AT2G34460

At2g34460

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008667
Physical Location & Seq
Forward (+)
14529558 .. 14530958
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G34460.1

Sequence Viewer

Length: 843 bp
ATGGCAACATCTTTGCTTCTCAGACATTCCTCAGCTGTCTTCTTCTCTCAGTCTTCCTTCTTCACCAAGAACAAATCTTTTCGCTCCTTTACTTCCATCAAAATGGAAAAAGGTGAAGCAGAGAATGCAGTGAAGACAAAGAAGGTGTTTGTCGCAGGAGCAACTGGACAAACCGGGAAGAGGATTGTGGAGCAGTTGTTGTCCCGTGGCTTTGCCGTCAAAGCTGGTGTTCGTGATGTAGAGAAAGCAAAAACATCTTTCAAAGACGACCCGTCTCTTCAGATAGTTAGAGCAGACGTGACAGAAGGTCCTGATAAGCTAGCTGAAGTTATAGGTGATGATTCTCAAGCTGTGATTTGTGCTACTGGGTTTCGTCCCGGATTTGATATATTCACTCCTTGGAAAGTCGATAATTTCGGGACAGTGAATCTTGTGGATGCTTGCCGTAAACAAGGAGTGGAAAAGTTTGTTCTTGTAAGCTCCATCTTGGTGAATGGAGCTGCCATGGGACAGATACTGAACCCTGCTTACCTCTTCCTCAACCTCTTTGGACTAACTCTTGTCGCTAAGCTTCAAGCCGAGAAGTATATCAAAAAATCTGGCATCAACTATACTATAGTAAGGCCTGGTGGTCTTAAAAACGATCCTCCAACTGGAAATGTTGTCATGGAGCCAGAGGATACTCTATATGAAGGGAGTATATCGAGAGACCTGGTCGCAGAAGTTGCAGTGGAAGCTTTACTTCAAGAGGAATCATCTTTCAAGGTTGTGGAGATAGTTGCTCGTGCCGAAGCTCCAAAACGTTCCTACAAAGATCTATTTGCCTCTGTTAAAGGACAATAA

Protein Analysis

280

Amino Acids

30.47

Weight (kDa)

9.04

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 46 - 209 2.8e-15 NmrA-like family
Epimerase PF01370 49 - 209 1.8e-11 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 51 - 173 1.7e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 53 - 249 3.3e-46 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 802
AclWI GGATC 1 cut(s) 638
AcuI CTGAAG 2 cut(s) 263, 345
AfiI CCNNNNNNNGG 2 cut(s) 180, 653
AgsI TTSAA 4 cut(s) 262, 575, 746, 763
AhdI GACNNNNNGTC 2 cut(s) 271, 306
AjiI CACGTC 1 cut(s) 298
AjnI CCWGG 2 cut(s) 625, 711
Alw26I GTCTC 2 cut(s) 279, 702
AlwI GGATC 1 cut(s) 638
AlwNI CAGNNNCTG 1 cut(s) 517
AoxI GGCC 1 cut(s) 623
ApeKI GCWGC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 2 cut(s) 175, 378
AsuHPI GGTGA 4 cut(s) 55, 125, 347, 502
AsuNHI GCTAGC 1 cut(s) 319
AvaII GGWCC 1 cut(s) 308
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 3 cut(s) 31, 45, 140
BbvCI CCTCAGC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 487
BccI CCATC 2 cut(s) 104, 491
BceAI ACGGC 2 cut(s) 200, 429
BciT130I CCWGG 2 cut(s) 627, 713
BciVI GTATCC 1 cut(s) 673
BcnI CCSGG 2 cut(s) 175, 378
BcoDI GTCTC 2 cut(s) 279, 702
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 1 cut(s) 615
BfuI GTATCC 1 cut(s) 673
BglII AGATCT 1 cut(s) 814
BisI GCNGC 1 cut(s) 501
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 4 cut(s) 175, 378, 627, 713
Bme18I GGWCC 1 cut(s) 308
BmeRI GACNNNNNGTC 2 cut(s) 271, 306
BmgBI CACGTC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 1 cut(s) 672
BmrFI CCNGG 4 cut(s) 175, 378, 627, 713
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 2 cut(s) 427, 612
BmtI GCTAGC 1 cut(s) 323
BmuI ACTGGG 1 cut(s) 375
BpiI GAAGAC 3 cut(s) 31, 45, 140
Bpu10I CCTNAGC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 2 cut(s) 175, 378
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 3 cut(s) 205, 398, 504
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 653
Bse1I ACTGG 3 cut(s) 169, 370, 658
BseBI CCWGG 2 cut(s) 627, 713
BseDI CCNNGG 3 cut(s) 205, 398, 504
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 2 cut(s) 180, 653
BseMII CTCAG 3 cut(s) 34, 45, 62
BseNI ACTGG 3 cut(s) 169, 370, 658
BseXI GCAGC 1 cut(s) 487
BshFI GGCC 1 cut(s) 625
BsiSI CCGG 2 cut(s) 174, 378
BslFI GGGAC 4 cut(s) 187, 360, 433, 522
BslI CCNNNNNNNGG 2 cut(s) 180, 653
BsmAI GTCTC 2 cut(s) 279, 702
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 4 cut(s) 187, 360, 433, 522
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 1 cut(s) 625
Bso31I GGTCTC 1 cut(s) 702
Bsp143I GATC 2 cut(s) 643, 814
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 504
BspANI GGCC 1 cut(s) 625
BspCNI CTCAG 3 cut(s) 33, 44, 61
BspLI GGNNCC 1 cut(s) 672
BspOI GCTAGC 1 cut(s) 323
BspPI GGATC 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 702
BsrI ACTGG 3 cut(s) 169, 370, 658
BssECI CCNNGG 3 cut(s) 205, 398, 504
BssMI GATC 2 cut(s) 643, 814
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 2 cut(s) 398, 504
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 627, 713
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 3 cut(s) 173, 282, 539
BstAPI GCANNNNNTGC 2 cut(s) 125, 725
BstC8I GCNNGC 2 cut(s) 321, 442
BstDEI CTNAG 4 cut(s) 20, 31, 48, 567
BstDSI CCRYGG 2 cut(s) 205, 504
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 2 cut(s) 646, 817
BstMAI GTCTC 2 cut(s) 279, 702
BstMBI GATC 2 cut(s) 643, 814
BstMWI GCNNNNNNNGC 4 cut(s) 125, 221, 725, 734
BstNI CCWGG 2 cut(s) 627, 713
BstSCI CCNGG 4 cut(s) 173, 376, 625, 711
BstSFI CTRYAG 1 cut(s) 615
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 3 cut(s) 31, 45, 140
BstX2I RGATCY 1 cut(s) 814
BstXI CCANNNNNNTGG 1 cut(s) 103
BstYI RGATCY 1 cut(s) 814
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 1 cut(s) 625
BtgI CCRYGG 2 cut(s) 205, 504
BtrI CACGTC 1 cut(s) 298
BtsCI GGATG 1 cut(s) 442
BtsI GCAGTG 2 cut(s) 135, 735
BtsIMutI CAGTG 3 cut(s) 135, 429, 735
Cac8I GCNNGC 2 cut(s) 321, 442
CaiI CAGNNNCTG 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 308
CsiI ACCWGGT 1 cut(s) 711
CviAII CATG 2 cut(s) 505, 667
DdeI CTNAG 4 cut(s) 20, 31, 48, 567
DpnI GATC 2 cut(s) 645, 816
DpnII GATC 2 cut(s) 643, 814
DriI GACNNNNNGTC 2 cut(s) 271, 306
Eam1104I CTCTTC 3 cut(s) 173, 282, 539
Eam1105I GACNNNNNGTC 2 cut(s) 271, 306
EarI CTCTTC 3 cut(s) 173, 282, 539
Eco130I CCWWGG 2 cut(s) 398, 504
Eco147I AGGCCT 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 702
Eco47I GGWCC 1 cut(s) 308
Eco57I CTGAAG 2 cut(s) 263, 345
EcoO109I RGGNCCY 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 625, 711
EcoT14I CCWWGG 2 cut(s) 398, 504
ErhI CCWWGG 2 cut(s) 398, 504
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 2 cut(s) 508, 670
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 4 cut(s) 187, 360, 433, 522
FatI CATG 2 cut(s) 504, 666
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 1 cut(s) 320
GluI GCNGC 1 cut(s) 501
HaeIII GGCC 1 cut(s) 625
HapII CCGG 2 cut(s) 174, 378
Hin1II CATG 2 cut(s) 508, 670
HindIII AAGCTT 2 cut(s) 569, 735
HinfI GANTC 3 cut(s) 341, 427, 752
HpaII CCGG 2 cut(s) 174, 378
HphI GGTGA 4 cut(s) 55, 125, 347, 502
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 23, 282
Hpy188III TCNNGA 5 cut(s) 233, 311, 418, 705, 746
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 4 cut(s) 67, 136, 299, 686
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 2 cut(s) 297, 802
HpyCH4V TGCA 2 cut(s) 128, 728
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 221, 725, 734
HpyF3I CTNAG 4 cut(s) 20, 31, 48, 567
HpySE526I ACGT 2 cut(s) 297, 802
Hsp92II CATG 2 cut(s) 508, 670
Kzo9I GATC 2 cut(s) 643, 814
LmnI GCTCC 7 cut(s) 89, 158, 190, 485, 497, 670, 799
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 2 cut(s) 427, 612
MabI ACCWGGT 1 cut(s) 711
MaeI CTAG 1 cut(s) 320
MaeII ACGT 2 cut(s) 297, 802
MaeIII GTNAC 1 cut(s) 298
MalI GATC 2 cut(s) 645, 816
MboI GATC 2 cut(s) 643, 814
MboII GAAGA 8 cut(s) 31, 34, 45, 52, 145, 190, 269, 526
MflI RGATCY 1 cut(s) 814
MluCI AATT 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 674
MnlI CCTC 9 cut(s) 40, 174, 542, 548, 554, 657, 670, 742, 835
MseI TTAA 2 cut(s) 636, 831
MslI CAYNNNNRTG 2 cut(s) 101, 488
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 2 cut(s) 174, 378
MspR9I CCNGG 4 cut(s) 175, 378, 627, 713
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 2 cut(s) 627, 713
MwoI GCNNNNNNNGC 4 cut(s) 125, 221, 725, 734
NciI CCSGG 2 cut(s) 175, 378
NcoI CCATGG 1 cut(s) 504
NdeII GATC 2 cut(s) 643, 814
NheI GCTAGC 1 cut(s) 319
NlaIII CATG 2 cut(s) 508, 670
NlaIV GGNNCC 1 cut(s) 672
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 625
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 3 cut(s) 341, 427, 752
PflFI GACNNNGTC 1 cut(s) 713
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 1 cut(s) 502
PpuMI RGGWCCY 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 802
Psp5II RGGWCCY 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 625, 711
PspGI CCWGG 2 cut(s) 625, 711
PspN4I GGNNCC 1 cut(s) 672
PspPI GGNCC 1 cut(s) 308
PspPPI RGGWCCY 1 cut(s) 308
PsrI GAACNNNNNNTAC 2 cut(s) 512, 544
PstNI CAGNNNCTG 1 cut(s) 517
PsuI RGATCY 1 cut(s) 814
PsyI GACNNNGTC 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 35
RseI CAYNNNNRTG 2 cut(s) 101, 488
SaqAI TTAA 2 cut(s) 636, 831
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 2 cut(s) 643, 814
Sau96I GGNCC 1 cut(s) 308
ScrFI CCNGG 4 cut(s) 175, 378, 627, 713
SexAI ACCWGGT 1 cut(s) 711
SfaNI GCATC 2 cut(s) 427, 612
SfcI CTRYAG 1 cut(s) 615
SinI GGWCC 1 cut(s) 308
SmiMI CAYNNNNRTG 2 cut(s) 101, 488
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 1 cut(s) 412
SseBI AGGCCT 1 cut(s) 625
SspMI CTAG 1 cut(s) 320
StuI AGGCCT 1 cut(s) 625
StyD4I CCNGG 4 cut(s) 173, 376, 625, 711
StyI CCWWGG 2 cut(s) 398, 504
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 2 cut(s) 300, 805
TaqI TCGA 2 cut(s) 408, 704
TasI AATT 1 cut(s) 412
TfiI GAWTC 3 cut(s) 341, 427, 752
Tru1I TTAA 2 cut(s) 636, 831
Tru9I TTAA 2 cut(s) 636, 831
TscAI CASTG 3 cut(s) 135, 429, 735
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 1 cut(s) 705
TspRI CASTG 3 cut(s) 135, 429, 735
Tth111I GACNNNGTC 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 308
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.