Rh5BG102600

protein At2g34460, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008667
Physical Location & Seq
Forward (+)
9358498 .. 9405635
47138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG102600.1

Sequence Viewer

Length: 981 bp
ATGGCAGCTCCGCTGCCTCTCATTCTCAGAAACCCTTCTTCTCTCTTAGCTCCGATCCCGACGAAGCCTCCTCTCCGTCTCCCCAACACTCACTCCCACAGCCACCACTACTCCCCTCTCTCCACAACCAAACCCTTTTCCGTCATTTCACTCTCCTCCTCCTCCTCCACCACCAGGCCCTTATTCCCCATCCTTAACTCCTCTAAGATGGAAGGGAGTGAAACCACTGGGAATGAAATCACAGAGGAAGTTACTGAGACCACAACTGAAGCAACAAGTGTGAATGGTTTGAAGAAGATTTTTGTGGCTGGTGCTACTGGGAGCACTGGTAAAAGAATTGTTGAGCAGTTACTGGCTAGGGGTTATGGAGTAAAAGCCGGAGTTCGGGACTTGGAAAAAGCCAAAACTAGTCTTCCAAGCAACAACCCAGCTCTTGAGATTGTGAAAGCAGATGTGACAGAGGGTTCTGCGAAGTTGGCAGAAGCAACTAGTAGTGATTCGGAAGCTGTGATTTGCGCTACGGGGTTTAGCTATGGATGGGATGTTGATAATTTTGGGACAGTTAACCTTGTCGATGCATGCCGTAAAGTTGGTGTGAAGAGGTTTATTCTGGTCAGTTCCATTTTAGTCAATGGAGCATCAATGGGGCAGATTCTCAATCCGGCTTACATTTTTCTTAATATATTTGGACTCACTTTAATCGCAAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGTATAAACTACACAATAATAAGGCCTGGTGGGTTGAGAAATGAACCTCCAACAGGGAATCTTGTGATGGAGCCAGAGGACACTCTATCTGGAGGCACCATCTCCAGAGATCTGGTTGCGGAAGTAGCTGTCGAGGCATTGGTCACTCCTGAAGCATCTTACAAGGTAGTTGAGATAGTTTCCAGTGCTGATGCCCCCAAGCGTACATACAAGGAACTATTTGGTTCGGTTAAGCAACGGTGA

Protein Analysis

326

Amino Acids

34.73

Weight (kDa)

8.4

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 98 - 253 6.5e-19 NmrA-like family
Epimerase PF01370 100 - 255 1.1e-10 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 102 - 218 3e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 104 - 295 2.3e-45 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 833
AciI CCGC 2 cut(s) 11, 857
AclWI GGATC 1 cut(s) 49
AcuI CTGAAG 3 cut(s) 288, 695, 909
AfaI GTAC 1 cut(s) 943
AfiI CCNNNNNNNGG 3 cut(s) 174, 384, 791
AgsI TTSAA 1 cut(s) 292
AhlI ACTAGT 2 cut(s) 407, 488
AjnI CCWGG 2 cut(s) 173, 763
AluBI AGCT 7 cut(s) 8, 50, 431, 506, 531, 709, 866
AluI AGCT 7 cut(s) 8, 50, 431, 506, 531, 709, 866
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 2 cut(s) 83, 251
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 352
AoxI GGCC 2 cut(s) 176, 761
ApeKI GCWGC 2 cut(s) 5, 13
Asp700I GAANNNNTTC 1 cut(s) 34
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 833
BbsI GAAGAC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 1 cut(s) 17
BccI CCATC 5 cut(s) 197, 202, 531, 799, 845
BceAI ACGGC 1 cut(s) 567
BciT130I CCWGG 2 cut(s) 175, 765
BcoDI GTCTC 2 cut(s) 83, 251
BcuI ACTAGT 2 cut(s) 407, 488
BfaI CTAG 3 cut(s) 357, 408, 489
BglII AGATCT 1 cut(s) 847
BisI GCNGC 2 cut(s) 6, 14
BlsI GCNGC 2 cut(s) 7, 15
Bme1390I CCNGG 2 cut(s) 175, 765
BmgT120I GGNCC 1 cut(s) 177
BmiI GGNNCC 2 cut(s) 810, 835
BmrFI CCNGG 2 cut(s) 175, 765
BmrI ACTGGG 2 cut(s) 237, 327
BmsI GCATC 4 cut(s) 565, 647, 902, 919
BmuI ACTGGG 2 cut(s) 237, 327
BpiI GAAGAC 1 cut(s) 404
BpmI CTGGAG 2 cut(s) 826, 849
BpuEI CTTGAG 1 cut(s) 455
BsaI GGTCTC 1 cut(s) 251
BsaXI ACNNNNNCTCC 8 cut(s) 52, 77, 82, 95, 107, 125, 313, 343
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 384, 791
Bse1I ACTGG 5 cut(s) 232, 322, 331, 357, 921
BseBI CCWGG 2 cut(s) 175, 765
BseGI GGATG 3 cut(s) 189, 542, 547
BseLI CCNNNNNNNGG 3 cut(s) 174, 384, 791
BseMII CTCAG 2 cut(s) 40, 246
BseNI ACTGG 5 cut(s) 232, 322, 331, 357, 921
BseRI GAGGAG 6 cut(s) 60, 145, 148, 151, 154, 190
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 2 cut(s) 178, 763
BshNI GGYRCC 1 cut(s) 833
BsiHKAI GWGCWC 1 cut(s) 326
BsiSI CCGG 2 cut(s) 378, 662
BslFI GGGAC 2 cut(s) 401, 571
BslI CCNNNNNNNGG 3 cut(s) 174, 384, 791
BsmAI GTCTC 2 cut(s) 83, 251
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 401, 571
BsnI GGCC 2 cut(s) 178, 763
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 2 cut(s) 54, 847
BspACI CCGC 2 cut(s) 11, 857
BspANI GGCC 2 cut(s) 178, 763
BspCNI CTCAG 2 cut(s) 39, 247
BspLI GGNNCC 2 cut(s) 810, 835
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 5 cut(s) 232, 322, 331, 357, 921
BssMI GATC 2 cut(s) 54, 847
Bst2UI CCWGG 2 cut(s) 175, 765
Bst4CI ACNGT 2 cut(s) 562, 978
Bst6I CTCTTC 1 cut(s) 593
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 4 cut(s) 26, 46, 204, 255
BstENI CCTNNNNNAGG 1 cut(s) 789
BstF5I GGATG 3 cut(s) 189, 542, 547
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 2 cut(s) 57, 850
BstMAI GTCTC 2 cut(s) 83, 251
BstMBI GATC 2 cut(s) 54, 847
BstMWI GCNNNNNNNGC 3 cut(s) 476, 863, 872
BstNI CCWGG 2 cut(s) 175, 765
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 173, 763
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 404
BstX2I RGATCY 1 cut(s) 847
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 1 cut(s) 847
BsuRI GGCC 2 cut(s) 178, 763
BtsCI GGATG 3 cut(s) 189, 542, 547
BtsIMutI CAGTG 3 cut(s) 225, 324, 928
Cac8I GCNNGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 352
CfoI GCGC 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 177
Csp6I GTAC 1 cut(s) 942
CviAII CATG 1 cut(s) 579
CviQI GTAC 1 cut(s) 942
DdeI CTNAG 4 cut(s) 26, 46, 204, 255
DpnI GATC 2 cut(s) 56, 849
DpnII GATC 2 cut(s) 54, 847
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Eco147I AGGCCT 1 cut(s) 763
Eco31I GGTCTC 1 cut(s) 251
Eco57I CTGAAG 3 cut(s) 288, 695, 909
EcoNI CCTNNNNNAGG 1 cut(s) 789
EcoO109I RGGNCCY 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 173, 763
EcoT22I ATGCAT 1 cut(s) 580
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 1 cut(s) 582
FaiI YATR 7 cut(s) 366, 534, 580, 683, 726, 743, 946
FaqI GGGAC 2 cut(s) 401, 571
FatI CATG 1 cut(s) 578
Fnu4HI GCNGC 2 cut(s) 6, 14
FokI GGATG 3 cut(s) 176, 549, 554
Fsp4HI GCNGC 2 cut(s) 6, 14
FspBI CTAG 3 cut(s) 357, 408, 489
GlaI GCGC 1 cut(s) 517
GluI GCNGC 2 cut(s) 6, 14
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 2 cut(s) 826, 849
HaeIII GGCC 2 cut(s) 178, 763
HapII CCGG 2 cut(s) 378, 662
HhaI GCGC 1 cut(s) 518
Hin1II CATG 1 cut(s) 582
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 4 cut(s) 497, 652, 690, 796
HpaI GTTAAC 1 cut(s) 565
HpaII CCGG 2 cut(s) 378, 662
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 3 cut(s) 29, 54, 502
Hpy188III TCNNGA 7 cut(s) 58, 386, 434, 730, 828, 843, 887
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 45, 206
HpyCH4III ACNGT 2 cut(s) 562, 978
HpyCH4V TGCA 1 cut(s) 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 863, 872
HpyF3I CTNAG 4 cut(s) 26, 46, 204, 255
Hsp92II CATG 1 cut(s) 582
HspAI GCGC 1 cut(s) 516
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 2 cut(s) 54, 847
LmnI GCTCC 5 cut(s) 13, 55, 321, 635, 808
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 4 cut(s) 565, 647, 902, 919
MaeI CTAG 3 cut(s) 357, 408, 489
MaeIII GTNAC 4 cut(s) 250, 348, 454, 880
MalI GATC 2 cut(s) 56, 849
MboI GATC 2 cut(s) 54, 847
MboII GAAGA 5 cut(s) 30, 304, 307, 404, 610
MflI RGATCY 1 cut(s) 847
MhlI GDGCHC 1 cut(s) 326
MluCI AATT 3 cut(s) 336, 550, 721
MlyI GAGTC 1 cut(s) 684
MmeI TCCRAC 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 580
MroXI GAANNNNTTC 1 cut(s) 34
MseI TTAA 5 cut(s) 195, 564, 678, 698, 969
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 2 cut(s) 378, 662
MspR9I CCNGG 2 cut(s) 175, 765
MvaI CCWGG 2 cut(s) 175, 765
MwoI GCNNNNNNNGC 3 cut(s) 476, 863, 872
NdeII GATC 2 cut(s) 54, 847
NlaIII CATG 1 cut(s) 582
NlaIV GGNNCC 2 cut(s) 810, 835
NmuCI GTSAC 2 cut(s) 454, 880
NsiI ATGCAT 1 cut(s) 580
NspI RCATGY 1 cut(s) 582
PaeI GCATGC 1 cut(s) 582
PceI AGGCCT 1 cut(s) 763
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 3 cut(s) 497, 652, 796
PkrI GCNGC 2 cut(s) 7, 15
PleI GAGTC 1 cut(s) 684
PpsI GAGTC 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 173, 763
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 2 cut(s) 173, 763
PspN4I GGNNCC 2 cut(s) 810, 835
PspPI GGNCC 1 cut(s) 177
PstNI CAGNNNCTG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 847
RsaI GTAC 1 cut(s) 943
RsaNI GTAC 1 cut(s) 942
SaqAI TTAA 5 cut(s) 195, 564, 678, 698, 969
SatI GCNGC 2 cut(s) 6, 14
Sau3AI GATC 2 cut(s) 54, 847
Sau96I GGNCC 1 cut(s) 177
SchI GAGTC 1 cut(s) 684
ScrFI CCNGG 2 cut(s) 175, 765
SduI GDGCHC 1 cut(s) 326
SfaNI GCATC 4 cut(s) 565, 647, 902, 919
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SpeI ACTAGT 2 cut(s) 407, 488
SphI GCATGC 1 cut(s) 582
Sse9I AATT 3 cut(s) 336, 550, 721
SseBI AGGCCT 1 cut(s) 763
SsiI CCGC 2 cut(s) 11, 857
SspMI CTAG 3 cut(s) 357, 408, 489
StuI AGGCCT 1 cut(s) 763
StyD4I CCNGG 2 cut(s) 173, 763
TaaI ACNGT 2 cut(s) 562, 978
TaqI TCGA 2 cut(s) 573, 870
TasI AATT 3 cut(s) 336, 550, 721
TfiI GAWTC 3 cut(s) 497, 652, 796
Tru1I TTAA 5 cut(s) 195, 564, 678, 698, 969
Tru9I TTAA 5 cut(s) 195, 564, 678, 698, 969
TscAI CASTG 3 cut(s) 232, 331, 928
TseFI GTSAC 2 cut(s) 454, 880
TseI GCWGC 2 cut(s) 5, 13
Tsp45I GTSAC 2 cut(s) 454, 880
TspDTI ATGAA 2 cut(s) 249, 795
TspGWI ACGGA 2 cut(s) 65, 130
TspRI CASTG 3 cut(s) 232, 331, 928
XagI CCTNNNNNAGG 1 cut(s) 789
XceI RCATGY 1 cut(s) 582
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 3 cut(s) 357, 408, 489
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.