssECI	CCNNGG	2 cut(s)	902, 2016
BssMI	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
BssNI	GRCGYC	2 cut(s)	207, 240
BssT1I	CCWWGG	1 cut(s)	902
Bst2UI	CCWGG	3 cut(s)	1198, 1529, 2018
Bst4CI	ACNGT	1 cut(s)	679
Bst6I	CTCTTC	2 cut(s)	194, 1216
BstACI	GRCGYC	2 cut(s)	207, 240
BstC8I	GCNNGC	3 cut(s)	1306, 1587, 2050
BstF5I	GGATG	6 cut(s)	102, 700, 1193, 1414, 1421, 1468
BstFNI	CGCG	1 cut(s)	609
BstH2I	RGCGCY	2 cut(s)	318, 1740
BstHHI	GCGC	3 cut(s)	49, 317, 1739
BstKTI	GATC	9 cut(s)	91, 490, 607, 865, 959, 1077, 1630, 1801, 2071
BstMAI	GTCTC	4 cut(s)	884, 1094, 1391, 1684
BstMBI	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
BstMCI	CGRYCG	2 cut(s)	271, 607
BstMWI	GCNNNNNNNGC	6 cut(s)	349, 355, 407, 651, 731, 1507
BstNI	CCWGG	3 cut(s)	1198, 1529, 2018
BstNSI	RCATGY	1 cut(s)	1879
BstSCI	CCNGG	3 cut(s)	1196, 1527, 2016
BstSFI	CTRYAG	2 cut(s)	1685, 2139
BstUI	CGCG	1 cut(s)	609
BstV1I	GCAGC	7 cut(s)	336, 1288, 1379, 1466, 1522, 1955, 2008
BstV2I	GAAGAC	1 cut(s)	1211
BstX2I	RGATCY	2 cut(s)	88, 956
BstYI	RGATCY	2 cut(s)	88, 956
BsuI	GTATCC	1 cut(s)	2247
BsuRI	GGCC	3 cut(s)	284, 294, 991
BtsCI	GGATG	6 cut(s)	102, 700, 1193, 1414, 1421, 1468
BtsIMutI	CAGTG	1 cut(s)	684
BtuMI	TCGCGA	1 cut(s)	609
Cac8I	GCNNGC	3 cut(s)	1306, 1587, 2050
CaiI	CAGNNNCTG	1 cut(s)	1136
CfoI	GCGC	3 cut(s)	49, 317, 1739
Cfr13I	GGNCC	2 cut(s)	52, 2013
CseI	GACGC	1 cut(s)	215
CsiI	ACCWGGT	1 cut(s)	1527
Csp6I	GTAC	6 cut(s)	638, 851, 937, 1086, 1466, 1826
CviAII	CATG	9 cut(s)	583, 739, 987, 1394, 1631, 1669, 1876, 2053, 2258
CviQI	GTAC	6 cut(s)	638, 851, 937, 1086, 1466, 1826
DpnI	GATC	9 cut(s)	90, 489, 606, 864, 958, 1076, 1629, 1800, 2070
DpnII	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
DrdI	GACNNNNNNGTC	1 cut(s)	275
DseDI	GACNNNNNNGTC	1 cut(s)	275
EaeI	YGGCCR	2 cut(s)	292, 989
Eam1104I	CTCTTC	2 cut(s)	194, 1216
EarI	CTCTTC	2 cut(s)	194, 1216
EciI	GGCGGA	1 cut(s)	83
Ecl136II	GAGCTC	2 cut(s)	37, 670
Eco130I	CCWWGG	1 cut(s)	902
Eco24I	GRGCYC	2 cut(s)	39, 672
Eco47I	GGWCC	2 cut(s)	52, 2013
Eco53kI	GAGCTC	2 cut(s)	37, 670
Eco57I	CTGAAG	2 cut(s)	479, 863
EcoICRI	GAGCTC	2 cut(s)	37, 670
EcoO109I	RGGNCCY	2 cut(s)	52, 2013
EcoRI	GAATTC	1 cut(s)	621
EcoRII	CCWGG	3 cut(s)	1196, 1527, 2016
EcoT14I	CCWWGG	1 cut(s)	902
EcoT38I	GRGCYC	2 cut(s)	39, 672
ErhI	CCWWGG	1 cut(s)	902
FaeI	CATG	9 cut(s)	586, 742, 990, 1397, 1634, 1672, 1879, 2056, 2261
FaqI	GGGAC	2 cut(s)	1022, 1999
FatI	CATG	9 cut(s)	582, 738, 986, 1393, 1630, 1668, 1875, 2052, 2257
FauI	CCCGC	3 cut(s)	224, 336, 2041
FokI	GGATG	6 cut(s)	89, 707, 1180, 1408, 1421, 1475
FriOI	GRGCYC	2 cut(s)	39, 672
FspBI	CTAG	9 cut(s)	402, 1227, 1305, 1428, 1458, 1502, 1730, 1889, 1905
GlaI	GCGC	3 cut(s)	48, 316, 1738
GsuI	CTGGAG	3 cut(s)	374, 798, 2175
HaeII	RGCGCY	2 cut(s)	318, 1740
HaeIII	GGCC	3 cut(s)	284, 294, 991
HapII	CCGG	1 cut(s)	86
HgaI	GACGC	1 cut(s)	215
HhaI	GCGC	3 cut(s)	49, 317, 1739
Hin1I	GRCGYC	2 cut(s)	207, 240
Hin1II	CATG	9 cut(s)	586, 742, 990, 1397, 1634, 1672, 1879, 2056, 2261
Hin6I	GCGC	3 cut(s)	47, 315, 1737
HinP1I	GCGC	3 cut(s)	47, 315, 1737
HincII	GTYRAC	2 cut(s)	703, 1327
HindII	GTYRAC	2 cut(s)	703, 1327
HindIII	AAGCTT	1 cut(s)	687
HpaII	CCGG	1 cut(s)	86
HphI	GGTGA	2 cut(s)	158, 373
Hpy166II	GTNNAC	4 cut(s)	703, 1186, 1327, 1828
Hpy8I	GTNNAC	4 cut(s)	703, 1186, 1327, 1828
Hpy99I	CGWCG	6 cut(s)	209, 239, 242, 245, 281, 1181
HpyAV	CCTTC	5 cut(s)	388, 412, 457, 526, 1866
HpyCH4III	ACNGT	1 cut(s)	679
HpyCH4IV	ACGT	4 cut(s)	27, 240, 1176, 1955
HpyF10VI	GCNNNNNNNGC	6 cut(s)	349, 355, 407, 651, 731, 1507
HpySE526I	ACGT	4 cut(s)	27, 240, 1176, 1955
Hsp92I	GRCGYC	2 cut(s)	207, 240
Hsp92II	CATG	9 cut(s)	586, 742, 990, 1397, 1634, 1672, 1879, 2056, 2261
HspAI	GCGC	3 cut(s)	47, 315, 1737
KflI	GGGWCCC	1 cut(s)	2013
Kzo9I	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
LguI	GCTCTTC	1 cut(s)	1216
LmnI	GCTCC	9 cut(s)	42, 224, 346, 355, 454, 779, 1345, 1657, 1840
Lsp1109I	GCAGC	7 cut(s)	336, 1288, 1379, 1466, 1522, 1955, 2008
LweI	GCATC	7 cut(s)	765, 959, 1036, 1202, 1530, 1533, 1974
MabI	ACCWGGT	1 cut(s)	1527
MaeI	CTAG	9 cut(s)	402, 1227, 1305, 1428, 1458, 1502, 1730, 1889, 1905
MaeII	ACGT	4 cut(s)	27, 240, 1176, 1955
MaeIII	GTNAC	3 cut(s)	681, 796, 2040
MalI	GATC	9 cut(s)	90, 489, 606, 864, 958, 1076, 1629, 1800, 2070
MbiI	CCGCTC	2 cut(s)	219, 1348
MboI	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
MboII	GAAGA	9 cut(s)	181, 472, 856, 990, 1213, 1216, 1233, 1331, 1961
MfeI	CAATTG	2 cut(s)	446, 1081
MflI	RGATCY	2 cut(s)	88, 956
MhlI	GDGCHC	3 cut(s)	39, 672, 784
MlsI	TGGCCA	2 cut(s)	294, 991
MluNI	TGGCCA	2 cut(s)	294, 991
MlyI	GAGTC	5 cut(s)	11, 110, 958, 1901, 2216
MmeI	TCCRAC	5 cut(s)	130, 258, 291, 1637, 1723
Mox20I	TGGCCA	2 cut(s)	294, 991
MscI	TGGCCA	2 cut(s)	294, 991
MseI	TTAA	4 cut(s)	225, 1109, 1379, 2269
MslI	CAYNNNNRTG	1 cut(s)	1880
Msp20I	TGGCCA	2 cut(s)	294, 991
MspI	CCGG	1 cut(s)	86
MspR9I	CCNGG	3 cut(s)	1198, 1529, 2018
MunI	CAATTG	2 cut(s)	446, 1081
MvaI	CCWGG	3 cut(s)	1198, 1529, 2018
MvnI	CGCG	1 cut(s)	609
MwoI	GCNNNNNNNGC	6 cut(s)	349, 355, 407, 651, 731, 1507
NdeII	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
NheI	GCTAGC	1 cut(s)	1304
NlaIII	CATG	9 cut(s)	586, 742, 990, 1397, 1634, 1672, 1879, 2056, 2261
NlaIV	GGNNCC	4 cut(s)	1653, 1842, 2014, 2015
NmeAIII	GCCGAG	2 cut(s)	23, 310
NmuCI	GTSAC	1 cut(s)	2040
NruI	TCGCGA	1 cut(s)	609
NspI	RCATGY	1 cut(s)	1879
PciSI	GCTCTTC	1 cut(s)	1216
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PfeI	GAWTC	8 cut(s)	559, 611, 1576, 1708, 1746, 1885, 1908, 1975
PflFI	GACNNNGTC	1 cut(s)	239
PfoI	TCCNGGA	1 cut(s)	1196
Ple19I	CGATCG	1 cut(s)	607
PleI	GAGTC	5 cut(s)	11, 110, 958, 1900, 2216
PpsI	GAGTC	5 cut(s)	11, 110, 958, 1900, 2216
PpuMI	RGGWCCY	2 cut(s)	52, 2013
PshBI	ATTAAT	1 cut(s)	1379
PsiI	TTATAA	1 cut(s)	920
Psp124BI	GAGCTC	2 cut(s)	39, 672
Psp1406I	AACGTT	1 cut(s)	1955
Psp5II	RGGWCCY	2 cut(s)	52, 2013
Psp6I	CCWGG	3 cut(s)	1196, 1527, 2016
PspGI	CCWGG	3 cut(s)	1196, 1527, 2016
PspN4I	GGNNCC	4 cut(s)	1653, 1842, 2014, 2015
PspPI	GGNCC	2 cut(s)	52, 2013
PspPPI	RGGWCCY	2 cut(s)	52, 2013
PstI	CTGCAG	1 cut(s)	1689
PstNI	CAGNNNCTG	1 cut(s)	1136
PsuI	RGATCY	2 cut(s)	88, 956
PsyI	GACNNNGTC	1 cut(s)	239
PvuI	CGATCG	1 cut(s)	607
RruI	TCGCGA	1 cut(s)	609
RsaI	GTAC	6 cut(s)	639, 852, 938, 1087, 1467, 1827
RsaNI	GTAC	6 cut(s)	638, 851, 937, 1086, 1466, 1826
RseI	CAYNNNNRTG	1 cut(s)	1880
SacI	GAGCTC	2 cut(s)	39, 672
SapI	GCTCTTC	1 cut(s)	1216
SaqAI	TTAA	4 cut(s)	225, 1109, 1379, 2269
Sau3AI	GATC	9 cut(s)	88, 487, 604, 862, 956, 1074, 1627, 1798, 2068
Sau96I	GGNCC	2 cut(s)	52, 2013
SchI	GAGTC	5 cut(s)	11, 110, 958, 1901, 2216
ScrFI	CCNGG	3 cut(s)	1198, 1529, 2018
SduI	GDGCHC	3 cut(s)	39, 672, 784
SexAI	ACCWGGT	1 cut(s)	1527
SfaNI	GCATC	7 cut(s)	765, 959, 1036, 1202, 1530, 1533, 1974
SfcI	CTRYAG	2 cut(s)	1685, 2139
SinI	GGWCC	2 cut(s)	52, 2013
SmiMI	CAYNNNNRTG	1 cut(s)	1880
SmlI	CTYRAG	4 cut(s)	671, 1263, 1403, 1717
SmoI	CTYRAG	4 cut(s)	671, 1263, 1403, 1717
SspI	AATATT	1 cut(s)	2198
SspMI	CTAG	9 cut(s)	402, 1227, 1305, 1428, 1458, 1502, 1730, 1889, 1905
SstI	GAGCTC	2 cut(s)	39, 672
StyD4I	CCNGG	3 cut(s)	1196, 1527, 2016
StyI	CCWWGG	1 cut(s)	902
TaaI	ACNGT	1 cut(s)	679
TaiI	ACGT	4 cut(s)	30, 243, 1179, 1958
TaqI	TCGA	7 cut(s)	15, 114, 279, 603, 614, 967, 1179
TatI	WGTACW	5 cut(s)	637, 936, 1085, 1465, 1825
TauI	GCSGC	3 cut(s)	401, 427, 440
TfiI	GAWTC	8 cut(s)	559, 611, 1576, 1708, 1746, 1885, 1908, 1975
Tru1I	TTAA	4 cut(s)	225, 1109, 1379, 2269
Tru9I	TTAA	4 cut(s)	225, 1109, 1379, 2269
TscAI	CASTG	1 cut(s)	684
TseFI	GTSAC	1 cut(s)	2040
TseI	GCWGC	7 cut(s)	349, 1301, 1367, 1454, 1510, 1943, 2021
Tsp45I	GTSAC	1 cut(s)	2040
TspGWI	ACGGA	1 cut(s)	211
TspRI	CASTG	1 cut(s)	684
Tth111I	GACNNNGTC	1 cut(s)	239
VpaK11BI	GGWCC	2 cut(s)	52, 2013
VspI	ATTAAT	1 cut(s)	1379
XapI	RAATTY	7 cut(s)	621, 772, 1094, 1231, 1442, 1787, 2091
XbaI	TCTAGA	2 cut(s)	1888, 1904
XceI	RCATGY	1 cut(s)	1879
XspI	CTAG	9 cut(s)	402, 1227, 1305, 1428, 1458, 1502, 1730, 1889, 1905
ZraI	GACGTC	1 cut(s)	241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.