Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002975

Physical Location & Seq

Genomic Coordinates Reverse (-)

8928127 .. 8933194

Gene Structure 5068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1116200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGGACGCCCTGTTCGACTCAATCAACGTTCGAGAGCTCCTGTCGGCGCAGGACCTCAGCGACCCCACGACCCCTCTGTCCGCACCGGATCTCCGCCTCCTCATCCAACGCCTCGACTCCCACTCGCTCCAAATCAAGTCCAAAATCCAATCTTACCTCCTCTCTCACCACCAGGATTTCGCCAACCTCTTCTCCGTCTGCAACGACGCCGTTACGCGCTCCAATCAAATCTCCGACGACGTCGTTCAGCTTCTGAGTTCGATTTCCGACCGCCCGATCGAGGCGGAGATCGGACAGATTATAAAGAAGATGAGCGCTACGACGAAAGAGGCGAGGGAGAAGAAGGCATTGTTGGAGTTGGTGAGGGCAATTCTGGCAATAAGTGAAAAGTTGAATGGCGCGAGAGAGGGGTTGAGGAATGGACGGCTGAGATTCGCCGCCAAGGAACTGAGGGAGCTGAAGAAAGCTCTTAGGATTTCTGATGATGATCGTGTCGATGAGAGAGAGCCAGTGGTGTACAGCTTGCTGAGGAAGGAGTGGTCAGAATGCTTTCAGGAGATTCAAGAGGTGCTTGTGAGGTTCATCGGAAATGCGGTGCGGTTTGAGCGGGAATTGAATAGAATCCGTGTCAAGTATGTACTAAACATTGATGGAAACGATGGGATTGAGCTCCGCACAGTTTTGGAAGCTTTGGATGTGGTTGGAATTTTGGATTATGGTCTTGCTAAAGTAGCTGACTTGATGATCAAGCATGTCATCTCACCGGGTCTAAATTTTGGAGCTCCTGTCTCCTTTGTAGCTGAAGTAAATCAAGACCCTAAAGTACTGACTGAGGCAATACTGAACATTGTGCCATCAAATGATCCAAAGATTGAAAAAATGGATGGCAAGACTATCTATTCAGGGATTATTCAGGTTATCAAGTTTATTAGCAACTATATCTGCCTTCAAGATGAATCTTGGATTCGATGCTTTGGGAGATTGACATGGCCAAGGATTTCAGAGTTAATTATTTCTAAATTTCTTTCGAAGGTTGTTCCTGAAGATGCTTCAAAACTTGCTGACTTTTTGAAGATCATTAGTTGTACTTCCGAATTTGAGACTGCTTTAAGGGAAATGAAGTTTATTTCAGCATCTGATAACAAAGATAACCAGTTGAGCAATTTTGCTGAAAATGTCGAGGTTCACTTTGCATCCAGGAAGAAGACAGAGATCCTGGCAAAAGCCAGAAATCTGCTTCTACAATGTGATTTTTCTACTCTTCATGAGTATCCAAGGAATAACAAGAATGATCGGGATGCTGCCAAATCTCCAGAGCTTGTTGACTTGCTATTCCTGTCGGGGAGGTGTGTGGTGTCCAAAGCAGCAATTGAACTAATGAAGCTAGTGCACCAGACACTTCAGGATGTTTGCGTGTCATCCCCCAGAGTTGCTTTGGAGTTTTATCGGGCTGCTAGGGATGCTCTACTTCTATATGAAGTTGTGGTTCCTGTCAAGCTAGAAAGGCAGCTTGATGGAATCAACCATGTTGCTGTTCTTATGCACAATGATTGCTTTTATCTATCTCAGGAGATACTTGGGCTTGCTTTCGAGTATCGCTCAGACTTTCCAAGTTCCATCAAAGAACATGCTATGTTTGTTGATATGGCTCCAAGATTCCATATTATAGCAGAGGAAATACTGCAGAGACAAATTCAACATGTTATTCGTAGTTTGAGAGAGGCTCTAGATGGCGCTGATGGATTTCAGAATACCCATGAAAGGCAACAATTTCAATCTGCAAATTTGAGTATAGATCAGGTTGTTTTTATTCTGGACAAAGTACGTCTGATATGGGAGCCAATCTTGTTGCCTTCAACTTACAAGAGAAGCATTTGTGTTGTGCTGGAATCGGTTTTTTCTAGAGTGACAAAAGACATACTCCTTTTAGATGATATGGCAGCAGAAGAAACATTACAGCTTCAAAGTCTGATTCATTTGATGCTGGAAAGCCTAATTTCTTTGCTGGAGTCCCTTGCTGCCATCCAAGTAGAGAAGTCACAAGAGGGCATTGCTTGCACCATTGATGATTTCATACCTTCGTTATATAAAATTCGTAAATTGGCAGAATTATTAGATATGCCCTTAAAATCCATTACAGCAGCTTGGGAAAGTGGAGAGTTGCGCAGTTGTGGCTTTACGTCGTCAGAGGTAGAAGATTTTATCAAGGCGATATTTGCAGATTCACCTTTGAGAAAAGAATGCTTGGCGAGGATAAAGGGCGACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000278 GO:0000775 GO:0000776 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006810 GO:0006888 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0016192 GO:0022402 GO:0030071 GO:0031577 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048193 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0098687 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:1990423 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Zw10

Protein Analysis

757

Amino Acids

85.72

Weight (kDa)

5.5

Isoelectric Point (pI)

46.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zw10_N	PF06248	38 - 131	5e-13	Centromere/kinetochore Zw10 N-terminal
Zw10_middle	PF20665	176 - 413	3.9e-50	Centromere/kinetochore protein zw10, middle domain
ZW10_C	PF20666	445 - 574	7e-41	Centromere/kinetochore protein zw10, C-terminal
ZW10_C2	PF22766	597 - 752	4.1e-39	ZW10 C-terminal helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32900
fragaria_vesca	FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900 FvH4_6g41900
malus_domestica	MD09G1116200.v1.1 MD17G1107900.v1.1
prunus_persica	Prupe.3G208500_v2.0.a1
pyrus_communis	pycom09g04060 pycom17g10050
rosa_chinensis	RchiOBHm_Chr0c20g0500271 RchiOBHm_Chr1g0340131 RchiOBHm_Chr1g0340141 RchiOBHm_Chr2g0157781
rosa_laevigata	RLG00000021018 RLG00000028993
rosa_multiflora	Rmu_co8468091.1_g000001 Rmu_sc0001275.1_g000033 Rmu_sc0010800.1_g000004 Rmu_sc0018661.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091960 Rroxscaffold_4G00311000
rosa_rugosa	Rorug02G0463300 Rorug02G0463300
rosa_samantha	Rh1AG180900 Rh1CG167700 Rh1DG181000 Rh1DG181100 Rh2AG528800 Rh2BG542700 Rh2CG513100 Rh2DG551700
rosa_wichuraiana	Rw1G015020 Rw2G043720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	302
AasI	GACNNNNNNGTC	1 cut(s)	76
AatII	GACGTC	1 cut(s)	243
Acc16I	TGCGCA	1 cut(s)	2165
AccBSI	CCGCTC	1 cut(s)	607
AccII	CGCG	2 cut(s)	217, 401
AciI	CCGC	9 cut(s)	81, 94, 271, 284, 438, 593, 598, 607, 673
AclI	AACGTT	1 cut(s)	27
AclWI	GGATC	3 cut(s)	96, 857, 1207
AcoI	YGGCCR	1 cut(s)	989
AcsI	RAATTY	7 cut(s)	705, 772, 1019, 1094, 1692, 1783, 2091
AcuI	CTGAAG	4 cut(s)	479, 822, 1062, 1385
AcyI	GRCGYC	3 cut(s)	6, 207, 240
AfaI	GTAC	5 cut(s)	518, 639, 825, 1087, 1824
AfeI	AGCGCT	1 cut(s)	316
AfiI	CCNNNNNNNGG	2 cut(s)	280, 1761
AflIII	ACRYGT	1 cut(s)	1699
AjnI	CCWGG	3 cut(s)	171, 1196, 1215
AjuI	GAANNNNNNNTTGG	2 cut(s)	335, 367
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	4 cut(s)	39, 672, 784, 1392
Alw26I	GTCTC	3 cut(s)	793, 1094, 1681
Alw44I	GTGCAC	1 cut(s)	1388
AlwI	GGATC	3 cut(s)	96, 857, 1207
AlwNI	CAGNNNCTG	2 cut(s)	253, 1136
Aor51HI	AGCGCT	1 cut(s)	316
AoxI	GGCC	1 cut(s)	989
ApaLI	GTGCAC	1 cut(s)	1388
ApeKI	GCWGC	7 cut(s)	1301, 1364, 1451, 1507, 1940, 2018, 2141
ApoI	RAATTY	7 cut(s)	705, 772, 1019, 1094, 1692, 1783, 2091
ArsI	GACNNNNNNTTYG	1 cut(s)	28
Asp700I	GAANNNNTTC	1 cut(s)	549
AspLEI	GCGC	6 cut(s)	49, 219, 317, 401, 1736, 2166
AspS9I	GGNCC	1 cut(s)	52
AsuC2I	CCSGG	1 cut(s)	765
AsuHPI	GGTGA	4 cut(s)	158, 373, 753, 2217
AsuII	TTCGAA	1 cut(s)	1028
AvaII	GGWCC	1 cut(s)	52
BaeGI	GKGCMC	1 cut(s)	1392
BalI	TGGCCA	1 cut(s)	991
BanII	GRGCYC	3 cut(s)	39, 672, 784
BarI	GAAGNNNNNNTAC	2 cut(s)	1938, 1970
BbsI	GAAGAC	1 cut(s)	1211
Bbv12I	GWGCWC	4 cut(s)	39, 672, 784, 1392
BbvCI	CCTCAGC	2 cut(s)	56, 527
BbvI	GCAGC	7 cut(s)	1288, 1376, 1438, 1519, 1952, 2005, 2153
BccI	CCATC	9 cut(s)	644, 653, 862, 878, 1508, 1625, 1724, 1733, 2030
BceAI	ACGGC	2 cut(s)	194, 440
BciT130I	CCWGG	3 cut(s)	173, 1198, 1217
BciVI	GTATCC	1 cut(s)	1281
BclI	TGATCA	1 cut(s)	744
BcnI	CCSGG	1 cut(s)	765
BcoDI	GTCTC	3 cut(s)	793, 1094, 1681
BfaI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
BfmI	CTRYAG	1 cut(s)	1682
BfoI	RGCGCY	2 cut(s)	318, 1737
BfuI	GTATCC	1 cut(s)	1281
BisI	GCNGC	8 cut(s)	438, 1302, 1365, 1452, 1508, 1941, 2019, 2142
BlsI	GCNGC	8 cut(s)	439, 1303, 1366, 1453, 1509, 1942, 2020, 2143
BmcAI	AGTACT	1 cut(s)	825
Bme1390I	CCNGG	4 cut(s)	173, 765, 1198, 1217
Bme18I	GGWCC	1 cut(s)	52
BmgT120I	GGNCC	1 cut(s)	52
BmiI	GGNNCC	3 cut(s)	1488, 1650, 1839
BmrFI	CCNGG	4 cut(s)	173, 765, 1198, 1217
BmsI	GCATC	7 cut(s)	959, 1036, 1142, 1202, 1288, 1450, 1971
BpiI	GAAGAC	1 cut(s)	1211
BplI	GAGNNNNNCTC	4 cut(s)	1583, 1615, 1708, 1740
BpmI	CTGGAG	2 cut(s)	1296, 2027
Bpu10I	CCTNAGC	2 cut(s)	56, 527
Bpu14I	TTCGAA	1 cut(s)	1028
BpuMI	CCSGG	1 cut(s)	765
BsaBI	GATNNNNATC	1 cut(s)	486
BsaHI	GRCGYC	3 cut(s)	6, 207, 240
BsaJI	CCNNGG	3 cut(s)	441, 992, 1274
BsaWI	WCCGGW	1 cut(s)	85
BsaXI	ACNNNNNCTCC	6 cut(s)	75, 105, 176, 206, 771, 801
Bsc4I	CCNNNNNNNGG	2 cut(s)	280, 1761
Bse1I	ACTGG	3 cut(s)	509, 1153, 2270
Bse3DI	GCAATG	1 cut(s)	2049
Bse8I	GATNNNNATC	1 cut(s)	486
BseBI	CCWGG	3 cut(s)	173, 1198, 1217
BseDI	CCNNGG	3 cut(s)	441, 992, 1274
BseGI	GGATG	9 cut(s)	102, 700, 889, 1193, 1303, 1411, 1418, 1465, 2022
BseJI	GATNNNNATC	1 cut(s)	486
BseLI	CCNNNNNNNGG	2 cut(s)	280, 1761
BseMI	GCAATG	1 cut(s)	2049
BseMII	CTCAG	8 cut(s)	70, 245, 419, 440, 518, 822, 1580, 1614
BseNI	ACTGG	3 cut(s)	509, 1153, 2270
BseRI	GAGGAG	2 cut(s)	89, 149
BseSI	GKGCMC	1 cut(s)	1392
BseXI	GCAGC	7 cut(s)	1288, 1376, 1438, 1519, 1952, 2005, 2153
Bsh1236I	CGCG	2 cut(s)	217, 401
Bsh1285I	CGRYCG	2 cut(s)	271, 279
BshFI	GGCC	1 cut(s)	991
BsiEI	CGRYCG	2 cut(s)	271, 279
BsiHKAI	GWGCWC	4 cut(s)	39, 672, 784, 1392
BsiSI	CCGG	2 cut(s)	86, 764
BslFI	GGGAC	1 cut(s)	1996
BslI	CCNNNNNNNGG	2 cut(s)	280, 1761
BsmAI	GTCTC	3 cut(s)	793, 1094, 1681
BsmFI	GGGAC	1 cut(s)	1996
BsmI	GAATGC	2 cut(s)	551, 2246
BsnI	GGCC	1 cut(s)	991
Bsp119I	TTCGAA	1 cut(s)	1028
Bsp1286I	GDGCHC	4 cut(s)	39, 672, 784, 1392
Bsp1407I	TGTACA	1 cut(s)	516
BspACI	CCGC	9 cut(s)	81, 94, 271, 284, 438, 593, 598, 607, 673
BspANI	GGCC	1 cut(s)	991
BspCNI	CTCAG	8 cut(s)	69, 246, 420, 441, 519, 823, 1579, 1613
BspFNI	CGCG	2 cut(s)	217, 401
BspHI	TCATGA	1 cut(s)	1264
BspLI	GGNNCC	3 cut(s)	1488, 1650, 1839
BspMAI	CTGCAG	1 cut(s)	1686
BspPI	GGATC	3 cut(s)	96, 857, 1207
BspT104I	TTCGAA	1 cut(s)	1028
BsrBI	CCGCTC	1 cut(s)	607
BsrDI	GCAATG	1 cut(s)	2049
BsrGI	TGTACA	1 cut(s)	516
BsrI	ACTGG	3 cut(s)	509, 1153, 2270
BssECI	CCNNGG	3 cut(s)	441, 992, 1274
BssNI	GRCGYC	3 cut(s)	6, 207, 240
BssT1I	CCWWGG	3 cut(s)	441, 992, 1274
Bst2UI	CCWGG	3 cut(s)	173, 1198, 1217
Bst4CI	ACNGT	1 cut(s)	679
Bst6I	CTCTTC	2 cut(s)	194, 1266
BstACI	GRCGYC	3 cut(s)	6, 207, 240
BstAPI	GCANNNNNTGC	1 cut(s)	2055
BstAUI	TGTACA	1 cut(s)	516
BstBI	TTCGAA	1 cut(s)	1028
BstC8I	GCNNGC	3 cut(s)	524, 1584, 2056
BstDEI	CTNAG	9 cut(s)	56, 254, 428, 449, 470, 527, 831, 1566, 1600
BstF5I	GGATG	9 cut(s)	102, 700, 889, 1193, 1303, 1411, 1418, 1465, 2022
BstFNI	CGCG	2 cut(s)	217, 401
BstH2I	RGCGCY	2 cut(s)	318, 1737
BstHHI	GCGC	6 cut(s)	49, 219, 317, 401, 1736, 2166
BstMAI	GTCTC	3 cut(s)	793, 1094, 1681
BstMCI	CGRYCG	2 cut(s)	271, 279
BstMWI	GCNNNNNNNGC	8 cut(s)	374, 604, 731, 1460, 1504, 2055, 2172, 2216
BstNI	CCWGG	3 cut(s)	173, 1198, 1217
BstNSI	RCATGY	3 cut(s)	755, 1631, 1703
BstSCI	CCNGG	4 cut(s)	171, 763, 1196, 1215
BstSFI	CTRYAG	1 cut(s)	1682
BstSLI	GKGCMC	1 cut(s)	1392
BstUI	CGCG	2 cut(s)	217, 401
BstV1I	GCAGC	7 cut(s)	1288, 1376, 1438, 1519, 1952, 2005, 2153
BstV2I	GAAGAC	1 cut(s)	1211
BstX2I	RGATCY	2 cut(s)	88, 1212
BstYI	RGATCY	2 cut(s)	88, 1212
BsuI	GTATCC	1 cut(s)	1281
BsuRI	GGCC	1 cut(s)	991
BtsCI	GGATG	9 cut(s)	102, 700, 889, 1193, 1303, 1411, 1418, 1465, 2022
BtsIMutI	CAGTG	1 cut(s)	516
Cac8I	GCNNGC	3 cut(s)	524, 1584, 2056
CaiI	CAGNNNCTG	2 cut(s)	253, 1136
CciI	TCATGA	1 cut(s)	1264
CfoI	GCGC	6 cut(s)	49, 219, 317, 401, 1736, 2166
Cfr13I	GGNCC	1 cut(s)	52
CseI	GACGC	2 cut(s)	14, 215
Csp6I	GTAC	5 cut(s)	517, 638, 824, 1086, 1823
CviAII	CATG	7 cut(s)	752, 987, 1265, 1526, 1628, 1700, 1757
CviQI	GTAC	5 cut(s)	517, 638, 824, 1086, 1823
DdeI	CTNAG	9 cut(s)	56, 254, 428, 449, 470, 527, 831, 1566, 1600
DrdI	GACNNNNNNGTC	1 cut(s)	76
DseDI	GACNNNNNNGTC	1 cut(s)	76
EaeI	YGGCCR	1 cut(s)	989
Eam1104I	CTCTTC	2 cut(s)	194, 1266
EarI	CTCTTC	2 cut(s)	194, 1266
EciI	GGCGGA	2 cut(s)	83, 299
Ecl136II	GAGCTC	3 cut(s)	37, 670, 782
Eco130I	CCWWGG	3 cut(s)	441, 992, 1274
Eco24I	GRGCYC	3 cut(s)	39, 672, 784
Eco47I	GGWCC	1 cut(s)	52
Eco47III	AGCGCT	1 cut(s)	316
Eco53kI	GAGCTC	3 cut(s)	37, 670, 782
Eco57I	CTGAAG	4 cut(s)	479, 822, 1062, 1385
EcoICRI	GAGCTC	3 cut(s)	37, 670, 782
EcoO109I	RGGNCCY	1 cut(s)	52
EcoRII	CCWGG	3 cut(s)	171, 1196, 1215
EcoT14I	CCWWGG	3 cut(s)	441, 992, 1274
EcoT38I	GRGCYC	3 cut(s)	39, 672, 784
ErhI	CCWWGG	3 cut(s)	441, 992, 1274
FaeI	CATG	7 cut(s)	755, 990, 1268, 1529, 1631, 1703, 1760
FalI	AAGNNNNNCTT	2 cut(s)	555, 587
FaqI	GGGAC	1 cut(s)	1996
FatI	CATG	7 cut(s)	751, 986, 1264, 1525, 1627, 1699, 1756
FauI	CCCGC	1 cut(s)	600
FbaI	TGATCA	1 cut(s)	744
Fnu4HI	GCNGC	8 cut(s)	438, 1302, 1365, 1452, 1508, 1941, 2019, 2142
FokI	GGATG	9 cut(s)	89, 707, 896, 1180, 1310, 1405, 1418, 1472, 2009
FriOI	GRGCYC	3 cut(s)	39, 672, 784
Fsp4HI	GCNGC	8 cut(s)	438, 1302, 1365, 1452, 1508, 1941, 2019, 2142
FspBI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
FspI	TGCGCA	1 cut(s)	2165
GlaI	GCGC	6 cut(s)	48, 218, 316, 400, 1735, 2165
GluI	GCNGC	8 cut(s)	438, 1302, 1365, 1452, 1508, 1941, 2019, 2142
GsuI	CTGGAG	2 cut(s)	1296, 2027
HaeII	RGCGCY	2 cut(s)	318, 1737
HaeIII	GGCC	1 cut(s)	991
HapII	CCGG	2 cut(s)	86, 764
HgaI	GACGC	2 cut(s)	14, 215
HhaI	GCGC	6 cut(s)	49, 219, 317, 401, 1736, 2166
Hin1I	GRCGYC	3 cut(s)	6, 207, 240
Hin1II	CATG	7 cut(s)	755, 990, 1268, 1529, 1631, 1703, 1760
Hin6I	GCGC	6 cut(s)	47, 217, 315, 399, 1734, 2164
HinP1I	GCGC	6 cut(s)	47, 217, 315, 399, 1734, 2164
HincII	GTYRAC	1 cut(s)	1324
HindII	GTYRAC	1 cut(s)	1324
HindIII	AAGCTT	1 cut(s)	687
HpaII	CCGG	2 cut(s)	86, 764
HphI	GGTGA	4 cut(s)	158, 373, 753, 2217
Hpy166II	GTNNAC	4 cut(s)	517, 1186, 1324, 1390
Hpy8I	GTNNAC	4 cut(s)	517, 1186, 1324, 1390
Hpy99I	CGWCG	6 cut(s)	209, 239, 242, 245, 325, 2185
HpyAV	CCTTC	6 cut(s)	337, 526, 956, 1024, 1863, 2088
HpyCH4III	ACNGT	1 cut(s)	679
HpyCH4IV	ACGT	4 cut(s)	27, 240, 1825, 2180
HpyCH4V	TGCA	8 cut(s)	201, 1193, 1390, 1543, 1684, 1781, 2058, 2219
HpyF10VI	GCNNNNNNNGC	8 cut(s)	374, 604, 731, 1460, 1504, 2055, 2172, 2216
HpyF3I	CTNAG	9 cut(s)	56, 254, 428, 449, 470, 527, 831, 1566, 1600
HpySE526I	ACGT	4 cut(s)	27, 240, 1825, 2180
Hsp92I	GRCGYC	3 cut(s)	6, 207, 240
Hsp92II	CATG	7 cut(s)	755, 990, 1268, 1529, 1631, 1703, 1760
HspAI	GCGC	6 cut(s)	47, 217, 315, 399, 1734, 2164
Ksp22I	TGATCA	1 cut(s)	744
LmnI	GCTCC	9 cut(s)	42, 132, 224, 454, 675, 779, 787, 1654, 1837
Lsp1109I	GCAGC	7 cut(s)	1288, 1376, 1438, 1519, 1952, 2005, 2153
LweI	GCATC	7 cut(s)	959, 1036, 1142, 1202, 1288, 1450, 1971
MaeI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
MaeII	ACGT	4 cut(s)	27, 240, 1825, 2180
MaeIII	GTNAC	3 cut(s)	211, 1906, 2037
MbiI	CCGCTC	1 cut(s)	607
MfeI	CAATTG	1 cut(s)	1368
MflI	RGATCY	2 cut(s)	88, 1212
MhlI	GDGCHC	4 cut(s)	39, 672, 784, 1392
MlsI	TGGCCA	1 cut(s)	991
MluNI	TGGCCA	1 cut(s)	991
MlyI	GAGTC	3 cut(s)	11, 110, 2018
MmeI	TCCRAC	5 cut(s)	130, 258, 291, 333, 682
Mox20I	TGGCCA	1 cut(s)	991
MroXI	GAANNNNTTC	1 cut(s)	549
MscI	TGGCCA	1 cut(s)	991
MseI	TTAA	3 cut(s)	1007, 1109, 2126
Msp20I	TGGCCA	1 cut(s)	991
MspI	CCGG	2 cut(s)	86, 764
MspR9I	CCNGG	4 cut(s)	173, 765, 1198, 1217
MunI	CAATTG	1 cut(s)	1368
Mva1269I	GAATGC	2 cut(s)	551, 2246
MvaI	CCWGG	3 cut(s)	173, 1198, 1217
MvnI	CGCG	2 cut(s)	217, 401
MwoI	GCNNNNNNNGC	8 cut(s)	374, 604, 731, 1460, 1504, 2055, 2172, 2216
NciI	CCSGG	1 cut(s)	765
NlaIII	CATG	7 cut(s)	755, 990, 1268, 1529, 1631, 1703, 1760
NlaIV	GGNNCC	3 cut(s)	1488, 1650, 1839
NmuCI	GTSAC	2 cut(s)	1906, 2037
NsbI	TGCGCA	1 cut(s)	2165
NspI	RCATGY	3 cut(s)	755, 1631, 1703
NspV	TTCGAA	1 cut(s)	1028
PagI	TCATGA	1 cut(s)	1264
PciI	ACATGT	1 cut(s)	1699
PcsI	WCGNNNNNNNCGW	2 cut(s)	12, 329
PctI	GAATGC	2 cut(s)	551, 2246
PdmI	GAANNNNTTC	1 cut(s)	549
PflFI	GACNNNGTC	1 cut(s)	239
PfoI	TCCNGGA	1 cut(s)	1196
PkrI	GCNGC	8 cut(s)	439, 1303, 1366, 1453, 1509, 1942, 2020, 2143
Ple19I	CGATCG	1 cut(s)	279
PleI	GAGTC	3 cut(s)	11, 110, 2017
PpsI	GAGTC	3 cut(s)	11, 110, 2017
PpuMI	RGGWCCY	1 cut(s)	52
PscI	ACATGT	1 cut(s)	1699
PsiI	TTATAA	1 cut(s)	302
Psp124BI	GAGCTC	3 cut(s)	39, 672, 784
Psp1406I	AACGTT	1 cut(s)	27
Psp5II	RGGWCCY	1 cut(s)	52
Psp6I	CCWGG	3 cut(s)	171, 1196, 1215
PspGI	CCWGG	3 cut(s)	171, 1196, 1215
PspN4I	GGNNCC	3 cut(s)	1488, 1650, 1839
PspPI	GGNCC	1 cut(s)	52
PspPPI	RGGWCCY	1 cut(s)	52
PstI	CTGCAG	1 cut(s)	1686
PstNI	CAGNNNCTG	2 cut(s)	253, 1136
PsuI	RGATCY	2 cut(s)	88, 1212
PsyI	GACNNNGTC	1 cut(s)	239
PvuI	CGATCG	1 cut(s)	279
RsaI	GTAC	5 cut(s)	518, 639, 825, 1087, 1824
RsaNI	GTAC	5 cut(s)	517, 638, 824, 1086, 1823
SacI	GAGCTC	3 cut(s)	39, 672, 784
SaqAI	TTAA	3 cut(s)	1007, 1109, 2126
SatI	GCNGC	8 cut(s)	438, 1302, 1365, 1452, 1508, 1941, 2019, 2142
Sau96I	GGNCC	1 cut(s)	52
ScaI	AGTACT	1 cut(s)	825
SchI	GAGTC	3 cut(s)	11, 110, 2018
ScrFI	CCNGG	4 cut(s)	173, 765, 1198, 1217
SduI	GDGCHC	4 cut(s)	39, 672, 784, 1392
SfaNI	GCATC	7 cut(s)	959, 1036, 1142, 1202, 1288, 1450, 1971
SfcI	CTRYAG	1 cut(s)	1682
SfuI	TTCGAA	1 cut(s)	1028
SinI	GGWCC	1 cut(s)	52
SsiI	CCGC	9 cut(s)	81, 94, 271, 284, 438, 593, 598, 607, 673
SspMI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
SstI	GAGCTC	3 cut(s)	39, 672, 784
StyD4I	CCNGG	4 cut(s)	171, 763, 1196, 1215
StyI	CCWWGG	3 cut(s)	441, 992, 1274
TaaI	ACNGT	1 cut(s)	679
TaiI	ACGT	4 cut(s)	30, 243, 1828, 2183
TatI	WGTACW	4 cut(s)	516, 637, 823, 1085
TauI	GCSGC	1 cut(s)	440
Tru1I	TTAA	3 cut(s)	1007, 1109, 2126
Tru9I	TTAA	3 cut(s)	1007, 1109, 2126
TscAI	CASTG	1 cut(s)	516
TseFI	GTSAC	2 cut(s)	1906, 2037
TseI	GCWGC	7 cut(s)	1301, 1364, 1451, 1507, 1940, 2018, 2141
Tsp45I	GTSAC	2 cut(s)	1906, 2037
TspDTI	ATGAA	9 cut(s)	571, 969, 1133, 1253, 1394, 1491, 1773, 1964, 2062
TspGWI	ACGGA	2 cut(s)	184, 614
TspRI	CASTG	1 cut(s)	516
Tth111I	GACNNNGTC	1 cut(s)	239
VneI	GTGCAC	1 cut(s)	1388
VpaK11BI	GGWCC	1 cut(s)	52
XapI	RAATTY	7 cut(s)	705, 772, 1019, 1094, 1692, 1783, 2091
XbaI	TCTAGA	2 cut(s)	1726, 1901
XceI	RCATGY	3 cut(s)	755, 1631, 1703
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1432
XmnI	GAANNNNTTC	1 cut(s)	549
XspI	CTAG	5 cut(s)	1385, 1455, 1499, 1727, 1902
ZraI	GACGTC	1 cut(s)	241
ZrmI	AGTACT	1 cut(s)	825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.