Rroxscaffold_1G00062930

protein At2g34460, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008667
Physical Location & Seq
Reverse (-)
84960202 .. 84962852
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062930.1

Sequence Viewer

Length: 990 bp
ATGGCAGCTCCGCTGCCTCTCATTCTCAGAAACCCTGCTTCTCTCTTAGCTCCGATCCCGACGAACCCTCCTCTCCGTCTCCCCAACACTCATTCCCACATCCACTCCCCTCTCTCCACAACCAAACCCTTTTCCGTCATTTCACTCTCCTCCTCCTCCACCACCAGGCCCTTATTCCCCATCCTTAACTCCTCTAAGATGGAAGGGAGTGAAACCACTGGGAATGAAATCACAGAGGAAGTTACTGAGACCACAACTGAAGCAGCAAGTGTGAATGGTTTGAAGAAGATTTTTGTGGCTGGTGCTACTGGGAGCACCGGTAAAAGAATTGTTGAGCAGTTACTGGCTAGGGGTTATGGAGTAAAAGCTGGAGTTCGGGACTTGGAAAAAGCCAAAACTAGTCTTCCAAGCAACAACCCAGCTCTTGAAATTGTGAAAGCAGATGTGACAGAGGGTTCTGCGAAGTTGGCAGAAGCAATTAGTAGTGATTCGGAAGCTGTGATTTGCGCGACGGGGTTTAGCTATGGATGGGATGTGTTTGCTCCATGGAAGGTTGATAATTTTGGGACAGTTAACCTTGTCGATGCATGCCGTAAAGTTGGTGTGAAGAGGTTTATTCTGGTCAGTTCCATTTTAGTCAATGGAGCATCAATGGGGCAGATTCTCAATCCGGCTTACATTTTTCTTAATATATTTGGACTCACTTTAATCGCAAAGCTTCAGGCAGAGAATTATATCAGGAAGTCTGGTATAAACTACACAATAATAAGGCCTGGTGGGTTGAGAAATGAACCTCCAACAGGGAATCTTGTGATGGAGCCAGAGGACACTCTATCTGGAGGCACCATCTCCAGAGATCTGGTTGCGGAAGTAGCTATCGAGGCATTGGTCACTCCTGAAGCATCTTACAAGGTAGTTGAGATAGTTTCCAGTGCTGATTCCCCCAAGCGTACATACGAGGAACTGTTTGGTTCGGTTAAGCAACGGTGA

Protein Analysis

329

Amino Acids

35.08

Weight (kDa)

6.86

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 95 - 256 2.6e-18 NmrA-like family
Epimerase PF01370 97 - 258 2.2e-12 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 99 - 221 1.1e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 101 - 298 4.1e-45 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 842
AccII CGCG 1 cut(s) 509
AciI CCGC 2 cut(s) 11, 866
AclWI GGATC 1 cut(s) 49
AcuI CTGAAG 3 cut(s) 279, 704, 918
AfaI GTAC 1 cut(s) 952
AfiI CCNNNNNNNGG 2 cut(s) 165, 800
AgeI ACCGGT 1 cut(s) 317
AgsI TTSAA 2 cut(s) 283, 428
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 2 cut(s) 164, 772
AluBI AGCT 8 cut(s) 8, 50, 368, 422, 497, 522, 718, 875
AluI AGCT 8 cut(s) 8, 50, 368, 422, 497, 522, 718, 875
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 2 cut(s) 83, 242
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 343
AoxI GGCC 2 cut(s) 167, 770
ApeKI GCWGC 3 cut(s) 5, 13, 263
AsiGI ACCGGT 1 cut(s) 317
AspLEI GCGC 1 cut(s) 509
AspS9I GGNCC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 317
BbvI GCAGC 2 cut(s) 17, 275
BccI CCATC 5 cut(s) 188, 193, 522, 808, 854
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 2 cut(s) 166, 774
BcoDI GTCTC 2 cut(s) 83, 242
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 2 cut(s) 348, 399
BglII AGATCT 1 cut(s) 856
BisI GCNGC 3 cut(s) 6, 14, 264
BlsI GCNGC 3 cut(s) 7, 15, 265
Bme1390I CCNGG 2 cut(s) 166, 774
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 819, 844
BmrFI CCNGG 2 cut(s) 166, 774
BmrI ACTGGG 2 cut(s) 228, 318
BmsI GCATC 3 cut(s) 574, 656, 911
BmuI ACTGGG 2 cut(s) 228, 318
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 3 cut(s) 390, 835, 858
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 545
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 6 cut(s) 52, 82, 89, 119, 304, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 800
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 4 cut(s) 223, 313, 348, 930
BseBI CCWGG 2 cut(s) 166, 774
BseDI CCNNGG 1 cut(s) 545
BseGI GGATG 4 cut(s) 99, 180, 533, 538
BseLI CCNNNNNNNGG 2 cut(s) 165, 800
BseMII CTCAG 2 cut(s) 40, 237
BseNI ACTGG 4 cut(s) 223, 313, 348, 930
BseRI GAGGAG 5 cut(s) 60, 139, 142, 145, 181
BseXI GCAGC 2 cut(s) 17, 275
BseYI CCCAGC 1 cut(s) 418
Bsh1236I CGCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 169, 772
BshNI GGYRCC 1 cut(s) 842
BshTI ACCGGT 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 317
BsiSI CCGG 2 cut(s) 318, 671
BslFI GGGAC 2 cut(s) 392, 580
BslI CCNNNNNNNGG 2 cut(s) 165, 800
BsmAI GTCTC 2 cut(s) 83, 242
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 392, 580
BsnI GGCC 2 cut(s) 169, 772
Bso31I GGTCTC 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 2 cut(s) 54, 856
Bsp19I CCATGG 1 cut(s) 545
BspACI CCGC 2 cut(s) 11, 866
BspANI GGCC 2 cut(s) 169, 772
BspCNI CTCAG 2 cut(s) 39, 238
BspFNI CGCG 1 cut(s) 509
BspLI GGNNCC 2 cut(s) 819, 844
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 4 cut(s) 223, 313, 348, 930
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 1 cut(s) 545
BssMI GATC 2 cut(s) 54, 856
BssT1I CCWWGG 1 cut(s) 545
Bst2UI CCWGG 2 cut(s) 166, 774
Bst4CI ACNGT 3 cut(s) 571, 966, 987
Bst6I CTCTTC 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 4 cut(s) 26, 46, 195, 246
BstDSI CCRYGG 1 cut(s) 545
BstENI CCTNNNNNAGG 1 cut(s) 798
BstF5I GGATG 4 cut(s) 99, 180, 533, 538
BstFNI CGCG 1 cut(s) 509
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 2 cut(s) 57, 859
BstMAI GTCTC 2 cut(s) 83, 242
BstMBI GATC 2 cut(s) 54, 856
BstMWI GCNNNNNNNGC 3 cut(s) 467, 872, 881
BstNI CCWGG 2 cut(s) 166, 774
BstNSI RCATGY 1 cut(s) 591
BstSCI CCNGG 2 cut(s) 164, 772
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 2 cut(s) 17, 275
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 1 cut(s) 856
BstXI CCANNNNNNTGG 1 cut(s) 859
BstYI RGATCY 1 cut(s) 856
BsuRI GGCC 2 cut(s) 169, 772
BtgI CCRYGG 1 cut(s) 545
BtsCI GGATG 4 cut(s) 99, 180, 533, 538
BtsIMutI CAGTG 2 cut(s) 216, 937
Cac8I GCNNGC 1 cut(s) 589
CaiI CAGNNNCTG 1 cut(s) 343
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 951
CspAI ACCGGT 1 cut(s) 317
CviAII CATG 2 cut(s) 546, 588
CviQI GTAC 1 cut(s) 951
DdeI CTNAG 4 cut(s) 26, 46, 195, 246
DpnI GATC 2 cut(s) 56, 858
DpnII GATC 2 cut(s) 54, 856
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
Eco130I CCWWGG 1 cut(s) 545
Eco147I AGGCCT 1 cut(s) 772
Eco31I GGTCTC 1 cut(s) 242
Eco57I CTGAAG 3 cut(s) 279, 704, 918
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 164, 772
EcoT14I CCWWGG 1 cut(s) 545
EcoT22I ATGCAT 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 545
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 2 cut(s) 549, 591
FaiI YATR 8 cut(s) 357, 525, 547, 589, 692, 735, 752, 955
FaqI GGGAC 2 cut(s) 392, 580
FatI CATG 2 cut(s) 545, 587
Fnu4HI GCNGC 3 cut(s) 6, 14, 264
FokI GGATG 4 cut(s) 86, 167, 540, 545
Fsp4HI GCNGC 3 cut(s) 6, 14, 264
FspBI CTAG 2 cut(s) 348, 399
GlaI GCGC 1 cut(s) 508
GluI GCNGC 3 cut(s) 6, 14, 264
GsaI CCCAGC 1 cut(s) 422
GsuI CTGGAG 3 cut(s) 390, 835, 858
HaeIII GGCC 2 cut(s) 169, 772
HapII CCGG 2 cut(s) 318, 671
HhaI GCGC 1 cut(s) 509
Hin1II CATG 2 cut(s) 549, 591
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 5 cut(s) 488, 661, 699, 805, 938
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 2 cut(s) 318, 671
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 3 cut(s) 29, 54, 493
Hpy188III TCNNGA 7 cut(s) 58, 377, 425, 739, 837, 852, 896
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 2 cut(s) 64, 514
HpyAV CCTTC 2 cut(s) 197, 544
HpyCH4III ACNGT 3 cut(s) 571, 966, 987
HpyCH4V TGCA 1 cut(s) 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 872, 881
HpyF3I CTNAG 4 cut(s) 26, 46, 195, 246
Hsp92II CATG 2 cut(s) 549, 591
HspAI GCGC 1 cut(s) 507
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 2 cut(s) 54, 856
LmnI GCTCC 6 cut(s) 13, 55, 312, 547, 644, 817
Lsp1109I GCAGC 2 cut(s) 17, 275
LweI GCATC 3 cut(s) 574, 656, 911
MaeI CTAG 2 cut(s) 348, 399
MaeIII GTNAC 4 cut(s) 241, 339, 445, 889
MalI GATC 2 cut(s) 56, 858
MboI GATC 2 cut(s) 54, 856
MboII GAAGA 4 cut(s) 295, 298, 395, 619
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 1 cut(s) 317
MluCI AATT 5 cut(s) 327, 429, 477, 559, 730
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 1 cut(s) 821
Mph1103I ATGCAT 1 cut(s) 589
MseI TTAA 5 cut(s) 186, 573, 687, 707, 978
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 2 cut(s) 318, 671
MspR9I CCNGG 2 cut(s) 166, 774
MvaI CCWGG 2 cut(s) 166, 774
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 467, 872, 881
NcoI CCATGG 1 cut(s) 545
NdeII GATC 2 cut(s) 54, 856
NlaIII CATG 2 cut(s) 549, 591
NlaIV GGNNCC 2 cut(s) 819, 844
NmuCI GTSAC 2 cut(s) 445, 889
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 591
PaeI GCATGC 1 cut(s) 591
PceI AGGCCT 1 cut(s) 772
PfeI GAWTC 4 cut(s) 488, 661, 805, 938
PinAI ACCGGT 1 cut(s) 317
PkrI GCNGC 3 cut(s) 7, 15, 265
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
Psp6I CCWGG 2 cut(s) 164, 772
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 2 cut(s) 164, 772
PspN4I GGNNCC 2 cut(s) 819, 844
PspPI GGNCC 1 cut(s) 168
PstNI CAGNNNCTG 1 cut(s) 343
PsuI RGATCY 1 cut(s) 856
RsaI GTAC 1 cut(s) 952
RsaNI GTAC 1 cut(s) 951
SaqAI TTAA 5 cut(s) 186, 573, 687, 707, 978
SatI GCNGC 3 cut(s) 6, 14, 264
Sau3AI GATC 2 cut(s) 54, 856
Sau96I GGNCC 1 cut(s) 168
SchI GAGTC 1 cut(s) 693
ScrFI CCNGG 2 cut(s) 166, 774
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 3 cut(s) 574, 656, 911
SpeI ACTAGT 1 cut(s) 398
SphI GCATGC 1 cut(s) 591
Sse9I AATT 5 cut(s) 327, 429, 477, 559, 730
SseBI AGGCCT 1 cut(s) 772
SsiI CCGC 2 cut(s) 11, 866
SspMI CTAG 2 cut(s) 348, 399
StuI AGGCCT 1 cut(s) 772
StyD4I CCNGG 2 cut(s) 164, 772
StyI CCWWGG 1 cut(s) 545
TaaI ACNGT 3 cut(s) 571, 966, 987
TaqI TCGA 2 cut(s) 582, 879
TasI AATT 5 cut(s) 327, 429, 477, 559, 730
TfiI GAWTC 4 cut(s) 488, 661, 805, 938
Tru1I TTAA 5 cut(s) 186, 573, 687, 707, 978
Tru9I TTAA 5 cut(s) 186, 573, 687, 707, 978
TscAI CASTG 2 cut(s) 223, 937
TseFI GTSAC 2 cut(s) 445, 889
TseI GCWGC 3 cut(s) 5, 13, 263
Tsp45I GTSAC 2 cut(s) 445, 889
TspDTI ATGAA 2 cut(s) 240, 804
TspGWI ACGGA 2 cut(s) 65, 124
TspRI CASTG 2 cut(s) 223, 937
XagI CCTNNNNNAGG 1 cut(s) 798
XceI RCATGY 1 cut(s) 591
XspI CTAG 2 cut(s) 348, 399
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.